VANESSA DOS SANTOS MORAIS
Projetos de Pesquisa
Unidades Organizacionais
LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina
7 resultados
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Agora exibindo 1 - 7 de 7
- Multiple clades of Husavirus in South America revealed by next generation sequencing(2021) RAMOS, Endrya do Socorro Foro; ROSA, Ulisses Alves; RIBEIRO, Geovani de Oliveira; VILLANOVA, Fabiola; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; MORAIS, Vanessa dos Santos; ARAUJO, Emerson Luiz Lima; PANDEY, Ramendra Pati; RAJ, V. Samuel; SABINO, Ester Cerdeira; DENG, Xutao; DELWART, Eric; LUCHS, Adriana; LEAL, Elcio; COSTA, Antonio Charlys daHusavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs' nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study.
- Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016(2021) LUCHS, Adriana; TARDY, Kaelan; TAHMASEBI, Roozbeh; MORILLO, Simone Guadagnucci; MILAGRES, Flavio Augusto de Padua; MORAIS, Vanessa dos Santos; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; AZEVEDO, Lais Sampaio de; SOUZA, Ellen Viana de; MEDEIROS, Roberta Salzone; SOUZA, Yasmin Franca Viana Pires de; ARAUJO, Emerson Luiz Lima; WITKIN, Steven S.; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; LEAL, Elcio; COSTA, Antonio Charlys daThis study combined conventional epidemiology of human astroviruses. From 2010 to 2016, 232 stool samples from children under 5 years of age were screened using NGS and conventional RT-PCR followed by genetic analysis in order to investigate the genotypic diversity of classical human astrovirus (HAstV) circulating in Tocantins State, Brazil. HAstV was detected in 16 cases (6.9%). Seven specimens (43.7%; 7/16) were positive according RT-PCR and next-generation sequencing (NGS) to investigate the molecular to both NGS and RT-PCR. NGS and RT-PCR individually revealed six (37.5%; 6/16) and three (18.8%; 3/16) additional positive samples, respectively. Sequencing of the HAstV-positive samples revealed HAstV-1a (9/16), HAstV-4c (3/16), and HAstV-5c (4/16) lineages.
- Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil(2021) TINKER, Rory J.; COSTA, Antonio Charlys da; TAHMASEBI, Roozbeh; MILAGRES, Flavio Augusto de Padua; MORAIS, Vanessa dos Santos; PANDEY, Ramendra Pati; JOSE-ABREGO, Alexis; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; CUNHA, Mariana Sequetin; ARAUJO, Emerson Luiz Lima; GOMEZ, Mariela Martinez; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; LEAL, Elcio; LUCHS, AdrianaFrom 2010-2016, a total of 251 stool samples were screened for norovirus using next-generation sequencing (NGS) followed by phylogenetic analysis to investigate the genotypic diversity of noroviruses in rural and low-income urban areas in northern Brazil. Norovirus infection was detected in 19.9% (50/251) of the samples. Eight different genotypes were identified: GII.4_Sydney[P31] (64%, 32/50), GII.6[P7] (14%, 7/50), GII.17[P17] (6%, 3/50), GII.1[P33] (6%, 3/50), GII.3[P16] (4%, 2/50), GII.2[P16] (2%, 1/50), GII.2[P2] (2%, 1/50), and GII.4_New Orleans[P4] (2%, 1/50). Distinct GII.6[P7] variants were recognized, indicating the presence of different co-circulating strains. Elucidating norovirus genetic diversity will improve our understanding of their potential health burden, in particular for the GII.4_Sydney[P31] variant.
- Guapiacu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil(2021) RIBEIRO, Geovani de Oliveira; COSTA, Antonio Charlys da; GILL, Danielle Elise; RIBEIRO, Edcelha Soares D'Athaide; REGO, Marlisson Octavio da S.; MONTEIRO, Fred Julio Costa; VILLANOVA, Fabiola; NOGUEIRA, Juliana Silva; MAEDA, Adriana Yurika; SOUZA, Renato Pereira de; TAHMASEBI, Roozbeh; MORAIS, Vanessa S.; PANDEY, Ramendra Pati; RAJ, V. Samuel; SCANDAR, Sirle Abdo Salloum; VASAMI, Fernanda Gisele da Silva; D'AGOSTINO, Leandro Guaraglia; MAIORKA, Paulo Cesar; DENG, Xutao; NOGUEIRA, Mauricio Lacerda; SABINO, Ester Cerdeira; DELWART, Eric; LEAL, Elcio; CUNHA, Mariana SequetinClassical insect-flaviviruses (cISFVs) and dual host-related insect-specific flavivirus (dISFV) are within the major group of insect-specific flavivirus. Remarkably dISFV are evolutionarily related to some of the pathogenic flavivirus, such as Zika and dengue viruses. The Evolutionary relatedness of dISFV to flavivirus allowed us to investigate the evolutionary principle of host adaptation. Additionally, dISFV can be used for the development of flavivirus vaccines and to explore underlying principles of mammalian pathogenicity. Here we describe the genetic characterization of a novel putative dISFV, termed Guapiacu virus (GUAPV). Distinct strains of GUAPV were isolated from pools of Aedes terrens and Aedes scapularis mosquitoes. Additionally, we also detected viral GUAPV RNA in a plasma sample of an individual febrile from the Amazon region (North of Brazil). Although GUAPV did not replicate in tested mammalian cells, 3 ' UTR secondary structures duplication and codon usage index were similar to pathogenic flavivirus.
- High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis(2021) RAMOS, Endrya do Socorro Foro; ROSA, Ulisses Alves; RIBEIRO, Geovani de Oliveira; VILLANOVA, Fabiola; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; MORAIS, Vanessa dos Santos; BERTANHE, Mayara; MARCATTI, Roberta; ARAUJO, Emerson Luiz Lima; WITKIN, Steven S.; DELWART, Eric; LUCHS, Adriana; COSTA, Antonio Charlys da; LEAL, ElcioEchoviruses (E) are a diverse group of viruses responsible for various pathological conditions in humans including aseptic meningitis, myocarditis, and acute flaccid paralysis. The detection and identification of echovirus genotypes in clinical samples is challenging due to its high genetic diversity. Here, we report the complete genome sequences of nine echoviruses, obtained by next-generation sequencing of 238 fecal samples from individuals with gastroenteritis in regions of Brazil. Detected viruses were classified into six genotypes: Three E1 sequences (BRA/TO-028, BRA/TO-069 and BRA/TO-236), one E3 (BRA/TO-018), one E11 (BRA/TO-086), one E20 (BRA/TO-016), two E29 (BRA/TO-030 and BRA/TO-193), and one E30 sequence (BRA/TO-032). Phylogenetic analysis indicated that the echoviruses E1 and E29 circulating in Brazil are divergent from strains circulating worldwide. The genotype diversity identified in our study may under-represent the total echovirus diversity in Brazil because of the small sample size and the restricted geographical distribution covered by the survey.
- Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon(2021) RIBEIRO, Geovani de Oliveira; GILL, Danielle Elise; RIBEIRO, Edcelha Soares D. Athaide; MONTEIRO, Fred Julio Costa; MORAIS, Vanessa S.; MARCATTI, Roberta; REGO, Marlisson Octavio da S.; ARAUJO, Emerson Luiz Lima; WITKIN, Steven S.; VILLANOVA, Fabiola; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; LEAL, Elcio; COSTA, Antonio Charlys daDengue virus (DENV) is a mosquito-borne viral pathogen that plagues many tropical-climate nations around the world, including Brazil. Molecular epidemiology is a growing and increasingly invaluable tool for understanding the dispersal, persistence, and diversity of this impactful virus. In this study, plasma samples (n = 824) from individuals with symptoms consistent with an arboviral febrile illness were analyzed to identity the molecular epidemiological dynamics of DENV circulating in the Brazilian state of Amapa. Twelve DENV type 1 (DENV-1) genomes were identified, which were phylogenetically related to the BR4 lineage of genotype V. Phylodynamics analysis suggested that DENV-1 BR-4 was introduced into Amapa around early 2010, possibly from other states in northern Brazil. We also found unique amino acids substitutions in the DENV-1 envelope and NS5 protein sequences in the Amapa isolates. Characterization of the DENV-1 BR-4 sequences highlights the potential of this new lineage to drive outbreaks of dengue in the Amazon region.
- New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples(2021) VILLANOVA, Fabiola; MARCATTI, Roberta; BERTANHE, Mayara; MORAIS, Vanessa dos Santos; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; ARAUJO, Emerson Luiz Lima; TAHMASEBI, Roozbeh; WITKIN, Steven S.; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; ABREU-JUNIOR, Cassio Hamilton; LEAL, Elcio; COSTA, Antonio Charlys daSquash mosaic virus (SqMV) is a phytovirus that infects great diversity of plants worldwide. In Brazil, the SqMV has been identified in the states of Ceara, Maranhao, Piaui, Rio Grande do Norte, and Tocantins. The presence of non-pathogenic viruses in animals, such as phytoviruses, may not be completely risk-free. Similarities in gene repertories between these viruses and viruses that affect animal species have been reported. The present study describes the fully sequenced genomes of SqMV found in human feces, collected in Tocantins, and analyzes the viral profile by metagenomics in the context of diarrhea symptomatology. The complete SqMV genome was obtained in 39 of 253 analyzed samples (15.5%); 97.4% of them belonged to children under 5 years old. There was no evidence that the observed symptoms were related to the presence of SqMV. Of the different virus species detected in these fecal samples, at least 4 (rotavirus, sapovirus, norovirus, parechovirus) are widely known to cause gastrointestinal symptoms. The presence of SqMV nucleic acid in fecal samples is likely due to recent dietary consumption and it is not evidence of viral replication in the human intestinal cells. Identifying the presence of SqMV in human feces and characterization of its genome is a relevant precursor to determining whether and how plant viruses interact with host cells or microorganisms in the human gastrointestinal tract.