ELEAZAR CHAIB
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Gastroenterologia, Faculdade de Medicina - Docente
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado, Hospital das Clínicas, Faculdade de Medicina - Líder
77 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 77
- Translational Research for Pelvic Floor Transplantation(2017) GALVAO, Flavio; WAISBERG, Daniel; SEID, Victor; CRUZ, Ruy; TRALDI, Maria; ARAUJO, Bruno; PANTANALI, Carlos; ANDRAUS, Wellington; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz
conferenceObject A new Model of Hipothermic Pancreas Ischemia-Reperfusion in Rats(2013) SANTOS, Vinicius; FERRO, Oscar; PANTANALI, Carlos; ARANTES, Rubens; PECORA, Rafael; DAVID, Andre; CHAIB, Eleazar; D'ALBUQUERQUE, LuizconferenceObject Hyperacute Rejection in Multivisceral Xenotransplantation(2012) GALVAO, F.; SOLER, W.; POMPEU, E.; COSTA, A.; WAISBERG, D.; CAPELLOZI, V.; TEODORO, W.; VELOSA, A.; CHAIB, E.; D'ALBUQUERQUE, L.- Trisulfate Disaccharide Decreases Calcium Overload and Protects Liver Injury Secondary to Liver Ischemia/Reperfusion(2016) VASQUES, Enio Rodrigues; CUNHA, Jose Eduardo Monteiro; COELHO, Ana Maria Mendonca; SAMPIETRE, Sandra N.; PATZINA, Rosely Antunes; ABDO, Emilio Elias; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo Andrade; GODOY, Carlos M. G.; RODRIGUES, Tiago; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz A. C.Background Ischemia and reperfusion (I/R) causes tissue damage and intracellular calcium levels are a factor of cell death. Sodium calcium exchanger (NCX) regulates calcium extrusion and Trisulfated Disaccharide (TD) acts on NCX decreasing intracellular calcium through the inhibition of the exchange inhibitory peptide (XIP). Objectives The aims of this research are to evaluate TD effects in liver injury secondary to I/R in animals and in vitro action on cytosolic calcium of hepatocytes cultures under calcium overload. Methods Wistar rats submitted to partial liver ischemia were divided in groups: Control: (n = 10): surgical manipulation with no liver ischemia; Saline: (n = 15): rats receiving IV saline before reperfusion; and TD: (n = 15): rats receiving IV TD before reperfusion. Four hours after reperfusion, serum levels of AST, ALT, TNF-alpha, IL-6, and IL-10 were measured. Liver tissue samples were collected for mitochondrial function and malondialdehyde (MDA) content. Pulmonary vascular permeability and histologic parameters of liver were determined. TD effect on cytosolic calcium was evaluated in BRL3A hepatic rat cell cultures stimulated by thapsigargin pre and after treatment with TD. Results AST, ALT, cytokines, liver MDA, mitochondrial dysfunction and hepatic histologic injury scores were less in TD group when compared to Saline Group (p<0.05) with no differences in pulmonary vascular permeability. In culture cells, TD diminished the intracellular calcium raise and prevented the calcium increase pre and after treatment with thapsigargin, respectively. Conclusion TD decreases liver cell damage, preserves mitochondrial function and increases hepatic tolerance to I/R injury by calcium extrusion in Ca2+ overload situations.
conferenceObject Pelvic Floor Transplantation in The Reat.(2014) GALVAO, F.; BAPTISTA, R.; SEID, V.; WAISBERG, D.; RODRIGUES, P.; CHAIB, E.; D'ALBUQUERQUE, L.- A new heparin fragment decreases liver ischemia-reperfusion injury(2022) VASQUES, Enio R.; FIGUEIRA, Estela R. R.; ROCHA-FILHO, Joel A.; LANCHOTTE, Cinthia; XIMENES, Jorge L. S.; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo A.; RODRIGUES, Tiago; CUNHA, Jose E. M.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz A. C.; GALVAO, Flavio H. F.
- Gastric fundus submucosa as a site for islets transplantation: An experimental study(2018) MESQUITA, Gustavo Heluani Antunes de; JARDIM, Yuri Justi; IUAMOTO, Leandro Ryuchi; SUGUITA, Fabio Yuji; ESSU, Felipe Futema; OLIVEIRA, Lucas Torres; MEYER, Alberto; CRESCENZI, Alessandra; ROCHA-SANTOS, Vinicius; GALVAO, Flavio H. F.; ANDRAUS, Wellington; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto CarneiroBackground: Islets of Langerhans transplantation is a promising alternative for glycemic control in patients with type 1 diabetes. The graft site is a factor that has large impact on the functioning of this transplant, and the stomach appears to be a promising location. Our objective is to describe a new experimental model for the grafting of Islets of Langerhans in rat stomachs. Methodology: Islets of Langerhans were extracted from 45 isogenic male rats of the Lewis lineage and transplanted into 9 isogenic rats of the Wistar lineage; 5 in the gastric body submucosa, and 4 in the gastric fundus submucosa. Normoglycemia was defined as two successive measurements of < 250 mg/dL. No immunosuppression was used. The two groups glycemia control improvement were compared with t-student test. Results: The results obtained following the transplantation of the islets in 9 rats showed between 995 and 2310 islets transplanted (mean of 1367). The rats from the gastric submucosa group had a better glycemic level improvement, with a confidence equal to 83.94%. Conclusion: Islets graft into the gastric fundus submucosa is a viable model with potential for adequate glycemic control. This model gives potential for new perspectives and future studies in this area.
- Bile duct confluence: anatomic variations and its classification(2014) CHAIB, Eleazar; KANAS, Alexandre Fligelman; GALVAO, Flavio Henrique Ferreira; D'ALBUQUERQUE, Luiz Augusto CarneiroAccurate knowledge of the anatomy of the bile ducts is critical for successfully hepato-biliary surgery. We describe the anatomical variations of the confluence of the bile ducts, their branches patterns, frequency and classification. From 1996 to 2011, we have collected data of the bile duct confluence. 2,032 and 1,014 anatomical variations of right and left bile ducts, respectively, were reviewed and classified according to the branching pattern. The frequencies of each type of the right hepatic duct (RHD) were as follows: Type A1-1,247 (61.3 %); Type A2-296 (14.5 %); Type A3-272 (13.3 %); Type A4-124 (6.1 %); Type A5-21 (1 %) and others-72 (3.5 %) and, for the left hepatic duct (LHD) was as follows: Type B1-773 (76.2 %); Type B2-153 (15 %); Type B3-38 (3.7 %); Type B4-9 (0.8 %); Type B5-29 (2.8 %) and others-12 (1.1 %). Atypical branching patterns of both the right and left hepatic ducts were found in 14 and 8 %, respectively. The two most common variations of the RHD were right anterior and posterior hepatic ducts join together to form the RHD and trifurcation where the RHD is absent and right anterior and posterior hepatic ducts join directly to the confluence with the LHD to form the common hepatic duct. The two most common variations in the LHD were segment IV drainage to the left and right hepatic ducts.
- Does anti-hepatitis B virus vaccine make any difference in long-term number of liver transplantation?(2012) CHAIB, E.; COIMBRA, B. G. M. M.; GALVAO, F. H. F.; TATEBE, E. R.; SHINZATO, M. S.; D'ALBUQUERQUE, L. A. C.; MASSAD, E.Background: Hepatitis B virus (HBV) infection is a major cause of morbidity and mortality worldwide. Chronic hepatitis B infection is associated with an increased risk of cirrhosis, hepatic decompensation, and hepatocellular carcinoma. Our aim is to analyze, through a mathematical model, the potential impact of anti-HBV vaccine in the long-term (that is, decades after vaccination) number of LT. Methods: The model simulated that the prevalence of HBV infection was 0.5% and that approximately 20% of all the liver transplantation carried out in the state of Sao Paulo are due to HBV infection. Results: The theoretical model suggests that a vaccination program that would cover 80% of the target population would reach a maximum of about 14% reduction in the LT program. Conclusion: Increasing the vaccination coverage against HBV in the state of Sao Paulo would have a relatively low impact on the number of liver transplantation. In addition, this impact would take several decades to materialize due to the long incubation period of liver failure due to HBV.
- THE M-RNA, EXPRESSION OF SERCA2 AND NCX1 IN THE PROCESS OF PHARMACOLOGICAL CELL PROTECTION IN EXPERIMENTAL ACUTE PANCREATITIS INDUCED BY TAUROCHOLATE(2018) VASQUES, Enio Rodrigues; CUNHA, Jose Eduardo Monteiro; KUBRUSLY, Marcia Saldanha; COELHO, Ana Maria; SANPIETRI, Sandra N.; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo A.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto CarneiroBackground: Intracellular calcium overload is known to be a precipitating factor of pancreatic cell injury in acute pancreatitis (AP). Intracellular calcium homeostasis depends of Plasmatic Membrane Calcium ATPase (PMCA), Sarcoplasmic Endothelial Reticulum Calcium ATPase 2 (SERCA 2) and the Sodium Calcium Exchanger (NCX1). The antioxidant melatonin (Mel) and Trisulfate Disaccharide (TD) that accelerates NCX1 action could reduce the cell damage determined by the AP. Aim: To evaluate m-RNA expressions of SERCA2 and NCX1 in acute pancreatitis induced by sodium taurocholate in Wistar rats pre-treated with melatonin and/or TD. Methods: Wistar rats were divided in groups: 1) without AP; 2) AP without pre-treatment; 3) AP and Melatonin; 4) AP and TD; 5) AP and Melatonin associated to TD. Pancreatic tissue samples were collected for detection of SERCA2 and NCX1 m-R NA levels by polymerase chain reaction (PCR). Results: Increased m-RNA expression of SERCA2 in the melatonin treated group, without increase of m-RNA expression of the NCX1. The TD did not affect levels of SERCA2 and NCX1 m-RNA expressions. The combined melatonin and TD treatment reduced the m-RNA expression of SERCA2. Conclusions: The effect of melatonin is restricted to increased m-RNA expression of SERCA2. Although TD does not affect gene expression, its action in accelerating calcium exchanger function can explain the slightest expression of SERCA2 m-RNA when associated with Melatonin, perhaps by a joint action of drugs with different and but possibly complementary mechanisms.