VICTOR YUJI YARIWAKE
Projetos de Pesquisa
Unidades Organizacionais
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
12 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 12
- Inhaled ultrafine particles, epigenetics and systemic autoimmune rheumatic diseases(2020) FARHAT, Sylvia Costa Lima; YARIWAKE, Victor Yuji; VERAS, Mariana Matera; BRAGA, Alfesio Luis Ferreira; MALUF, Adriana Elias; SILVA, Clovis Artur
- Concentrated ambient fine particulate matter (PM2.5) exposure induce brain damage in pre and postnatal exposed mice(2020) DOMENICO, Marlise Di; BENEVENUTO, Sarah Gomes de Menezes; TOMASINI, Paula Pellenz; YARIWAKE, Victor Yuji; ALVES, Nilmara de Oliveira; RAHMEIER, Francine Luciano; FERNANDES, Marilda da Cruz; MOURA, Dinara Jaqueline; SALDIVA, Paulo Hilario Nascimento; VERAS, Mariana MateraAir pollution is a public health concern that has been associated with adverse effects on the development and functions of the central nervous system (CNS). However, studies on the effects of exposure to pollutants on the CNS across the entire developmental period still remain scarce. In this study, we investigated the impacts of prenatal and/or postnatal exposure to fine particulate matter (PM2.5) from Sao Paulo city, on the brain structure and behavior of juvenile male mice. BALB/c mice were exposed to PM2.5 concentrated ambient particles (CAP) at a daily concentration of 600 mu g/m(3) during the gestational [gestational day (GD) 1.5-18.5] and the postnatal periods [postnatal day (PND) 22-90] to filtered air (FA) in both periods (FA/FA), to CAP only in the postnatal period (FA/CAP), to CAP only in the gestational period (CAP/FA), and to CAP in both periods (CAP/CAP). Behavioral tests were performed when animals were at PND 30 and PND 90. Glial activation, brain volume, cortical neuron number, serotonergic and GABAergic receptors, as well as oxidative stress, were measured. Mice at PND 90 presented greater behavioral changes in the form of greater locomotor activity in the FA-CAP and CAP-CAP groups. In general, these same groups explored objects longer and the CAP-FA group presented anxiolytic behavior. There was no difference in total brain volume among groups, but a lower corpus callosum (CC) volume was observed in the CAP-FA group. Also, the CAP-CAP group presented an increase in microglia in the cortex and an increased in astrocytes in the cortex, CC, and C1A and dentate gyrus of hippocampus regions. Gene expression analysis showed a decrease in BDNF in the hippocampus of CAP-CAP group. Treatment of immortalized glial cells with non-cytotoxic doses of ambient PM2.5 increased micronuclei frequencies, indicating genomic instability. These findings highlight the potential for negative neurodevelopmental outcomes induced by exposure to moderate levels of PM2.5 in Sao Paulo city.
conferenceObject Lasting effects of prenatal marijuana exposure on the retina: an experimental study in mice(2017) ZANTUT, Paulo; TAKAHASHI, Walter; YARIWAKE, Victor; TORRES, Janaina; PECORA, Ricardo; SAFATLE, Angelica; VERAS, Mariana; DAMICO, Francisco- Maternal exposure to air pollution alters energy balance transiently according to gender and changes gut microbiota(2023) ZORDAO, Olivia Pizetta; CAMPOLIM, Clara Machado; YARIWAKE, Victor Yuji; CASTRO, Gisele; FERREIRA, Clilton Krauess de Oliveira; SANTOS, Andrey; NORBERTO, Sonia; VERAS, Mariana Matera; SAAD, Mario Jose Abdalla; SALDIVA, Paulo Hilario Nascimento; KIM, Young-Bum; PRADA, Patricia OliveiraIntroductionThe timing of maternal exposure to air pollution is crucial to define metabolic changes in the offspring. Here we aimed to determine the most critical period of maternal exposure to particulate matter (PM2.5) that impairs offspring's energy metabolism and gut microbiota composition. MethodsUnexposed female and male C57BL/6J mice were mated. PM2.5 or filtered air (FA) exposure occurred only in gestation (PM2.5/FA) or lactation (FA/PM2.5). We studied the offspring of both genders. ResultsPM(2.5) exposure during gestation increased body weight (BW) at birth and from weaning to young in male adulthood. Leptin levels, food intake, Agrp, and Npy levels in the hypothalamus were also increased in young male offspring. Ikbke, Tnf increased in male PM2.5/FA. Males from FA/PM2.5 group were protected from these phenotypes showing higher O-2 consumption and Ucp1 in the brown adipose tissue. In female offspring, we did not see changes in BW at weaning. However, adult females from PM2.5/FA displayed higher BW and leptin levels, despite increased energy expenditure and thermogenesis. This group showed a slight increase in food intake. In female offspring from FA/PM2.5, BW, and leptin levels were elevated. This group displayed higher energy expenditure and a mild increase in food intake. To determine if maternal exposure to PM2.5 could affect the offspring's gut microbiota, we analyzed alpha diversity by Shannon and Simpson indexes and beta diversity by the Linear Discriminant Analysis (LDA) in offspring at 30 weeks. Unlike males, exposure during gestation led to higher adiposity and leptin maintenance in female offspring at this age. Gestation exposure was associated with decreased alpha diversity in the gut microbiota in both genders. DiscussionOur data support that exposure to air pollution during gestation is more harmful to metabolism than exposure during lactation. Male offspring had an unfavorable metabolic phenotype at a young age. However, at an older age, only females kept more adiposity. Ultimately, our data highlight the importance of controlling air pollution, especially during gestation.
- NEGATIVE EFFECTS OF SMOKING MARIJUANA DURING PREGNANCY ON THE PLACENTA: EXPERIMENTAL EVIDENCE(2019) VERAS, Mariana; BENEVENUTO, Sarah; TORRES, Janaina; YARIWAKE, Victor; MORAES, Thamires; SILVA, Iran
- Chronic exposure to PM2.5 aggravates SLE manifestations in lupus-prone mice(2021) YARIWAKE, Victor Yuji; TORRES, Janaina Iannicelli; SANTOS, Amandda Rakell Peixoto dos; FREITAS, Sarah Cristina Ferreira; ANGELIS, Katia De; FARHAT, Sylvia Costa Lima; CAMARA, Niels Olsen Saraiva; VERAS, Mariana MateraBackground Air pollution causes negative impacts on health. Systemic lupus erythematosus (SLE) is an autoimmune disease with diverse clinical manifestations and multifactorial etiology. Recent studies suggest that air pollution can trigger SLE and induce disease activity. However, this association has not been deeply investigated. Thus, the aim of this study was to evaluate whether exposure to fine particulate matter (PM2.5) exacerbates SLE manifestations, focusing on renal complications, in a lupus-prone animal model. Female NZBWF1 mice were exposed daily to 600 mu g/m(3) of inhaled concentrated ambient particles (CAP) or filtered air (FA). Survival rate, body weight, weight of organs (kidney, spleen, thymus, liver and heart), blood cell count, proteinuria, kidney stereology, renal histopathology, gene expression and oxidative stress were analyzed. Results Female NZBW mice exposed to CAP showed decreased survival, increased circulating neutrophils, early onset of proteinuria and increased kidney weight with renal cortex enlargement when compared to NZBW mice exposed to FA. Conclusions This work shows that air pollution aggravates some SLE manifestations in lupus-prone mice. These results reinforce the need of reducing air pollutant levels in order to promote a better quality of life for individuals diagnosed with SLE.
- Prenatal exposure to Cannabis smoke induces early and lasting damage to the brain(2022) BENEVENUTO, Sarah G. M.; DOMENICO, Marlise D.; YARIWAKE, Victor Y.; DIAS, Clarissa Tavares; MENDES-DA-SILVA, Cristiano; ALVES, Nilmara de O.; CAUMO, Sofia Ellen da S.; VASCONCELLOS, Perola; MORAIS, Damila R.; CARDOSO, Marilia S.; IANICELLI, Janaina; WAKED, Dunia; DAVEY, Gavin P.; BOYLAN, Fabio; COSTA, Jose L.; VERAS, Mariana MateraCannabis is the most widely used illegal drug during pregnancy, however, the effects of gestational exposure to Cannabis smoke (CS) on the central nervous system development remain uncharacterised. This study investigates the effects of maternal CS inhalation on brain function in the offspring. Pregnant mice were exposed daily to 5 min of CS during gestational days (GD) 5.5-17.5. On GD 18.5 half of the dams were euthanized for foetus removal. The offspring from the remaining dams were euthanized on postnatal days (PND) 20 and 60 for evaluation. Brain volume, cortex cell number, SOX2, histone-H3, parvalbumin, NeuN, and BDNF immunoreac-tivity were assessed in all groups. In addition, levels of NeuN, CB1 receptor, and BDNF expression were assessed and cortical primary neurons from rats were treated with Cannabis smoke extract (CSE) for assessment of cell viability. We found that male foetuses from the CS exposed group had decreased brain volume, whereas mice at PND 60 from the exposed group presented with increased brain volume. Olfactory bulb and diencephalon vol-ume were found lower in foetuses exposed to CS. Mice at PND 60 from the exposed group had a smaller volume in the thalamus and hypothalamus while the cerebellum presented with a greater volume. Also, there was an increase in cortical BDNF immunoreactivity in CS exposed mice at PND 60. Protein expression analysis showed an increase in pro-BDNF in foetus brains exposed to CS. Mice at PND 60 presented an increase in mature BDNF in the prefrontal cortex (PFC) in the exposed group and a higher CB1 receptor expression in the PFC. Moreover, hippocampal NeuN expression was higher in adult animals from the exposed group. Lastly, treatment of cortical primary neurons with doses of CSE resulted in decreased cell viability. These findings highlight the potential negative neurodevelopmental outcomes induced by gestational CS exposure.
- Effect of chronic exposure to fine particulate matter on cardiac tissue of NZBWF1 mice(2023) WAKED, Dunia; RODRIGUES, Ana Clara B.; SILVA, Thamires Moraes; YARIWAKE, Victor Yuji; FARHAT, Sylvia Costa Lima; VERAS, Mariana MateraEpidemiological and toxicological studies have shown that inhalation of particulate matter (PM) is associated with development of cardiovascular diseases. Long-term exposure to PM may increase the risk of cardiovascular events and reduce life expectancy. Systemic lupus erythematosus (SLE) is a chronic inflammatory disease, autoimmune in nature, that is characterized by the production of autoantibodies that affects several organs, including the heart. Air pollution - which can be caused by several different factors - may be one of the most important points both at the onset and the natural history of SLE. Therefore this study aims to investigate whether exposure to air pollution promotes increased inflammation and cardiac remodelling in animals predisposed to SLE. Female NZBWF1 mice were exposed to an environmental particle concentrator. Aspects related to cardiac remodelling, inflammation and apoptosis were analysed in the myocardium. Body weight gain, cardiac trophism by heart/body weight ratio, relative area of cardiomyocytes and the fibrotic area of cardiac tissue were evaluated during the exposure period. Animals exposed to PM2.5 showed increased area of cardiomyocytes, and area of fibrosis; in addition, we observed an increase in IL-1 and C3 in the cardiac tissue, demonstrating increased inflammation. We suggest that air pollution is capable of promoting cardiac remodelling and increased inflammation in animals predisposed to SLE.
conferenceObject FINE INHALABLE PARTICULATE MATTER (PM2.5) PRESENT IN AIR POLLUTION AND ITS EFFECTS ON MALE GERM CELLS CHROMATIN PACKAGING.(2023) TAMASHIRO, Leticia Kaory; YARIWAKE, Victor Yuji; VERAS, Mariana Matera; BERTOLLA, Ricardo P.; INTASQUI, Paula- Effects of cannabis and its components on the retina: a systematic review(2020) ZANTUT, Paulo R. A.; VERAS, Mariana M.; YARIWAKE, Victor Y.; TAKAHASHI, Walter Y.; SALDIVA, Paulo H.; YOUNG, Lucy H.; DAMICO, Francisco Max; FAJERSZTAJN, LaisPurpose: Cannabis is the most prevalent drug in the world and its consumption is growing. Cannabinoid receptors are present in the human central nervous system. Recent studies show evidence of the effects of cannabinoids on the retina, and synthesising the results of these studies may be relevant for ophthalmologists. Thus, this review adopts standardised, systematic review methodology to investigate the effects of exposure to cannabis and components on the retina. Methods: We searched five online databases for the combined terms for outcome (""retina"") and exposure (""cannabis""). Eligibility of studies were conducted by two independent reviewers, and risk of bias was assessed. Results: We retrieved 495 studies, screened 229 studies, assessed 52 studies for eligibility, and included 16 studies for qualitative analysis. The cannabinoids most frequently investigated were delta-9-tetrahydrocannabinol (THC), abnormal cannabidiol, synthetic cannabinoid, and cannabidiol (CDB). The outcomes most studied were neuroretinal dysfunction, followed by vascular effects. The studies also included investigation of neuroprotective and anti-inflammatory effects and teratogenic effects. Conclusions: This review suggests that cannabinoids may have an important role in retinal processing and function.