ANA CAROLINA PRADO RIBEIRO E SILVA
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
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- Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer(2018) CARNIELLI, Carolina Moretto; MACEDO, Carolina Carneiro Soares; ROSSI, Tatiane De; GRANATO, Daniela Campos; RIVERA, Cesar; DOMINGUES, Romenia Ramos; PAULETTI, Bianca Alves; YOKOO, Sami; HEBERLE, Henry; BUSSO-LOPES, Ariane Fidelis; CERVIGNE, Nilva Karla; SAWAZAKI-CALONE, Iris; MEIRELLES, Gabriela Vaz; MARCHI, Fabio Albuquerque; TELLES, Guilherme Pimentel; MINGHIM, Rosane; RIBEIRO, Ana Carolina Prado; BRANDAO, Thais Bianca; CASTRO JR., Gilberto de; GONZALEZ-ARRIAGADA, Wilfredo Alejandro; GOMES, Alexandre; PENTEADO, Fabio; SANTOS-SILVA, Alan Roger; LOPES, Marcio Ajudarte; RODRIGUES, Priscila Campioni; SUNDQUIST, Elias; SALO, Tuula; SILVA, Sabrina Daniela da; ALAOUI-JAMALI, Moulay A.; GRANER, Edgard; FOX, Jay W.; COLETTA, Ricardo Della; LEME, Adriana Franco PaesDifferent regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor-node-metastasis prognosis classification. Therefore, defining biological signatures that allow assessing the prognostic outcomes for OSCC patients would be of great clinical significance. Using histopathology-guided discovery proteomics, we analyze neoplastic islands and stroma from the invasive tumor front (ITF) and inner tumor to identify differentially expressed proteins. Potential signature proteins are prioritized and further investigated by immunohistochemistry (IHC) and targeted proteomics. IHC indicates low expression of cystatin-B in neoplastic islands from the ITF as an independent marker for local recurrence. Targeted proteomics analysis of the prioritized proteins in saliva, combined with machine-learning methods, highlights a peptide-based signature as the most powerful predictor to distinguish patients with and without lymph node metastasis. In summary, we identify a robust signature, which may enhance prognostic decisions in OSCC and better guide treatment to reduce tumor recurrence or lymph node metastasis.
- Locally advanced oral squamous cell carcinoma patients treated with photobiomodulation for prevention of oral mucositis: retrospective outcomes and safety analyses(2018) BRANDAO, Thais Bianca; MORAIS-FARIA, Karina; RIBEIRO, Ana Carolina Prado; RIVERA, Cesar; SALVAJOLI, Joao Victor; LOPES, Marcio Ajudarte; EPSTEIN, Joel B.; ARANY, Praveen R.; CASTRO JR., Gilberto de; MIGLIORATI, Cesar Augusto; SANTOS-SILVA, Alan RogerThe well-established clinical efficacy of photobiomodulation (PBM) therapy in management of oral mucositis (OM) is leading to increasing use in oncology care. This protection and enhanced repair of damage to mucosal tissue have led to the question of the potential effects of PBM therapy on pre-malignant and malignant cells. The purpose of this study was to examine the outcome of cancer therapy and incidence of tumor recurrence in locally advanced oral squamous cell carcinoma (OSCC) patients treated with PBM therapy for OM. A retrospective clinical analysis of 152 advanced OSCC patients treated with prophylactic PBM therapy for radiotherapy-induced OM from January 2009 to December 2014 was conducted. Of the 152 OSCC patients treated with PBM therapy in this study, 19 (12.5%) had stage III and 133 (87.5%) had stage IV tumors. Of these, 52 (34.2%) received initial treatment with surgery followed by adjuvant radiotherapy, 94 (61.8%) with exclusive chemoradiation, and 6 (4%) with induction chemotherapy followed by surgery and radiotherapy. After a mean follow-up of 40.84 (+/- 11.71) months, the overall survival and disease-free survival rates were 46.7 and 51.8%, respectively. Forty-five (29.6%) patients developed local-regional recurrence, 10 (6.57%) patients developed distant relapse, and 19 (12.5%) developed new (second) primary tumors. Clinicopathological features and survival outcomes in the PBM-treated patients were similar to previously published data for conventional treatments in patients with advanced OSCC. In this study, prophylactic use of PBM therapy did not impact treatment outcomes of the primary cancer, recurrence or new primary tumors, or survival in advanced OSCC patients.
- Tongue metastasis of cutaneous melanoma: Report of two cases and literature review(2018) MARKMAN, R.-L.; ROSA, G.-A.; CARDILI, L.; SIMONATO, L.-E.; BRANDãO, T.-B.; RIBEIRO, A.-C.-P.Introduction: Malignant metastases to the oral cavity are rare and metastatic melanomas of the tongue are considered exceptionally uncommon, with less than 10 cases published in the English literature so far. Case reports: Two female patients in the 7th decade of life presented to our dental service with nodules in the tongue. Both patients had multiple metastases at the time of oral diagnosis and primary melanoma originated on the skin. An intra-oral incisional biopsy was performed under local anesthesia and the histopathologic analysis was characterized by the proliferation of atypical epithelioid cells displaying a poorly delimited cytoplasm and hyperchromatic nucleus which contained eosinophilic macronucleoli. Immunohistochemistry was performed in both cases to confirm the clinical hypothesis of metastatic melanoma. After the diagnosis of oral metastatic melanoma, the patients were maintained under palliative care and close medical follow-up. Both patients died four and a half months and 20 months after the diagnosis of tongue metastasis. Conclusions: Although rare, metastatic melanoma should be included in the differential diagnosis of tongue lesions detected in patients with a previous medical history of cutaneous melanoma. © Medicina Oral S. L. C.I.F.
- Cracked tooth syndrome in irradiated patients with head and neck cancer(2018) PALMIER, Natalia Rangel; MADRID, Cristhian Camilo; PAGLIONI, Mariana de Pauli; RIVERA, Cesar; MARTINS, Beatriz Nascimento F. Lebre; ARAUJO, Anna Luiza Damaccno; SALVAJOLI, Joao Victor; GOES, Mario Fernando de; LOPES, Marcio Ajudarte; RIBEIRO, Ana Carolina Prado; BRANDAO, Thais Bianca; SANTOS-SILVA, Alan RogerObjectives. The aim of this study was to assess the presence of enamel craze lines (ECLs), part of the spectrum of the so-called cracked tooth syndrome, on the surface of teeth irradiated in vivo. Study Design. Forty teeth extracted from patients with head and neck cancer were paired, matched, and equally divided into 4 groups: noncarious irradiated (G1); noncarious control (G2); radiation-related caries (RRC) (G3), and carious control (G4). Samples were examined for ECL detection with a fiberoptic transillumination device and photographed, and ECL mean size, number, and patterns of topographic distribution in tooth crown were determined. Groups were compared accordingly: G1 versus G2; G3 versus G4. Results. We analyzed 538 ECLs, of which 30.1% were found in noncarious irradiated teeth, 19.3% in noncarious controls, 27.6% in RRC, and 23% in carious controls. Non-carious irradiated teeth presented higher quantities of ECL than non-carious control (P < 0.05). Higher incidences of ECLs were identified in specific enamel topographies of anterior G1 and G3 samples (P <.05). There was no correlation between ECL size/numbers and radiation isodose delivery to teeth. Conclusions. Increased incidence of ECLs may indicate weakened enamel structure in irradiated teeth, and this may play a role in the onset and progression of RRC.
- Radiation-related superficial oral mucoceles: An under-recognized acute toxicity in head and neck cancer patients(2018) PRADO-RIBEIRO, Ana-Carolina; SANTOS-SILVA, Alan-Roger; FARIA, Karina-Morais; SILVA, Wagner-Gomes; SIMONATO, Luciana-Estevam; MOUTINHO, Karina; BRANDAO, Thais-BiancaBackground: Acute toxicity is usually defined as adverse changes occurring immediately or a short time after the start of oncological treatment. Material an Methods: Cross-sectional retrospective study performed with head and neck cancer patients who underwent radiotherapy from 2013 to 2016. Results: Ten (1.2%) patients developed SOMs during radiotherapy, most (80%) of which were men with a mean age of 59.5 years at diagnosis. SOMs mainly affected the floor of the mouth (60%) between the fourth and the sixth weeks of radiation therapy. All lesions were asymptomatic and spontaneously ruptured approximately 9 days after diagnosis. Conclusions: Although rare, SOMs may be regarded as an acute oral toxicity of head and neck radiotherapy.