MARIA ANGELICA PERES
Projetos de Pesquisa
Unidades Organizacionais
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
Agora exibindo 1 - 8 de 8
- Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome(2013) SIMOES, Renata; FEITOSA, Weber Beringui; SIQUEIRA, Adriano Felipe Perez; NICHI, Marcilio; PAULA-LOPES, Fabiola Freitas; MARQUES, Mariana Groke; PERES, Maria Angelica; BARNABE, Valquiria Hyppolito; VISINTIN, Jose Antonio; ASSUMPCAO, Mayra Elena OrtizSperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.
conferenceObject Chronicle air pollution exposition and semen quality in hypercolesterolemic mice(2013) PERES, M. A.; CASTRO, K.; RIBEIRO-JUNIOR, G.; MENDES, C.; LOSANO, J.; VERAS, M.; SALDIVA, P.Air pollution (AP) is associated to lose of semen quality. It is also known that some population groups are more susceptible to atmospheric pollution effects due to a pre-existing health condition. This work detailed the effects AP in semen quality of mice knockout for low density lipoprotein receptor that represents an important risk group of human population. Animals were exposed to AP during pre and/or post natal period until adult life, with or without hypercholesterolemic diet. Semen analysis comprised sperm number and morphology, membrane, acrosome and DNA integrity, mitochondrial activity, oxidative stress and cytokines expression of seminal vesicle. The combination of the three factors (AP pre/post natal exposure and hypercholesterolemia) negatively impacted DNA fragmentation, mitochondrial activity, membrane integrity and oxidative stress. Other parameters did not show significant differences. Air pollution combined with hypercholesterolemia condition potentiated the negative effects on important parameters of semen leading male mice to present low fertility/infertility.conferenceObject Salt intake during pregnancy influences offspring organ growth(2014) PERES, Maria; OLIVEIRA, Ivone de; FURUKAWA, Luzia; GUIMARAES, Carina; HEIMANN, JoelconferenceObject Transgenerational Effects of High Salt Diet During Gestation(2016) PERES, Maria Angelica; OLIVEIRA, Ivone Braga de; FURUKAWA, Luzia Naoko Shinohara; HEIMANN, JoelconferenceObject HIGH-SALT INTAKE DURING PREGNANCY MODIFIES PLACENTA PHENOTYPE AT LEAST UNTIL THE THIRD GENERATION(2014) PERES, Maria A.; SOTO, Sonia F.; GUIMARADES, Carina F.; SILVA, Rafaela C. P.; OLIVEIRA, Ivone B. de; FURUKAWA, Luzia N. S.; HEIMANN, Joel C.- Effect of maternal periodontitis on GLUT4 and inflammatory pathway in adult offspring(2019) MATTERA, Maria Sara de Lima Coutinho; CHIBA, Fernando Yamamoto; LOPES, Flavia Lombardi; TSOSURA, Thais Veronica Saori; PERES, Maria Angelica; BRITO, Victor Gustavo Balera; OLIVEIRA, Sandra Helena Penha de; PEREIRA, Renato Felipe; MARANI, Fernando; SANTOS, Rodrigo Martins dos; BELARDI, Bianca Elvira; TESSARIN, Gestter Willian Lattari; BENITES, Mariana Lopes; ERVOLINO, Edilson; HEIMANN, Joel Claudio; SUMIDA, Doris HissakoBackground Maternal periodontal disease leads to low birth weight (LBW), insulin resistance (IR), increased TNF-alpha levels, and alterations in insulin signaling in adult offspring. TNF-alpha has been associated with the stimulation of IKK beta/NF-kappa B, resulting in the decreased expression of GLUT4. Another mechanism that may be involved in decreasing GLUT4 expression is DNA methylation. This study aimed to evaluate in the adult offspring of rats with periodontal disease: IR, inflammatory pathways, DNA methylation, and expression of GLUT4. Methods Female Wistar rats were distributed into control and experimental periodontal disease groups. Seven days after induction of periodontal disease, both groups were mated with healthy male rats. After weaning, male offspring were distributed into control offspring (CN-o) and periodontal disease offspring (PED-o) groups. Body weights were measured from 0-75 days of age. At day 75, the following were measured in the offspring: IR (HOMA-IR index); TNF-alpha and NF-kappa Bp65 content in the gastrocnemius muscle (GM) by western blotting; IKK alpha/beta, JNK, ERK 1/2, NF-kappa Bp65, and NF-kappa Bp50 phosphorylation status in the GM by western blotting; DNA methylation by restriction digest and real-time PCR(qAMP); and expression of GLUT4 mRNA in the GM by real-time PCR. Results LBW, IR, increases in TNF-alpha, IKK alpha/beta, ERK 1/2, NF-kappa Bp65, and NF-kappa Bp50 decreased expression of GLUT4 mRNA were observed in the PED-o rats. No differences were identified in JNK phosphorylation status and DNA methylation in the evaluated regions of the GLUT4-encoding gene Slc2a4. Conclusion Maternal periodontal disease causes LBW, IR, activation of inflammatory pathways, and decreased GLUT4 expression in the GM of adult offspring.
conferenceObject Murine uterine receptivity markers are affected by particulate air pollution in a dose response manner(2013) CASTRO, K. R.; RIBEIRO-JUNIOR, R.; PERES, M.; SALDIVA, P.; MATSUDA, M.; VERAS, M.Air pollution (AP) is associated with increased rates of implantation failures in mice. The uterus requires a variety of factors to become receptive: cytokines (LIF), anti-adhesion (MUC-1) molecules and hormonal induced morphological changes. This study investigated the effects of chronic exposure to AP on uterine receptivity. Female mice were exposed to either filtered air or 2 different daily doses of AP. Estrous cycle, ovarian and uterine weight, number of corpora lutea (CL), uterine histopathology, LIF and MUC-1 expression were evaluated. Effects were dose dependent. Estrous cycle showed shorter duration with extended diestrus. Ovarian weights were increased without change in CL number. Histopathology indicated a decrease in volume and thickness of endometrium and increased diameter and thickness of glandular and luminal epithelium. MUC-1 expression was not altered but LIF was decreased during the implantation window. This suggests that AP exposure negatively affects endometrial receptivity altering the fine regulation of decidualization mediated by LIF expression.- COVID-19: Understanding the impact of anti-hypertensive drugs and hydroxychloroquine on the ACE1 and ACE2 in lung and adipose tissue in SHR and WKY rats(2023) CORREA, Beatriz Santos Geoffroy; BARROS, Silvana de; VAZ, Julia Braga; PERES, Maria Angelica; UCHIYAMA, Mayara Klimuk; SILVA, Alexandre Alves da; FURUKAWA, Luzia Naoko ShinoharaHypertensive individuals taking anti-hypertensive drugs from renin-angiotensin system inhibitors may exhibit a more severe evolution of the disease when contracting the SARS-CoV-2 virus (COVID-19 disease) due to potential increases in ACE2 expression. The study investigated ACE1 and ACE2 axes and hydroxychloroquine in the lungs and adipose tissue of male and female normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHRs). SHRs were treated with losartan (10 mg/kg/day) or captopril (10 mg/kg/day) for 14 days or 7 days with hydroxychloroquine (200 mg/kg/day) in drinking water. WKY rats were also treated for 7 days with hydroxychloroquine. Blood pressure (BP), protein, and mRNA expression of ACE1 and ACE2 were analyzed in serum, adipose, and lung tissues. Losartan and captopril reduced BP in both sexes in SHR, whereas hydroxychloroquine increased BP in WKY rats. Losartan reduced ACE2 in serum and lungs in both sexes and in adipose tissue of male SHRs. Captopril decreased ACE2 protein in the lung of females and in adipose tissue in both sexes of SHRs. Hydroxychloroquine decreased ACE1 and ACE2 proteins in the lungs in both sexes and adipose tissue in male SHRs. In female WKY rats, ACE2 protein was lower only in the lungs and adipose tissue. Losartan effectively inhibited ACE2 in male and captopril in female SHRs. Hydroxychloroquine inhibited ACE2 in male SHRs and female WKY rats. These results further our understanding of the ACE2 mechanism in patients under renin-angiotensin anti-hypertensive therapy and in many trials using hydroxychloroquine for COVID-19 treatment and potential sex differences in response to drug treatment.