MARCELA DE SOUZA BASQUEIRA

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LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina

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Assunto: Chagas disease ×

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  • article 1 Citação(ões) na Scopus
    Lack of evidence of seronegative infection in an endemic area of Chagas disease
    (2019) OLIVEIRA, Lea Campos de; LEE, Tzong-Hae; FERREIRA, Ariela Mota; BIERRENBACH, Ana Luiza; SOUZA-BASQUEIRA, Marcela de; OLIVEIRA, Claudia Di Lorenzo; CARDOSO, Clareci Silva; MOREIRA, Carlos Henrique Valente; OIKAWA, Marcio K.; RIBEIRO, Antonio Luiz P.; BUSCH, Michael P.; SABINO, Ester Cerdeira
    The diagnosis of Chagas disease is based on the detection of Trypanosoma cruzi (T. cruzi)-specific antibodies. Nonetheless, there is concern about the sensitivity of current serological assays due to reports of T. cruzi PCR positivity among seronegative individuals. The aim of this study was to evaluate if T. cruzi seronegative infections occur in endemic areas. We recruited 2,157 individuals that were identified as having Chagas disease in a public health system database of an endemic region in Brazil. All participants were interviewed and 2,091 had a sample collected for serological and PCR testing. From these, 149 (7.1%) had negative serological results. PCR was positive in 610 samples (31.4%) of the 1,942 seropositive samples but in none of the 149 samples from seronegative participants. True T. cruzi seronegative infections seem to be rare (95% CI 0-3.7) and should not be a concern for blood supply, which relies on antibody screening.
  • article 12 Citação(ões) na Scopus
    Gut Dysbiosis in Chagas Disease. A Possible Link to the Pathogenesis
    (2020) SOUZA-BASQUEIRA, Marcela de; RIBEIRO, Roberto Marques; OLIVEIRA, Lea Campos de; MOREIRA, Carlos Henrique Valente; MARTINS, Roberta Cristina Ruedas; FRANCO, Diego Castillo; AMADO, Pamela Pontes Penas; MAYER, Marcia Pinto Alves; SABINO, Ester Cerdeira
    Chagas disease is caused by the flagellate protozoanTrypanosoma cruzi. Cardiomyopathy and damage to gastrointestinal tissue are the main disease manifestations. There are data suggesting that the immune response toT. cruzidepends on the intestinal microbiota. We hypothesized that Chagas disease is associated with an altered gut microbiome and that these changes are related to the disease phenotype. The stool microbiome from 104 individuals, 73 with Chagas disease (30 with the cardiac, 11 with the digestive, and 32 with the indeterminate form), and 31 healthy controls was characterized using 16S rRNA amplification and sequencing. The QIIME (Quantitative Insights Into Microbial Ecology) platform was used to analyze the data. Alpha and beta diversity indexes did not indicate differences between the groups. However, the relative abundance ofVerrucomicrobia, represented primarily by the genusAkkermansia, was significantly lower in the Chagas disease groups, especially the cardiac group, compared to the controls. Furthermore, differences in the relative abundances ofAlistipes, Bilophila, andDialisterwere observed between the groups. We conclude thatT. cruziinfection results in changes in the gut microbiome that may play a role in the myocardial and intestinal inflammation seen in Chagas disease.