VINICIUS DAGUANO GASTALDI

(Fonte: Lattes)
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Lattes
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Instituto de Psiquiatria, Hospital das Clínicas, Faculdade de Medicina
LIM/23 - Laboratório de Psicopatologia e Terapêutica Psiquiátrica, Hospital das Clínicas, Faculdade de Medicina

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  • article 25 Citação(ões) na Scopus
    C-4 and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation
    (2020) FERRARI, Renata C.; BITTENCOURT, Priscila P.; RODRIGUES, Maria A.; MORENO-VILLENA, Jose J.; ALVES, Frederico R. R.; GASTALDI, Vinicius D.; BOXALL, Susanna F.; DEVER, Louisa V.; DEMARCO, Diego; ANDRADE, Sonia C. S.; EDWARDS, Erika J.; HARTWELL, James; FRESCHI, Luciano
    Although biochemically related, C-4 and crassulacean acid metabolism (CAM) systems are expected to be incompatible. However, Portulaca species, including P. oleracea, operate C-4 and CAM within a single leaf, and the mechanisms behind this unique photosynthetic arrangement remain largely unknown. Here, we employed RNA-seq to identify candidate genes involved exclusively or shared by C-4 or CAM, and provided an in-depth characterization of their transcript abundance patterns during the drought-induced photosynthetic transitions in P. oleracea. Data revealed fewer candidate CAM-specific genes than those recruited to function in C-4. The putative CAM-specific genes were predominantly involved in night-time primary carboxylation reactions and malate movement across the tonoplast. Analysis of gene transcript-abundance regulation and photosynthetic physiology indicated that C-4 and CAM coexist within a single P. oleracea leaf under mild drought conditions. Developmental and environmental cues were shown to regulate CAM expression in stems, whereas the shift from C-4 to C-4-CAM hybrid photosynthesis in leaves was strictly under environmental control. Moreover, efficient starch turnover was identified as part of the metabolic adjustments required for CAM operation in both organs. These findings provide insights into C-4/CAM connectivity and compatibility, contributing to a deeper understanding of alternative ways to engineer CAM into C-4 crop species.
  • article 3 Citação(ões) na Scopus
    Environmental Influences Measured by Epigenetic Clock and Vulnerability Components at Birth Impact Clinical ASD Heterogeneity
    (2021) REIS, Viviane Neri de Souza; TAHIRA, Ana Carolina; GASTALDI, Vinicius Daguano; MARI, Paula; PORTOLESE, Joana; SANTOS, Ana Cecilia Feio dos; LISBOA, Bianca; MARI, Jair; CAETANO, Sheila C.; BRUNONI, Decio; BORDINI, Daniela; PAULA, Cristinane Silvestre de; VENCIO, Ricardo Z. N.; QUACKENBUSH, John; BRENTANI, Helena
    Although Autism Spectrum Disorders (ASD) is recognized as being heavily influenced by genetic factors, the role of epigenetic and environmental factors is still being established. This study aimed to identify ASD vulnerability components based on familial history and intrauterine environmental stress exposure, explore possible vulnerability subgroups, access DNA methylation age acceleration (AA) as a proxy of stress exposure during life, and evaluate the association of ASD vulnerability components and AA to phenotypic severity measures. Principal Component Analysis (PCA) was used to search the vulnerability components from 67 mothers of autistic children. We found that PC1 had a higher correlation with psychosocial stress (maternal stress, maternal education, and social class), and PC2 had a higher correlation with biological factors (psychiatric family history and gestational complications). Comparing the methylome between above and below PC1 average subgroups we found 11,879 statistically significant differentially methylated probes (DMPs, p < 0.05). DMPs CpG sites were enriched in variably methylated regions (VMRs), most showing environmental and genetic influences. Hypermethylated probes presented higher rates in different regulatory regions associated with functional SNPs, indicating that the subgroups may have different affected regulatory regions and their liability to disease explained by common variations. Vulnerability components score moderated by epigenetic clock AA was associated with Vineland Total score (p = 0.0036, adjR(2) = 0.31), suggesting risk factors with stress burden can influence ASD phenotype.
  • article 3 Citação(ões) na Scopus
    DNA methylation mediates a randomized controlled trial home-visiting intervention during pregnancy and the Bayley infant's cognitive scores at 12 months of age
    (2022) EUCLYDES, Veronica L. V.; GASTALDI, Vinicius D.; FELTRIN, Arthur S.; HOFFMAN, Daniel J.; GOUVEIA, Gisele; COGO, Hugo; FELIPE-SILVA, Aloisio; VIEIRA, Rossana P.; MIGUEL, Euripedes C.; V, Guilherme Polanczyk; CHIESA, Anna; FRACOLLI, Lislaine; MATIJASEVICH, Alicia; FERRARO, Alexandre; ARGEU, Adriana; MASCHIETTO, Mariana; BRENTANI, Helena P.
    The crosstalk between maternal stress exposure and fetal development may be mediated by epigenetic mechanisms, including DNA methylation (DNAm). To address this matter, we collect 32 cord blood samples from low-income Brazilian pregnant adolescents participants of a pilot randomized clinical intervention study (ClinicalTrials.gov, Identifier: NCT02807818). We hypothesized that the association between the intervention and infant neurodevelopmental outcomes at 12 months of age would be mediated by DNAm. First, we searched genome methylation differences between cases and controls using different approaches, as well as differences in age acceleration (AA), represented by the difference of methylation age and birth age. According to an adjusted p-value <= 0.05 we identified 3090 differentially methylated positions- CpG sites (DMPs), 21 differentially methylated regions (DMRs) and one comethylated module weakly preserved between groups. The intervention group presented a smaller AA compared to the control group (p = 0.025). A logistic regression controlled by sex and with gestational age indicated a coefficient of -0.35 towards intervention group (p = 0.016) considering AA. A higher cognitive domain score from Bayley III scale was observed in the intervention group at 12 months of age. Then, we performed a potential causal mediation analysis selecting only DMPs highly associated with the cognitive domain (adj. R (2) > 0.4), DMRs and CpGs of hub genes from the weakly preserved comethylated module and epigenetic clock as raw values. DMPs in STXBP6, and PF4 DMR, mediated the association between the maternal intervention and the cognitive domain at 12 months of age. In conclusion, DNAm in different sites and regions mediated the association between intervention and cognitive outcome.