Surgical pinealectomy in rats

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Maganhin, C. C.; Simões, R. S.; de Jesus Simões, M.; Fuchs, L. F. P.; Baracat, E. C.; Soares Jr., J. M.. Surgical pinealectomy in rats. In: . STEP BY STEP EXPERIMENTAL PINEALECTOMY TECHNIQUES IN ANIMALS FOR RESEARCHERS: NOVA SCIENCE PUBLISHERS, INC., 2013. p.87-97.
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To report a new, direct visual approach for rat pinealectomy. The rat was weighted and anesthetized intraperitoneally with 15 mg/kg xylazine and 30 mg/kg ketamine. The animal was fastened to a dissection table, an incision was made in the skin and the subcutaneous tissue, bringing the lambda into view, under a stereomicroscope with magnification 10 - 40x. The skullcap was opened with a dental drill, bringing the cerebral hemispheres and the superior sagittal sinus into view. The pineal gland, located under the venous sinus, was removed in a single piece using tweezers. Next, the bone fragment was returned to its place and the surgical layers were sutured. This new technique is easy to be done, avoids bleedings and removes only the pineal gland without damage to the remaining encephalon. In addition it makes possible the achievement of a sham surgery, allowing the pineal gland to remain intact. The proposed technique intends to facilitate studies aiming to better understanding the complexity and importance of the pineal gland on reproductive and other body systems. © 2013 Nova Science Publishers, Inc. All rights reserved.
  1. Cairncross, K.D., Parks, A.A., Everitt, A.V., Destefanis, P., Maxwell, C.A., The use of hypothermia in surgical pinealectomy of the neonate rat (1989) Physiol. Behav, 45, pp. 663-665
  2. Dair, E.L., Simoes, R.S., Simões, M.J., Romeu, L.R., Oliveira-Filho, R.M., Haidar, M.A., Baracat, E.C., Soares Jr., J.M., Effects of melatonin on the endometrial morphology and embryo implantation in rats (2008) Fertil Steril, 89 (5 SUPPL.), pp. 1299-1305
  3. Dardes, R.C., Baracat, E.C., Simões, M.J., Modulation of estrous cycle and LH, FSH and melatonin levels by pinealectomy and sham-pinealectomy. female rats. Prog Neuropsychopharmacol (2000) Biol. Psychiatry, 24, pp. 441-453
  4. Díaz, E., Vázquez, N., Fernández, C., Durand, D., Lasaga, M., Debeljuk, L., Díaz, B., Seasonal variations of Substance P in the striatum of the female rat are affected by maternal and offspring pinealectomy (2011) Neurosci. Lett, 492, pp. 71-75
  5. Hoffman, R.A., Reiter, R.J.R.J., Rapid pinealectomy in hamsters and other small rodents (1965) Anat. Record, 24, pp. 83-89
  6. Jaworek, J., Zwirska-Korczala, K., Szklarczyk, J., Nawrot-Porabka, K., Leja-Szpak, A., Jaworek, A.K., Tomaszewska, R., Pinealectomy aggravates acute pancreatitis in the rat (2010) Pharmacol Rep, 62, pp. 864-873
  7. Karakas, A., Coskun, H., Kaya, A., Kücük, A., Gündüz, B., The effects of the intraamygdalar melatonin injections on the anxiety like behavior and the spatial memory performance in male Wistar rats (2011) Behav Brain Res, 222, pp. 141-150
  8. Kato, K., Murai, I., Asai, S., Matsuno, Y., Komuro, S., Kaneda, N., Iwasaki, A., Kuwayama, H., Protective role of melatonin and the pineal gland in modulating water immersion restraint stress ulcer in the rats (1998) J. Clin. Gastroenterol, 27 (SUPPL. 1), pp. S110-S5
  9. Kuszak, J., Rodin, M., A new technique of pinealectomy for adult rats (1977) Experientia, 33, pp. 283-284
  10. Maganhin, C.C., Carbonel, A.A., Hatty, J.H., Fuchs, L.F., Oliveira-Júnior, I.S., Simões Mde, J., Simões, R.S., Soares-Jr, J.M., Melatonin effects on the female genital system: a brief review (2008) Rev. Assoc. Med. Bras, 54, pp. 267-271
  11. Maganhin, C.C., Simões, R.S., Fuchs, L.F., Oliveira-Filho, R.M., Simões Mde, J., Evêncio Neto, J., Baracat, E.C., Soares Jr., J.M., Rat pinealectomy: a modified direct visual approach (2009) Acta. Cir. Bras, 24, pp. 321-324
  12. Panzan, M.Q., Soares Jr., J.M., Alves da Motta, E.L., Haapalainen, E.F., Simoes, M.J., Baptista, H.A., Baracat, E.C., Metoclopramide-induced hyperprolactinemia caused marked decline in pinopodes and pregnancy rates in mice (2006) Hum. Reprod, 21, pp. 2514-2520
  13. Pohlmeyer, G., Reuss, S., Baum, A., An improved technique for visually controlled pinealectomy in the rat (1994) J. Exp. Anim. Sci, 36, pp. 84-88
  14. Soares Jr., J.M., Simões, M.J., Oshima, C.T., Mora, O.A., De Lima, G.R., Baracat, E.C., Pinealectomy changes rat ovarian interstitial cell morphology and decreases progesterone receptor expression (2003) Gynecol Endocrinol, 17, pp. 115-123
  15. Soares Jr., J.M., Masana, M.I., Erşahin, Ç., Dubocovich, M.L., Functional melatonin receptors in rat ovaries at various stages of the estrous cycle (2003) Journal of Pharmacology and Experimental Therapeutics, 306, pp. 694-702
  16. Teixeira, A.A.C., Simões, M.J., Wanderley-Teixeira, V., Soares Jr., J.M., Evaluation of the implantation in pinealectomized and/or submitted to the constant illumination rats (2004) Int. J. Morphol, 22, pp. 189-194
  17. Vázquez, N.M., Debeljuk, L., Díaz, E.R., Álvarez, C.F., Díaz, B.L., Influence of maternal pineal gland on the developmental pattern of neurokinin A (NKA) and substance P (SP) in male-rat-offspring: relationship to the season of the year (2004) Neurosci. Lett, 368, pp. 243-248