FRANCINE MARIA DE ALMEIDA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
11 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 11
- Lodenafil treatment in the monocrotaline model of pulmonary hypertension in rats(2014) POLONIO, Igor Bastos; ACENCIO, Milena Marques Pagliareli; PAZETTI, Rogerio; ALMEIDA, Francine Maria de; SILVA, Brbara Soares da; PEREIRA, Karina Aparecida Bonifacio; SOUZA, RogerioWe assessed the effects of Iodenafil on hemodynamics and inflammation in the rat model of monocrotaline-induced pulmonary hypertension (PH). Thirty male Sprague-Dawley rats were randomly divided into three groups: control; monocrotaline (experimental model); and Iodenafila (experimental model followed by Iodenafil treatment, p.o., 5 mg/kg daily for 28 days) Mean pulmonary artery pressure (mPAP) was obtained by right heart catheterization. We investigated right ventricular hypertrophy (RVH) and IL-1 levels in lung fragments. The number of cases of RVH was significantly higher in the monocrotaline group than in the Iodenafil and control groups, as were mPAP and IL-1 levels. We conclude that Iodenafil can prevent monocrotaline-induced PH, RVH, and inflammation.
- Low level laser therapy reduces acute lung inflammation in a model of pulmonary and extrapulmonary LPS-induced ARDS(2014) OLIVEIRA JR., Manoel Carneiro; GREIFFO, Flavia Regina; RIGONATO-OLIVEIRA, Nicole Cristine; CUSTODIO, Ricardo Wesley Alberca; SILVA, Vanessa Roza; DAMACENO-RODRIGUES, Nilsa Regina; ALMEIDA, Francine Maria; ALBERTINI, Regiane; LOPES-MARTINS, Rodrigo Alvaro B.; OLIVEIRA, Luis Vicente Franco de; CARVALHO, Paulo de Tarso Camillo de; OLIVEIRA, Ana Paula Ligeiro de; LEAL JR., Ernesto Cesar P.; VIEIRA, Rodolfo P.The present study aimed to investigate the effects low level laser therapy (LLLT) in a LPS-induced pulmonary and extrapulmonary acute respiratory distress syndrome (ARDS) in BALB/c mice. Laser (830 nm laser, 9 J/cm(2), 35 mW, 80 s per point, 3 points per application) was applied in direct contact with skin, 1 h after LPS administration. Mice were distributed in control (n = 6; PBS), ARDS IT (n = 7; LPS orotracheally 10 mu g/mouse), ARDS IP (n = 7; LPS intra-peritoneally 100 mu g/mouse), ARDS IT + Laser (n = 9; LPS intra-tracheally 10 mu g/mouse), ARDS IP + Laser (n = 9; LPS intra-peritoneally 100 mu g/mouse). Twenty-four hours after last LPS administration, mice were studied for pulmonary inflammation by total and differential cell count in bronchoalveolar lavage (BAL), cytokines (IL-1 beta, IL-6, KC and TNF-alpha) levels in BAL fluid and also by quantitative analysis of neutrophils number in the lung parenchyma. LLLT significantly reduced pulmonary and extrapulmonary inflammation in LPS-induced ARDS, as demonstrated by reduced number of total cells (p < 0.001) and neutrophils (p < 0.001) in BAL, reduced levels of IL-1 beta, IL-6, KC and TNF-alpha in BAL fluid and in serum (p < 0.001), as well as the number of neutrophils in lung parenchyma (p < 0.001). LLLT is effective to reduce pulmonary inflammation in both pulmonary and extrapulmonary model of LPS-induced ARDS.
- Methylene blue attenuates ischemia-reperfusion injury in lung transplantation(2014) ABREU, Marcus da Matta; PAZETTI, Rogerio; ALMEIDA, Francine Maria de; CORREIA, Aristides Tadeu; PARRA, Edwin Roger; SILVA, Lais Pereira da; VIEIRA, Rodolfo de Paula; PEGO-FERNANDES, Paulo Manuel; JATENE, Fabio BiscegliBackground: Ischemia-reperfusion injury (IRI) is one of the principal obstacles for the lung transplantation (LTx) success. Several strategies have been adopted to minimize the effects of IRI in lungs, including ex vivo conditioning of the grafts and the use of antioxidant drugs, such as methylene blue (MB). We hypothesized that MB could minimize the effects of IRI in a LTx rodent model. Methods: Forty rats were divided into four groups (n = 10) according to treatment (saline solution or MB) and graft cold ischemic time (3 or 6 h). All animals underwent unilateral LTx. Recipients received 2 mL of saline or MB intraperitoneally before transplantation. After 2 h of reperfusion, arterial blood and exhaled nitric oxide samples were collected and bronchoalveolar lavage performed. Then animals were euthanized, and histopathology analysis as well as cell counts and cytokine levels measurements in bronchoalveolar lavage fluid were performed. Results: There was a significant decrease in exhaled nitric oxide, neutrophils, interleukin-6, and tumor necrosis factor-alpha in MB-treated animals. PaO2 and uric acid levels were higher in MB group. Conclusions: MB was able in attenuating IRI in this LTx model.
conferenceObject Effects of basiliximab and triple therapy on airway mucociliary clearance of rats(2014) CORREIA, Aristides Tadeu; ALMEIDA, Francine Maria; BENTO, Afonso da Silva Alves; HIRANO, Hugo Kenji Matsushima; PEGO-FERNANDES, Paulo Manuel; PAZETTI, RogerioconferenceObject Tacrolimus increases inflammatory cells in lung(2014) ALMEIDA, Francine Maria; SILVA, Maristela Prado e; SOTO, Sonia Fatima; CORREIA, Aristides Tadeu; PEGO-FERNANDES, Paulo; PAZETTI, RogerioconferenceObject Aerobic Exercise Attenuates Skeletal Muscle Injury Induced By Cigarette Smoke Exposure In Mice(2014) TOLEDO-ARRUDA, A. C.; SUEHIRO, C. L.; GUARNIER, F. A.; VIEIRA, R. D. P.; ALMEIDA, F. M.; LOPES, F. D.; ARANTES, P. D. M. M.; CECCHINI, R.; LIN, C. J.; MARTINS, M. D. A.- A Treatment with a Protease Inhibitor Recombinant from the Cattle Tick (Rhipicephalus Boophilus microplus) Ameliorates Emphysema in Mice(2014) LOURENCO, Juliana D.; NEVES, Luana P.; OLIVO, Clarice R.; DURAN, Adriana; ALMEIDA, Francine M.; ARANTES, Petra M. M.; PRADO, Carla M.; LEICK, Edna Aparecida; TANAKA, Aparecida S.; MARTINS, Milton A.; SASAKI, Sergio D.; LOPES, Fernanda D. T. Q. S.Aims: To determine whether a serine protease inhibitor treatment can prevent or minimize emphysema in mice. Methods: C57BL/6 mice were subjected to porcine pancreatic elastase (PPE) nasal instillation to induce emphysema and were treated with a serine protease inhibitor (rBmTI-A) before (Protocol 1) and after (Protocol 2) emphysema development. In both protocols, we evaluated lung function to evaluate the airway resistance (Raw), tissue damping (Gtis) and tissue elastance (Htis). The inflammatory profile was analyzed in the bronchoalveolar lavage (BALF) and through the use of morphometry; we measured the mean linear intercept (Lm) (to verify alveolar enlargement), the volume proportion of collagen and elastic fibers, and the numbers of macrophages and metalloprotease 12 (MMP-12) positive cells in the parenchyma. We showed that at both time points, even after the emphysema was established, the rBmTI-A treatment was sufficient to reverse the loss of elastic recoil measured by Htis, the alveolar enlargement and the increase in the total number of cells in the BALF, with a primary decrease in the number of macrophages. Although, the treatment did not control the increase in macrophages in the lung parenchyma, it was sufficient to decrease the number of positive cells for MMP-12 and reduce the volume of collagen fibers, which was increased in PPE groups. These findings attest to the importance of MMP-12 in PPE-induced emphysema and suggest that this metalloprotease could be an effective therapeutic target.
conferenceObject Effects of two immunosuppressive triple therapies on inflammatory cells in lung(2014) ALMEIDA, Francine Maria; SILVA, Maristela Prado e; SOTO, Sonia Fatima; CORREIA, Aristides Tadeu; PEGO-FERNANDES, Paulo; PAZETTI, Rogerio- Aerobic exercise attenuates pulmonary inflammation induced by Streptococcus pneumoniae(2014) OLIVO, Clarice R.; MIYAJI, Eliane N.; OLIVEIRA, Maria Leonor S.; ALMEIDA, Francine M.; LOURENCO, Juliana D.; ABREU, Rodrigo M.; ARANTES, Petra M. M.; LOPES, Fernanda D. T. Q. S.; MARTINS, Milton A.Aerobic exercise has been recognized as a stimulator of the immune system, but its effect on bacterial infection has not been extensively evaluated. We studied whether moderate aerobic exercise training prior to Streptococcus pneumoniae infection influences pulmonary inflammatory responses. BALB/c mice were divided into four groups: Sedentary Untreated (sedentary without infection); Sedentary Infected (sedentary with infection); Trained Untreated (aerobic training without infection); and Trained Infected (aerobic training with infection). Animals underwent aerobic training for 4 wk, and 72 h after last exercise training, animals received a challenge with S. pneumoniae and were evaluated either 12 h or 10 days after instillation. In acute phase, Sedentary Infected group had an increase in respiratory system resistance and elastance; number of neutrophils, lymphocytes, and macrophages in bronchoalveolar lavage fluid (BAL); polymorphonuclear cells in lung parenchyma; and levels of keratinocyte-derived chemokine (KC), tumor necrosis factor-alpha (TNF-alpha), and interleukin (IL) - 1 beta (IL-1 beta) in lung homogenates. Exercise training significantly attenuated the increase in all of these parameters and induced an increase in expression of antioxidant enzymes (CuZnSOD and MnSOD) in lungs. Trained Infected mice had a significant decrease in the number of colony-forming units of pneumococci in the lungs compared with Sedentary Infected animals. Ten days after infection, Trained Infected group exhibited lower numbers of macrophages in BAL, polymorphonuclear cells in lung parenchyma and IL-6 in lung homogenates compared with Sedentary Infected group. Our results suggest a protective effect of moderate exercise training against respiratory infection with S. pneumoniae. This effect is most likely secondary to an effect of exercise on oxidant-antioxidant balance.
conferenceObject Aerobic exercise decreases pulmonary and extrapulmonary LPS-induced ARDS through IL-10 and TLR-4 modulation(2014) RIGONATO-OLIVEIRA, Nicole Cristine; GREIFFO, Flavia Regina; CUSTODIO, Ricardo Wesley Alberca; ANDRADE-SOUSA, Adilson Santos; PEREIRA, Paulo Rogerio; ALMEIDA-OLIVEIRA, Ana Roberta; AQUINO-JUNIOR, Jefferson Commn Jonco; ALMEIDA, Francine Maria; DAMACENO-RODRIGUES, Nilsa Regina; LIGEIRO-OLIVEIRA, Ana Paula; ALBERTINI, Regiane; VIEIRA, Rodolfo Paula