ROGERIO PAZETTI
Projetos de Pesquisa
Unidades Organizacionais
LIM/61 - Laboratório de Pesquisa em Cirurgia Torácica, Hospital das Clínicas, Faculdade de Medicina
42 resultados
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article 5 Citação(ões) na Scopus An experimental rat model of ex vivo lung perfusion for the assessment of lungs after prostacyclin administration: inhaled versus parenteral routes(2011) CARDOSO, Paulo Francisco Guerreiro; PAZETTI, Rogerio; MORIYA, Henrique Takachi; PEGO-FERNANDES, Paulo Manuel; ALMEIDA, Francine Maria de; CORREIA, Aristides Tadeu; FECHINI, Karina; JATENE, Fabio BiscegliObjective: To present a model of prostaglandin I(2) (PGI(2)) administration (inhaled vs. parenteral) and to assess the functional performance of the lungs in an ex vivo lung perfusion system. Methods: Forty Wistar rats were anesthetized and placed on mechanical ventilation followed by median sterno-laparotomy and anticoagulation. The main pulmonary artery was cannulated. All animals were maintained on mechanical ventilation and were randomized into four groups (10 rats/group): inhaled saline (IS); parenteral saline (PS); inhaled PGI(2) (IPGI(2)); and parenteral PGI(2) (PPGI(2)). The dose of PGI(2) used in the IPGI(2) and PPGI(2) groups was 20 and 10 mu g/kg. respectively. The heart-lung blocks were submitted to antegrade perfusion with a low potassium and dextran solution via the pulmonary artery, followed by en bloc extraction and storage at 4 degrees C for 6 h. The heart-lung blocks were then ventilated and perfused in an ex vivo lung perfusion system for 50 min. Respiratory mechanics, hemodynamics, and gas exchange were assessed. Results: Mean pulmonary artery pressure following nebulization decreased in all groups (p < 0.001), with no significant differences among the groups. During the ex vivo perfusion, respiratory mechanics did not differ among the groups, although relative oxygenation capacity decreased significantly in the IS and PS groups (p = 0.04), whereas mean pulmonary artery pressure increased significantly in the IS group. Conclusions: The experimental model of inhaled PGI(2) administration during lung extraction is feasible and reliable. During reperfusion, hemodynamics and gas exchange trended toward better performance with the use of PGI(2) than that with the use of saline.- Tacrolimus impairs airway mucociliary clearance of rats(2024) SILVA, Maristela Prado E.; SOTO, Sonia de Fatima; ALMEIDA, Francine Maria de; CORREIA, Aristides Tadeu; PEGO-FERNANDES, Paulo Manuel; PAZETTI, RogerioObjectives: Tacrolimus (TAC) is the most widely used immunosuppressive agent after lung transplantation. Considering that the ciliary beat frequency (CBF) mainly depends on the cytoplasmic calcium concentration and that TAC can affect this due to its binding with the intracellular immunophilin FKBP12, we hypothesized that TAC could also impair the airway mucociliary clearance of rats. Methods: Sixty rats were divided into two groups (n = 30 each): Control = water; TAC = tacrolimus. After 7, 15 or 30 days of treatment, ten animals from each group were euthanized and the following parameters were studied: mucus transportability, CBF, mucociliary transport velocity (MCTV), and neutral and acid mucus production. Results: There was a significant decrease in CBF (Control vs TAC: 7 days, p = 0.008; 15 days, p = 0.007; 30 days, p = 0.001) and MCTV (Control vs TAC: 7 days, p = 0.004; 15 days, p < 0.001; 30 days, p < 0.001) in all immunosuppressed animals. TAC therapy also caused an increase in acid mucus production at all treatment times (Control vs TAC: 7 days, p = 0.001; 15 days, p = 0.043; 30 days, p = 0.001). Conclusions: TAC impairs airway mucociliary clearance of rats.
- An in vivo image acquisition system for the evaluation of tracheal mechanics in rats(2020) VALENGA, Marcelo Henrique; VITORASSO, Renato de Lima; RODRIGUES, Thiago Guimaraes; PAZETTI, Rogerio; CARDOSO, Paulo Francisco Guerreiro; MORIYA, Henrique Takachi; AOKI, Fabio GavaMechanical evaluation of tracheal grafts is of great relevance for transplant research. Although there are some publications demonstrating different techniques of tracheal mechanical evaluation, there is still no definitive or preferred protocol available. Here, we present a simple image processing acquisition system that can be used for in vivo experiments. Six male Wistar rats were submitted to orotracheal intubation and a longitudinal incision was made to expose the trachea. Images of tracheae were acquired from a video camera in different scenarios of bronchoconstriction using methacholine (MCh) (Basal, PBS, MCh 30 mu g/kg, MCh 300 mu g/kg, and postmetabolized) during imposed-inspiration and imposed-expiration. The area variation ratio (the ratio between areas during expiration vs. inspiration) was 1.1x for the Basal group, while the ratio for MCh 300 mu g/kg was 6.5x. The area variation of imaged tracheae was statistically significant at the dose of MCh 300 mu g/kg for imposed-inspiration versus imposed-expiration (P = .002). Likewise, elastance data of respiratory mechanics indicated a statistically significant difference at the dose of MCh 300 mu g/kg for imposed-inspiration versus imposed-expiration (P = .026). Our image processing analysis protocol presented corresponding behavior when compared to mechanical parameters of the respiratory system. In addition, our image acquisition system was able to highlight the differences between imposed-inspiration and imposed-expiration. Image analysis of the tracheal area variation seems to be in agreement with the elastance of the respiratory system. Taken together, these observations may help future studies of tracheal transplantation for in situ assessment of graft patency.
- Lodenafil treatment in the monocrotaline model of pulmonary hypertension in rats(2014) POLONIO, Igor Bastos; ACENCIO, Milena Marques Pagliareli; PAZETTI, Rogerio; ALMEIDA, Francine Maria de; SILVA, Brbara Soares da; PEREIRA, Karina Aparecida Bonifacio; SOUZA, RogerioWe assessed the effects of Iodenafil on hemodynamics and inflammation in the rat model of monocrotaline-induced pulmonary hypertension (PH). Thirty male Sprague-Dawley rats were randomly divided into three groups: control; monocrotaline (experimental model); and Iodenafila (experimental model followed by Iodenafil treatment, p.o., 5 mg/kg daily for 28 days) Mean pulmonary artery pressure (mPAP) was obtained by right heart catheterization. We investigated right ventricular hypertrophy (RVH) and IL-1 levels in lung fragments. The number of cases of RVH was significantly higher in the monocrotaline group than in the Iodenafil and control groups, as were mPAP and IL-1 levels. We conclude that Iodenafil can prevent monocrotaline-induced PH, RVH, and inflammation.
conferenceObject Side effects of tacrolimus upon airway epithelial tissue(2013) SILVA, Maristela Prado e; SOTO, Sonia; ALMEIDA, Francine; LIMONETE, Tatiana; PARRA, Edwin; PEGO-FERNANDES, Paulo; JATENE, Fabio; PAZETTI, Rogerio- Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats(2022) CORREIA, Aristides Tadeu; ALMEIDA, Francine Maria de; AUGUSTO-COTTET, Marcia Cristina; NOLASCO, Patricia; BENTO, Afonso Silva Alves; HIRANO, Hugo Kenji Matsushima; SOUZA, Maria Cecilia Ribeiro de; SANTOS, Elizabete Silva dos; CASTRO, Julia Helena Rodrigues de; MATSUDA, Monique; PEGO-FERNANDES, Paulo Manuel; PAZETTI, RogerioPrevious studies have shown that immunosuppressive drugs impair the airway mucociliary clearance of rats. However, considering the high specificity of basiliximab (BSX) and the absence of studies reporting its side effects, our aim was to investigate whether BSX, associated or not with triple therapy, impairs the mucociliary system. Forty rats were divided into 4 groups: Control, BSX, Triple, and BSX + Triple. After 15 days of treatment, animals were euthanized and the ciliary beating frequency (CBF), mucociliary transport velocity (MCTV), neutral and acid mucin production, Muc5ac and Muc5b gene expression, inflammatory cell number, and interleukin (IL)-6 concentration were analyzed. CBF and MCTV were lower in Triple and BSX + Triple groups (p < 0.05). Neutral mucin percentage was higher in Triple group (p < 0.05), and acid mucin percentage was higher in Triple and BSX + Triple groups (p < 0.05). The Muc5ac and Muc5b gene expression was higher in Triple and BSX + Triple groups (p < 0.05). Animals from Triple and BSX + Triple groups presented fewer mononuclear cells (p < 0.05). The number of polymorphonuclear cells was higher in the Triple group (p < 0.05). In the analysis of inflammatory cells in the blood, there was a decrease in lymphocytes and an increase in neutrophils in the Triple and BSX + Triple groups (p < 0.05). The concentration of IL-6 significantly increased in the animals of the Triple and BSX + Triple groups (p < 0.05). BSX did not change the mucociliary apparatus of rats.
conferenceObject Creatine Supplementation Attenuates Apoptosis and Proliferation of Inflammatory Cells in a Lung Transplantation Model(2018) ALMEIDA, F. M.; BATTOCHIO, A. S.; NAPOLI, J. P.; ALVES, K. A.; BALBIN, G. S.; OLIVEIRA-JUNIOR, M.; MORIYA, H. T.; PEGO-FERNANDES, P.; VIEIRA, R. P.; PAZETTI, R.- Effect of paclitaxel and methotrexate associated with cholesterol-rich nanoemulsions on ischemiareperfusion injury after unilateral lung transplantation in rats(2023) BATTOCHIO, Angela; TAVARES, Elaine; CORREIA, Aristides; ALMEIDA, Francine; CARVALHO, Priscila; GUIDO, Maria; PEGO-FERNANDES, Paulo; MARANHAO, Raul; PAZETTI, Rogerio
- Creatine Supply Attenuates Ischemia-Reperfusion Injury in Lung Transplantation in Rats(2020) ALMEIDA, Francine M.; BATTOCHIO, Angela S.; NAPOLI, Joao P.; ALVES, Katiusa A.; BALBIN, Grace S.; OLIVEIRA-JUNIOR, Manoel; MORIYA, Henrique T.; PEGO-FERNANDES, Paulo M.; VIEIRA, Rodolfo P.; PAZETTI, RogerioIschemia-reperfusion injury (IRI) is one of the factors limiting the success of lung transplantation (LTx). IRI increases death risk after transplantation through innate immune system activation and inflammation induction. Some studies have shown that creatine (Cr) protects tissues from ischemic damage by its antioxidant action. We evaluated the effects of Cr supplementation on IRI after unilateral LTx in rats. Sixty-four rats were divided into four groups: water + 90 min of ischemia; Cr + 90 min of ischemia; water + 180 min of ischemia; and Cr + 180 min of ischemia. Donor animals received oral Cr supplementation (0.5 g/kg/day) or vehicle (water) for five days prior to LTx. The left lung was exposed to cold ischemia for 90 or 180 min, followed by reperfusion for 2 h. We evaluated the ventilatory mechanics and inflammatory responses of the graft. Cr-treated animals showed a significant decrease in exhaled nitric oxide levels and inflammatory cells in blood, bronchoalveolar lavage fluid and lung tissue. Moreover, edema, cell proliferation and apoptosis in lung parenchyma were reduced in Cr groups. Finally, TLR-4, IL-6 and CINC-1 levels were lower in Cr-treated animals. We concluded that Cr caused a significant decrease in the majority of inflammation parameters evaluated and had a protective effect on the IRI after LTx in rats.
- Pulmonary emphysema induced by methylphenidate: experimental study(2015) RAPELLO, Gabriel Victor Guimaraes; ANTONIOLLI, Andreia; PEREIRA, Daniel Martins; FACCO, Gilberto; PEGO-FERNANDES, Paulo Manuel; PAZETTI, RogerioCONTEXT AND OBJECTIVE: Methylphenidate is the most widely used drug for treating attention deficit hyperactivity disorder. However, it has important side effects, such as abdominal pain, insomnia, anorexia and loss of appetite, and also some cases of early severe emphysema after drug abuse have been reported. Our aim was to investigate the development of pulmonary emphysema in rats that were subjected to different doses of methylphenidate. DESIGN AND SETTING: Experimental study carried out at the laboratory of a public university. METHODS: Eighteen male Wistar rats were divided into three groups: control (0.9% saline solution); MP 0.8 (methylphenidate, 0.8 mg/kg); MP 1.2 (methylphenidate, 1.2 mg/kg). After 90 days of daily gavage, the animals were sacrificed and lung tissue samples were prepared for analysis on the mean alveolar diameter (Lm). RESULTS: The Lm was greater in MP 0.8 (47.91 +/- 3.13; P < 0.01) and MP 1.2 (46.36 +/- 4.39; P < 0.05) than in the control group (40.00 +/- 3.48). CONCLUSION: Methylphenidate caused an increase in the alveolar diameter of rats, which was compatible with human pulmonary emphysema.