ANDERSON DA COSTA LINO COSTA
Índice h a partir de 2011
2
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Hepatocellular carcinoma may display elevated nestin expression in endothelial cells: experimental study(2015) NOGUEIRA, Adriano Barreto; NOGUEIRA, Ariel Barreto; COSTA, Anderson Lino; LIMA, Fabiana Roberto; SIQUEIRA, Sheila Aparecida; TEIXEIRA, Manoel JacobsenCONTEXT AND OBJECTIVE: Nestin, a class VI intermediate filament protein, is highly expressed in the portal mesenchyme and sinusoidal endothelium of the human fetal liver, but scarcely expressed in adult portal vessel endothelium. During experimental liver regeneration, an increased number of nestin-positive parenchymal cells have been observed in the zone adjacent to the Hering canals. These parenchymal cells are regarded as hepatic stem cells or hepatoblasts, which may be involved in hepatocellular carcinogenesis. In the light of recent reports describing nestin-positive parenchymal cells in hepatocellular carcinoma, we aimed to use this tumor type as a positive control for immunohistochemical detection of nestin. DESIGN AND SETTING: Experimental study conducted at a university hospital. METHODS: Hepatocellular carcinoma sections from one case were analyzed for nestin expression by immunohistochemistry using confocal microscopy. RESULTS: Surprisingly, a conspicuous pattern resembling liver sinusoid-like cytoarchitecture was observed upon nestin staining of endothelial cells. CONCLUSIONS: This pattern has not been previously described. The preliminary results shown here suggest that nestin-positive endothelial cells are located in niches of immature or proliferative cells. Moreover, nestin expression in endothelial cells of hepatocellular carcinoma enhances the role of angiogenesis in this tumor type, although the prevalence of this immunohistopathological pattern remains to be determined. Finally, hepatocellular carcinoma is an effective positive control for nestin staining in fluorescent immunohistochemistry.
conferenceObject Evidences that Anorectal Transplantation is the Logical Treatment for Serious Anorectal Dysfunction and Permanente Colostomy.(2015) GALVAO, Flavio; SEID, Victor E.; WAISBERG, Daniel R.; COSTA, Anderson C.; LANCHOTTE, Cinthia; BAPTISTA, Rachel R.; NAITO, Chaib E. Munekazu; ARAKI, Jun; D'ALBUQUERQUE, Luiz A.conferenceObject Prevalence association between HPV and fistulising perianal Crohn's disease(2020) BOARINI, L. Rodrigues; SOBRADO JR., C. W.; GUZELA, V. R.; GONCALVES, A. Pozzebon; VILLA, L. Lina; NADAL, S. R.; COSTA, A. Da Costa Lino; NAHAS, S. C.- Immunoglobulin G profile in hyperacute rejection after multivisceral xenotransplantation(2012) GALVAO, Flavio H. F.; SOLER, Wangles; POMPEU, Eduardo; WAISBERG, Daniel R.; MELLO, Evandro S. D.; COSTA, Anderson C. L.; TEODORO, Walcy; VELOSA, Ana P.; CAPELOZZI, Vera L.; ANTONANGELO, Leila; CATANOZI, Sergio; MARTINS, Alessandro; MALBOUISSON, Luiz M. S.; CRUZ JR., Ruy J.; FIGUEIRA, Estela R.; FILHO, Joel A. R.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz A. C.Galvao FHF, Soler W, Pompeu E, Waisberg DR, Mello ES, Costa ACL, Teodoro W, Velosa AP, Capelozzi VL, Antonangelo L, Catanozi S, Martins A, Malbouisson LMS, Cruz RJ, Figueira ER, Filho JAR, Chaib E, D'Albuquerque LAC. Immunoglobulin G profile in hyperacute rejection after multivisceral xenotransplantation. Xenotransplantation 2012; 19: 298304. (c) 2012 John Wiley & Sons A/S. Abstract: Introduction: Xenotransplantation is a potential solution for the high mortality of patients on the waiting list for multivisceral transplantation; nevertheless, hyperacute rejection (HAR) hampers this practice and motivates innovative research. In this report, we describe a model of multivisceral xenotransplantation in which we observed immunoglobulin G (IgG) involvement in HAR. Methods: We recovered en bloc multivisceral grafts (distal esophagus, stomach, small intestine, colon, liver, pancreas, and kidneys) from rabbits (n = 20) and implanted them in the swine (n = 15) or rabbits (n = 5, control). Three hours after graft reperfusion, we collected samples from all graft organs for histological study and to assess IgG fixation by immunofluorescence. Histopathologic findings were graded according to previously described methods. Results: No histopathological features of rejection were seen in the rabbit allografts. In the swine-to-rabbit grafts, features of HAR were moderate in the liver and severe in esophagus, stomach, intestines, spleen, pancreas, and kidney. Xenograft vessels were the central target of HAR. The main lesions included edema, hemorrhage, thrombosis, myosites, fibrinoid degeneration, and necrosis. IgG deposition was intense on cell membranes, mainly in the vascular endothelium. Conclusions: Rabbit-to-swine multivisceral xenotransplants undergo moderate HAR in the liver and severe HAR in the other organs. Moderate HAR in the liver suggests a degree of resistance to the humoral immune response in this organ. Strong IgG fixation in cell membranes, including vascular endothelium, confirms HAR characterized by a primary humoral immune response. This model allows appraisal of HAR in multiple organs and investigation of the livers relative resistance to this immune response.
article 2 Citação(ões) na Scopus Small bowel transplantation in outbred rats(2011) WAISBERG, Daniel Reis; LEE, Andre Dong Wong; SANTOS, Rafael Miyashiro Nunes dos; MORY, Eduardo Kenji; COSTA, Anderson Lino; MONTERO, Edna Frasson de Souza; CHAIB, Eleazar; D'ALBUQUERQUE, Luis Augusto Carneiro; GALVAO, Flavio Henrique FerreiraPURPOSE: To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS: Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7(th) and 15(th) postoperative day and eight (22.2%) between 34(th) and 47(th) postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION: Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution.- Allogeneic anorectal transplantation in rats: technical considerations and preliminary results(2016) GALVAO, Flavio H. F.; WAISBERG, Daniel R.; SEID, Victor E.; COSTA, Anderson C. L.; CHAIB, Eleazar; BAPTISTA, Rachel Rossini; CAPELOZZI, Vera Luiza; LANCHOTTE, Cinthia; CRUZ, Ruy J.; ARAKI, Jun; D'ALBUQUERQUE, Luiz CarneiroFecal incontinence is a challenging condition with numerous available treatment modalities. Success rates vary across these modalities, and permanent colostomy is often indicated when they fail. For these cases, a novel potential therapeutic strategy is anorectal transplantation (ATx). We performed four isogeneic (Lewis-to-Lewis) and seven allogeneic (Wistar-to-Lewis) ATx procedures. The anorectum was retrieved with a vascular pedicle containing the aorta in continuity with the inferior mesenteric artery and portal vein in continuity with the inferior mesenteric vein. In the recipient, the native anorectal segment was removed and the graft was transplanted by end-to-side aorta-aorta and porto-cava anastomoses and end-to-end colorectal anastomosis. Recipients were sacrificed at the experimental endpoint on postoperative day 30. Surviving animals resumed normal body weight gain and clinical performance within 5 days of surgery. Isografts and 42.9% of allografts achieved normal clinical evolution up to the experimental endpoint. In 57.1% of allografts, signs of immunological rejection (abdominal distention, diarrhea, and anal mucosa inflammation) were observed three weeks after transplantation. Histology revealed moderate to severe rejection in allografts and no signs of rejection in isografts. We describe a feasible model of ATx in rats, which may allow further physiological and immunologic studies.