MICHELE TATIANA PEREIRA TOMITAO
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
13 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 13
conferenceObject Polymorphisms in the cyclooxygenase-2 (COX-2) gene, vascular endothelial growth factor (VEGF) and methylenetetrahydrofolate reductase (MTHFR) gene in patients with hepatocellular carcinoma and hepatitis C virus infection(2017) CARVALHO, S. C. R. D.; VASCONCELOS, L. R. S.; CARMO, R. F.; AROUCHA, D. C. B. L.; TOMITAO, M. T.; RIBEIRO JR., U.; PEREIRA, L. M. M. B.; OLIVEIRA, C. P.; CARRILHO, F. J.conferenceObject ECONOMIC ANALYSIS OF HEPATO CELLULAR CARCINOMA TREATMENT BY SEVERITY OF DISEASE(2017) TANI, J. A.; CAMPOLINA, A. G.; DECIMONI, T. C.; TOMITAO, M.; FERREIRA, L. A.conferenceObject Wild Homozygous VEGF-A and COX-2 Gene Polymorphisms Are Associated to Worst Prognosis in Patients With Colorectal Cancer (CRC)(2013) TOMITAO, Michele T.; COTTI, Guilherme C.; KUBRUSLY, Marcia S.; PELEGRINELLI-ZAIDAN, Evelise; SAFATLE-RIBEIRO, Adriana V.; PATZINA, Rosely A.; ELUF-NETO, Jose; CECCONELLO, Ivan; NAHAS, Sergio C.; RIBEIRO, UlyssesconferenceObject RESOURCE UTILIZATION AND TREATMENT OF PATIENT WITH HEPATOCELULLAR CARCINOMA - A MICROCOSTING ANALYSIS(2017) TANI, J. A.; DECIMONI, T. C.; FERREIRA, L. A.; TOMITAO, M.; CAMPOLINA, A. G.- Carnoy's solution is an adequate tissue fixative for routine surgical pathology, preserving cell morphology and molecular integrity(2015) PEREIRA, Marina A.; DIAS, Andre R.; FARAJ, Sheila F.; CIRQUEIRA, Cinthya dos S.; TOMITAO, Michele T.; NAHAS, Sergio Carlos; RIBEIRO JR., Ulysses; MELLO, Evandro S. deAims: To compare Carnoy's solution (CS) and 10% neutral buffered formalin solution (NBF) as tissue fixatives in colorectal cancer specimens. Methods and results: Surgical specimens from patients with colorectal cancer were analysed. Three groups were studied, as follows: group 1 consisted of 16 paired samples fixed in CS and NBF; and groups 2 and 3 consisted of 14 prospective and 80 retrospective samples, respectively, both randomized for fixation in CS or NBF. Groups 1 and 2 were analysed for amount, quality and integrity of DNA. Morphological analysis, including some of the usual special stains and polymerase chain reaction (PCR), were also performed for group 1, and Sanger sequencing for group 2. Immunohistochemical (IHC) reactions for mismatch repair proteins were studied in groups 1 and 3. Fixative performances were similar for morphology, special stains, and IHC reactions, as well as for the amount, quality and integrity of extracted DNA. PCR amplification was not possible in two cases from CS group 1. Sanger sequencing gave conclusive results for the CS samples tested. Conclusions: Carnoy's solution and NBF are equivalent fixatives for colorectal cancer specimens and are adequate for routine utilization in surgical and molecular pathology.
- Risk factors for advanced duodenal and ampullary adenomatosis in familial adenomatous polyposis: a prospective, single-center study(2018) SULBARAN, M.; CAMPOS, F. G.; RIBEIRO JR., U.; KISHI, H. S.; SAKAI, P.; MOURA, E. G. H. de; BUSTAMANTE-LOPEZ, L.; TOMITAO, M.; NAHAS, S. C.; CECCONELLO, I.; SAFATLE-RIBEIRO, A. V.Background and study aims To determine the clinical features associated with advanced duodenal and ampullary adenomas in familial adenomatous polyposis. Secondarily, we describe the prevalence and clinical significance of jejunal polyposis. Patients and methods This is a single center, prospective study of 62 patients with familial adenomatous polyposis. Duodenal polyposis was classified according to Spigelman and ampullary adenomas were identified. Patients with Spigelman III and IV duodenal polyposis underwent balloon assisted enteroscopy. Predefined groups according to Spigelman and presence or not of ampullary adenomas were related to the clinical variables: gender, age, family history of familial adenomatous polyposis, type of colorectal surgery, and type of colorectal polyposis. Results Advanced duodenal polyposis was present in 13 patients (21%; 9 male) at a mean age of 37.61 +/- 13.9 years. There was a statistically significant association between family history of the disease and groups according to Spigelman (P=0.03). Seven unrelated patients (6 male) presented ampullary adenomas at a mean age of 36.14 +/- 14.2 years. The association between ampullary adenomas and extraintestinal manifestations was statistically significant in multivariate analysis (P=0.009). Five endoscopic types of non-ampullary adenoma were identified, showing that lesions larger than 10mm or with a central depression presented foci of high grade dysplasia. Among 28 patients in 12 different families, a similar Spigelman score was identified; 10/12 patients (83.3%) who underwent enteroscopy presented small tubular adenomas with low grade dysplasia in the proximal jejunum. Conclusions Advanced duodenal polyposis phenotype may be predictable from disease severity in a first-degree relative. Ampullary adenomas were independently associated with the presence of extraintestinal manifestations.
- Association between Polymorphisms in Inflammatory Response-Related Genes and the Susceptibility, Progression and Prognosis of the Diffuse Histological Subtype of Gastric Cancer(2018) FURUYA, Tatiane K.; JACOB, Carlos E.; TOMITAO, Michele T. P.; CAMACHO, Lizeth C. C.; RAMOS, Marcus F. K. P.; ELUF-NETO, Jose; ALVES, Venancio A. F.; ZILBERSTEIN, Bruno; CECCONELLO, Ivan; RIBEIRO JR., Ulysses; CHAMMAS, RogerThe chronic inflammatory microenvironment and immune cell dysfunction have been described as critical components for gastric tumor initiation and progression. The diffuse subtype is related to poor clinical outcomes, pronounced inflammation, and the worst prognosis. We investigated the association of polymorphisms in inflammatory response-related genes (COX-2, OGG1, TNFB, TNFA, HSPA1L, HSPA1B, VEGFA, IL17F, LGALS3, PHB, and TP53) with gastric cancer susceptibility, progression and prognosis in a Brazilian sample, focusing on the diffuse subtype. We also performed the analysis regarding the total sample of cases (not stratified for tumor subtypes), allowing the comparison between the findings. We further investigated the polymorphisms in linkage disequilibrium and performed haplotype association analyses. In the case-control study, rs1042522 (TP53) was associated with a stronger risk for developing gastric cancer in the sample stratified for diffuse subtype patients when compared to the risk observed for the total cases; CTC haplotype (rs699947 / rs833061 / rs2010963 VEGFA) was associated with risk while rs699947 was associated with protection for gastric malignancy in the total sample. Regarding the associations with the clinicopathological features of gastric cancer, for the diffuse subtype we found that rs699947 and rs833061 (VEGFA) were associated with outcomes related to a worse progression while rs5275 (COX-2), rs909253 (TNFB), and rs2227956 (HSPA1L) were associated to a better progression of the disease. In the total sample, rs699947 and rs833061 (VEGFA), rs4644 (LGALS3), and rs1042522 (TP53) were able to predict a worse progression while rs5275 (COX-2), rs2227956 (HSPA1L), and rs3025039 (VEGFA) a better progression. Besides, rs909253 (TNFB) predicted protection for the overall and disease-free survivals for gastric cancer. In conclusion, these results helped us to clarify the potential role of these polymorphisms in genes involved in the modulation of the inflammatory response in the pathogenesis of gastric cancer.
- Cyclooxygenase-2 gene polymorphisms and susceptibility to colorectal cancer in a Brazilian population(2017) TOMITAO, Michele Tatiana Pereira; NAHAS, Sergio Carlos; KUBRUSLY, Marcia Saldanha; FURUYA, Tatiane Katsue; DINIZ, Marcio Augusto; MARIE, Suely Kazue Nagahashi; SAFATLE-RIBEIRO, Adriana Vaz; ELUF-NETO, Jose; CECCONELLO, Ivan; RIBEIRO JR., UlyssesBackground: Multi-ethnicity of Brazilian population displays high levels of genomic diversity. Polymorphism may detect people at higher risk of developing cancer, distinctive response to treatment, and prognosis. Cyclooxygenase-2 (COX-2) is induced in response to growth factors and cytokines, and is expressed in inflammatory diseases, precancerous lesions and colorectal cancer (CRC). The aim of this study was to evaluate the influence of COX-2 -1195A > G and 8473T > C polymorphisms as a risk factor of developing CRC. Methods: We evaluated COX-2 Single Nucleotide Polymorphism (SNP) of 230 CRC patients and 196 healthy controls by Real-Time Polymerase Chain Reaction. Results: Populations were in Hardy-Weinberg equilibrium (HWE), except for control group of 8473T > C SNP. The frequencies were similar in both groups for genotypes and haplotypes. There was no association between studied polymorphisms and risk of CRC. Conclusions: The gene polymorphisms studied do not participate in the genetic susceptibility to CRC in a Brazilian population.
conferenceObject Association between polymorphisms in inflammatory response related-genes and the susceptibility, progression, and prognosis of gastric cancer.(2018) FURUYA, Tatiane K.; JACOB, Carlos E.; TOMITAO, Michele T.; CORDOBA-CAMACHO, Lizeth C.; RAMOS, Marcus K.; ELUF-NETO, Jose; ALVES, Venancio A.; ZILBERSTEIN, Bruno; CECCONELLO, Ivan; RIBEIRO-JUNIOR, Ulysses; CHAMMAS, Roger- VEGF-A and Cox-2 gene polymorphisms and susceptibility to colorectal cancer in Brazilian population(2013) TOMITAO, Michele T. P.; KUBRUSLY, Marcia S.; COTTI, Guilherme C.; ZAIDAN, Evelise P.; SAFATLE-RIBEIRO, Adriana V.; MARIE, Suely K. N.; CECCONELLO, Ivan; NAHAS, Sergio C.; RIBEIRO, Ulysses