ALEXANDRE BEZERRA DOS SANTOS
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
6 resultados
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- Infrared and confocal Raman spectroscopy to differentiate changes in the protein secondary structure in normal and abnormal thyroid tissues(2018) SOTO, Claudio A. Tellez; MEDEIROS-NETO, Lazaro P.; SANTOS, Laurita dos; SANTOS, Andre B. O.; FERREIRA, Isabelle; SINGH, Priyanka; CANEVARI, Renata A.; MARTIN, Airton A.Thirty thyroid samples of normal and abnormal tissues were analyzed by infrared and confocal Raman Spectroscopy. We studied the Amide I (1,720-1,580cm(-1)) spectral region in order to determine different components of the proteins' secondary structure in the samples. Peak positions of the Amide I bands were determined using the second derivative and Fourier self-deconvolution of infrared and Raman spectra. We obtained band areas corresponding to -turn, 3(10)-helix, -helix, -sheet, -turns, and side chains for the infrared and confocal Raman spectra by Gaussian fitting. Using the second derivative of the infrared and Raman spectra, we demonstrated the consistency of the vibrational assignments because compounds of low symmetry without an inversion center present coincident bands that differ only in intensity; hence, the infrared procedures to identify and assign the bands of the proteins' secondary structure in the Raman spectra are valid. The results obtained are supported by vibrational theory and indicate that infrared and confocal Raman spectroscopy are important, useful, and fast tools to identify changes in the proteins' secondary structure in normal tissues, goiter, and follicular and papillary thyroid carcinomas.
- Short-term survival in extensive craniofacial resections(2021) LEITE, Ana Kober N.; ALVARENGA, Gustavo Fernandes de; GONCALVES, Sergio; SANTOS, Alexandre Bezerra dos; NETO, Hugo Sterman; CERNEA, Claudio R.; V, Marco Aurelio Kulcsar; KOWALSKI, Luiz Paulo; MATOS, Leandro LuongoOBJECTIVES: Craniofacial resection (CFR) procedures for craniofacial tumors with cranial extension are often extensive. Although CFRs may yield good oncological results, there are concerns about high perioperative morbidity and mortality. This study aimed to determine risk factors for perioperative mortality after open CFR in terms of deaths occurring during index hospitalizations. METHODS: We conducted a retrospective analysis of CFRs conducted at a tertiary oncology hospital from May 2009 through December 2018. RESULTS: Our analysis included data from the medical records of 102 patients, the majority of whom were male (n=74, 72.5%). The mean age was 61 years (+/- 18.3 years). Skin malignancies (n=64, 63.4%) accounted for nearly two-thirds of the treated tumors, and most of these were squamous cell carcinoma. Postoperative medical complications occurred in 33 patients (33%), and surgical complications occurred in 48 (47%). Multivariate analysis revealed the only independent risk factors for perioperative deaths to be the presence of intracranial tumor extension on preoperative imaging (hazard ratio [HR]=4.56; 95% confidence interval [CI]: 1.74-11.97; p=0.002) and the unexpected emergence of postoperative neurological dysfunction (HR=10.9; 95% CI: 2.21-54.3; p=0.003). CONCLUSIONS: In our study, factors related to tumor extension were associated with a higher risk of perioperative death.
bookPart Estadiamento de tumores de laringe e hipofaringe(2022) SANTOS, Alexandre Bezerra dos- Low frequency of cigarette smoking and the risk of head and neck cancer in the INHANCE consortium pooled analysis(2016) BERTHILLER, Julien; STRAIF, Kurt; AGUDO, Antonio; AHRENS, Wolfgang; SANTOS, Alexandre Bezerra dos; BOCCIA, Stefania; CADONI, Gabriella; CANOVA, Cristina; CASTELLSAGUE, Xavier; CHEN, Chu; CONWAY, David; CURADO, Maria Paula; MASO, Luigino Dal; DAUDT, Alexander W.; FABIANOVA, Eleonora; FERNANDEZ, Leticia; FRANCESCHI, Silvia; FUKUYAMA, Erica E.; HAYES, Richard B.; HEALY, Claire; HERRERO, Rolando; HOLCATOVA, Ivana; KELSEY, Karl; KJAERHEIM, Kristina; KOIFMAN, Sergio; LAGIOU, Pagona; VECCHIA, Carlo La; LAZARUS, Philip; LEVI, Fabio; LISSOWSKA, Jolanta; MACFARLANE, Tatiana; MATES, Dana; MCCLEAN, Michael; MENEZES, Ana; MERLETTI, Franco; MORGENSTERN, Hal; MUSCAT, Joshua; OLSHAN, Andrew F.; PURDUE, Mark; RAMROTH, Heribert; RUDNAI, Peter; SCHWARTZ, Stephen M.; SERRAINO, Diego; SHANGINA, Oxana; SMITH, Elaine; STURGIS, Erich M.; SZESZENIA-DABROWSKA, Neonila; THOMSON, Peter; VAUGHAN, Thomas L.; VILENSKY, Marta; WEI, Qingyi; WINN, Deborah M.; WUNSCH-FILHO, Victor; ZHANG, Zuo-Feng; ZNAOR, Ariana; FERRO, Gilles; BRENNAN, Paul; BOFFETTA, Paolo; HASHIBE, Mia; LEE, Yuan-Chin AmyBackground: Cigarette smoking is a major risk factor for head and neck cancer (HNC). To our knowledge, low cigarette smoking (<10 cigarettes per day) has not been extensively investigated in fine categories or among never alcohol drinkers. Methods: We conducted a pooled analysis of individual participant data from 23 independent case-control studies including 19 660 HNC cases and 25 566 controls. After exclusion of subjects using other tobacco products including cigars, pipes, snuffed or chewed tobacco and straw cigarettes (tobacco product used in Brazil), as well as subjects smoking more than 10 cigarettes per day, 4093 HNC cases and 13 416 controls were included in the analysis. The lifetime average frequency of cigarette consumption was categorized as follows: never cigarette users, >0-3, >3-5, >5-10 cigarettes per day. Results: Smoking >0-3 cigarettes per day was associated with a 50% increased risk of HNC in the study population [ odds ratio (OR) = 1.52, 95% confidence interval (CI): (1.21, 1.90). Smoking >3-5 cigarettes per day was associated in each subgroup from OR = 2.01 (95% CI: 1.22, 3.31) among never alcohol drinkers to OR = 2.74 (95% CI: 2.01, 3.74) among women and in each cancer site, particularly laryngeal cancer (OR = 3.48, 95% CI: 2.40, 5.05). However, the observed increased risk of HNC for low smoking frequency was not found among smokers with smoking duration shorter than 20 years. Conclusion: Our results suggest a public health message that low frequency of cigarette consumption contributes to the development of HNC. However, smoking duration seems to play at least an equal or a stronger role in the development of HNC.
- Head and Neck Surgery(2022) SANTOS, A. B. dos; KOWALSKI, L. P.; KöHLER, H. F.; GOLDENBERG, D. C.; TOLEDO, R. N. de; CARVALHO, A. Y. deHead and neck region is very rich in vascular structures and a site for every incident of malignant neoplasms with potential to nodal metastasis in the carotid sheath that, when operated, may require complex vascular reconstruction with potential morbid complications, especially in salvage surgery. But the region can also be affected by complex benignant neoplasms that may require concomitant endovascular and open approaches for proper treatment, besides the complexity of surrounding anatomical structures, and life-threatening bleeding events. © Springer Nature Switzerland AG 2022.
- Recommendations for head and neck surgical procedures during the COVID-19 pandemic(2020) KULCSAR, Marco A. V.; MONTENEGRO, Fabio L. M.; SANTOS, Andre B. O.; TAVARES, Marcos R.; ARAP, Sergio S.; KOWALSKI, Luiz P.The coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread exponentially worldwide. In Brazil, the number of infected people diagnosed has been increasing and, as in other countries, it has been associated with a high risk of contamination in healthcare teams. For healthcare professionals, the full use of personal protective equipment (PPE) is mandatory, such as wearing surgical or filtering facepiece class 2 (FFP2) masks, waterproof aprons, gloves, and goggles, in addition to training in care processes. A reduction in the number of face-to-face visits and non-essential elective procedures is also recommended. However, surgery should not be postponed in the case of the most essential elective indications (mostly associated with head and neck cancers). As malignant tumors of the head and neck are clinically time sensitive, neither consultations for these tumors nor their treatment should be postponed. Postponing surgical treatment can result in a change in the disease stage and alter an individual's chance of survival. In this situation, planning of all treatments must begin with the request for, in addition to routine examinations, a nasal swab polymerase chain reaction for SARS-CoV-2 and chest computed tomography. Only if the results of these tests are positive or if fever or other symptoms suggestive of COVID-19 are present should the surgical procedure be postponed until the patient completely recovers. This is mandatory not only because of the risk of contamination of the surgical team but also because of the increased risk of postoperative complications and high risk of death. During this pandemic, the most effective safety measures are social distancing for the general public and the adequate availability and use of PPE in the healthcare field. The treatment of other chronic diseases, such as cancer, should be continued, as the damming of cases of these diseases will have a deleterious effect on the public healthcare system.