LUZIA NAOKO SHINOHARA FURUKAWA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina
13 resultados
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- Maternal high-sodium intake alters the responsiveness of the renin-angiotensin system in adult offspring(2012) RAMOS, Debora R.; COSTA, Nauilo L.; JANG, Karen L. L.; OLIVEIRA, Ivone B.; SILVA, Alexandre A. da; HEIMANN, Joel C.; FURUKAWA, Luzia N. S.Aims: The goal of the current study was to evaluate the impact of maternal sodium intake during gestation on the systemic and renal renin-angiotensin-aldosterone-system (RAAS) of the adult offspring. Main methods: Female Wistar rats were fed high- (HSD-8.0% NaCl) or normal-sodium diets (NSD-1.3% NaCl) from 8 weeks of age until the delivery of their first litter. After birth, the offspring received NSD. Tail-cuff blood pressure (TcBP) was measured in the offspring between 6 and 12 weeks of age. At 12 weeks of age, the offspring were subjected to either one week of HSD or low sodium diet (LSD-0.1 6%NaCl) feeding to evaluate RAAS responsiveness or to acute saline overload to examine sodium excretory function. Plasma (PRA) and renal renin content (RRC), serum aldosterone (ALDO) levels, and renal cortical and medullary renin mRNA expression levels were evaluated at the end of the study. Key findings: TcBP was higher among dams fed HSD, but no TcBP differences were observed among the offspring. Male offspring, however, exhibited increased TcBP after one week of HSD feeding, and this effect was independent of maternal diet. Increased RAAS responsiveness to the HSD and LSD was also observed in male offspring. The baseline levels of PRA. ALDO, and cortical and medullary renin gene expression were lower but the RRC levels were higher among HSD-fed male offspring (HSDoff). Conversely, female HSDoff showed reduced sodium excretion 4 h after saline overload compared with female NSDoff. Significance: High maternal sodium intake is associated with gender-specific changes in RAAS responsiveness among adult offspring.
conferenceObject Effect of an angiotensin II type 2 receptor agonist on myocardial hypertrophy and fibrosis induced by high salt diet(2017) DOPONA, Ellen Priscila Brito; ROCHA, Veronica Favoni; OLIVEIRA, Ivone Braga de; FURUKAWA, Luzia Naoko Shinohara; HEIMANN, Joel Claudio- Salt intake during pregnancy alters offspring's myocardial structure(2013) ALVES-RODRIGUES, E. N.; VERAS, M. M.; ROSA, K. T.; CASTRO, I. de; FURUKAWA, L. N. S.; OLIVEIRA, I. B.; SOUZA, R. M.; HEIMANN, J. C.Background and Aim: To evaluate the effects of low or high salt intake during pregnancy on left ventricle of adult male offspring. Methods and results: Low-(LS, 0.15%), normal-(NS, 1.3%) or high-salt (HS, 8% NaCl) diet was given to Wistar rats during pregnancy. During lactation all dams received NS as well as the offspring after weaning. To evaluate cardiac response to salt overload, 50% of each offspring group was fed a high-salt (hs, 4% NaCl) diet from the 21st to the 36th week of age (LShs, NShs, HShs). The remaining 50% was maintained on NS (LSns, NSns and HSns). Echocardiography was done at 20 and 30 weeks of age. Mean blood pressure (MBP), histology and left ventricular angiotensin II content (AII) were analyzed at 36 weeks of age. Interventricular septum, left ventricular posterior wall and relative wall thickness increased from the 20th to the 30th week of age only in HShs, cardiomyocyte mean volume was higher in HShs compared to NShs, LShs and HSns. AII and left ventricular fibrosis were not different among groups. Conclusions: HS during pregnancy programs adult male offspring to a blood pressure and angiotensin II independent concentric left ventricular hypertrophy, with no fibrosis, in response to a chronic high-salt intake.
conferenceObject Salt intake during pregnancy influences offspring organ growth(2014) PERES, Maria; OLIVEIRA, Ivone de; FURUKAWA, Luzia; GUIMARAES, Carina; HEIMANN, JoelconferenceObject Transgenerational Effects of High Salt Diet During Gestation(2016) PERES, Maria Angelica; OLIVEIRA, Ivone Braga de; FURUKAWA, Luzia Naoko Shinohara; HEIMANN, Joel- Exposure to fine particulate matter in the air alters placental structure and the renin-angiotensin system(2017) SOTO, Sonia de Fatima; MELO, Juliana Oliveira de; MARCHESI, Guilherme D'Aprile; LOPES, Karen Lucasechi; VERAS, Mariana Matera; OLIVEIRA, Ivone Braga de; SOUZA, Regiane Machado de; CASTRO, Isac de; FURUKAWA, Luzia Naoko Shinohara; SALDIVA, Paulo Hilario Nascimento; HEIMANN, Joel C.Transforming growth factor beta 1 (TGF beta 1), the uteroplacental renin-angiotensin system (RAS) and vascular endothelial growth factor A (VEGF-A) participate in the placentation process. The aim of this study was to investigate the effects of exposure to pollutants on the placenta. Methods Female Wistar rats were exposed to filtered air (F) or to concentrated fine particulate matter (P) for 15 days. After mating, the rats were divided into four groups and again exposed to F or P (FF, FP, PF, PP) beginning on day 6 of pregnancy. At embryonic day 19, the placenta was collected. The placental structure, the protein and gene expression of TGF beta 1, VEGF-A, and its receptor Flk-1 and RAS were evaluated by indirect ELISA and quantitative real-time PCR. Results Exposure to P decreased the placental mass, size, and surface area as well as the TGF beta 1, VEGF-A and Flk-1 content. In the maternal portion of the placenta, angiotensin II (AngII) and its receptors AT(1) (AT(1)R) and AT(2) (AT(2)R) were decreased in the PF and PP groups. In the fetal portion of the placenta, AngII in the FP, PF and PP groups and AT(2)R in the PF and PP groups were decreased, but AT(1)R was increased in the FP group. VEGF-A gene expression was lower in the PP group than in the FF group. Conclusions Exposure to pollutants before and/or during pregnancy alters some characteristics of the placenta, indicating a possible impairment of trophoblast invasion and placental angiogenesis with possible consequences for the maternal-fetal interaction, such as a limitation of fetal nutrition and growth.
conferenceObject Low salt intake during pregnancy alters glucose metabolism and DNA methylation in the offspring(2014) SIQUEIRA, F. R.; FURUKAWA, L. N. S.; OLIVEIRA, I. B.; HEIMANN, J. C.- Glucose metabolism and hepatic Igf1 DNA methylation are altered in the offspring of dams fed a low-salt diet during pregnancy(2016) SIQUEIRA, Flavia R.; FURUKAWA, Luzia N. S.; OLIVEIRA, Ivone B.; HEIMANN, Joel C.A low-salt (LS) diet during pregnancy has been linked to insulin resistance in adult offspring, at least in the experimental setting. However, it remains unclear if this effect is due to salt restriction during early or late pregnancy. To better understand this phenomenon, 12-week-old female Wistar rats were fed a LS or normal-salt (NS) diet during gestation or a LS diet during either the first (LS10) or second (LS20) half of gestation. Glucose tolerance test, HOMA-IR, gene expression analysis and DNA methylation measurements were conducted for the Insr, Igf1, Igf1r, Ins1 and Ins2 genes in the livers of neonates and in the liver, white adipose tissue and muscle of 20-week-old male offspring. Birth weight was lower in the IS20 and IS animals compared with the NS and LS10 rats. In the liver, the Igf1 levels in the LS10, LS20 and IS neonates were lower than those in the NS neonates. Methylation of the Insr, Igf1r, Ins1 and Ins2 genes was influenced in a variable manner by low salt intake during pregnancy. Increased liver Igf1 methylation was observed in the LS and LS20 neonates compared with their NS and LS10 counterparts. Glucose intolerance was observed in adult offspring as an effect of low salt intake over the duration of pregnancy. Compared to the NS animals, the HOMA-IR was higher in the 12-week-old IS and 20-week-old LS-10 rats. Based on these results, it appears that the reason a LS diet during pregnancy induces a low birth weight is its negative correlation with Igf1 DNA methylation in neonates.
- Isolated total RNA and protein are preserved after thawing for more than twenty-four hours(2012) OLIVEIRA, Ivone Braga de; RAMOS, Debora Rothstein; LOPES, Karen Lucasechi; SOUZA, Regiane Machado de; HEIMANN, Joel Claudio; FURUKAWA, Luzia Naoko ShinoharaOBJECTIVE: The preservation of biological samples at a low temperature is important for later biochemical and/or histological analyses. However, the molecular viability of thawed samples has not been studied sufficiently in depth. The present study was undertaken to evaluate the viability of intact tissues, tissue homogenates, and isolated total RNA after defrosting for more than twenty-four hours. METHODS: The molecular viability of the thawed samples (n = 82) was assessed using the A260/A280 ratio, the RNA concentration, the RNA integrity, the level of intact mRNA determined by reverse transcriptase polymerase chain reaction, the protein level determined by Western blotting, and an examination of the histological structure. RESULTS: The integrity of the total RNA was not preserved in the thawed intact tissue, but the RNA integrity and level of mRNA were perfectly preserved in isolated defrosted samples of total RNA. Additionally, the level of beta-actin protein was preserved in both thawed intact tissue and homogenates. CONCLUSION: Isolated total RNA does not undergo degradation due to thawing for at least 24 hours, and it is recommended to isolate the total RNA as soon as possible after tissue collection. Moreover, the protein level is preserved in defrosted tissues.
conferenceObject HIGH-SALT INTAKE DURING PREGNANCY MODIFIES PLACENTA PHENOTYPE AT LEAST UNTIL THE THIRD GENERATION(2014) PERES, Maria A.; SOTO, Sonia F.; GUIMARADES, Carina F.; SILVA, Rafaela C. P.; OLIVEIRA, Ivone B. de; FURUKAWA, Luzia N. S.; HEIMANN, Joel C.