GISELLE PACIFICO SARTORI
Projetos de Pesquisa
Unidades Organizacionais
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina
4 resultados
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Agora exibindo 1 - 4 de 4
- Fake news and covid-19: a concern due to the low vaccine coverage in Brazil(2022) FUJITA, Dennis Minoru; NALI, Luiz Henrique da Silva; SARTORI, Giselle Pacifico; GALISTEO, Andres Jimenez; JR, Heitor Franco de Andrade; LUNA, Expedito Jose de AlbuquerqueThis study comprised the application of a survey in Sao Paulo, Brazil, in 2 different periods of 2020: the beginning of the covid-19 pandemic and the disease's first peak (from March to April, 100 interviews) to the time of stability in case fatality rates (from May to July, 100 interviews); the questionnaire included was composed of 14 multiple-choice questions to evaluate the importance of mass communication channels, including social media, and the level of importance attributed to preventive measures at the beginning of the pandemic. The changes in people's behavior, even in a group with more schooling, which initially considered preventive measures to be very important (91%) but, in the second survey, was reduced to 82%. The reinforcement of preventive measures to reduce cases and deaths by covid-19 in Brazil is urgent, allied to recommendations with clear information on the importance of vaccination to avoid low rates as the current situation of vaccine coverage for preventable diseases.
- COVID-19 and Influenza coinfection: The rise of Omicron and H3N2 in Brazil-2022(2022) FUJITA, Dennis Minoru; SOARES, Graciela dos Santos; SARTOR, Giselle Pacifico; NALI, Luiz Henrique da Silva
- Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation(2021) SARTORI, Giselle Pacifico; COSTA, Andrea da; MACARINI, Fernanda Lucio dos Santos; MARIANO, Douglas Oscar Ceolin; PIMENTA, Daniel Carvalho; SPENCER, Patrick Jack; NALI, Luiz Henrique da Silva; JR, Andres Jimenez GalisteoBackground: Tetanus toxin blocks the release of the inhibitory neurotransmitters in the central nervous system and causes tetanus and its main form of prevention is through vaccination. The vaccine is produced by inactivation of tetanus toxin with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potential immunogenic response and to reduce the post-vaccination side effects. Therefore, the aim of this study was to characterize the tetanus toxin structure after different doses of ionizing radiation of Co-60. Methods: Irradiated and native tetanus toxin was characterized by SDS PAGE in reducing and non-reducing conditions and MALD-TOF. Enzymatic activity was measured by FRET substrate. Also, antigenic properties were assessed by ELISA and Western Blot data. Results: Characterization analysis revealed gradual modification on the tetanus toxin structure according to doses increase. Also, fragmentation and possible aggregations of the protein fragments were observed in higher doses. In the analysis of peptide preservation by enzymatic digestion and mass spectrometry, there was a slight modification in the identification up to the dose of 4 kGy. At subsequent doses, peptide identification was minimal. The analysis of the enzymatic activity by fluorescence showed 35 % attenuation in the activity even at higher doses. In the antigenic evaluation, anti-tetanus toxin antibodies were detected against the irradiated toxins at the different doses, with a gradual decrease as the dose increased, but remaining at satisfactory levels. Conclusion: Ionizing radiation promoted structural changes in the tetanus toxin such as fragmentation and/or aggregation and attenuation of enzymatic activity as the dose increased, but antigenic recognition of the toxin remained at good levels indicating its possible use as an immunogen. However, studies of enzymatic activity of tetanus toxin irradiated with doses above 8 kGy should be further analyzed.
- Increase of immigrants in emerging countries: free public healthcare and vaccination as preventive measures in Brazil(2019) FUJITA, Dennis Minoru; SALVADOR, Felipe Scassi; DAMIAO, Giselle Pacifico Sartori; FIGUEIREDO, Gerusa Maria; NALI, Luiz Henrique da Silva