VIRGINIA SPINOLA QUINTAL
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Pediatria, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
bookPart Icterícia neonatal(2022) QUINTAL, Virginia Spinola; DURANTE, Patricia Prado- Dielectric Properties of Infant Formulae, Human Milk and Whole and Low-Fat Cow Milk Relevant for Microwave Heating(2019) LEITE, Juliana A. S.; QUINTAL, Virginia S.; TADINI, Carmen C.The dielectric properties of three different reconstituted infant formulae, human milk, as well as, whole and low-fat pasteurized cow milk were measured at temperature interval from (5 to 70)degrees C and at frequency of 2450 MHz. The dependency of the dielectric properties with temperature is presented. The values of penetration depth were determined and can be used to estimate the ideal bottle-feeding or container diameter to use in a domestic microwave oven or specific microwave equipment to pasteurize human milk in Human Milk Banks (HMB).
- Leite de mulheres brasileiras apresenta anticorpos IgA secretores (SIgA) que neutralizam o rotavírus G9P[5](2013) SANTOS, Simone M. R.; FERREIRA, Thalita L.; QUINTAL, Virginia S.; CARBONARE, Solange B.; TINO-DE-FRANCO, MileneObjective: To verify the presence of anti-rotavirus serotype G9P[5] SIgA and the virus neutralization capacity of milk samples from Brazilian women. Methods: SIgA antibody levels reactive to rotavirus G9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (ELISA) using purified virus suspensions. The samples' capacity to neutralize rotavirus G9P[5] was analyzed using the MA-104 cells neutralization assay. Results: Great individual variations were observed regarding the SIgA levels and neutralization titers, but all samples showed some G9P[5] neutralizing ability. A highly significant positive correlation was observed between antibody levels and neutralization titers. Conclusions: The high correlation between anti-rotavirus antibody levels and neutralizing capacity of the milk samples suggests a possible protective role of these antibodies against infection. These results also support the encouragement of the breast-feeding practice.
- Neutralizing activity and secretory IgA antibodies reactive with rotavirus SA-11 (serotype G3) in colostrum and milk from Brazilian women(2013) FRANCO, M. Tino De; VIEIRA, P. D.; SANTOS, S. M. R.; FERREIRA, T. L.; ARAUJO, E. D.; QUINTAL, V. S.; CARBONARE, S. B.Background: Rotavirus is an important aetiological agent for severe diarrhoea in infants and young children worldwide. Anti-rotavirus antibodies in human colostrum and milk may interfere with rotavirus vaccination seroconversion. Aims: To verify the presence of anti-rotavirus secretory IgA antibodies (SIgA) and the neutralizing capacity of 30 colostrum and 30 milk samples from Brazilian women in two different centres and analyze their persistence throughout lactation. Methods: Colostrum and milk samples from healthy nursing mothers were tested for the presence of anti-rotavirus SIgA using conventional ELISA and their capacity to neutralize rotavirus using MA-104 cell cultures. Total IgA concentrations and anti-rotavirus SIgA levels were measured in samples collected from three mothers during 90 or 240 days of the lactation period. Results: Colostrum samples showed higher levels of anti-rotavirus SIgA and higher neutralizing ability than in milk. However, these antibodies levels were not statistically different. In addition, there was no correlation between antibody levels and the neutralizing activity observed in colostrum and milk samples. Follow-up of three mothers demonstrated the persistence of anti-rotavirus and total IgA levels throughout lactation. Conclusions: These results support the encouragement of breastfeeding as a mechanism of protection against rotavirus infection in lactating infants. Components other than SIgA antibodies might play an important role in virus neutralization.
- Pasteurization efficiency of donor human milk processed by microwave heating(2019) LEITE, Juliana A. S.; MIGOTTO, Aurea M. A.; LANDGRAF, Mariza; QUINTAL, Virginia S.; GUT, Jorge A. W.; TADINI, Carmen C.The main objective of this work was to verify the efficiency of batch microwave-assisted heating for donor human milk and assess the activity of alkaline phosphatase (ALP) and microbiological quality. Processing was conducted at different temperatures between (50 and 80) degrees C and times from (5-300) s in order to evaluate the suitability of ALP as a target for the microwave process of donor human milk. The thermal inactivation of ALP followed first-order kinetics with a z-value of 4.4 degrees C that indicates a strong dependency of ALP inactivation with temperature. The optimal conditions (60 degrees C for 30 s) were determined, based on ALP inactivation, and the microbiological quality of the processed donor human milk was evaluated. No pathogens (mesophilic bacteria, coliforms at 35 degrees C, Salmonella spp. and Staphylococcus spp.) were detected after microwave-assisted heating and this suggests that this technology can be applied to ensure adequate safety and quality in Human Milk Banks (HMBs).