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(Fonte: Lattes)
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Departamento de Patologia, Faculdade de Medicina
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina

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Agora exibindo 1 - 4 de 4
  • article 3 Citação(ões) na Scopus
    Visceral leishmaniasis caused by Leishmania (Leishmania) amazonensis associated with Hodgkin's lymphoma
    (2022) PORTO, Victor Bertolo Gomes; CARVALHO, Laina Bubach; BUZO, Bruno Fernando; LITVOC, Marcelo Nobrega; SANTOS, Ana Catharina S.; ROCCI, Rafael Avila; SOARES, Sandra Regina Castro; ZAMPIERI, Ricardo Andrade; DUARTE, Maria Irma Seixas; LINDOSO, Jose Angelo Lauletta
    Visceral leishmaniasis (VL) is mainly caused by Leishmania (Leishmania) donovani and Leishmania (L.) infantum; however, other Leishmania species have been associated with VL. We report a case of a patient simultaneously diagnosed with VL caused by Leishmania (L.) amazonensis and Hodgkin's lymphoma. After treatment with liposomal amphotericin B and chemotherapy, the patient presented a clinical cure. This case report reinforces the hypothesis that other Leishmania species can cause visceral lesions mainly related to immunosuppression.
  • article 7 Citação(ões) na Scopus
    Nitro-Heterocyclic compounds induce apoptosis-like effects in Leishmania (L). amazonensis promastigotes
    (2019) MENDONCA, Daiane Barros Dias; SILVA, Renata Ellen Costa; PALACE-BERL, Fanny; TAKAKURA, Cleusa F. H.; SOARES, Sandra Regina C.; BRAZ, Lucia Maria Almeida; TAVARES, Leoberto Costa; LINDOSO, Jose Angelo Lauletta
    Background: Three drugs - pentavalent antimonials, amphotericin B and pentamidine - are currently used for leishmaniasis treatment. They are administered for long periods, only parenterally, and have high cardiac, renal and hepatic toxicities. Therefore, the investigation of new compounds is required. Nitro-heterocyclic derivatives have been used as possible drug candidates to treat diseases caused by trypanosomatids. Methods: Leishmania (L.) amazonensis promastigotes (MHO/BR/73/M2269), maintained in the Laboratorio de Soroepidemiologia - Instituto de Medicina Tropical-USP, were exposed to five nitroheterocyclic derivatives, with differences at phenyl-ring position 4: BSF-C4H9, BSF-H, BSF-NO2, BSF-CH3 and BSF-Cl, for 48 hours. After analyzing viability (MTT assay), we evaluated cellular-morphology activity of compounds by transmission electron microscopy (TEM) and measurement of apoptosis (phosphatidylserine expression) by flow cytometry. Results: EC50 of amphotericin B and BSF-CH3 were 0.50 mu M and 0.39 mu M respective. Other nitro-heterocyclic compounds presented EC50 higher than amphotericin B. All compounds showed greater AV - and PI-positive expression than amphotericin B at 100 mu M, except BSF-NO2. TEM showed complete nuclear disfigurement with 100 mu M of BSF-NO2, 25 and 6.25 mu M of BSF-H, and 6.25 mu M BSF-Cl; presence of vesicles within the flagellar pocket with 25 mu M BSF-H; alteration of the kinetoplast with 25 mu M BSF-C4H9, 25 mu M of BSF-H, 6.25 mu M BSF-CH3 and 6.25 mu M of BSF-Cl. Conclusions: Nitro-heterocyclic compounds have shown activity against promastigotes of L. amazonensis, at lower concentrations. However, improvement of compound scaffolds are needed to assist the elucidation of the mechanism of action and to achieve greater activity.
  • conferenceObject
    Specie-specific diferenciation of Leishmania through real time PCR and high resolution melt
    (2015) SILVA, R. E. C.; FARIAS, L.; DIAS, D. B.; SILVA, E. O. Mello; SOARES, S. R. C.; LINDOSO, J. A. L.
  • article 14 Citação(ões) na Scopus
    Effects of nitro-heterocyclic derivatives against Leishmania (Leishmania) infantum promastigotes and intracellular amastigotes
    (2016) SILVA, Simone Carolina Soares Petri e; PALACE-BERL, Fanny; TAVARES, Leoberto Costa; SOARES, Sandra Regina Castro; LINDOSO, Jose Angelo Lauletta
    Leishmaniasis is an overlooked tropical disease affecting approximately 1 million people in several countries. Clinical manifestation depends on the interaction between Leishmania and the host's immune response. Currently available treatment options for leishmaniasis are limited and induce severe side effects. In this research, we tested nitro-heterocyclic compounds (BSF series) as a new alternative against Leishmania. Its activity was measured in Leishmania (Leishmania) infantum promastigotes and intracellular amastigotes using MIT colorimetric assay. Additionally, we assessed the phosphatidylserine exposure by promastigotes, measured by flow cytometry, as well as nitric oxide production, measured by Griess' method. The nitro-heterocyclic compounds (BSF series) showed activity against L. (L.) infantum promastigotes, inducting the phosphatidylserine exposition by promastigotes, decreasing intracellular amastigotes and increasing oxide nitric production. The selectivity index was more prominent to Leishmania than to macrophages. Compared to amphotericin b, our compounds presented higher IC50, however the selectivity index was more specific to parasite than to amphotericin b. In conclusion, these nitro-heterocyclic compounds showed to be promising as an anti-Leishmania drug, in in vitro studies.