LUIZ FERNANDO PORTUGAL FUCHS
Projetos de Pesquisa
Unidades Organizacionais
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
9 resultados
Resultados de Busca
Agora exibindo 1 - 9 de 9
bookPart 0 Citação(ões) na Scopus Surgical pinealectomy in rats(2013) MAGANHIN, C. C.; SIMõES, R. S.; SIMõES, M. de Jesus; FUCHS, L. F. P.; BARACAT, E. C.; SOARES JR., J. M.To report a new, direct visual approach for rat pinealectomy. The rat was weighted and anesthetized intraperitoneally with 15 mg/kg xylazine and 30 mg/kg ketamine. The animal was fastened to a dissection table, an incision was made in the skin and the subcutaneous tissue, bringing the lambda into view, under a stereomicroscope with magnification 10 - 40x. The skullcap was opened with a dental drill, bringing the cerebral hemispheres and the superior sagittal sinus into view. The pineal gland, located under the venous sinus, was removed in a single piece using tweezers. Next, the bone fragment was returned to its place and the surgical layers were sutured. This new technique is easy to be done, avoids bleedings and removes only the pineal gland without damage to the remaining encephalon. In addition it makes possible the achievement of a sham surgery, allowing the pineal gland to remain intact. The proposed technique intends to facilitate studies aiming to better understanding the complexity and importance of the pineal gland on reproductive and other body systems. © 2013 Nova Science Publishers, Inc. All rights reserved.- Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation(2015) LIMA, Gisele Negro; MAGANHIN, Carla Cristina; SIMOES, Ricardo Santos; BARACAT, Maria Candida Pinheiro; SASSO, Gisela Rodrigues da Silva; FUCHS, Luiz Fernando Portugal; SIMOES, Manuel de Jesus; BARACAT, Edmund Chada; SOARES JUNIOR, Jose MariaOBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 mu g/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip (R) Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip (R) Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis.
conferenceObject Imunoexpression of BCL-2 and BAX in ovarian follicles of pinealectomized or melatonin-treated pinealectomized rats(2015) FUCHS, Luiz F.; MAGANHIN, Carla C.; SASSO, Gisela R.; SIMOES, Ricardo S.; BARACAT, Edmund C.; SOARES- JR., Jose Maria- Effects of melatonin on ovarian follicles(2013) MAGANHIN, Carla Cristina; FUCHS, Luiz Fernando Portugal; SIMOES, Ricardo Santos; OLIVEIRA-FILHO, Ricardo Martins; SIMOES, Manuel de Jesus; BARACAT, Edmund Chada; SOARES JR., Jose MariaObjective: To evaluate the histomorphometry and expression of Ki-67 and c-kit in ovarian follicles of pinealectomized or melatonin-treated pinealectomized rats. Study design: Forty adult rats were randomly divided into four groups of 10 animals: Group I - control; Group II - sham-pinealectomized; Group III - pinealectomized (Px), and Group IV - Px treated with melatonin (10 mu g/night, per animal). After two months' treatment, on the night of proestrous, the animals were placed in metabolic cages for night urine collection and subsequent measurement of 6-sulfatoxymelatonin (6-SMT). The rats were anesthetized, blood samples were taken for estrogen and progesterone determinations, and they were then euthanized. The ovaries were dissected out for further histological and immunohistochemical analyses. Data were first submitted to analysis of variance (ANOVA) complemented with the Tukey-Kramer test for multiple comparisons (P < 0.05). Results: The urinary levels of 6-SMT and serum progesterone were lower in the Px group (GIII). Exogenous melatonin treatment restored both blood melatonin and 6-SMT urinary levels. The histomorphometric data in Group III revealed a significant increase of degenerating antral and nonantral follicles with regard to the other groups. In addition no corpora lutea were observed in this group. No significant differences were noticed regarding the number of corpora lutea among the other groups (I, II and IV), but the number of cells and the thickness of the theca interna of Px animals (Group III) were higher than in the other groups. Conversely, the density of progesterone receptors (fmol/g) in the ovaries of Group III was significantly lower than in the other groups. Conclusion: Our data indicate that melatonin exerts a role on the maintenance of a proper follicular function, and is thus important for ovulation and progesterone production.
bookPart Pinealectomia em animais(2014) JúNIOR, José Maria Soares; MAGHANIN, Carla Cristina; FUCHS, Luiz Fernando Portugal; SIMõES, Ricardo dos Santos; BARACAT, Edmund ChadaconferenceObject Expression and influence ovarian function by the upregulation and downregulation of steroidogenesis related genes in ovary of pinealectomized rats in proestrous phase: Folistatin and Inhibin-beta A(2015) MAGANHIN, Carla C.; FUCHS, Luiz F.; SIMOES, Ricardo S.; SASSO, Gisela R.; BARACAT, Edmund C.; SOARES- JR., Jose Maria- Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats(2014) MAGANHIN, Carla C.; SIMOES, Ricardo S.; FUCHS, Luiz F. P.; SASSO, Gisela R. S.; SIMOES, Manuel J.; BARACAT, Edmund C.; SOARES JR., Jose M.Objective: To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats. Design: Experimental research. Setting: University research laboratory. Animal(s): Thirty female adult rats. Intervention(s): Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 mu g/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary. Main Outcome Measure(s): Biologic molecular study followed by immunohistochemical analysis. Result(s): The changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups. Conclusion(s): Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis. (C)2014 by American Society for Reproductive Medicine.
- Effects of metformin on the adrenal cortex of androgenized rats(2015) MACEDO, Lilian Alves; CARBONEL, Adriana Aparecida Ferraz; SIMOES, Ricardo Santos; FUCHS, Luiz Fernando Portugal; AMARAL, Vinicius Cestari do; SIMONCINI, Tommaso; SIMOES, Manuel Jesus Santos; BARACAT, Edmund Chada; SOARES JR., Jose MariaObjective: To evaluate the sex steroid profile and histomorphometry of the adrenal cortical zones of androgenized rats (wistar) with polycystic ovary syndrome treated with metformin. Study design: Thirty animals were divided into three groups: GC (regular estrous cycle), GPE (permanent estrus), and GPEM (permanent estrus + metformin 28 mg/kg for 50 days). At the end of this period, blood was collected for hormone measurement. The width of the adrenal cortical zones and the nuclear volumes were analyzed by histomorphometry. The ANOVA test was used in the statistical analysis. Results: The adrenal glands of the androgenized animals were larger and more intensely vascularized than those of the other groups. The concentration of androstenedione in GPE was higher than that in the other groups (0.4 +/- 0.1*>= 0.2 +/- 0.1 = 0.2 +/- 01, *p<50.05). The width of the zona glomerulosa and of the zona reticularis and their nuclear volumes were greater in GPE compared to those of the other groups (GPE*>GPEM = GC, *p<50.05). Conclusion: Metformin treatment may decrease the serum levels of androstenedione as well as the width and the nuclear volumes of the zona glomerulosa and of the zona reticularis in androgenized animals.
conferenceObject Imnunoexpression of estrogen receptors may decrease produce melatonin in ovaries of pinealectomized female rats(2015) MAGANHIN, Carla C.; FUCHS, Luiz F.; SASSO, Gisela R.; SIMOES, Ricardo S.; BARACAT, Edmund C.; SOARES JUNIOR, Jose M.