NATHALIA BRANDAO GOBBATO
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- Antileukotriene Reverts the Early Effects of Inflammatory Response of Distal Parenchyma in Experimental Chronic Allergic Inflammation(2013) GOBBATO, Nathalia Brandao; SOUZA, Flavia Castro Ribas de; FUMAGALLI, Stella Bruna Napolitano; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; PRADO, Carla Maximo; MARTINS, Milton Arruda; TIBERIO, Iolanda de Fatima Lopes Calvo; LEICK, Edna AparecidaAims. Compare the effects of montelukast or dexamethasone in distal lung parenchyma and airway walls of guinea pigs (GP) with chronic allergic inflammation. Methods. GP have inhaled ovalbumin (OVA group-2x/week/4weeks). After the 4th inhalation, GP were treated with montelukast or dexamethasone. After 72 hours of the 7th inhalation, GP were anesthetised, and lungs were removed and submitted to histopathological evaluation. Results. Montelukast and dexamethasone treatments reduced the number of eosinophils in airway wall and distal lung parenchyma compared to OVA group (P < 0.05). On distal parenchyma, both treatments were effective in reducing RANTES, NF-kappa B, and fibronectin positive cells compared to OVA group (P < 0.001). Montelukast was more effective in reducing eotaxin positive cells on distal parenchyma compared to dexamethasone treatment (P < 0.001), while there was a more expressive reduction of IGF-I positive cells in OVA-D group (P < 0.001). On airway walls, montelukast and dexamethasone were effective in reducing IGF-I, RANTES, and fibronectin positive cells compared to OVA group (P < 0.05). Dexamethasone was more effective in reducing the number of eotaxin and NF-kappa B positive cells than Montelukast (P < 0.05). Conclusions. In this animal model, both treatments were effective in modulating allergic inflammation and remodeling distal lung parenchyma and airway wall, contributing to a better control of the inflammatory response.
conferenceObject Effects of corticosteroid and montelukast treatment in inflammation in guinea pigs with chronic allergic inflammation(2012) GOBBATO, Nathalia; SOUZA, Flavia; PRADO, Carla; MARTINS, Milton; TIBERIO, Iolanda; LEICK, Edna- Effects of corticosteroid, montelukast and iNOS inhibition on distal lung with chronic inflammation(2013) SOUZA, Flavia Castro Ribas; GOBBATO, Nathalia Brandao; MACIEL, Rafaela Guerra; PRADO, Carla Maximo; MARTINS, Milton Arruda; LEICK, Edna A.; TIBERIO, Iolanda F. L. CalvoWe evaluated the effects of anti-iNOS (1400W - W) associated with leukotriene antagonist (montelukast - M) or corticosteroid (dexamethasone - D) on distal lung of guinea pigs (GP) with chronic pulmonary inflammation. Methods: GP were inhaled with ovalbumin (OVA-2x/week/4 weeks), treated with M (OVAM), D (OVAD) and/or W (OVAW, OVADW, OVAMW) and distal lungs were evaluated by morphometry. Results: Isolated treatments were not sufficient to reduce all parameters. In OVADW, all parameters were reduced with greater reduction in elastic fibers, TIMP-1, IL-4, IL-5, IFN-gamma and PGF2-alpha compared with OVAD (p<0.05). OVAMW potentiated the reduction of actin, elastic fibers, TIMP-1, IL-4, IL-5, TGFbeta, IFN-gamma, iNOS, and PGF2-alpha to a greater extent than OVAM (p<0.05). A reduction of TIMP-1, IL-4, IL-5, TGF-beta, IFN-gamma and iNOS was observed in OVADW compared with OVAMW (p<0.05). Conclusions: Although anti-iNOS paired with montelukast or dexamethasone yields better results than isolated treatments, the most effective pairing for controlling inflammation, oxidative stress and remodeling in this asthma model was found to be corticosteroids and anti-iNOS. (C) 2012 Published by Elsevier B.V.