miRNA-143 expression is associated with inflammation and time of exposure to amniotic fluid in experimental gastroschisis

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Tipo de produção
article
Data de publicação
2023
DOI
Scopus
Web of Science
PubMed
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ISSN da Revista
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Editora
ELSEVIER ESPANA
Autores
DINIZ, Ana Maria Bicudo
GUALBERTO, Igor Jose Nogueira
LIMA, Luiza Almeida
CIRINO, Mucio Luiz de Assis
MURAKAMI, Rodrigo Kendi
ISHIKIRIAMA, Bella Luna Colombini
RUANO, Rodrigo
TIRAPELLI, Daniela
SBRAGIA, Loureneo
Citação
CLINICS, v.78, article ID 100311, 7p, 2023
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Objective: Gastroschisis (GS) is a congenital anomaly in the abdominal wall with the intestinal loops exiting laterally to the umbilicus. The contact of the loops with Amniotic Fluid (AF) causes an inflammatory process in the exposed part, leading to an extended hospital stay and an increased risk of morbidity due to alterations related to intestinal motility. The authors aimed to evaluate the time of exposure to the AF in the experimental GS and to search for potential biomarkers of intestinal inflammation by measuring microRNAs.Methods: Rat fetuses were divided into three groups: a) CONTROL, b) GS reared on day 18 (GS = 18), and c) GS reared on day 19.5 (GS = 19) (term = 22 days). On day 21.5, the fetuses were removed for biometric parameters and biochemical analyses: 1) Biometrics: Body and Intestinal Weight (BW, IW), and intestinal-body weight ratio (IW/ BW); 2) Descriptive histopathology and 3) miR-143 quantification by real-time Polymerase Chain Reaction (PCR).Results: BW was higher in CONTROL than GS 18 and G19 (p < 0.05). IW, IW/BW, intestinal water, and mRNA-143 were higher in GS 18 and GS 19 than in CONTROL, and GS 18 was higher than GS 19 (p < 0.05). The average of the inflammation score from the intestinal wall with mucosal inflammation and intra-epithelial lymphocytes shows worst in GS 18 and GS 19 vs. CONTROL (p < 0.05).Conclusions: The tissue expression of mRNA-143 and the morphological changes in the intestine of GS worsened according to the time of exposure to AF, which could be a possible marker of fetal intestinal damage.
Palavras-chave
Gastroschisis, Rat, mRNA-143, Inflammation, Amniotic fluid
URI
https://observatorio.fm.usp.br/handle/OPI/58030
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/05