CAMILA CANTEIRO LEANCA
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- Metabolism of plasma cholesterol and lipoprotein parameters are related to a higher degree of insulin sensitivity in high HDL-C healthy normal weight subjects(2013) LEANCA, Camila C.; NUNES, Valeria S.; PANZOLDO, Natalia B.; ZAGO, Vanessa S.; PARRA, Eliane S.; CAZITA, Patricia M.; JAUHIAINEN, Matti; PASSARELLI, Marisa; NAKANDAKARE, Edna R.; FARIA, Eliana C. de; QUINTAO, Eder C. R.Background: We have searched if plasma high density lipoprotein-cholesterol (HDL-C) concentration interferes simultaneously with whole-body cholesterol metabolism and insulin sensitivity in normal weight healthy adult subjects. Methods: We have measured the activities of several plasma components that are critically influenced by insulin and that control lipoprotein metabolism in subjects with low and high HDL-C concentrations. These parameters included cholesteryl ester transfer protein (CETP), phospholipid transfer protein (PLTP), lecithin cholesterol acyl transferase (LCAT), post-heparin lipoprotein lipase (LPL), hepatic lipase (HL), pre-beta-1HDL, and plasma sterol markers of cholesterol synthesis and intestinal absorption. Results: In the high-HDL-C group, we found lower plasma concentrations of triglycerides, alanine aminotransferase, insulin, HOMA-IR index, activities of LCAT and HL compared with the low HDL-C group; additionally, we found higher activity of LPL and pre-beta-1HDL concentration in the high-HDL-C group. There were no differences in the plasma CETP and PLTP activities. Conclusions: These findings indicate that in healthy hyperalphalipoproteinemia subjects, several parameters that control the metabolism of plasma cholesterol and lipoproteins are related to a higher degree of insulin sensitivity.
conferenceObject Plasma 27-hydroxycholesterol/cholesterol Ratio is Increased in Low High Density Lipoprotein-Cholesterol Healthy Subjects(2012) NUNES, Valeria S.; LEANCA, Camila C.; PANZOLDO, Natalia B.; PARRA, Eliane; ZAGO, Vanessa; CAZITA, Patricia M.; NAKANDAKARE, Edna R.; FARIA, Eliana C. de; QUINTAO, Eder C.- Plasma 27-hydroxycholesterol/cholesterol ratio is increased in low high density lipoprotein-cholesterol healthy subjects(2013) NUNES, Valeria S.; LEANCA, Camila C.; PANZOLDO, Natalia B.; PARRA, Eliane; ZAGO, Vanessa; CAZITA, Patricia M.; NAKANDAKARE, Edna R.; FARIA, Eliana C. de; QUINTAO, Eder C. R.Sterol 27-hydroxylase converts cholesterol to 27-hydroxycholesterol (27-OHC) which is widely distributed among tissues and is expressed at high levels in the vascular endothelium and macrophages. There is a continuous flow of this oxysterol from the tissues into the liver; where it is converted to bile acids. Objective: Measure plasma concentrations of 27-OHC in subjects that differ according to their plasma HDL-C concentration. Methods: Healthy men presenting low HDL-C (<1,03 mmol/L), n = 18 or high HDL-C (>1.55 mmol/L), n 18, BMI <30 kg/m(2) were recruited after excluding secondary causes that might interfere with their plasma lipid concentrations such as smoking, heavy drinking and diabetes. Blood samples were drawn after a 12 h fasting period for the measurement of 27-OHC by the combined GC/MS analysis utilizing deuterium-label internal standards. Results: The plasma ratio 27-OHC/total cholesterol (median and range nmoL/mmoL) was 50.41 (27.47-116.00) in the High HDL-C subjects and 6334 (36.46-91.18) in the Low HDL-C subjects (p = 0.0258). Conclusion: Our data indicate that the production of 27-0HC by extrahepatic tissues and its transport to the liver may represent an alternative pathway for a deficient reverse cholesterol transport system when plasma HDL-C is low.
- Increased 27-hydroxycholesterol plasma level in men with low high density lipoprotein-cholesterol may circumvent their reduced cell cholesterol efflux rate(2014) NUNES, Valeria S.; PANZOLDO, Natalia B.; LEANCA, Camila C.; PARRA, Eliane S.; ZAGO, Vanessa S.; SILVA, Eliton J. da; CAZITA, Patricia M.; NAKANDAKARE, Edna R.; FARIA, Eliana C. de; QUINTAO, Eder C. R.Background: HDL is considered the most important mechanism for the excretion of intracellular cholesterol. The liver is the only organ capable to metabolize cholesterol into bile acid. The enzymatic conversion of cholesterol to bile acid is dependent on the cytochrome P450 microsomal system which is also responsible for the generation of oxysterols. The latter's plasma concentrations may reflect the metabolic processes of specific tissues where they are generated. The objective of this study was to investigate in healthy individuals who differ according to their HDL levels the concentration of oxysterols and relate it to the HDL-dependent cell cholesterol efflux rate. Methods: 24-Hydroxycholesterol, 25-hydroxycholesterol, 27-hydroxycholesterol were determined in plasma by GLC/mass spectrometry in 107 healthy subjects with low HDL (HDL-C < 1.03 mmol/l) and high HDL cholesterol (HDL-C > 1.55 mmol/l). HDL-dependent in vitro cell cholesterol efflux rate was measured in 29 cases. Results: No differences were found in plasma oxysterol concentrations between the Low HDL and High HDL groups. There was a significant negative correlation between HDL-C and 27-hydroxycholesterol. Plasma oxysterol concentrations were significantly lower in female than in male subjects. The Low HDL male group had higher 27-hydroxycholesterol than the High HDL male group. Cell cholesterol efflux rate was lower in Low HDL than in High HDL and related inversely with 27-hydroxycholesterol. Conclusion: As compared to High HDL, Low HDL men have increased 27-hydroxycholesterol plasma level that may circumvent their reduced cell cholesterol efflux rate.
- Does plasma HDL-C concentration interact with whole-body cholesterol metabolism?(2013) LEANCA, C. C.; NUNES, V. S.; NAKANDAKARE, E. R.; FARIA, E. C. de; QUINTAO, E. C. R.This review examines the interactions between plasma high density lipoprotein (HDL) metabolism and whole-body cholesterol economy. More specifically, this review addresses three questions: 1) does plasma HDL-C concentration correlate with the parameters of whole-body cholesterol metabolism? 2) Do variations in cholesterol metabolism interfere with plasma HDL-C concentrations? 3) Are the markers of cholesterol synthesis and intestinal absorption specifically under the control of plasma HDL? The following answers were provided to each question, respectively: 1) plasma HDL influences whole-body cholesterol synthesis rate but the evidence that HDL modifies the total amount of cholesterol absorbed by the intestine is not clearly supported by present investigations; 2) there are suggestions that changes in whole body cholesterol metabolism rates do not interfere with plasma HDL-C concentrations; 3) markers of cholesterol synthesis and absorption may specifically be controlled by plasma HDL-C concentrations regarding the genetic causes of extremely low HDL-C concentrations, although within the general population plasma HDL-C concentration is likely ascribed to insulin resistance or diabetes mellitus.
- HDL-C concentration is related to markers of absorption and of cholesterol synthesis: Study in subjects with low vs. high HDL-C(2011) NUNES, V. S.; LEANCA, C. C.; PANZOLDO, N. B.; PARRA, E.; CAZITA, P. M.; NAKANDAKARE, E. R.; FARIA, E. C. de; QUINTAO, E. C. R.Background: The antiatherogenic functions of high density lipoprotein (HDL-C) include its role in reverse cholesterol transport, but to what extent the concentration of HDL-C interferes with the whole-body cholesterol metabolism is unknown. Therefore, we measured markers of body cholesterol synthesis (desmosterol and lathosterol) and of intestinal cholesterol absorption (campesterol and beta-sitosterol) in healthy subjects that differ according to their plasma HDL-C concentrations. Methods: Healthy participants presented either low HDL-C (<40 mg/dl, n = 33,17 male and 16 female) or high HDL-C (>60 mg/dl, n = 33, 17 male and 16 female), BMI <30 kg/m(2), were paired according to age and gender, without secondary factors that might interfere with their plasma lipid concentrations. Plasma concentrations of non-cholesterol sterols were measured by the combined GC-MS analysis. Results: Plasma desmosterol did not differ between the two groups; however, as compared with the high HDL-C participants, the low HDL-C participants presented higher concentration of lathosterol and lower concentration of the intestinal cholesterol absorption markers campesterol and beta-sitosterol. Conclusion: Plasma concentrations of HDL, and not the activities of LCAT and CETP that regulate the reverse cholesterol transport system, correlate with plasma sterol markers of intestinal cholesterol absorption directly, and of cholesterol synthesis reciprocally.
- Development of a clinical laboratory data base of hyper and hypo alpha lipoproteins in Campinas-SP and neighboring region(2013) PARRA, Eliane Soler; ZAGO, Vanessa Helena de Souza; PANZOLDO, Natália Baratella; ALEXANDRE, Fernanda; VENDRAME, Felipe; VIRGINIO, Vitor Wilson de Moura; VIEIRA, Isabela Calanca; RODRIGUES, Marcelo Scarpari Dutra; CASSOL, Angela de Souza; D'ALEXANDRI, Fabio; CASTANHO, Vera Sylvia; NAKAMURA, Rui; BARACAT, Jamal; DANELON, Mirian Regina Gardin; POZETI, Ronise Carla Sass; FACIN, Ana Cândida Coutinho; HONORIO, Hilda Maria da Silva; MESQUITA, Simone de Cássia Dias; SILVA, Marcio Jorge da; LOT, Marielza Crosgnac Feres; NUNES, Valéria Sutti; LEANÇA, Camila Canteiro; NAKANDAKARE, Edna Regina; QUINTÃO, Eder Carlos Rocha; FARIA, Eliana Cotta deINTRODUCTION: The development of research for diagnosis, prevention and treatment of atherosclerotic cardiovascular disease is of utmost importance due to the fact that it is the main cause of morbidity and mortality in Brazil. OBJECTIVE: To demonstrate the phases of the selection process for candidates with the aim to develop a clinical-laboratorial database of hyper alpha lipoproteinemic patients (hyper A) - high density lipoprotein cholesterol (HDL-C) ≥ 68 mg/dl) and hypo alpha lipoproteinemic patients (hypo A) - HDL-C < 39 mg/dl. MATERIAL AND METHODS: The volunteers were contacted after selection of lipid profiles from individuals treated at the Sistema Único de Saúde (SUS), Campinas-SP and neighboring area. Afterwards, the selected patients went through blood collection, clinical examinations and answered questionnaires on dietary frequency and physical activity. After this preliminary evaluation, some individuals were convened to another blood collection and, subsequently, were submitted to an ultrasonographic exam of the carotid arteries. RESULTS: Only 0.6% and 0.3% from 598,288 lipid profiles were selected for hyper A and hypo A groups, respectively, including gender disparity. Lack of effective questionnaires (75%), missing calls (60%) and non-inclusion were the major hindrances in the construction of this database. DISCUSSION: The difficulties to obtain eligible candidates were also due to the low prevalence of both groups hypo A and hyper A and the high prevalence of pathologies that contribute to non-genetic variations of HDL-C. CONCLUSION: In spite of the obstacles in the development of this database, this study brought about several scientific publications. Furthermore, the development of molecular analyzes and functionality will shortly generate other findings, contributing to the diagnosis and follow-up of HDL dyslipidemias.