KEILA KAZUE IDA
Projetos de Pesquisa
Unidades Organizacionais
LIM/08 - Laboratório de Anestesiologia, Hospital das Clínicas, Faculdade de Medicina
19 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 19
- Effect of Postoperative Xylazine Administration on Cardiopulmonary Function and Recovery Quality After Isoflurane Anesthesia in Horses(2013) IDA, Keila K.; FANTONI, Denise T.; IBIAPINA, Bruna T.; SOUTO, Maria-Teresa M. R.; ZOPPA, Andre L. V.; SILVA, Luis Claudio L. C.; AMBROSIO, Aline M.ObjectiveTo evaluate equine cardiopulmonary function and recovery quality after administration of 0.25 or 0.50mg/kg xylazine intravenously (IV) during recovery. Study DesignRandomized, blinded, prospective, clinical study. AnimalsHorses (n=20). MethodsDuring recovery after 3hours of isoflurane anesthesia for arthroscopic surgery, horses were administered either 0.25mg/kg (G25, n=10) or 0.50mg/kg (G50, n=10) xylazine intravenously. Vital signs and arterial blood samples were obtained during recovery before sedation (baseline), 5, 10, 20, 30, and 45minutes after xylazine and 30minutes after standing. The quality of recovery scores ranged from 10 to 72 (10=best, 72=worst). ResultsG25 horses recovered faster (meanSD, 33 +/- 5min) than G50 horses (50 +/- 7min, P<.0001). Mean maximal decrease in arterial oxygen tension was 55 +/- 11mmHg in G25 (at 10minutes; P<.05) and 54 +/- 7mmHg in G50 (at 20minutes; P<.01). G25 group had a total recovery score (23 [range 18-29]) and number of attempts to stand (4 +/- 2) greater than the G50 group (18 [10-23] and 1 +/- 1, respectively; P<.001). ConclusionsBoth doses of xylazine promoted a moderate and transient hypoxemia during recovery; however, the 0.5mg/kg dose produced a longer and improved quality of recovery from anesthesia.
conferenceObject RESUSCITATION WITH FLUID OR TERLIPRESSLN DOES NOT INFLUENCE COAGULATION STATUS IN A MODEL OF SEVERE CONTROLLED HAEMORRHAGIC SHOCK(2012) SASAKI, A. T. C.; OTSUKI, D. A.; IDA, K. K.; AULER JR., J. O. C.; MALBOUISSON, L. M. S.- Protection of cerebral microcirculation, mitochondrial function, and electrocortical activity by small-volume resuscitation with terlipressin in a rat model of haemorrhagic shock(2018) IDA, K. K.; CHISHOLM, K. I.; MALBOUISSON, L. M. S.; PAPKOVSKY, D. B.; DYSON, A.; SINGER, M.; DUCHEN, M. R.; SMITH, K. J.Background: During early treatment of haemorrhagic shock, cerebral perfusion pressure can be restored by small-volume resuscitation with vasopressors. Whether this therapy is improved with additional fluid remains unknown. We assessed the value of terlipressin and lactated Ringer's solution (LR) on early recovery of microcirculation, tissue oxygenation, and mitochondrial and electrophysiological function in the rat cerebral cortex. Methods: Animals treated with LR replacing three times (3LR) the volume bled (n = 26), terlipressin (n = 27), terlipressin plus 1LR (n = 26), 2LR (n = 16), or 3LR (n = 15) were compared with untreated (n = 36) and sham-operated rats (n = 17). In vivo confocal microscopy was used to assess cortical capillary perfusion, changes in tissue oxygen concentration, and mitochondrial membrane potential and redox state. Electrophysiological function was assessed by cortical somatosensory evoked potentials, spinal cord dorsum potential, and peripheral electromyography. Results: Compared with sham treatment, haemorrhagic shock reduced the mean (SD) area of perfused vessels [82% (SD 10%) vs 38% (12%); P<0.001] and impaired oxygen concentration, mitochondrial redox state [99% (4%) vs 59% (15%) of baseline; P<0.001], and somatosensory evoked potentials [97%(13%) vs 27%(19%) of baseline]. Administration of terlipressin plus 1LR or 2LR was able to recover these measures, but terlipressin plus 3LR or 3LR alone were not as effective. Spinal cord dorsum potential was preserved in all groups, but no therapy protected electromyographic function. Conclusions: Resuscitation from haemorrhagic shock using terlipressin with small-volume LR was superior to high-volume LR, with regard to cerebral microcirculation, and mitochondrial and electrophysiological functions.
- Ventilation distribution assessed with electrical impedance tomography and the influence of tidal volume, recruitment and positive end-expiratory pressure in isoflurane-anesthetized dogs(2017) AMBROSIO, Aline M.; CARVALHO-KAMAKURA, Tatiana P. A.; IDA, Keila K.; VARELA, Barbara; ANDRADE, Felipe S. R. M.; FACO, Lara L.; FANTONI, Denise T.Objective To examine the intrapulmonary gas distribution of low and high tidal volumes (V-T) and to investigate whether this is altered by an alveolar recruitment maneuver (ARM) and 5 cmH(2)O positive end-expiratory pressure (PEEP) during anesthesia. Study design Prospective randomized clinical study. Animals Fourteen client-owned bitches weighing 26 +/- 7 kg undergoing elective ovariohysterectomy. Methods Isoflurane-anesthetized dogs in dorsal recumbency were ventilated with 0 cmH(2)O PEEP and pressure-controlled ventilation by adjusting the peak inspiratory pressure (PIP) to achieve a low (7 mL kg(-1); n = 7) or a high (12 mL kg(-1); n = 7) V-T. Ninety minutes after induction (T90), an ARM (PIP 20 cmH(2)O for 10 seconds, twice with a 10 second interval) was performed followed by the application of 5 cmH(2)O PEEP for 35 minutes (RM35). The vertical (ventral = 0%; dorsal = 100%) and horizontal (right = 0%; left = 100%) center of ventilation (CoV), four regions of interest (ROI) (ventral, central-ventral, central-dorsal, dorsal) identified in electrical impedance tomography images, and cardiopulmonary data were analyzed using two-way repeated measures ANOVA. Results The low V-T was centered in more ventral (nondependent) areas compared with high V-T at T90 (CoV: 38.8 +/- 2.5% versus 44.6 +/- 7.2%; p = 0.0325). The ARM and PEEP shifted the CoV towards dorsal (dependent) areas only during high V-T (50.5 +/- 7.9% versus 41.1 +/- 2.8% during low V-T, p = 0.0108), which was more distributed to the central-dorsal ROI compared with low V-T (p = 0.0046). The horizontal CoV was centrally distributed and cardiovascular variables remained unchanged throughout regardless of the V-T, ARM, and PEEP. Conclusions and clinical relevance Both low and high V-T were poorly distributed to dorsal dependent regions, where ventilation was improved following the current ARM and PEEP only during high V-T. Studies on the role of high V-T on pulmonary complications are required.
- Effects of positive end-expiratory pressure titration on gas exchange, respiratory mechanics and hemodynamics in anesthetized horses (vol 40, pg 564, 2013)(2014) AMBROSIO, A. M.; IDA, K. K.; SOUTO, M. T.; OSHIRO, A. H.; FANTONI, D. T.
- Prevalence of Cognitive Impairment Without Dementia and Dementia in Tremembe, Brazil(2016) CESAR, Karolina G.; BRUCKI, Sonia M. D.; TAKADA, Leonel T.; NASCIMENTO, Luiz F. C.; GOMES, Camila M. S.; ALMEIDA, Milena C. S.; OLIVEIRA, Maira O.; PORTO, Fabio H. G.; SENAHA, Mirna L. H.; BAHIA, Valeria S.; SILVA, Thais B. L.; IANOF, Jessica N.; SPINDOLA, Livia; SCHMIDT, Magali T.; JORGE, Mario S.; VALE, Patricia H. F.; CECCHINI, Mario A.; CASSIMIRO, Luciana; SOARES, Roger T.; GONCALVES, Marcia R.; MARTINS, Ana C. S.; DARE, Patricia; SMID, Jerusa; PORTO, Claudia S.; CARTHERY-GOULART, Maria T.; YASSUDA, Monica S.; MANSUR, Leticia L.; NITRINI, RicardoBackground:The prevalence of cognitive impairment is insufficiently determined in developing countries. The aim of this study was to ascertain the prevalence of cognitive impairment without dementia and dementia in community-dwelling elderly in Brazil.Methods:This was a single-phase cross-sectional survey of the elderly (aged 60 years and above) living in the municipality of Tremembe, Brazil. Twenty percent of the households with elderly persons were randomly selected from urban and rural areas, to obtain a homogenous representation of all socioeconomic and cultural levels.Results:We assessed 630 individuals [mean age, 71.3 y (7.99); mean years of education, 4.9 (+/- 4.54)] and found prevalence rates of 17.5% (95% confidence interval, 14.6-20.6) for dementia and 19.5% (95% confidence interval, 16.6-22.8) for cognitive impairment without dementia. These prevalence rates were influenced by age (P<0.001) and by educational level (P<0.001). There was no significant sex difference among diagnostic groups (P=0.166). The prevalence of dementia was higher in relatively younger individuals (below 70 y) when compared with other studies. Besides, dementia was associated with low socioeconomic status, stroke, previous psychiatric disorder, alcoholism, and epilepsy.Conclusions:The prevalence of dementia in this study was higher than in other studies, particularly among younger elderly.
- Clinical monitoring of cardiac output assessed by transoesophageal echocardiography in anaesthetised dogs: a comparison with the thermodilution technique(2017) MANTOVANI, Matheus M.; FANTONI, Denise T.; GIMENES, Andre M.; CASTRO, Jacqueline R. de; FLOR, Patricia B.; IDA, Keila K.; SCHWARTZ, Denise S.Background: Cardiac output (CO) is an important haemodynamic parameter to monitor in patients during surgery. However, the majority of the techniques for measuring CO have a limited application in veterinary practice due to their invasive approach and associated complexity and risks. Transoesophageal echocardiography (TEE) is a technique used to monitor cardiac function in human patients during surgical procedures and allows CO to be measured non-invasively. This prospective clinical study aimed to compare the transoesophageal echocardiography using a transgastric view of the left ventricular outflow tract (LVOT) and the thermodilution (TD) technique for the assessment of CO during mean arterial pressure of 65-80 mmHg (normotension) and <65 mmHg (hypotension) in dogs undergoing elective surgery. Eight dogs were pre-medicated with acepromazine (0.05 mg/kg, IM), tramadol (4 mg/kg, IM) and atropine (0.03 mg/kg, IM), followed by anaesthetic induction with propofol (3-5 mg/kg IV) and maintenance with isoflurane associated with a continuous infusion rate of fentanyl (bolus of 3 mu g/kg followed by 0. 3 mu g/kg/min). The CO was measured by TEE (COTEE) and TD (COTD) at the end of expiration during normotension and hypotension (induced by isoflurane). Results: There was a strong positive correlation between COTEE and COTD (r = 0.925; P < 0.0001). The bias between COTD and COTEE was 0.14 +/- 0.29 L/min (limits of agreement, -0.44 to 0.72 L/min). The percentage error of CO measured by the two methods was 12.32%. In addition, a strong positive correlation was found between COTEE and COTD during normotension (r = 0.995; P < 0.0001) and hypotension (r = 0.78; P = 0.0223). Conclusions: The results of this study indicated that the transgastric view of the LVOT by TEE was a minimally invasive alternative to clinically monitoring CO in dogs during anaesthesia. However, during hypotension, the CO obtained by TEE was less reliable, although still acceptable.
- A comparison of the cardiopulmonary effects of pressure controlled ventilation and volume controlled ventilation in healthy anesthetized dogs(2016) FANTONI, Denise T.; IDA, Keila K.; LOPES, Thomas F. T.; OTSUKI, Denise A.; AULER JR., Jose Otavio C.; AMBROSIO, Aline M.Objective - To compare the effects of pressure controlled ventilation (PCV) with volume-controlled ventilation (VCV) on lung compliance, gas exchange, and hemodynamics in isoflurane-anesthetized dogs. Design - Prospective randomized study. Setting - Veterinary teaching hospital. Animals - Forty client-owned bitches undergoing elective ovariohysterectomy. Interventions - Dogs were randomly assigned to be ventilated with 100% oxygen using PCV (n = 20) or VCV (n = 20). The respiratory rate was 20/min and positive end-expiratory pressure (PEEP) was 5 cm H2O, with a tidal volume of 10 mL/kg. Cardiac output (CO) was measured using thermodilution. Cardiopulmonary and blood gas data were obtained during spontaneous ventilation and after 30 (T30) and 60 minutes (T60) of controlled ventilation. Measurements and Main Results - In dogs ventilated with PCV, at T30 and T60, PIP was lower (11.4 +/- 1.9 and 11.1 +/- 1.5 cm H2O, respectively) and static compliance (C-ST) was higher (51 +/- 7 and 56 +/- 6 mL/cm H2O, respectively) than in VCV group (PIP of 14.3 +/- 1.3 and 15.5 +/- 1.4 cm H2O; C-ST of 34 +/- 8 and 33 +/- 9 mL/cm H2O, P < 0.0001). Compared with spontaneous ventilation, both groups had decreased alveolar-arterial oxygen difference at T30 and T60 (PCV: 128 +/- 32 mm Hg vs 108 +/- 20 and 104 +/- 16 mm Hg, respectively; VCV: 131 +/- 38 mm Hg vs 109 +/- 19 and 107 +/- 14 mm Hg, respectively; P < 0.01), while CO was maintained at all time points. Conclusions - Compared to spontaneous ventilation, both ventilatory modes effectively improved gas exchange without hemodynamic impairment. PCV resulted in higher lung C-ST and lower PIP compared to VCV.
- In Vivo Imaging of Flavoprotein Fluorescence During Hypoxia Reveals the Importance of Direct Arterial Oxygen Supply to Cerebral Cortex Tissue(2016) CHISHOLM, K. I.; IDA, K. K.; DAVIES, A. L.; PAPKOVSKY, D. B.; SINGER, M.; DYSON, A.; TACHTSIDIS, I.; DUCHEN, M. R.; SMITH, K. J.Live imaging of mitochondrial function is crucial to understand the important role played by these organelles in a wide range of diseases. The mitochondrial redox potential is a particularly informative measure of mitochondrial function, and can be monitored using the endogenous green fluorescence of oxidized mitochondrial flavoproteins. Here, we have observed flavoprotein fluorescence in the exposed murine cerebral cortex in vivo using confocal imaging; the mitochondrial origin of the signal was confirmed using agents known to manipulate mitochondrial redox potential. The effects of cerebral oxygenation on flavoprotein fluorescence were determined by manipulating the inspired oxygen concentration. We report that flavoprotein fluorescence is sensitive to reductions in cortical oxygenation, such that reductions in inspired oxygen resulted in loss of flavoprotein fluorescence with the exception of a preserved 'halo' of signal in periarterial regions. The findings are consistent with reports that arteries play an important role in supplying oxygen directly to tissue in the cerebral cortex, maintaining mitochondrial function.
- Pharmacokinetics of intramuscular alfaxalone and its echocardiographic, cardiopulmonary and sedative effects in healthy dogs(2018) CRUZ-BENEDETTI, Inga-Catalina; BUBLOT, Isabelle; RIBAS, Thibault; FOUREL, Isabelle; VOGL, Claus; DUBOIS, Claire; MILANI, Mathilde; IDA, Keila Kazue; PORTIER, KarineThe pharmacokinetics and the effects of a single intramuscular (IM) dose of alfaxalone on sedation and cardiopulmonary and echocardiographic variables was studied in dogs. Twelve healthy adult Beagles (3 females, 9 males) were used in this prospective controlled cross-over trial. Echocardiography was performed with and without 4 mg kg(-1) alfaxalone IM with a week wash-out interval. Sedation (19-point scale; 0 = no sedation), cardiopulmonary parameters, blood gas analysis and plasma concentration of alfaxalone were assessed every 5 minutes following the injection (T0). The influence of the alfaxalone plasma concentration and time on physiological variables was tested using a linear model whereas echocardiographic measurements were compared between conscious and alfaxalone-administered dogs using paired t-tests. Compared to baseline, alfaxalone administration was followed by an increase in heart rate (HR) from T5 to T30 and a decrease in mean arterial pressure (MAP) at T10, T25 and T30, in stroke volume (SV; 15 +/- 5 to 11 +/- 3 ml; P<0.0001), and end-diastolic volume (EDV; 24.7 +/- 5.7 to 19.4 +/- 4.9 ml). Cardiac output (CO) and blood gas analysis did not change significantly throughout. Mean plasma half-life was 29 +/- 8 minutes, volume of distribution was 1.94 +/- 0.63 L kg(-1), and plasma clearance was 47.7 +/- 14.1 ml kg(-1) minute(-1). Moderate to deep sedation was observed from T5 to T35. Ten dogs showed paddling, trembling, nystagmus and strong reaction to sound during the procedure. Although there were no significant changes in CO and oxygenation, the impact of HR, MAP, SV, EDV alterations requires further investigations in dogs with cardiac disease.