MARA SANDRA HOSHIDA
Projetos de Pesquisa
Unidades Organizacionais
LIM/57 - Laboratório de Fisiologia Obstétrica, Hospital das Clínicas, Faculdade de Medicina
27 resultados
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Agora exibindo 1 - 10 de 27
conferenceObject Longitudinal serum levels of Placental Growth Factor and sFlt-1 throughout gestation in normotensive pregnancies and those complicated by hypertensive disorders(2013) COSTA, R. A.; HOSHIDA, M. S.; ALVES, E. A.; V, R. P. Francisco; ZUGAIB, M.Background: Preeclampsia (PE) prevalence is higher in women with chronic hypertension (CH). Predictive markers for PE in this group could be particularly important. We aimed to evaluate serum levels of PlGF and sFlt-1 throughout pregnancies complicated by isolated PE, CH and PE superimposed on CH (PE+CH) compared to normotensive (NT) pregnancies. Methods: Peripheral blood samples have been collected from NT and CH pregnancies at gestational ages of 20, 26, 32 and 36 weeks and stored to be assayed by ELISA. This study was approved by local Ethics Committee. To date, samples have been partially assayed. Results: Levels of sFlt-1 increased throughout pregnancy in all groups. Levels of PlGF increased until 32 weeks in pregnancies not complicated by PE (NT and CH) and until 26 weeks in those complicated by PE (PE and PE+HC), thereafter PlGF levels decreased in all groups. Conclusions: preliminary data, still too short for statistical testing.- EFFECT OF THE MICROENVIRONMENT ON THE PLACENTAL BEHAVIOUR: RESPONSE OF THE CHORIONIC VILLI TO NORMAL AND PREECLAMPTIC PREGNANT SERUM(2017) PRADO, Karen; CASTRO, Karla; LORENZON-OJEA, Aline; CARDOSO, Elaine; HOSHIDA, Mara; ALVES, Eliane; FRANCISCO, Rossana P. Vieira; ZUGAIB, Marcelo; BEVILACQUAL, Estela
conferenceObject IFN CHANGES PHAGOSOMAL MARKERS IN TROPHOBLAST CELLS(2014) HOSHIDA, Mara; AMARANTE-PAFFARO, Andrea; BEVILACQUA, Estela- Fetal-Maternal Hemorrhage in First-Trimester Intrauterine Hematoma(2021) NARCISO, Thaisa A. R. M.; HOSHIDA, Mara S.; COSTA, Priscilla R.; NIQUIRILO, Andrea; BIANCOLIN, Sckarlet E.; LIN, Lawrence H.; FRANCISCO, Rossana P. V.; BRIZOT, Maria L.Objective: The objective of this study was to compare the frequency and percentage of fetal hemoglobin (HbF%) by flow cytometry of (1) first-trimester asymptomatic patients with intrauterine hematoma (IUH), (2) first-trimester pregnant patients with vaginal bleeding (VB), and (3) first-trimester asymptomatic pregnant women without hematoma. Methods: Prospective study involving pregnant women in the first trimester of pregnancy. Patients with ultrasound findings of asymptomatic hematoma and with VB were paired with asymptomatic pregnant women of same gestational age without hematoma (control group [CG]). Maternal blood HbF% was evaluated by flow cytometry. The groups were compared in terms of circulating fetal hemoglobin and HbF%. Results: Sixty-six patients were selected, 22 with hematoma, 17 with bleeding, and 27 in the CG. Fetal hemoglobin was detected in 15 patients with hematoma (68.2%) and 13 with bleeding (76.5%) and in 20 of the control (74.1%) (p = 0.830). The mean HbF% of each group was 0.054, 0.012, and 0.042 for hematoma, bleeding, and control, respectively, and differences were not significant (p = 0.141). There was a moderate negative correlation between the volume of hematoma and HbF% (r(Spearman) = -0.527; p = 0.012). Conclusions: The fetal-maternal hemorrhage expressed by Hbf% in first-trimester pregnancies did not seem to differ between patients with and without ultrasound findings of IUH.
- EXPRESSION OF HLA-G, HLA-E AND HLA-F IN THE CHORIODECIDUAL INTERFACE OF COMPLETE HYDATIDIFORM MOLES(2019) LIN, Lawrence; TOSCANO, Marcello; HOSHIDA, Mara; SCHULTZ, Regina; FUSHIDA, Koji; FRANCISCO, Rossana
- The Impact of Immunosuppressive Drugs on Human Placental Explants(2019) GOMES, Sara Z.; ARAUJO, Franciele; BANDEIRA, Carla L.; OLIVEIRA, Leandro G.; HOSHIDA, Mara S.; ZUGAIB, Marcelo; FRANCISCO, Rossana P. V.; BEVILACQUA, EstelaThe use of immunosuppressive drugs guarantees the vitality of the graft and allows gestation in spite of intercurrences such as prematurity and intrauterine growth restriction. However, little is known about the direct effects of immunosuppressive drugs on placental cells. We investigated the effects of immunosuppressive drugs in the chorionic villous explants from human term placentas of healthy gestations. Human placental explants from term gestations (37-39 week gestational age, n = 12) were exposed to cyclosporine A (CSA, 0, 62.5, 125, 1250 ng/mL) or azathioprine (AZA, 0, 5, 10, 100 ng/mL) separately or, in combination for up to 48 hours. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed a significant decrease in the explant metabolic activity between AZA and the control group (24 hours, 100 ng/mL, 48 hours, all concentrations, P < .005). Cyclosporin A (CsA) reduced cell activity when associated with AZA (48 hours, P < .005). Fibrinoid deposits increased in AZA-treated explants alone (5 ng/mL, 48 hours; 10 ng/mL, 24-48 hours; P < .005) or when associated with CsA (10 AZA/125 CsA, P < .05), whereas in CsA treatment alone, there was an augment in syncytial knots (24-48 hours, P < .005). The sFLT1 gene (24 hours, P < .05) and protein (P < .005) expression increased in AZA and CsA-treatments separately or in combination (P < .05). Placental growth factor increased in AZA (24 hours, 10 ng/mL) and CsA (125 ng/mL; P < .05). In conclusion, our data indicate that AZA primarily acts on the villous metabolism, perturbing placental homeostasis. Since these drugs may alter the balance of angiogenic factors in its selection for clinical application, their impact on the behavior of placental villous should be considered.
conferenceObject Cross-talk between trophoblastic cells and maternal immune cells coordinated by Ccl25 Ccr9 during mouse embryo implantation(2013) WEINGRILL, R. B.; HOSHIDA, M. S.; MARTINHAGO, C. D.; BEVILACQUA, E.Background: CC’ and CXC’terminal chemokines are known to be involved with the polarity of blastocyst and uterus, leukocyte recruitment and capture/coordination of leukocyte migration. Particularly the chemokine CCL25 is expressed by the mouse trophoblast cells during implantation phase. Objectives: The aim of this study is to investigate the expression and localization of Ccl25 and its receptor Ccr9 during the mouse embryo implantation period. Methodology: Ccl25 and Ccr9 transcript abundance was measured by qPCR on fl ushed blastocyst from gestation days (gd) 3.5, 4.5, 5.5 and 7.5 and protein by immunofluorescence on frozen sections of implantation sites and on fl ushed blastocyst from gd4.5, 5.5 and 7.5. Results: Blastocyst expression of Ccl25/Ccr9 mRNA increased on gd4.5 and 5.5 and 7.5. Ccl25 was immunolocalized at trophoblast cells surrounding the blastocyst and glandular and uterine luminal epithelial cells, while Ccr9 was expressed at leukocytes inside maternal vessels surrounding the implanting embryo. Conclusion: Expression of chemokine /receptor during blastocyst implantation suggests a role and likely a cross-talk between trophoblast cells and the maternal immune cells and adds new elements to the understanding of trophoblast interactions during embryo implantation.- Ectoplacental Cone Isolation, Culture and Assessment(2014) BEVILACQUA, Estela; LORENZON, Aline R.; BANDEIRA, Carla L.; HOSHIDA, Mara S.; GARBELINI, Maria Cecília Da Lozzo; GONçALVES, Claudia ReginaDuring rodent development, a transient stage that is rapidly assembled in the extraembryonic placental compartment is the ectoplacental cone, which supports the first functional maternal–fetal interactions before completion of the placenta. During this period (gd 6–8.5), the ectoplacental cone plays a remarkable set of vital functions, finally contributing to cellular subtypes of placental development (to form the junctional zone: the secondary giant and spongiotrophoblast cells, in close association with the chorion involved in placental labyrinth differentiation). Specialized cells that arise from the ectoplacental cone, called secondary trophoblast giant cells (TGCs), are responsible for specific interactions with maternal tissues, which include displacement of uterine epithelium, invasion of the basement membrane and endometrial stroma, phagocytosis of maternal cells, opening of endometrial vessels, and replacement of endothelial cells. Association of TGCs with the maternal vessels guarantees a blood source that, having embedded the embryo, allows molecular exchange and embryonic development. In some rodent species, further invasion also occurs through the maternal vascular lumen. Along with a mechanical tissue involved with the lodging of the embryo into the uterine stroma, TGCs are actively synthetic cells that produce a large variety of regulatory molecules that act as endocrine, paracrine, and autocrine signals. TGCs are polyploidy, which is thought to accelerate differentiation and therefore increases the intensity and complexity of their roles. In this chapter, we describe and discuss the use of in vitro techniques that have been adapted for the study of the maternal–fetal interaction before placenta completion. Since the cells and the structure belonging to the ectoplacental cone are described in Chapter 10, we will focus here on the techniques used to establish TGCs in vitro by culturing blastocysts or taking ectoplacental explants. Identification of the cells and cocultures mimicking heterotypic cell–cell interactions in vivo are also described.
- THE IMMUNOSUPPRESSANT DRUG AZATHIOPRINE INDUCES EXPRESSION OF IL-18 IN PLACENTAL CELLS(2019) ARAUJO, Franciele; OLIVEIRA, Leandro; HOSHIDA, Mara; FRANCISCO, Rossana P. V.; MARINHO, Claudio; BEVILACQUA, Estela
- Bothrops jararaca Peptide with Anti-Hypertensive Action Normalizes Endothelium Dysfunction Involved in Physiopathology of Preeclampsia(2011) BENEDETTI, Gabriel; MORAIS, Katia L. P.; GUERREIRO, Juliano R.; OLIVEIRA, Eduardo Fontana de; HOSHIDA, Mara Sandra; OLIVEIRA, Leandro; SASS, Nelson; LEBRUN, Ivo; ULRICH, Henning; LAMEU, Claudiana; CAMARGO, Antonio Carlos Martins dePreeclampsia, a pregnancy-specific syndrome characterized by hypertension, proteinuria and edema, is a major cause of fetal and maternal morbidity and mortality especially in developing countries. Bj-PRO-10c, a proline-rich peptide isolated from Bothrops jararaca venom, has been attributed with potent anti-hypertensive effects. Recently, we have shown that Bj-PRO-10c-induced anti-hypertensive actions involved NO production in spontaneous hypertensive rats. Using in vitro studies we now show that Bj-PRO-10c was able to increase NO production in human umbilical vein endothelial cells from hypertensive pregnant women (HUVEC-PE) to levels observed in HUVEC of normotensive women. Moreover, in the presence of the peptide, eNOS expression as well as argininosuccinate synthase activity, the key rate-limiting enzyme of the citrulline-NO cycle, were enhanced. In addition, excessive superoxide production due to NO deficiency, one of the major deleterious effects of the disease, was inhibited by Bj-PRO-10c. Bj-PRO-10c induced intracellular calcium fluxes in both, HUVEC-PE and HUVEC, which, however, led to activation of eNOS expression only in HUVEC-PE. Since Bj-PRO-10c promoted biological effects in HUVEC from patients suffering from the disorder and not in normotensive pregnant women, we hypothesize that Bj-PRO-10c induces its anti-hypertensive effect in mothers with preeclampsia. Such properties may initiate the development of novel therapeutics for treating preeclampsia.
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