BRUNO RODRIGUES
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- Aerobic Exercise Training Delays Cardiac Dysfunction and Improves Autonomic Control of Circulation in Diabetic Rats Undergoing Myocardial Infarction(2012) RODRIGUES, Bruno; JORGE, Luciana; MOSTARDA, Cristiano T.; ROSA, Kaleizu T.; MEDEIROS, Alessandra; MALFITANO, Christiane; SOUZA JR., Alcione L. de; VIEGAS, Katia Apareceda da Silva; LACCHINI, Silvia; CURI, Rui; BRUM, Patricia C.; ANGELIS, Katia De; IRIGOYEN, Maria ClaudiaBackground: Exercise training (ET) has been used as a nonpharmacological strategy for treatment of diabetes and myocardial infarction (MI) separately. We evaluated the effects ET on functional and molecular left ventricular (LV) parameters as well as on autonomic function and mortality in diabetics after MI. Methods and Results: Male Wistar rats were divided into control (C), sedentary-diabetic infarcted (SDI), and trained-diabetic infarcted (TDI) groups. MI was induced after 15 days of streptozotocin-diabetes induction. Seven days after MI, the trained group underwent ET protocol (90 days, 50-70% maximal oxygen consumption-VO(2)max). LV function was evaluated noninvasively and invasively; baroreflex sensitivity, pulse interval variability, cardiac output, tissue blood flows, VEGF mRNA and protein, HIF1-alpha mRNA, and Ca2+ handling proteins were measured. MI area was reduced in TDI (21 +/- 4%) compared with SDI (38 +/- 4%). ET induced improvement in cardiac function, hemodynamics, and tissue blood flows. These changes were probable consequences of a better expression of Ca2+ handling proteins, increased VEGF mRNA and protein expression as well as improvement in autonomic function, that resulted in reduction of mortality in TDI (33%) compared with SDI (68%) animals. Conclusions: ET reduced cardiac and peripheral dysfunction and preserved autonomic control in diabetic infarcted rats. Consequently, these changes resulted in improved VO(2)max and survival after MI.
- Ventricular and autonomic benefits of exercise training persist after detraining in infarcted rats(2013) BARBOZA, Catarina Andrade; ROCHA, Leandro Yanase; MOSTARDA, Cristiano Teixeira; FIGUEROA, Diego; CAPERUTO, Erico Chagas; ANGELIS, Katia De; IRIGOYEN, Maria Claudia; RODRIGUES, BrunoWe evaluate the effects of detraining (DT, for 1 month) on the left ventricular (LV) remodeling and function, hemodynamic and baroreflex sensitivity (BRS), as well as on mortality rate of infarcted (MI) rats after 3 months of exercise training (ET, 50-70 % of VO2max). Male Wistar rats were divided into five groups: control (C, n = 10), untrained-infarcted (UI, n = 15), trained-infarcted (TI, n = 12), untrained-infarcted plus 1 month (UI-1, n = 15) and detrained-infarcted 1 month (DI-1, n = 15). LV function was evaluated by echocardiography at the initial and final of the protocols. After following, ET and/or DT protocols, hemodynamic and BRS [by tachycardic (TR) and bradycardic (BR) responses] were assessed. TI group displayed increased VO2max in comparison with UI and DI-1 groups; however, DI-1 values remained increased compared to UI-1 group. MI area was reduced by ET and maintained after DT. Ejection fraction (TI = 60 +/- A 2 and DI-1 = 61 +/- A 2 % vs. UI = 41 +/- A 1 and UI-1 = 37 +/- A 3 %), E/A ratio (TI = 1.6 +/- A 0.1 and DI-1 = 1.9 +/- A 0.1 vs. UI = 2.9 +/- A 0.2 and UI-1 = 2.9 +/- A 0.3), TR (TI = 3.3 +/- A 0.3 and DI-1 = 3.3 +/- A 0.4 vs. UI = 1.7 +/- A 0.1 and UI-1 = 1.6 +/- A 0.1 bpm/mmHg) and BR (TI = -2.2 +/- A 0.1 and DI-1 = -2.0 +/- A 0.1 vs. UI = -1.3 +/- A 0.09 and UI-1 = -1.2 +/- A 0.09 bpm/mmHg) were improved by ET and maintained after DT in comparison with untrained rats. These changes resulted in mortality reduction in the TI (8 %) and DI-1 groups (13 %) compared with the UI (46 %) and UI-1 (53 %) groups. These findings indicate that ET is not only an effective tool in the management of cardiovascular and autonomic MI derangements, but also that these positive changes were maintained even after 1 month of DT in rats.
- Exercise training prevents diastolic dysfunction induced by metabolic syndrome in rats(2012) MOSTARDA, Cristiano; MORAES-SILVA, Ivana Cinthya; SALEMI, Vera Maria Cury; MACHI, Jacqueline Freire; RODRIGUES, Bruno; ANGELIS, Katia De; FARAH, Vera de Moura Azevedo; IRIGOYEN, Maria ClaudiaOBJECTIVE: High fructose consumption contributes to the incidence of metabolic syndrome and, consequently, to cardiovascular outcomes. We investigated whether exercise training prevents high fructose diet-induced metabolic and cardiac morphofunctional alterations. METHODS: Wistar rats receiving fructose overload (F) in drinking water (100 g/l) were concomitantly trained on a treadmill (FT) for 10 weeks or kept sedentary. These rats were compared with a control group (C). Obesity was evaluated by the Lee index, and glycemia and insulin tolerance tests constituted the metabolic evaluation. Blood pressure was measured directly (Windaq, 2 kHz), and echocardiography was performed to determine left ventricular morphology and function. Statistical significance was determined by one-way ANOVA, with significance set at p<0.05. RESULTS: Fructose overload induced a metabolic syndrome state, as confirmed by insulin resistance (F: 3.6 +/- 0.2 vs. C: 4.5 +/- 0.2 mg/dl/min), hypertension (mean blood pressure, F: 118 +/- 3 vs. C: 104 +/- 4 mmHg) and obesity (F: 0.31 +/- 0.001 vs. C: 0.29 +/- 0.001 g/mm). Interestingly, fructose overload rats also exhibited diastolic dysfunction. Exercise training performed during the period of high fructose intake eliminated all of these derangements. The improvements in metabolic parameters were correlated with the maintenance of diastolic function. CONCLUSION: The role of exercise training in the prevention of metabolic and hemodynamic parameter alterations is of great importance in decreasing the cardiac morbidity and mortality related to metabolic syndrome.
- Impact of myocardial infarction on cardiac autonomic function in diabetic rats(2013) RODRIGUES, Bruno; MOSTARDA, Cristiano T.; JORGE, Luciana; BARBOZA, Catarina A.; GRANS, Camilla F.; ANGELIS, Katia De; IRIGOYEN, Maria ClaudiaAims: We evaluated autonomic and hemodynamic parameters and maximal oxygen consumption (VO(2)max) as possible determinants of mortality in streptozotocin (STZ) diabetic rats after myocardial infarction (MI). Method: Male Wistar rats were divided into (n=8 of each): control sham (CS), diabetes sham (DS), MI (I), and diabetes +MI (DI). MI was induced 15 days after STZ induction. VO(2)max was measured at 3 (basal), 30, 60, and 91 days after MI. Hemodynamic and autonomic parameters were evaluated 92 days after MI. Results: MI area was similar in infarcted groups (similar to 44%). Mortality rate increased in the DI (70%) compared with I (53%) group. Cardiopulmonary baroreflex, sympathetic (48%) and vagal (33%) tonus, low frequency (LF) band (57%), and LF/high frequency (HF) band ratio (53%) were reduced in DI compared with I animals. Furthermore, cardiac output (CO), peripheral vascular resistance (PVR) impairment, and VO(2)max reductions were observed in the DI compared with the I group. Conclusions: Our data suggest that the CO and PVR changes as well as VO(2)max reduction were probably associated with additional cardiac autonomic control impairment, and, consequently, increased mortality rate in diabetic rats after a chronic myocardial infarction.