CAROLINE CRISTIANO REAL GREGORIO
Projetos de Pesquisa
Unidades Organizacionais
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Cannabidiol Treatment Improves Glucose Metabolism and Memory in Streptozotocin-Induced Alzheimer's Disease Rat Model: A Proof-of-Concept Study(2022) FARIA, Daniele de Paula; SOUZA, Larissa Estessi de; DURAN, Fabio Luis de Souza; BUCHPIGUEL, Carlos Alberto; BRITTO, Luiz Roberto; CRIPPA, Jose Alexandre de Souza; FILHO, Geraldo Busatto; REAL, Caroline CristianoAn early and persistent sign of Alzheimer's disease (AD) is glucose hypometabolism, which can be evaluated by positron emission tomography (PET) with F-18-2-fluoro-2-deoxy-D-glucose ([F-18]FDG). Cannabidiol has demonstrated neuroprotective and anti-inflammatory properties but has not been evaluated by PET imaging in an AD model. Intracerebroventricular (icv) injection of streptozotocin (STZ) is a validated model for hypometabolism observed in AD. This proof-of-concept study evaluated the effect of cannabidiol treatment in the brain glucose metabolism of an icv-STZ AD model by PET imaging. Wistar male rats received 3 mg/kg of STZ and [F-18]FDG PET images were acquired before and 7 days after STZ injection. Animals were treated with intraperitoneal cannabidiol (20 mg/kg-STZ-cannabidiol) or saline (STZ-saline) for one week. Novel object recognition was performed to evaluate short-term and long-term memory. [F-18]FDG uptake in the whole brain was significantly lower in the STZ-saline group. Voxel-based analysis revealed a hypometabolism cluster close to the lateral ventricle, which was smaller in STZ-cannabidiol animals. The brain regions with more evident hypometabolism were the striatum, motor cortex, hippocampus, and thalamus, which was not observed in STZ-cannabidiol animals. In addition, STZ-cannabidiol animals revealed no changes in memory index. Thus, this study suggests that cannabidiol could be an early treatment for the neurodegenerative process observed in AD.
- Development of a platform for the production of multiple modal chelating and imaging agents using desferrioxamine and bovine albumin as a model(2021) CORREIA, Lucas Antonio Arias; GARCIA, Rodrigo Santos; REAL, Caroline Cristiano; UZUELI, Daniel Henrique; SA, Ulisses Lacerda de Figueiredo; MARQUES, Fabio Luiz Navarro; COLNAGO, Luiz Alberto; ESPOSITO, Breno PanniaThe linker p-SCN-Bz-desferrioxamine was used as a conjugating agent to bovine serum albumin. The conjugate (BSA-DFO) did not display relevant structural alterations in comparison with the native protein. BSA-DFO was able to bind iron in a high affinity, antioxidant form similar to the native siderophore desferrioxamine. The gadolinium complex of BSA-DFO displayed 7-10 times higher relaxivities compared to the low molecular weight complex. Preparation of the radiotracers(68)Ga(BSA-DFO) and(89)Zr(BSA-DFO) in high radiochemical yields was accomplished quickly in mild conditions. These results indicate that BSA-DFO might be useful as an alternative treatment for iron overload disorders, and as a platform for the production of radiotracers or contrast agents for magnetic resonance imaging.
- Therapeutic Efficiency of Multiple Applications of Magnetic Hyperthermia Technique in Glioblastoma Using Aminosilane Coated Iron Oxide Nanoparticles: In Vitro and In Vivo Study(2020) REGO, Gabriel N. A.; NUCCI, Mariana P.; MAMANI, Javier B.; OLIVEIRA, Fernando A.; MARTI, Luciana C.; FILGUEIRAS, Igor S.; FERREIRA, Joao M.; REAL, Caroline C.; FARIA, Daniele de Paula; ESPINHA, Paloma L.; FANTACINI, Daianne M. C.; SOUZA, Lucas E. B.; COVAS, Dimas T.; BUCHPIGUEL, Carlos A.; GAMARRA, Lionel F.Magnetic hyperthermia (MHT) has been shown as a promising alternative therapy for glioblastoma (GBM) treatment. This study consists of three parts: The first part evaluates the heating potential of aminosilane-coated superparamagnetic iron oxide nanoparticles (SPIONa). The second and third parts comprise the evaluation ofMHT multiple applications in GBM model, either in vitro or in vivo. The obtained heating curves of SPIONa (100 nm, +20 mV) and their specific absorption rates (SAR) stablished the best therapeutic conditions for frequencies (309 kHz and 557 kHz) and magnetic field (300 Gauss), which were stablished based on three in vitro MHT application in C6 GBM cell line. The bioluminescence (BLI) signal decayed in all applications and parameters tested and 309 kHz with 300 Gauss have shown to provide the best therapeutic effect. These parameters were also established for three MHT applications in vivo, in which the decay of BLI signal correlates with reduced tumor and also with decreased tumor glucose uptake assessed by positron emission tomography (PET) images. The behavior assessment showed a slight improvement after each MHT therapy, but after three applications the motor function displayed a relevant and progressive improvement until the latest evaluation. Thus, MHT multiple applications allowed an almost total regression of the GBM tumor in vivo. However, futher evaluations after the therapy acute phase are necessary to follow the evolution or tumor total regression. BLI, positron emission tomography (PET), and spontaneous locomotion evaluation techniques were effective in longitudinally monitoring the therapeutic effects of the MHT technique.
- Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula(2021) BERGER, Arthur; ARAUJO-FILHO, Irami; PIFFOUX, Max; NICOLAS-BOLUDA, Alba; GRANGIER, Alice; BOUCENNA, Imane; REAL, Caroline Cristiano; MARQUES, Fabio Luiz Navarro; FARIA, Daniele de Paula; REGO, Amalia Cinthia Meneses do; BROUDIN, Chloe; GAZEAU, Florence; WILHELM, Claire; CLEMENT, Olivier; CELLIER, Christophe; BUCHPIGUEL, Carlos Alberto; RAHMI, Gabriel; SILVA, Amanda K. A.Extracellular vesicles (EVs), especially from stem/stromal cells (SCs), represent a cell-free alternative in regenerative medicine holding promises to promote tissue healing while providing safety and logistic advantages in comparison to cellular counterparts. Herein, we hypothesize that SC EVs, administered locally in a thermoresponsive gel, is a therapeutic strategy for managing post-surgical colo-cutaneous fistulas. This disease is a neglected and challenging condition associated to low remission rates and high refractoriness. Herein, EVs from a murine SC line were produced by a high-yield scalable method in bioreactors. The post-surgical intestinal fistula model was induced via a surgical cecostomy communicating the cecum and the skin in Wistar rats. Animals were treated just after cecostomy with PBS, thermoresponsive Pluronic F-127 hydrogel alone or containing SC EVs. A PET-monitored biodistribution investigation of SC EVs labelled with Zr-89 was performed. Fistula external orifice and output assessment, probe-based confocal laser endomicroscopy, MRI and histology were carried out for therapy follow-up. The relevance of percutaneous EV administration embedded in the hydrogel vehicle was indicated by the PET-biodistribution study. Local administration of SC EVs in the hydrogel reduced colo-cutaneous fistula diameter, output, fibrosis and inflammation while increasing the density of neo-vessels when compared to the PBS and gel groups. This multi-modal investigation pointed-out the therapeutic potential of SC EVs administered locally and in a thermoresponsive hydrogel for the management of challenging post-surgical colon fistulas in a minimally-invasive cell-free strategy.