VINICIUS CESTARI DO AMARAL
Projetos de Pesquisa
Unidades Organizacionais
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
13 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 13
bookPart Análogos do hormônio liberador de gonadotrofina(2014) JúNIOR, José Maria Soares; AMARAL, Vínicius Cestari do; BARACAT, Maria Cândida Pinheiro; SIMõES, Ricardo dos Santos; BARACAT, Edmund ChadaconferenceObject Cytoskeleton remodeling and migration of T47D breast cancer cell enhanced by prolactin(2016) SILVA, Priscilla Da; AMARAL, Vinicius Do; BARACAT, Edmund; SOARES- JR., Jose Maria; SIMONCINI, Tommaso- Differences in neonatal exposure to estradiol or testosterone on ovarian function and hormonal levels(2015) MARCONDES, Rodrigo R.; CARVALHO, Katia C.; DUARTE, Daniele C.; GARCIA, Natalia; AMARAL, Vinicius C.; SIMOES, Manuel J.; TURCO, Edson G. Lo; SOARES JR., Jose M.; BARACAT, Edmund C.; MACIEL, Gustavo A. R.Exposure to an excess of androgen or estrogen can induce changes in reproductive function in adult animals that resemble polycystic ovary syndrome in humans. However, considerable differences exist among several types of animal models. Little is known about the molecular features of steroidogenesis and folliculogenesis in the ovaries of rats exposed to different sex steroids as neonates. Here, we evaluated the impact of androgen and estrogen exposure on the ovaries of adult female rats during their neonatal period in the gene expression of Lhr and Cyp17a1, two key players of steroidogenesis. We also assessed hormone levels, folliculogenesis and the theca-interstitial cell population. The study was performed on the second postnatal day in thirty female Wistar rats that were sorted into the following three intervention groups: testosterone, estradiol and vehicle (control group). The animals were euthanized 90 days after birth. The main outcomes were hormone serum levels, ovary histomorphometry and gene expression of Lhr and Cyp17a1 as analyzed via quantitative real-time PCR. We found that exposure to excess testosterone in early life increased the LH and testosterone serum levels, the LH/FSH ratio, ovarian theca-interstitial area and gene expression of Lhr and Cyp17a1 in adult rats. Estrogen induced an increase in the ovarian theca-interstitial area, the secondary follicle population and gene expression of Lhr and Cyp17a1. All animals exposed to the sex steroids presented with closed vaginas. Our data suggest that testosterone resulted in more pronounced reproductive changes than did estrogen exposure. Our results might provide some insight into the role of different hormones on reproductive development and on the heterogeneity of clinical manifestations of conditions such as polycystic ovary syndrome.
bookPart Hiperprolactinemia induzida em animais(2014) AMARAL, Vínicius Cestari do; JúNIOR, José Maria Soares; GOMES, Regina Célia Teixeira; SIMõES, Manuel de Jesus; BARACAT, Edmund Chada- Metoclopramide-induced hyperprolactinemia effects on the pituitary and uterine prolactin receptor expression(2013) AMARAL, Vinicius C.; MACIEL, Gustavo A. R.; CARVALHO, Katia C.; MARCONDES, Rodrigo R.; SOARES JR., Jose Maria; BARACAT, Edmund C.In this work we have evaluated the gene expression profile of prolactin and prolactin receptor in the pituitary and the uterus of female mice with metoclopramide-induced hyperprolactinemia treated with estrogen and/or progesterone. For this purpose, 49 Swiss female mice were allocated to seven groups. Interventions: 50-day treatment with metoclopramide, progesterone and estrogen. Our results showed that in the pituitary, metoclopramide-induced hyperprolactinemia increased prolactin expression. In the castrated animals, progesterone, with or without estrogen, produced an increase in prolactin. Pituitary prolactin receptor and the estrogen and progesterone treatment were responsible for the rise in PRLR-S2. In the uterus, no differences in prolactin expression were found between the different study groups. PRLR-S1 had its expression reduced in all castrated animals as against the castrated group treated with vehicle. In the noncastrated animals, PRLR-52 rose in the metoclopramide-treated group, and, in the castrated animals, its expression diminished in all groups in relation to the vehicle-treated castrated controls. An increase in PRLR-S3 was found in the oophorectomized animals treated with a combination of estrogen and progesterone. PRLR-L rose in the oophorectomized animals treated with progesterone in isolation or in association with estrogen. These findings suggest that metoclopramide associated to progesterone or estrogen may determine an increase in pituitary prolactin and PRLR-S2 expression. The estrogen-progesterone may enhance the expression of PRLR-S3 and PRLR-L isoform of prolactin receptor.
- The progesterone and estrogen modify the uterine prolactin and prolactin receptor expression of hyperprolactinemic mice(2015) AMARAL, Vinicius Cestari do; CARVALHO, Katia Candido; MACIEL, Gustavo Arantes Rosa; SIMONCINI, Tommaso; SILVA, Priscilla Ludovico da; MARCONDES, Rodrigo Rodrigues; SOARES JR., Jose Maria; BARACAT, Edmund ChadaThe aim of this study was to evaluate the effects of metoclopramide-induced hyperprolactinemia on the prolactin (PRL) and PRL receptor's expression in the uterus of mice. For this purpose, 49 Swiss mice were divided into the following groups: GrSS (non-ovariectomized mice given vehicle); GrMET (non-ovariectomized mice treated with metoclopramide); OvSS (ovariectomized mice given vehicle); OvMET (ovariectomized mice treated with metoclopramide); OvMET+17 beta E (ovariectomized mice treated with metoclopramide and 17 beta estradiol); OvMET+MP (ovariectomized mice treated with metoclopramide and micronized progesterone); OvMET+17 beta E+MP (ovariectomized mice treated with metoclopramide and a solution of 17 beta estradiol and micronized progesterone). Immunohistochemical analyzes were evaluated semi-quantitatively. Our results showed that GrMET, OvMET+MP, and OvMET+17 beta E+MP presented strong PRL expression. OvMET and OvMET+17 beta E presented mild reaction, while GrSS and OvSS presented weak reaction. Concerning PRL receptor, OvMET+MP and OvMET+17 beta E+MP showed strong reaction; GrMET, OvSS, and OvMET+17 beta E showed mild reaction; and GrSS and OvMET showed weak reaction. These findings suggest that progesterone alone or in combination with estrogen may increase the expression of uterine PRL and PRL receptor.
conferenceObject Estrogen enhances glucose transporter 4 and insulin substrate 1 expressions in SGBS-adipocyte submitted to low and high glucose concentration(2016) AMARAL, Vinicius Do; SOARES- JR., Jose Maria; SILVA, Priscilla Da; BARACAT, Edmund; SIMONCINI, TommasobookPart Hormônio liberador de gonadotrofina(2014) CARVALHO, Kátia Candido; AMARAL, Vinícius Cestari do; BARACAT, Maria Cândida Pinheiro; JúNIOR, José Maria Soares; BARACAT, Edmund Chada- Effects of metoclopramide-induced hyperprolactinemia on the prolactin and prolactin receptor expression of murine adrenal(2015) AMARAL, Vinicius Cestari do; SILVA, Priscilla Ludovico da; CARVALHO, Katia Candido; SIMONCINI, Tommaso; MACIEL, Gustavo Arantes Rosa; SOARES- JR., Jose Maria; BARACAT, Edmund ChadaThe aim of this study was to evaluate the effects of metoclopramide-induced hyperprolactinemia on the prolactin (PRL) and prolactin receptor's (PRLR) expression in the adrenal. For this purpose, a total of 12 animals with intact ovaries were allocated to two groups: G1 (saline solution) and G2 (metoclopramide). A total of 30 oophorectomized animals was randomized to five subgroups: G3 (saline solution), G4 (metoclopramide), G5 (metoclopramide + 17 beta-estradiol), G6 (metoclopramide + progesterone), and G7 (metoclopramide + 17 beta-estradiol + progesterone). Immunohistochemical analyses were evaluated semi-quantitatively. For PRLR, the area fraction of labeled cells (ALC) varied from 1 (0-10%) to 3 (> 50%). Based on the mean of the immunostaining intensity, G2 and G4 showed strong expression; G6 and G7 presented a mild reaction; and G1, G3, and G5 exhibited a weak reaction. Concerning PRL, the ALC varied from 1 (0-10%) to 3 (> 50%), and groups G6 and G7 showed a strong reaction; G2, G4, and G5 showed a mild reaction; and G1 and G3 exhibited a weak reaction. These findings suggest that metoclopramide-induced hyperprolactinemia increases PRL expression in the adrenal glands of mice. Furthermore, progesterone alone or in association with estrogen also increases PRL expression, but to a lesser extent.
conferenceObject MORPHOLOGICAL ANALYSIS OF TRABECULAR BONE IN PERSISTENT ESTROUS RAT MODELS INDUCED BY SEXUAL STEROIDS: PRELIMINARY RESULTS(2012) MARCONDES, R. R.; CONDI, F.; DUARTE, D. C.; AMARAL, V. C.; PARRA, E.; BARACAT, E. C.; MACIEL, G. A. R.Aims: Our aim is to analyze how is the trabecular bone in persistent estrous rat models that mimic polycystic ovary syndrome. Methods: 15 animals received between 0-3 days of age only one subcutaneous injection of the following substances according with the experimental groups: testosterone propionate (1.25 mg/0.1 mL of vehicle) (testosterone group - TG; n 05), estradiol benzoate (0.5 mg/0.1 mL of vehicle) (estradiol group - EG; n 05), vehicle (0.1 mL) (control group - CG; n 05). Estrous cycle of each rat was assessed by the vaginal smears taken between 75-90 days old. The weight of the animals was done once a week between the birth and 84 days old. Animals were previously anaesthetized and sacrificed with 90 days old and ovary and femur were removed for histological routine. The histological sections were stained with hematoxylin-eosin for evaluating cysts in ovary and trabecular bone structure. ANOVA supplemented by Tukey’s multiple comparisons test was performed to evaluate the weight of the animals. Results: Animals of CG had normal estrous cycle, but EG and TG animals had closed vagina, and because ofthis vaginal smears were not collected. Ovarian histology revealed that EG and TG have cysts and did not have corpora lutea, which indicates that these animals were in chronic anovulation state (persistent estrous). CG ovaries showed corpora lutea as well follicles at different stages of development. Regarding the animals’ weight, EG exhibits the larger mean (320.1± 13.2 g), followed by TG (298.2±19.8 g) and after by CG (260.2±15.3 g). Statistical comparison between CG and EG weight showed significant difference (p<0.05) as well as the comparison between CG and TG (p<0.05). However, there was no significant difference between TG and EG. Evaluation of trabecular bone showed that control group presented great amount of bone trabeculae, as well as preserved thickness and connectivity. EG showed less trabecular connectivity, but also showed great amount of trabecular bone and preserved thickness. The amount of bone trabeculae and trabecular thickness and connectivity were increased in TG in relation to CG. Conclusion: We concluded that testosterone persistent estrous rat model presents improved trabecular bone quality and the changes induced by estradiol in perinatal period seems to be restricted to less trabecular connectivity, but we are doing the morphometric analysis to achieve a better conclusion.