ANGELA BATISTA GOMES DOS SANTOS
Índice h a partir de 2011
10
Projetos de Pesquisa
Unidades Organizacionais
LIM/59 - Laboratório de Biologia Celular, Hospital das Clínicas, Faculdade de Medicina
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conferenceObject Intrapleural Target Therapies (anti-VEGF And Anti-EGFR) Reduce Malignant Pleural Effusion And Morbidity In An Experimental Model(2016) TEIXEIRA, L. R.; ACENCIO, M. M. P.; ALVARENGA, V. A.; PUKA, J.; LEITE, H. F. Z.; MILSONI, P. F.; FERNEZLIAN, S. M.; SANTOS, A. B. G.; MEDEIROS, M. C. R.; MARCHI, E.; CAPELOZZI, V. L.- Repeated inhalation of crack-cocaine affects spermatogenesis in young and adult mice(2012) PIRES, A.; PIERI, P.; HAGE, M.; SANTOS, A. B. G.; MEDEIROS, M. C. R.; GARCIA, R. C. T.; YONAMINE, M.; HALLAK, J.; SALDIVA, P. H. N.; ZORZETTO, J. C.; BUENO, H. M. S.To investigate the effects of repeated crack-cocaine inhalation on spermatogenesis of pubertal and mature Balb/c mice, ten young (Y-ex) and ten adult (A(ex)) Balb/c mice were exposed to the smoke from 5 g of crack with 57.7% of pure cocaine in an inhalation chamber, 5 days/week for 2 months. The young (Y-c) and adult (A(c)) control animals (n = 10) were kept in a specially built and controlled animal house facility. The morphologic analysis of both testes of all animals included the analysis of quantitative and qualitative histologic parameters to assess the effect of crack-cocaine on spermatogenesis and Leydig cells. Apoptosis was determined by immunolabeling with caspase-3 antibodies. Compared to the Y-c animals, Y-ex animals showed a significant reduction in the number of stage VII tubules per testis (p = 0.02), Sertoli cells (p < 0.001) and elongated spermatids (p = 0.001). Comparisons between the Y-ex and A(ex) groups identified a significant reduction in the number of Sertoli cells (p < 0.001) and round spermatids (p < 0.001) in the Y-ex group and a significant increase in apoptotic Leydig cells (p = 0.04) in the A(ex) group. The experimental results indicate that crack-cocaine smoke inhalation induced spermatogenesis disruption in chronically exposed mice, particularly in pubertal mice.
- Toll-like receptors 2, 3 and 4 and thymic stromal lymphopoietin expression in fatal asthma(2012) FERREIRA, D. S.; ANNONI, R.; SILVA, L. F. F.; BUTTIGNOL, M.; SANTOS, A. B. G.; MEDEIROS, M. C. R.; ANDRADE, L. N. S.; YICK, C. Y.; STERK, P. J.; SAMPAIO, J. L. M.; DOLHNIKOFF, M.; WENZEL, S. E.; MAUAD, T.Background Airway inflammation in asthma involves innate immune responses. Toll-like receptors (TLRs) and thymic stromal lymphopoietin (TSLP) are thought to be involved in airway inflammation, but their expression in asthmatics both large and small airways has not been investigated. Objective To analyse the expression of TLR2, TLR3, TLR4 and TSLP in large and small airways of asthmatics and compare their expression in smoking and non-smoking asthmatics; to investigate whether TLR expression is associated with eosinophilic or neutrophilic airway inflammation and with Mycoplasma pneumoniae and Chlamydophila pneumoniae infection. Methods Using immunohistochemistry and image analysis, we investigated TLR2, TLR3, TLR4 and TSLP expression in large and small airways of 24 victims of fatal asthma, FA, (13 non-smokers, 11 smokers) and nine deceased control subjects (DCtrl). TLRs were also measured in 18 mild asthmatics (MA) and 12 healthy controls (HCtrl). M. pneumoniae and C. pneumoniae in autopsy lung tissue were analysed using real-time polymerase chain reaction. Airway eosinophils and neutrophils were measured in all subjects. Results Fatal asthma patients had higher TLR2 in the epithelial and outer layers of large and small airways compared with DCtrls. Smoking asthmatics had lower TLR2 levels in the inner and outer layers of the small airways than non-smoking asthmatics. TSLP was increased in the epithelial and outer layers of the large airways of FA. FA patients had greater TLR3 expression in the outer layer of large airways and greater TLR4 expression in the outer layer of small airways. Eosinophilic airway inflammation was associated with TLR expression in the epithelium of FA. No bacterial DNA was detected in FA or DCtrls. MA and HCtrls had only a small difference in TLR3 expression. Conclusions and Clinical Relevance Increased expression of TLR 2, 3 and 4 and TSLP in fatal asthma may contribute to the acute inflammation surrounding asthma deaths.
conferenceObject Cd23+(fc epsilon rii - Low Affinity Ige Receptor) Cells In Large Airways And Bronchopulmonary Lymph Nodes Of Fatal Asthma(2013) CAGNONI, E. F.; FERREIRA, D. S.; SILVA, L. F. Ferraz da; CARVALHO, A. N.; SANTOS, A. B. G.; MEDEIROS, M. R.; DOLHNIKOFF, M.; RABE, K. F.; MAUAD, T.- Aerobic exercise reduces asthma phenotype by inhibiting purinergic signaling and lymphatic organs hyperactivation(2017) OLIVEIRA JR., Manoel Carneiro de; DIAS, Alana dos Santos; ASSUMPCAO NETO, Erasmo; OLIVO, Clarice; SANTOS, Angela Batista; VITORETTI, Luana; OLIVEIRA, Ana Paula Ligeiro de; VIEIRA, Rodolfo de Paula
- Testicular pathology in fatal COVID-19: A descriptive autopsy study(2022) DUARTE-NETO, Amaro N.; TEIXEIRA, Thiago A.; CALDINI, Elia G.; KANAMURA, Cristina T.; GOMES-GOUVEA, Michele S.; SANTOS, Angela B. G. dos; MONTEIRO, Renata A. A.; PINHO, Joao R. R.; MAUAD, Thais; SILVA, Luiz F. F. da; SALDIVA, Paulo H. N.; DOLHNIKOFF, Marisa; LEITE, Katia R. M.; HALLAK, JorgeBackground Multi-organ damage is a common feature of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, going beyond the initially observed severe pneumonia. Evidence that the testis is also compromised is growing. Objective To describe the pathological findings in testes from fatal cases of COVID-19, including the detection of viral particles and antigens, and inflammatory cell subsets. Materials and methods Postmortem testicular samples were obtained by percutaneous puncture from 11 deceased men and examined by reverse-transcription polymerase chain reaction (RT-PCR) for RNA detection and by light and electron microscopy (EM) for SARS-CoV-2. Immunohistochemistry (IHC) for the SARS-CoV-2 N-protein and lymphocytic and histiocytic markers was also performed. Results Eight patients had mild interstitial orchitis, composed mainly of CD68+ and TCD8+ cells. Fibrin thrombi were detected in five cases. All cases presented congestion, interstitial edema, thickening of the tubular basal membrane, decreased Leydig and Sertoli cells with reduced spermatogenesis, and strong expression of vascular cell adhesion molecule (VCAM) in vessels. IHC detected SARS-Cov-2 antigen in Leydig cells, Sertoli cells, spermatogonia, and fibroblasts in all cases. EM detected viral particles in the cytoplasm of fibroblasts, endothelium, Sertoli and Leydig cells, spermatids, and epithelial cells of the rete testis in four cases, while RT-PCR detected SARS-CoV-2 RNA in three cases. Discussion and conclusion The COVID-19-associated testicular lesion revealed a combination of orchitis, vascular changes, basal membrane thickening, Leydig and Sertoli cell scarcity, and reduced spermatogenesis associated with SARS-CoV-2 local infection that may impair hormonal function and fertility in men.
- Anti-inflammatory Effects of Aerobic Exercise in Mice Exposed to Air Pollution(2012) VIEIRA, Rodolfo de Paula; TOLEDO, Alessandra Choqueta; SILVA, Lucas Bogaz; ALMEIDA, Francine Maria; DAMACENO-RODRIGUES, Nilsa Regina; CALDINI, Elia Garcia; SANTOS, Angela Batista Gomes; RIVERO, Dolores Helena; HIZUME, Deborah Camargo; LOPES, Fernanda Degobbi Tenorio Quirino Santos; OLIVO, Clarice Rosa; CASTRO-FARIA-NETO, Hugo Caire; MARTINS, Milton Arruda; SALDIVA, Paulo Hilario Nascimento; DOLHNIKOFF, MarisaPurpose: Exposure to diesel exhaust particles (DEP) results in lung inflammation. Regular aerobic exercise improves the inflammatory status in different pulmonary diseases. However, the effects of long-term aerobic exercise on the pulmonary response to DEP have not been investigated. The present study evaluated the effect of aerobic conditioning on the pulmonary inflammatory and oxidative responses of mice exposed to DEP. Methods: BALB/c mice were subjected to aerobic exercise five times per week for 5 wk, concomitantly with exposure to DEP (3 mg.mL (1); 10 mu L per mouse). The levels of exhaled nitric oxide, reactive oxygen species, cellularity, interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-alpha) were analyzed in bronchoalveolar lavage fluid, and the density of neutrophils and the volume proportion of collagen fibers were measured in the lung parenchyma. The cellular density of leukocytes expressing IL-1 beta, keratinocyte chemoattractant (KC), and TNF-alpha in lung parenchyma was evaluated with immunohistochemistry. The levels of IL-1 beta, KC, and TNF-alpha were also evaluated in the serum. Results: Aerobic exercise inhibited the DEP-induced increase in the levels of reactive oxygen species (P < 0.05); exhaled nitric oxide (P < 0.01); total (P < 0.01) and differential cells (P < 0.01); IL-6 and TNF-alpha levels in bronchoalveolar lavage fluid (P < 0.05); the level of neutrophils (P < 0.001); collagen density in the lung parenchyma (P < 0.05); the levels of IL-6, KC, and TNF-alpha in plasma (P < 0.05); and the expression of IL-1 beta, KC, and TNF-alpha by leukocytes in the lung parenchyma (P < 0.01). Conclusions: We conclude that long-term aerobic exercise presents protective effects in a mouse model of DEP-induced lung inflammation. Our results indicate a need for human studies that evaluate the pulmonary responses to aerobic exercise chronically performed in polluted areas.
conferenceObject Adventitial Remodeling Of Experimental Pulmonary Hypertension Induces Myofibroblast-Produced Collagen V In A Th17-Pathways-Related Manner(2016) FABRO, A. T.; MACHADO, J. R.; TEODORO, W. R.; SANTOS, A. L. Dos; SANTOS, A. A. Dos; CRUNIVEL, H. R.; MILSONI, P. F.; FERNEZLIAN, S. D. M.; SANTOS, A. B. G. Dos; OLIVEIRA, R. A.; MEDEIROS, M. C. R.; RAINHO, C. A.; CAPELOZZI, V. L.- Yellow fever and orthotopic liver transplantation: new insights from the autopsy room for an old but re-emerging disease(2019) DUARTE-NETO, Amaro N.; CUNHA, Marielton dos P.; MARCILIO, Izabel; SONG, Alice T. W.; MARTINO, Rodrigo B. de; HO, Yeh-Li; POUR, Shahab Z.; DOLHNIKOFF, Marisa; SALDIVA, Paulo H. N.; DUARTE, Maria I. S.; TAKAKURA, Cleusa F.; LIMA, Fabiana R.; TANIGAWA, Ryan Y.; IGLEZIAS, Silvia D'A; KANAMURA, Cristina T.; SANTOS, Angela B. G. dos; PERONDI, Beatriz; ZANOTTO, Paolo M. de A.; D'ALBUQUERQUE, Luiz A. C.; ALVES, Venancio A. F.Aims The clinical spectrum of yellow fever (YF) ranges from asymptomatic to fulminant hepatitis. During the sylvatic YF epidemic in the metropolitan area of Sao Paulo, Brazil in 2018, seven orthotopic liver transplantations (OLTs) were performed in our institution to treat fulminant YF hepatitis. Three patients recovered, while four patients died following OLT. The autopsy findings of all these cases are presented herein as the first description of YF in transplanted patients. Methods and results All patients were men, aged 16-40 years, without vaccination to YF virus (YFV). All organs were examined, with tissue sampling for histopathological analysis. Detection of YF virus antigens (YFV Ag) was performed with two primary antibodies (mouse polyclonal anti-YFV antibody directed to wild strain and a goat anti-YF virus antibody), and RT-PCR assays were utilised to detect YFV-RNA. All the cases depicted typical findings of YF hepatitis in the engrafted liver. The main extrahepatic findings were cerebral oedema, pulmonary haemorrhage, pneumonia, acute tubular necrosis and ischaemic/reperfusion pancreatitis. Of the four cases, the YVF Ag was detected in the heart in one case, liver and testis in three cases, and the kidney and spleen in all four cases. All four cases had YF virus RNA detected by RT-PCR in the liver and in other organs. Conclusions Infection of the engrafted liver and other organs by YFV, possibly combined with major ischaemic systemic lesions, may have led to the death of four of the seven patients undergoing OLT.
- Airway epithelium mediates the anti-inflammatory effects of exercise on asthma(2011) VIEIRA, Rodolfo Paula; TOLEDO, Alessandra Choqueta de; FERREIRA, Sergio Cesar; SANTOS, Angela Batista Gomes dos; MEDEIROS, Maria Cristina Rodrigues; HAGE, Marcia; MAUAD, Thais; MARTINS, Milton de Arruda; DOLHNIKOFF, Marisa; CARVALHO, Celso Ricardo Fernandes deAirway epithelium plays an important role in the asthma physiopathology. Aerobic exercise decreases Th2 response in murine models of allergic asthma, but its effects on the structure and activation of airway epithelium in asthma are unknown. BALB/c mice were divided into control, aerobic exercise, ovalbumin-sensitized and ovalbumin-sensitized plus aerobic exercise groups. Ovalbumin sensitization occurred on days 0, 14, 28, 42, and aerosol challenge from day 21 to day 50. Aerobic exercise started on day 22 and ended on day 50. Total cells and eosinophils were reduced in ovalbumin-sensitized group submitted to aerobic exercise. Aerobic exercise also reduced the oxidative and nitrosative stress and the epithelial expression of Th2 cytokines, chemokines, adhesion molecules, growth factors and NF-kB and P2X7 receptor. Additionally, aerobic exercise increased the epithelial expression of IL-10 in non-sensitized and sensitized animals. These findings contribute to the understanding of the beneficial effects of aerobic exercise for chronic allergic airway inflammation, suggesting an immune-regulatory role of exercise on airway epithelium.