ROSSANA VERONICA MENDOZA LOPEZ
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
14 resultados
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- Plasma metabolomics of oral squamous cell carcinomas based on NMR and MS approaches provides biomarker identification and survival prediction(2023) POLACHINI, Giovana Mussi; CASTRO, Tialfi Bergamin de; SMARRA, Luis Fabiano Soares; HENRIQUE, Tiago; PAULA, Carlos Henrique Diniz de; SEVERINO, Patricia; LOPEZ, Rossana Veronica Mendoza; CARVALHO, Andre Lopes; ZERI, Ana Carolina de Mattos; SILVA, Ismael Dale Cotrim Guerreiro; TAJARA, Eloiza H.Metabolomics has proven to be an important omics approach to understand the molecular pathways underlying the tumour phenotype and to identify new clinically useful markers. The literature on cancer has illustrated the potential of this approach as a diagnostic and prognostic tool. The present study aimed to analyse the plasma metabolic profile of patients with oral squamous cell carcinoma (OSCC) and controls and to compare patients with metastatic and primary tumours at different stages and subsites using nuclear magnetic resonance and mass spectrometry. To our knowledge, this is the only report that compared patients at different stages and subsites and replicates collected in diverse institutions at different times using these methodologies. Our results showed a plasma metabolic OSCC profile suggestive of abnormal ketogenesis, lipogenesis and energy metabolism, which is already present in early phases but is more evident in advanced stages of the disease. Reduced levels of several metabolites were also associated with an unfavorable prognosis. The observed metabolomic alterations may contribute to inflammation, immune response inhibition and tumour growth, and may be explained by four nonexclusive views-differential synthesis, uptake, release, and degradation of metabolites. The interpretation that assimilates these views is the cross talk between neoplastic and normal cells in the tumour microenvironment or in more distant anatomical sites, connected by biofluids, signalling molecules and vesicles. Additional population samples to evaluate the details of these molecular processes may lead to the discovery of new biomarkers and novel strategies for OSCC prevention and treatment.
- Lipid profile as a new diagnostic marker in head and neck cancer(2022) CASTRO, T. B. D.; POLACHINI, G. M.; SMARRA, L. F. S.; HENRIQUE, T.; LOPEZ, R. V. M.; ZERI, A. C. D. M.; SILVA, I. D. C. G. D.; VANDENBOSCH, M.; HEEREN, R. M.; SILVA, E. H. Tajara da
- Lymph node or perineural invasion is associated with low miR-15a, miR-34c and miR-199b levels in head and neck squamous cell carcinoma(2016) SOUSA, Lucas O.; SOBRAL, Lays M.; MATSUMOTO, Camila S.; SAGGIORO, Fabiano P.; LOPEZ, Rossana V. M.; PANEPUCCI, Rodrigo A.; CURTI, Carlos; SILVA JR., Wilson A.; GREENE, Lewis J.; LEOPOLDINO, Andreia M.Background: MicroRNAs (miRNAs or miRs) are post-transcriptional regulators of eukaryotic cells and knowledge of differences in miR levels may provide new approaches to diagnosis and therapy. Methods: The present study measured the levels of nine miRs in head and neck squamous cell carcinomas (HNSCC) and determined whether clinical pathological features are associated with differences in miR levels. SET (I2PP2A) and PTEN protein levels were also measured, since their levels can be regulated by miR-199b and miR-21, respectively. Nine miRs (miR-15a, miR-21, miR-29b, miR-34c, miR-100, miR-125b, miR-137, miR133b and miR-199b) were measured by real time qRT-PCR in HNSCC samples from 32 patients and eight resection margins. SET (I2PP2A) and PTEN protein levels were estimated by immunohistochemistry in paired HNSCC tissues and their matched resection margins. Results: In HNSCC, the presence of lymph node invasion was associated with low miR-15a, miR-34c and miR-199b levels, whereas the presence of perineural invasion was associated with low miR-199b levels. In addition, miR-21 levels were high whereas miR-100 and miR-125b levels were low in HNSCC compared to the resection margins. When HNSCC line HN12, with or without knockdown of SET, were transfected with miR-34c inhibitor or miR-34cmimic, the miR-34c inhibitor increased cell invasion capacity while miR-34cmimic decreased the cell invasion. Conclusions: We showed that the levels of specific miRs in tumor tissue can provide insight into the maintenance and progression of HNSCC. General significance: MiRNAs are up-or down-regulated during cancer development and progression; they can be prognosis markers and therapeutic targets in HNSCC. (C) 2016 The Authors.
- Survival analysis of young adults from a Brazilian cohort of non-small cell lung cancer patients(2021) NICOLAU, Jessica Silva; LOPEZ, Rossana Veronica Mendoza; LUIZAGA, Carolina Terra de Moraes; RIBEIRO, Karina Braga; ROELA, Rosimeire Aparecida; MAISTRO, Simone; KATAYAMA, Maria Lucia Hirata; NATALINO, Renato Jose Mendonca; JR, Gilberto de Castro; NETO, Jose Eluf; FOLGUEIRA, Maria Aparecida Azevedo KoikeBackground: The influence of age at diagnosis in non-small cell lung cancer (NSCLC) prognosis is unclear. Objectives: To compare in a Brazilian cohort of NSCLC patients of different age groups: 1) The overall survival; 2) Clinical features and treatment options. Methods: This is a retrospective cohort study using a hospital-based registry, for NSCLC patients registered in years 2000-2009. Patients were grouped into three age groups: Young adults (YA: < 40 years), middle-aged (MA: 40-64 years) and elderly (E: >= 65 years). Kaplan-Meier was used to estimate overall survival and Cox regression for hazard ratios (HRs) and 95% confidence intervals. Results: 17,422 NSCLC patients were included: 370 YA (2.1%), 8,697 MA (49.9%) and 8,355 E (48.0%). Compared with older age groups, the YA group had a higher proportion of females, patients diagnosed with adenocarcinoma and metastatic disease (63.2%). Overall survival was longer in YA in the entire cohort and in all clinical stages (CSs) (p < 0.001). For YA, higher education level was a good prognosis factor (compared with illiterate and incomplete elementary); advanced or metastatic disease (compared with early-stage disease) and treatment based in radiotherapy or chemotherapy (CT) (without surgery), compared with treatment combinations with surgery, were poor prognostic factors. Young men (but not women) had lower HR of death compared with older groups; YA had lower HR of death in all CSs compared with patients from older groups. A higher percentage of YA were treated with surgery or CT in early-stage disease compared with older groups. Besides that, YA and MA patients treated with surgery or CT had a better prognosis than elderlies. Conclusions: In this Brazilian cohort of NSCLC patients, most young individuals were diagnosed with metastatic disease. YA presented longer survival than older age groups in all CSs, but mainly in CS I/II and III, where some patients may achieve long remissions or cure.
- Association Between Early Admission at School and Oral Health and Nutritional Status of Children in the City of Sao Paulo, Brazil(2021) SINCHEZ, Carlos Javier Arauzo; VILLAR, Betzabeth Slater; FRAIZ, Fabian Calixto; LOPEZ, Rossana Veronica Mendoza; BAVARESCO, Caren Serra; HADDAD, Ana EstelaObjective: To investigate the possible relationship between early admission to the school of children in early childhood and oral health conditions (OH) and nutritional status (NS). Material and Methods: Cross-sectional study conducted with 140 children aged 3-4 years, selected for convenience, in 4 public schools in the city of Sao Paulo, Brazil, during 2016, divided into children with early (IE) and late (IL) admission at school. Comparisons between groups were performed for the presence of overweight / obesity (OW / OB), caries lesions (CL), malocclusion (MO) and dental biofilm (DB), in addition to socioeconomic and dietary data. Multiple regression analysis was applied to determine the association between age of admission at school and OH and NS. Results: Children with IE had CL = 28.1%; DB = 46.9%; MO = 54.7% and OW / OB = 25.9%. Children with IE had IL = 29.8%; DB = 35.1%; MO = 61.4% and OW / OB = 30.8%. No significant association was found between age of admission at school and CL: 1.40 (0.53-3.73) 0.490; DB: 0.51 (0.22-1.16) 0.112; MO: 1.77 (0.77-4.05) 0.173 and OW / OB: 1.27 (0.55-2.92) 0.568, [OR (95% CI) p]. Conclusion: The age of admission at school of children in early childhood did not show a significant association with OH and NS.
- METNET: a phase II trial of metformin in patients with well-differentiated neuroendocrine tumours(2022) GLASBERG, Joao; TALANS, Aley; GIOLLO, Thomas Rivelli; RECCHIMUZZI, Debora Zachello; BEZERRA NETO, Joao Evangelista; LOPEZ, Rossana Veronica Mendonza; HOFF, Paulo Marcelo Gehm; RIECHELMANN, Rachel P.Background: Preclinical studies have suggested that metformin has anti-tumour effects, likely due to blockage of mammalian target of rapamycin pathway through adenosine monophosphate-activated protein kinase and decreased insulin levels. A retrospective study showed that metformin added to everolimus to treat type 2 diabetes mellitus offered longer progression-free survival (PFS) in patients with pancreatic neuroendocrine tumours (NET). Aim(s): To evaluate the efficacy and safety of metformin monotherapy in patients with advanced/metastatic well-differentiated NET (WD-NET) of gastroenteropancreatic (GEP) or pulmonary origin. Patients and methods: Single-arm phase II trial of metformin 850 mg PO twice daily until progression or intolerance for patients with progressive metastatic well-differentiated GEP or pulmonary NET. The primary endpoint was disease control rate (DCR) by RECIST 1.1 at 6 months. Secondary endpoints were response rate, PFS, toxicity and variations in glycaemic profiles (glycaemia, glycated haemoglobin and peptide C and insulin) at baseline, at 30 and 90 days. Results: From 2014 to 2019, 28 patients were enrolled: median age was 50 years; 84% had non-functional NET, 86% were of GEP origin and 62% had G2 NET. At the time of last follow-up, 26 patients had progression, with 13 (46%) presenting DCR at 6 months and a median PFS of 6.3 months (95% confidence interval: 3.2-9.3). There was no objective response, but one patient with refractory carcinoid syndrome had complete symptom relief, lasting for more than 5 years. Variations in glycaemic profiles were not associated with DCR at 6 months. Diarrhoea was the most common adverse event, being grade 3 or 4 in 10% of the cases. Conclusion: Metformin monotherapy offers modest anti-tumour activity in well-differentiated GEP or lung NET.
- Loss of Caveolin-1 Expression in Tumor Cells is Associated with Increased Aggressiveness and Cell Invasion in Oral Squamous Cell Carcinoma(2023) NASCIMENTO, Rebeca Barros; PAIVA, Katiucia Batista Silva; RISTELI, Maija; SILVA, Luiz Henrique Santos; RODINI, Camila Oliveira; RODRIGUES, Maria Fernanda Setubal Destro; CICCO, Rafael De; LOPEZ, Rossana Veronica Mendoza; SALO, Tuula Anneli; NUNES, Fabio Daumas; XAVIER, Flavia Calo AquinoBackgroundChanges in Caveolin-1 (CAV-1) expression are related to tumorigenesis. The aim of this study was to evaluate the role of CAV-1 in tumor progression in oral squamous cell carcinoma (SCC) tissue samples and the effect of CAV-1 silencing on two oral tongue SCC (OTSCC) cell lines (SCC-25, from a primary tumor, and HSC-3 from lymph node metastases).MethodsMycroarray hybridization, mRNA expression, and immunohistochemistry were performed on OSCC tissue samples and corresponding non-tumoral margin tissues. The effects of CAV-1 silencing (siCAV-1) on cell viability, membrane fluidity, on the expression of epithelial to mesenchymal transition (EMT) markers and on cell migration and invasion capacity of OTSCC cell lines were evaluated.ResultsMicroarray showed a greater CAV-1 expression (1.77-fold) in OSCC tumors than in non-tumoral tissues and 2.0-fold more in less aggressive OSCCs. However, significant differences in CAV-1 gene expression were not seen between tumors and non-tumoral margins nor CAV-1 with any clinicopathological parameters. CAV-1 protein was localized both in carcinoma and in spindle cells of the tumor microenvironment (TME), and CAV-1 positive TME cells were associated with smaller/more aggressive tumors, independent of the carcinoma cells' expression. Silencing of CAV-1 increased cell viability only in SCC-25 cells. It also stimulated the invasion of HSC-3 cells and increased ECAD and BCAT mRNA in these cells; however, the protein levels of the EMT markers were not affected.ConclusionDecreased expression of CAV-1 by tumor cells in OSCC and an increase in the TME were associated with increased cell invasiveness and tumor aggressiveness.
- Erratum: METNET: a phase II trial of metformin in patients with well-differentiated neuroendocrine tumours (ecancer (2022) 16:1369 DOI:10.3332/ecancer.2022.1369)(2022) GLASBERG, J.; TALANS, A.; GIOLLO, T. R.; RECCHIMUZZI, D. Z.; NETO, J. E. B.; LOPEZ, R. V. M.; HOFF, P. M. G.; RIECHELMANN, R. P.Thomás Rivelli Giollo is corrected to Thomás Giollo Rivelli. © 2022 The Author(s).
- The prognostic role of microRNA in epithelial ovarian cancer: A systematic review of literature with an overall survival meta-analysis(2020) FERREIRA, P.; ROELA, R.A.; LOPEZ, R.V.M.; ESTEVEZ-DIZ, M. Del PilarObjective: To accomplish a systematic review of literature with overall survival meta-analysis about the role of microRNA in epithelial ovarian cancer as prognostic and predictive factor to chemotherapy response. Methods: A search was conducted in the PubMed database, using the keywords ""microRNA"" and ""ovarian cancer"" or ""miRNA"" and ""ovarian cancer"". Original articles published before 02/02/2019 that had as main subject microRNA (miRNA) and ovarian cancer were included. We considered for inclusion only studies that associated microRNA to chemotherapy-related diagnosis, prognosis, or response in ovarian cancer. Results: The literature search returned 1,482 articles, 497 of which fulfilled inclusion criteria, yielding 350 miRNAs. The status of each miRNA was assessed in serum and tissue of ovarian cancer, benign tumors, and healthy tissue. The status of up-/downregulation of miRNAs was related to prognostic features (overall survival and disease-free survival) and response predictive features such as platinum and paclitaxel sensitivity/resistance. The miRNAs that had been cited three or more times were selected for prognostic and response predictive features analysis. Twelve miRNAs fulfilled all these criteria and were included in the overall survival meta-analysis. Conclusions: miRNAs affect virtually all mechanisms of carcinogenesis, working as either oncogenes or tumor suppressor genes. In this systematic review we identified miRNAs that may be related to prognosis, diagnosis, and chemotherapy sensitivity. The 12 miRNAs identified here should be included in future studies for validation. Copyright: Ferreira et al. © 2019 Via Medica. All rights reserved.
- Risk factors for head and neck cancer in more and less developed countries: Analysis from the INHANCE consortium(2023) GOYAL, Neerav; HENNESSY, Max; LEHMAN, Erik; LIN, Wenxue; AGUDO, Antonio; AHRENS, Wolfgang; BOCCIA, Stefania; BRENNAN, Paul; BRENNER, Hermann; CADONI, Gabriella; CANOVA, Cristina; CHEN, Chu; CONWAY, David; CURADO, Maria Paula; MASO, Luigino Dal; DAUDT, Alexander W.; EDEFONTI, Valeria; FABIANOVA, Eleonora; FERNANDEZ, Leticia; FRANCESCHI, Silvia; GARAVELLO, Werner; GILLISON, Maura; HAYES, Richard B.; HEALY, Claire; HERRERO, Rolando; HOLCATOVA, Ivana; KANDA, Jossy L.; KELSEY, Karl; HANSEN, Bo T.; KOIFMAN, Rosalina; LAGIOU, Pagona; VECCHIA, Carlo La; LEVI, Fabio; LI, Guojun; LISSOWSKA, Jolanta; LOPEZ, Rossana Mendoza; LUCE, Daniele; MACFARLANE, Gary; MATES, Dana; MATSUO, Keitaro; MCCLEAN, Michael; MENEZES, Ana; MENVIELLE, Gwenn; MORGENSTERN, Hal; MOYSICH, Kirsten; NEGRI, Eva; OLSHAN, Andrew F.; PANDICS, Tamas; POLESEL, Jerry; PURDUE, Mark; RADOI, Loredana; RAMROTH, Heribert; RICHIARDI, Lorenzo; SCHANTZ, Stimson; SCHWARTZ, Stephen M.; SERRAINO, Diego; SHANGINA, Oxana; SMITH, Elaine; STURGIS, Erich M.; SWIATKOWSKA, Beata; THOMSON, Peter; VAUGHAN, Thomas L.; VILENSKY, Marta; WINN, Deborah M.; WUNSCH-FILHO, Victor; YU, Guo-Pei; ZEVALLOS, Jose P.; ZHANG, Zuo-Feng; ZHENG, Tongzhang; ZNAOR, Ariana; BOFFETTA, Paolo; HASHIBE, Mia; LEE, Yuan-Chin A.; MUSCAT, Joshua E.Objective We analyzed the pooled case-control data from the International Head and Neck Cancer Epidemiology (INHANCE) consortium to compare cigarette smoking and alcohol consumption risk factors for head and neck cancer between less developed and more developed countries. Subjects and Methods The location of each study was categorized as either a less developed or more developed country. We compared the risk of overall head and neck cancer and cancer of specific anatomic subsites associated with cigarette smoking and alcohol consumption. Additionally, age and sex distribution between categories was compared. Results The odds ratios for head and neck cancer sites associated with smoking duration differed between less developed and more developed countries. Smoking greater than 20 years conferred a higher risk for oral cavity and laryngeal cancer in more developed countries, whereas the risk was greater for oropharynx and hypopharynx cancer in less developed countries. Alcohol consumed for more than 20 years conferred a higher risk for oropharynx, hypopharynx, and larynx cancer in less developed countries. The proportion of cases that were young (<45 years) or female differed by country type for some HNC subsites. Conclusion These findings suggest the degree of industrialization and economic development affects the relationship between smoking and alcohol with head and neck cancer.