RENATA NISHIYAMA IKEGAMI
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
22 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 22
conferenceObject Infectious Agents Related with Collagen Degradation and Inflammation in Myxomatous Mitral Valve Degeneration(2016) SR., Marcos G. Tiveron; SR., Pablo M. A. Pomerantzeff; HIGUCHI, Maria L.; REIS, Marcia; PEREIRA, Jaqueline; KAWAKAMI, Joyce; IKEGAMI, Renata; SR., Carlos M. Brandao; SR., Fabio B. JateneconferenceObject SERUM ACTIVE COLLAGENASE FROM PATHOGENIC ARCHAEAL COLLAGENASE MAY CAUSE HEART FAILURE IN CHAGASIC PATIENTS(2019) HIGUCHI, Maria De Lourdes; KAWAKAMI, Joyce; IKEGAMI, Renata; REIS, Marcia; MORENO, Camila R.; PEREIRA, Jaqueline; IANNI, Barbara; BUCK, Paula; SANTOS, Marilia; BOCCHI, Edimar- Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes(2021) PEREIRA, Jaqueline de Jesus; IKEGAMI, Renata Nishiyama; KAWAKAMI, Joyce Tiyeko; GARAVELO, Sherrira Menezes; REIS, Marcia Martins; PALOMINO, Suely Aparecida Pinheiro; MANGINI, Sandrigo; MORENO, Camila Rodrigues; BARROS, Samar Freschi de; SOUZA, Aline Rodrigues; HIGUCHI, Maria de LourdesBackgroundIdiopathic dilated cardiomyopathy (IDCM) myocardial inflammation may be associated with external triggering factors such as infectious agents. Here, we searched if moderate/severe heart transplantation rejection is related to the presence of myocardial inflammation in IDCM explanted hearts, associated with microbial communities. MethodReceptor myocardial samples from 18 explanted hearts were separated into groups according to post-transplant outcome: persistent moderate rejection (PMR; n = 6), moderate rejection (MR; n = 7) that regressed after pulse therapy, and no rejection (NR; n = 5)/light intensity rejection. Inflammation was quantified through immunohistochemistry (IHC), and infectious agents were evaluated by IHC, molecular biology, in situ hybridization technique, and transmission electron microscopy (TEM). ResultsNR presented lower numbers of macrophages, as well as B cells (p = 0.0001), and higher HLA class II expression (p <= 0.0001). PMR and MR showed higher levels of Mycoplasma pneumoniae (p = 0.003) and hepatitis B core (p = 0.0009) antigens. NR presented higher levels of parvovirus B19 (PVB19) and human herpes virus 6 (HHV6) and a positive correlation between Borrelia burgdorferi (Bb) and enterovirus genes. Molecular biology demonstrated the presence of M. pneumoniae, Bb, HHV6, and PVB19 genes in all studied groups. TEM revealed structures compatible with the cited microorganisms. ConclusionsThis initial study investigating on infectious agents and inflammation in the IDCM explanted hearts showed that the association between M. pneumoniae and hepatitis B core was associated with a worse outcome after HT, represented by MR and PMR, suggesting that different IDCM microbial communities may be contributing to post-transplant myocardial rejection.
conferenceObject Lack of Protective Exosomes to Remove AMZ1 Archaeal Collagenase is Associated With Heart Failure in Chagas' Disease(2017) HIGUCHI, Maria de Lourdes; KAWAKAMI, Joyce T.; SANTOS, Marilia H.; IKEGAMI, Renata N.; REIS, Marcia M.; IANNI, Barbara; BUCK, Paula; BOCCHI, EdimarconferenceObject Fatal acute myocardial infarction related to lack of exosomes and low removal of free Mycoplasma pneumoniae lipoproteins(2017) HIGUCHI, M. L.; SANTOS, M. H. H.; IKEGAMI, R. N.; REIS, M. M.; KAWAKAMI, J. T.; PEREIRA, J. J.; PALOMINO, S. A. P.; BENSENOR, I. J. M.; LOTUFO, P. A.- Infectious agents is a risk factor for myxomatous mitral valve degeneration: A case control study(2017) TIVERON, Marcos Gradim; POMERANTZEFF, Pablo Maria Alberto; HIGUCHI, Maria de Lourdes; REIS, Marcia Martins; PEREIRA, Jaqueline de Jesus; KAWAKAMI, Joyce Tieko; IKEGAMI, Renata Nishiyama; BRANDAO, Carlos Manuel de Almeida; JATENE, Fabio BiscegliBackground: The etiology of myxomatous mitral valve degeneration (MVD) is not fully understood and may depend on time or environmental factors for which the interaction of infectious agents has not been documented. The purpose of the study is to analyze the effect of Mycoplasma pneumoniae (Mp), Chlamydophila pneumoniae (Cp) and Borrelia burgdorferi (Bb) on myxomatous mitral valve degeneration pathogenesis and establish whether increased in inflammation and collagen degradation in myxomatous mitral valve degeneration etiopathogenesis. Methods: An immunohistochemical test was performed to detect the inflammatory cells (CD20, CD45, CD68) and Mp, Bb and MMP9 antigens in two groups. The in situ hybridization was performed to detect Chlamydophila pneumoniae and the bacteria study was performed using transmission electron microscopy. Group 1 (n = 20), surgical specimen composed by myxomatous mitral valve degeneration, and group 2 (n = 20), autopsy specimen composed by normal mitral valve. The data were analyzed using SigmaStat version 20 (SPSS Inc., Chicago, IL, USA). The groups were compared using Student's t test, Mann-Whitney test. A correlation analysis was performed using Spearman's correlation test. P values lower than 0.05 were considered statistically significant. Results: By immunohistochemistry, there was a higher inflammatory cells/mm2 for CD20 and CD45 in group 1, and CD68 in group 2. Higher number of Mp and Cp antigens was observed in group 1 and more Bb antigens was detected in group 2. The group 1 exhibited a positive correlation between the Bb and MVD percentage, between CD45 and Mp, and between MMP9 with Mp. These correlations were not observed in the group 2. Electron microscopy revealed the presence of structures compatible with microorganisms that feature Borrelia and Mycoplasma characteristics. Conclusions: The presence of infectious agents, inflammatory cells and collagenases in mitral valves appear to contribute to the pathogenesis of MVD. Mycoplasma pneumoniae was strongly related with myxomatous mitral valve degeneration. Despite of low percentage of Borrelia burgdorferi in MD group, this agent was correlated with myxomatous degeneration and this may occour due synergistic actions between these infectious agents likely contribute to collagen degradation.
conferenceObject Bacteria and bacteriophages in the myocardium are related with resistence to pulse therapy in myocardial rejection(2014) REIS, M. M.; IKEGAMI, R. N.; FERREIRA, R. R. A.; KAWAKAMI, J. T.; PALOMINO, S. A. P.; POMERANTZEFF, P.; MANGINI, S.; BOCCHI, E. A.; HIGUCHI, M. L.- Archaea Symbiont of T. cruzi Infection May Explain Heart Failure in Chagas Disease(2018) HIGUCHI, Maria de Lourdes; KAWAKAMI, Joyce T.; IKEGAMI, Renata N.; REIS, Marcia M.; PEREIRA, Jaqueline de Jesus; IANNI, Barbara M.; BUCK, Paula; OLIVEIRA, Luanda Mara da Silva; SANTOS, Marilia H. H.; HAJJAR, Ludhmila A.; BOCCHI, Edimar A.Background: Archaeal genes present in Trypanosoma cruzi may represent symbionts that would explain development of heart failure in 30% of Chagas disease patients. Extracellular vesicles in peripheral blood, called exosomes (<0.1 mu m) or microvesicles (>0.1 mu m), present in larger numbers in heart failure, were analyzed to determine whether they are derived from archaea in heart failure Chagas disease. Methods: Exosomes and microvesicles in serum supernatant from 3 groups were analyzed: heart failure Chagas disease (N = 26), asymptomatic indeterminate form (N = 21) and healthy non-chagasic control (N = 16). Samples were quantified with transmission electron microscopy, flow cytometer immunolabeled with anti-archaemetzincin-1 antibody (AMZ 1, archaea collagenase) and probe anti-archaeal DNA and zymography to determine AMZ1 (Archaeal metalloproteinase) activity. Results: Indeterminate form patients had higher median numbers of exosomes/case vs. heart failure patients (58.5 vs. 25.5, P < 0.001), higher exosome content of AMZ1 antigens (2.0 vs. 0.0; P < 0.001), and lower archaeal DNA content (0.2 vs. 1.5, P = 0.02). A positive correlation between exosomes and AMZ1 content was seen in indeterminate form (r = 0.5, P < 0.001), but not in heart failure patients (r = 0.002, P = 0.98). Higher free archaeal DNA (63.0 vs. 11.1, P < 0.001) in correlation with exosome numbers (r = 0.66, P = 0.01) was seen in heart failure but not in indeterminate form (r = 0.29, P = 0.10). Flow cytometer showed higher numbers of AMZ1 microvesicles in indeterminate form (64 vs. 36, P = 0.02) and higher archaeal DNA microvesicles in heart failure (8.1 vs. 0.9, P < 0.001). Zymography showed strong% collagenase activity in HF group, mild activity in IF compared to non-chagasic healthy group (121 +/- 14, 106 +/- 13 and 100; P < 0.001). Conclusions: Numerous exosomes, possibly removing and degrading abnormal AMZ1 collagenase, are associated with indeterminate form. Archaeal microvesicles and their exosomes, possibly associated with release of archaeal AMZ1 in heart failure, are future candidates of heart failure biomarkers if confirmed in larger series, and the therapeutic focus in the treatment of Chagas disease.
- Infectious agents and inflammation in donated hearts and dilated cardiomyopathies related to cardiovascular diseases, Chagas' heart disease, primary and secondary dilated cardiomyopathies(2015) MANGINI, Sandrigo; HIGUCHI, Maria de Lourdes; KAWAKAMI, Joyce Tiyeko; REIS, Marcia Martins; IKEGAMI, Renata Nishiyama; PALOMINO, Suely Aparecida Pinheiro; POMERANTZEFF, Pablo Maria Alberto; FIORELLI, Alfredo Inacio; MARCONDES-BRAGA, Fabiana Goulart; BACAL, Fernando; FERREIRA, Silvia Moreira Ayub; ISSA, Victor Sarli; SOUZA, Germano Emilio Conceicao; CHIZZOLA, Paulo Roberto; BOCCHI, Edimar AlcidesBackground: Clinical and experimental conflicting data have questioned the relationship between infectious agents, inflammation and dilated cardiomyopathy (DCM). Objectives: The aim of this study was to determine the frequency of infectious agents and inflammation in endomyocardial biopsy (EMB) specimens from patients with idiopathic DCM, explanted hearts from different etiologies, including Chagas' disease, compared to donated hearts. Methods: From 2008 to 2011, myocardial samples from 29 heart donors and 55 patients with DCMs from different etiologies were studied (32 idiopathic, 9 chagasic, 6 ischemic and 8 other specific etiologies). Inflammation was investigated by immunohistochemistry and infectious agents by immunohistochemistry, molecular biology, in situ hybridization and electron microscopy. Results: There were no differences regarding the presence of macrophages, expression of HLA class II and ICAM-I in donors and DCM. Inflammation in Chagas' disease was predominant. By immunohistochemistry, in donors, there was a higher expression of antigens of enterovirus and Borrelia, hepatitis B and C in DCMs. By molecular biology, in all groups, the positivity was elevated to microorganisms, including co-infections, with a higher positivity to adenovirus and HHV6 in donors towards DCMs. This study was the first to demonstrate the presence of virus in the heart tissue of chagasic DCM. Conclusions: The presence of inflammation and infectious agents is frequent in donated hearts, in the myocardium of patients with idiopathic DCM, myocardial dysfunction related to cardiovascular diseases, and primary and secondary cardiomyopathies, including Chagas' disease. The role of co-infection in Chagas' heart disease physiopathology deserves to be investigated in future studies.
conferenceObject Archaeal DNA and heart failure in chagasic patients(2013) HIGUCHI, M. L.; IKEGAMI, R. N.; KAWAKAMI, J. T.; REIS, M. M.; SANTOS, M. H. H.; ISSA, V.; MANGINI, S.; BOCCHI, E. A.; KALIL FILHO, R.
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