Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

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art_SIMOES_Influence_of_bovine_sperm_DNA_fragmentation_and_oxidative_2013.PDF (224.41 KB)
Citações na Scopus
106
Tipo de produção
article
Data de publicação
2013
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
BIOSCIENTIFICA LTD
Autores
SIMOES, Renata
FEITOSA, Weber Beringui
SIQUEIRA, Adriano Felipe Perez
NICHI, Marcilio
PAULA-LOPES, Fabiola Freitas
MARQUES, Mariana Groke
BARNABE, Valquiria Hyppolito
VISINTIN, Jose Antonio
ASSUMPCAO, Mayra Elena Ortiz
Citação
REPRODUCTION, v.146, n.5, p.433-441, 2013
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.
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URI
https://observatorio.fm.usp.br/handle/OPI/4346
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Artigos e Materiais de Revistas Científicas - FM/Outros