NATHALIA MONTOURO PINHEIRO MENEGASSO
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
27 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 27
- Effects of Eugenol and Dehydrodieugenol B from Nectandra leucantha against Lipopolysaccharide (LPS)-Induced Experimental Acute Lung Inflammation(2021) I, Marcia Bittencourt-Mernak; PINHEIRO, Nathalia M.; SILVA, Rafael C. da; PONCI, Vitor; BANZATO, Rosana; PINHEIRO, Aruana J. M. C. R.; OLIVO, Clarice R.; TIBERIO, Iolanda F. L. C.; LIMA NETO, Lidio G.; SANTANA, Fernanda P. R.; LAGO, Joao H. G.; PRADO, Carla M.Acute lung injury (ALI) is an important public health problem. The present work investigated whether dehydrodieugenol B treatment, a compound isolated from Brazilian plant Nectandra leucantha (Lauraceae), modulates experimental ALI and compared the observed effects to eugenol. Effects of dehydrodieugenol B in vitro in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were evaluated. The lung and systemic inflammatory profile, lung function, and possible mechanisms involved in BALB/C male mice (6-8 weeks) with ALI induced by LPS instillation (5 mg/kg) was assayed. Dehydrodieugenol B did not affect the cell viability and inhibited the increase in NO release and IL-1 beta and IL-6 gene expression induced by LPS. In vivo, both compounds reduced lung edema, inflammatory cells, and the IL-6 and IL-1 beta levels in bronchoalveolar lavage fluid, as well as reduced inflammatory cell infiltration and those positive to iNOS, MMP-9, and TIMP-1, and reduced the collagen content and the 8-isoprostane expression in lung tissue. Eugenol and dehydrodieugenol B also inhibited the phosphorylation of Jc-Jun-NH2 terminal Kinase (JNK), a signaling protein involved in the MAPKinase pathway. There was no effect of these compounds in lung function. Therefore, eugenol and dehydrodieugenol B ameliorates several features of experimental ALI and could be considered as a pharmacological tool to ameliorate acute lung inflammation.
conferenceObject Plant proteinase from Bauhinia bauhinioides Kallikrein inhibitor (BbKI) attenuates mechanics, inflammation and remodelling induced by elastase in mice(2012) OLIVEIRA, Bruno Martins; ALMEIDA-REIS, Rafael; THEODORO, Osmar A.; OLIVA, Leandro V.; RODRIGUES, Daniel Flisch; PINHEIRO, Nathalia; OLIVA, Maria L. V.; PRADO, Carla M.; MARTINS, Milton M.; TIBERIO, Iolanda F. L. C.- Alpha-7 nicotinic receptor stimulation reduces airway inflammation in a murine model of asthma(2016) SANTANA, Fernanda Paula Roncon; MIRANDA, Claudia Jeane Claudino de Pontes; PINHEIRO, Nathalia Montouro; PERINI, Adenir; CAPERUTO, Luciana Chagas; TIBERIO, Iolanda de Fatima Lopes Calvo; PRADO, Marco Antonio Maximo; MARTINS, Milton de Arruda; PRADO, Vania Ferreira; PRADO, Carla Maximo
- Nicotinic alpha-7 receptor stimulation (alpha 7nAChR) inhibited NF-kB/STAT3/SOCS3 pathways in a murine model of asthma(2017) SANTANA, Fernanda Paula Roncon; TOMARI, Sergio Festa; MIRANDA, Claudia Jeane Claudino de Pontes; PINHEIRO, Nathalia Montouro; CAPERUTO, Luciana Chagas; TIBERIO, Iolanda de Fatima Lopes Calvo; PRADO, Marco Antonio Maximo; MARTINS, Milton de Arruda; PRADO, Vania Ferreira; PRADO, Carla Maximo
- Effects of VAChT reduction on the time course of lung inflammation in mice with acute lung injury(2016) PINHEIRO, Nathalia; SANTANA, Fernanda; CAPERUTO, Luciana; TIBERIO, Iolanda; PRADO, Vania; PRADO, Marco Antonio; MARTINS, Milton; PRADO, Carla
- The role of monoterpenes derived from essential oils in lung alteration in a model of emphysema(2016) PEREIRA, Ellen; GUERREIRO, Marina; SANTANA, Fernanda; PINHEIRO, Nathalia; CAPELLO, Tabata; LOPES, Fernanda; OLIVO, Clarice; TIBERIO, Iolanda; MARTINS, Milton; LAGO, Joao; PRADO, Carla
- Evidences of Herbal Medicine-Derived Natural Products Effects in Inflammatory Lung Diseases(2016) SANTANA, Fernanda Paula R.; PINHEIRO, Nathalia M.; MERNAK, Marcia Isabel B.; RIGHETTI, Renato F.; MARTINS, Milton A.; LAGO, Joao H. G.; LOPES, Fernanda D. T. Q. dos Santos; TIBERIO, Iolanda F. L. C.; PRADO, Carla M.Pulmonary inflammation is a hallmark of many respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), and acute respiratory syndrome distress (ARDS). Most of these diseases are treated with anti-inflammatory therapy in order to prevent or to reduce the pulmonary inflammation. Herbal medicine-derived natural products have been used in folk medicine and scientific studies to evaluate the value of these compounds have grown in recent years. Many substances derived from plants have the biological effects in vitro and in vivo, such as flavonoids, alkaloids, and terpenoids. Among the biological activities of natural products derived from plants can be pointed out the anti-inflammatory, antiviral, antiplatelet, antitumor anti-allergic activities, and antioxidant. Although many reports have evaluated the effects of these compounds in experimental models, studies evaluating clinical trials are scarce in the literature. This review aims to emphasize the effects of these different natural products in pulmonary diseases in experimental models and in humans and pointing out some possible mechanisms of action.
conferenceObject Lung inflammation was attenuated by sakuranetin treatment in a model of acute lung injury(2014) MERNAK, Marcia; SANTANA, Fernanda; PINHEIRO, Nathalia; SARAIVA-RAMANHOLO, Beatriz; GRECCO, Simone; TIBERIO, Iolanda; MARTINS, Milton; LAGO, Joao; PRADO, Carla- Structurally Related Monoterpenes p-Cymene, Carvacrol and Thymol Isolated from Essential Oil from Leaves of Lippia sidoides Cham. (Verbenaceae) Protect Mice against Elastase-Induced Emphysema(2016) GAMES, Ellen; GUERREIRO, Marina; SANTANA, Fernanda R.; PINHEIRO, Nathalia M.; OLIVEIRA, Emerson A. de; LOPES, Fernanda D. T. Q. S.; OLIVO, Clarice R.; TIBERIO, Iolanda F. L. C.; MARTINS, Milton A.; LAGO, Joao Henrique G.; PRADO, Carla M.Background: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and inflammation. Natural products, such as monoterpenes, displayed anti-inflammatory and anti-oxidant activities and can be used as a source of new compounds to COPD treatment. Our aim was to evaluate, in an elastase-induced pulmonary emphysema in mice, the effects of and underlying mechanisms of three related natural monoterpenes (p-cymene, carvacrol and thymol) isolated from essential oil from leaves Lippia sidoides Cham. (Verbenaceae). Methods: Mices received porcine pancreatic elastase (PPE) and were treated with p-cymene, carvacrol, thymol or vehicle 30 min later and again on 7th, 14th and 28th days. Lung inflammatory profile and histological sections were evaluated. Results: In the elastase-instilled animals, the tested monoterpenes reduced alveolar enlargement, macrophages and the levels of IL-1 beta, IL-6, IL-8 and IL-17 in bronchoalveolar lavage fluid (BALF), and collagen fibers, MMP-9 and p-65-NF-kappa B-positive cells in lung parenchyma (p < 0.05). All treatments attenuated levels of 8-iso-PGF2 alpha but only thymol was able to reduced exhaled nitric oxide (p < 0.05). Conclusion: Monoterpenes p-cymene, carvacrol and thymol reduced lung emphysema and inflammation in mice. No significant differences among the three monoterpenes treatments were found, suggesting that the presence of hydroxyl group in the molecular structure of thymol and carvacrol do not play a central role in the anti-inflammatory effects.
- Prophylactic and therapeutic treatment with the flavonone sakuranetin ameliorates LPS-induced acute lung injury(2017) BITTENCOURT-MERNAK, Marcia Isabel; PINHEIRO, Nathalia M.; SANTANA, Fernanda P. R.; GUERREIRO, Marina P.; SARAIVA-ROMANHOLO, Beatriz M.; GRECCO, Simone S.; CAPERUTO, Luciana C.; FELIZARDO, Raphael J. F.; CAMARA, Niels O. S.; TIBERIO, Iolanda F. L. C.; MARTINS, Mlton A.; LAGO, Joao Henrique G.; PRADO, Carla M.Sakuranetin is the main isolate flavonoid from Baccharis retusa (Asteraceae) leaves and exhibits anti-inflammatory and antioxidative activities. Acute respiratory distress syndrome is an acute failure of the respiratory system for which effective treatment is urgently necessary. This study investigated the preventive and therapeutic effects of sakuranetin on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Animals were treated with intranasal sakuranetin 30 min before or 6 h after instillation of LPS. Twenty-four hours after ALI was induced, lung function, inflammation, macrophages population markers, collagen fiber deposition, the extent of oxidative stress, and the expression of matrix metalloprotease-9 (MMP-9), tissue inhibitor of MMP- 9 (TIMP-1) and NF-kB were evaluated. The animals began to show lung alterations 6 h after LPS instillation, and these changes persisted until 24 h after LPS administration. Preventive and therapeutic treatment with sakuranetin reduced the neutrophils in the peripheral blood and in the bronchial alveolar lavage. Sakuranetin treatment also reduced macrophage populations, particularly that of M1-like macrophages. In addition, sakurnaetin treatment reduced keratinocyte-derived chemokines (IL-8 homolog) and NF-kB levels, collagen fiber formation, MMM-9 and TIMP-1-positive cells, and oxidative stress in lung tissues compared with LPS animals treated with vehicle. Finally, sakuranetin treatment also reduced total protein, and the levels of TNF-alpha and IL-1 beta in the lung. This study shows that sakuranetin prevented and reduced pulmonary inflammation induced by LPS. Because sakuranetin modulates oxidative stress, the NF-kB pathway, and lung function, it may constitute a novel therapeutic candidate to prevent and treat ALI.
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