MARIA ANGELICA PERES
Projetos de Pesquisa
Unidades Organizacionais
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome(2013) SIMOES, Renata; FEITOSA, Weber Beringui; SIQUEIRA, Adriano Felipe Perez; NICHI, Marcilio; PAULA-LOPES, Fabiola Freitas; MARQUES, Mariana Groke; PERES, Maria Angelica; BARNABE, Valquiria Hyppolito; VISINTIN, Jose Antonio; ASSUMPCAO, Mayra Elena OrtizSperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.
conferenceObject Chronicle air pollution exposition and semen quality in hypercolesterolemic mice(2013) PERES, M. A.; CASTRO, K.; RIBEIRO-JUNIOR, G.; MENDES, C.; LOSANO, J.; VERAS, M.; SALDIVA, P.Air pollution (AP) is associated to lose of semen quality. It is also known that some population groups are more susceptible to atmospheric pollution effects due to a pre-existing health condition. This work detailed the effects AP in semen quality of mice knockout for low density lipoprotein receptor that represents an important risk group of human population. Animals were exposed to AP during pre and/or post natal period until adult life, with or without hypercholesterolemic diet. Semen analysis comprised sperm number and morphology, membrane, acrosome and DNA integrity, mitochondrial activity, oxidative stress and cytokines expression of seminal vesicle. The combination of the three factors (AP pre/post natal exposure and hypercholesterolemia) negatively impacted DNA fragmentation, mitochondrial activity, membrane integrity and oxidative stress. Other parameters did not show significant differences. Air pollution combined with hypercholesterolemia condition potentiated the negative effects on important parameters of semen leading male mice to present low fertility/infertility.conferenceObject HIGH-SALT INTAKE DURING PREGNANCY MODIFIES PLACENTA PHENOTYPE AT LEAST UNTIL THE THIRD GENERATION(2014) PERES, Maria A.; SOTO, Sonia F.; GUIMARADES, Carina F.; SILVA, Rafaela C. P.; OLIVEIRA, Ivone B. de; FURUKAWA, Luzia N. S.; HEIMANN, Joel C.conferenceObject Murine uterine receptivity markers are affected by particulate air pollution in a dose response manner(2013) CASTRO, K. R.; RIBEIRO-JUNIOR, R.; PERES, M.; SALDIVA, P.; MATSUDA, M.; VERAS, M.Air pollution (AP) is associated with increased rates of implantation failures in mice. The uterus requires a variety of factors to become receptive: cytokines (LIF), anti-adhesion (MUC-1) molecules and hormonal induced morphological changes. This study investigated the effects of chronic exposure to AP on uterine receptivity. Female mice were exposed to either filtered air or 2 different daily doses of AP. Estrous cycle, ovarian and uterine weight, number of corpora lutea (CL), uterine histopathology, LIF and MUC-1 expression were evaluated. Effects were dose dependent. Estrous cycle showed shorter duration with extended diestrus. Ovarian weights were increased without change in CL number. Histopathology indicated a decrease in volume and thickness of endometrium and increased diameter and thickness of glandular and luminal epithelium. MUC-1 expression was not altered but LIF was decreased during the implantation window. This suggests that AP exposure negatively affects endometrial receptivity altering the fine regulation of decidualization mediated by LIF expression.