WELLINGTON LUIZ FERREIRA DA SILVA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/53 - Laboratório de Micologia, Hospital das Clínicas, Faculdade de Medicina
LIM/53 - Laboratório de Micologia, Hospital das Clínicas, Faculdade de Medicina
2 resultados
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- Human kidney damage in fatal dengue hemorrhagic fever results of glomeruli injury mainly induced by IL17(2016) PAGLIARI, Carla; QUARESMA, Juarez Antonio Simoes; KANASHIRO-GALO, Luciane; CARVALHO, Leda Viegas de; VITORIA, Webster Oliveira; SILVA, Wellington Luiz Ferreira da; PENNY, Ricardo; VASCONCELOS, Barbara Cristina Baldez; VASCONCELOS, Pedro Fernando da Costa; DUARTE, Maria Irma SeixasAcute kidney injury is an unusual complication during dengue infection. The objective of this study was to better identify the characteristics of glomerular changes focusing on in situ immune cells and cytokines. An immunohistochemical assay was performed on 20 kidney specimens from fatal human cases of dengue hemorrhagic fever (DHF). It was observed a lymphomononuclear infiltrate, neutrophils and nuclear fragmentation in the glomeruli, hydropic degeneration, nuclear retraction, eosinophilic tubules and intense acute congestion. Sickle erythrocytes were frequent in glomeruli and inflammatory infiltrate. The glomeruli presented endothelial swelling and mesangial proliferation. Lymphocytes CD4+ predominated over CD8+ T cells, B cells and natural killer cells. There were also an expressive number of macrophagic CD68+ cells. 5100, Foxp3 and CD123 cells were not identified. Cells expressing IL17 and IL18+ cytokines predominated in the renal tissues, while IL4, IL6, IL10, IL13, TNF-alpha and IFN-gamma were rarely visualized. The high number of cells expressing IL17 and IL18+ could reflect the acute inflammatory response and possibly contribute to the local lesion. CD8+ T cells could play a role in the cytotoxic response. DHF is a multifactorial disease of capillary leakage associated with a ""Tsunami of cytokines expression"". The large numbers of cells expressing IL17 seems to play a role favoring the increased permeability.
- In Situ Immune Response in Human Chromoblastomycosis - A Possible Role for Regulatory and Th17 T Cells(2014) SILVA, Aline Alves de Lima; CRIADO, Paulo Ricardo; NUNES, Ricardo Spina; SILVA, Wellington Luiz Ferreira da; KANASHIRO-GALO, Luciane; DUARTE, Maria Irma Seixas; SOTTO, Mirian N.; PAGLIARI, CarlaBackground: Chromoblastomycosis is a chronic fungal infection that affects skin and subcutaneous tissue. Lesions can be classified in tumorous, verrucous, cicatricial and plaque type. The cellular immune response in the severe form of the disease seems to correlate with a Th2 pattern of cytokines. The humoral immune response also seems to play a role. We intended to explore the populations of regulatory T cells and the Th17 pattern. Methodology: Twenty-three biopsies of verrucous form were obtained from patients with clinical, culture and histopathological diagnostic of chromoblastomycosis, without treatment. It was performed an immunohistochemistry method to detect Foxp3, CD25, TGF-beta, IL-6, IL-17 and IL-23. Principal findings: IL-17 was the only cytokine with high expression in CBM when compared to normal skin. The expression of Treg cells, TGF-beta, IL-6 and IL-23 were similar to normal skin. Conclusions/Significance: The constitution of a local immune response with high expression of IL-17 and low expression of other cytokines could be at least in part, an attempt to help the immune system against fungal infection. On the other hand, high levels of local immune response mediated by Th17 profile could overcome the role of Treg cells. The inefficient immunomodulation as a consequence of the unbalance by Treg/Th17 cells seems to corroborate with the less effective immune response against fungi.