ANA CRISTINA BREITHAUPT FALOPPA
Projetos de Pesquisa
Unidades Organizacionais
LIM/11 - Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação, Hospital das Clínicas, Faculdade de Medicina
71 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 71
- Formaldehyde inhalation reduces respiratory mechanics in a rat model with allergic lung inflammation by altering the nitric oxide/cyclooxygenase-derived products relationship(2013) LINO-DOS-SANTOS-FRANCO, Adriana; GIMENES-JUNIOR, Joao Antonio; LIGEIRO-DE-OLIVEIRA, Ana Paula; BREITHAUPT-FALOPPA, Ana Cristina; ACCETURI, Beatriz Golega; VITORETTI, Luana Beatriz; MACHADO, Isabel Daufenback; OLIVEIRA-FILHO, Ricardo Martins; FARSKY, Sandra Helena Poliselli; MORIYA, Henrique Takachi; TAVARES-DE-LIMA, WothanBronchial hyperresponsiveness is a hallmark of asthma and many factors modulate bronchoconstriction episodes. A potential correlation of formaldehyde (FA) inhalation and asthma has been observed; however, the exact role of FA remains controversial. We investigated the effects of FA inhalation on Ovalbumin (OVA) sensitisation using a parameter of respiratory mechanics. The involvement of nitric oxide (NO) and cyclooxygenase-derived products were also evaluated. The rats were submitted, or not, to FA inhalation (1%, 90 min/day, 3 days) and were OVA-sensitised and challenged 14 days later. Our data showed that previous FA exposure in allergic rats reduced bronchial responsiveness, respiratory resistance (Rrs) and elastance (Ers) to methacholine. FA exposure in allergic rats also increased the iNOS gene expression and reduced COX-1. L-NAME treatment exacerbated the bronchial hyporesponsiveness and did not modify the Ers and Rrs, while Indomethacin partially reversed all of the parameters studied. The L-NAME and Indomethacin treatments reduced leukotriene B-4 levels while they increased thromboxane B-2 and prostaglandin E-2. In conclusion, FA exposure prior to OVA sensitisation reduces the respiratory mechanics and the interaction of NO and PGE(2) may be representing a compensatory mechanism in order to protect the lung from bronchoconstriction effects.
conferenceObject FEMALE RATS PRESENT HIGHER LUNG INFLAMMATION AFTER BRAIN DEATH FOLLOWED BY EX VIVO PERFUSION(2021) RICARDO-DA-SILVA, Fernanda Yamamoto; ARMSTRONG- JR., Roberto; OTTENS, Petra; ZANDEN, Judith van; VIDAL-DOS-SANTOS, Marina; MOREIRA, Luiz Felipe Pinho; ERASMUS, Michiel; LEUVENINK, Henri; BREITHAUPT-FALOPPA, Ana Cristina- Equity, Diversity and Inclusion (EDI) in Organ Transplantation: An ESOT Survey About EDI Within ESOT as an Organization and its Educational Activities, and Transplantation Research and Science(2023) PENGEL, L. H. M.; KAISAR, M.; BENJAMENS, S.; IBRAHIM, M.; RICCI, V.; BELLINI, M. I.; BREITHAUPT-FALOPPA, A. C.; FALK, C.; MAPLE, H.; MARSON, L.; ORTIZ, F.; PAPALOIS, V.; PAREDES, D.; FORSBERG, A.The European Society of Organ Transplantation (ESOT) strives to promote equity, diversity, and inclusion (EDI) across all its activities. We surveyed the transplant community's experiences and perspectives regarding EDI within ESOT as an organization and its educational activities, and research in general. A total of 299 respondents completed the questionnaire. About half agreed that ESOT's Executive Committee, Council, and Sections/Committees are diverse and inclusive (51%) and that ESOT promotes EDI in its live and digital educational activities (54%). Forty percent of respondents agreed that scientific and clinical trials in the field of transplantation are diverse and inclusive. Despite the wide distribution of the survey, most of the respondents self-identified as White and were either physician or surgeon. However, the results contribute a unique insight into the experiences and perspectives of the transplantation community regarding EDI. Whilst ESOT is committed to the principles of EDI, perceptions and the high number of proposals show the apparent need to prioritize efforts to embed EDI across ESOT and transplantation science. These data should constitute a starting point for change and provide guidance for future efforts to promote EDI within the transplantation community.
article Nephropathy induced by renal microembolism: a characterization of biochemical and histopathological changes in rats(2019) BERSANI-AMADO, Luiz Eduardo; ROCHA, Bruno Ambrsio da; SCHNEIDER, Larissa Carla Lauer; AMES, Franciele Queiroz; FALOPPA, Ana Cristina Breithaupt; ARAUJO, Gabriela Bataglini; DANTAS, Jailson Araujo; BERSANI-AMADO, Ciomar Aparecida; CUMAN, Roberto Kenji NakamuraThe aim of this study was to investigate some biochemical parameters of renal function and the vascular, glomerular, tubular, and interstitial manifestations in the progression of nephropathy induced by renal microembolism. Renal microembolism was induced by the arterial injection of polymethacrylate microspheres in the remnant kidney of nephrectomized rats. Animals 110-120 days old were randomly divided into three groups: the control group (C; normal), the nephrectomized group (S; nephrectomized that did not undergo renal microembolism), and the model group (M, nephrectomized animals that underwent renal arterial microembolism). The animals were evaluated 30, 60, and 90 days after the induction of a renal microembolism. Blood and urine samples were collected to determine serum creatinine (Cr) and urea (Ur) concentrations and urine total protein (Pt) concentrations. The kidneys were weighed and processed for histopathological analysis using hematoxylin and eosin (HE), periodic acid-Schiff (PAS), Mallory-Azan, and Picro-Sirius staining. The samples were also subjected to immunohistochemistry with a proliferating cell nuclear antigen (PCNA) and a vascular endothelial growth factor receptor (VEGFR). The data demonstrated evidence of the occurrence of vascular, glomerular, tubular, and interstitial abnormalities in the renal tissue, and changes in the biochemical parameters of renal function (serum Cr and Ur and of 24-h urine Pt) in this experimental model of nephropathy induced by renal microembolism, which may indicate the development of chronic kidney disease (CKD). Additionally, the findings indicate that this is a good reproducibility model that may be useful for studying the pathogenesis of CKD that is caused by atheroembolism and possible treatment alternatives.- Long-term lung inflammation is reduced by estradiol treatment in brain dead female rats(2021) RICARDO-DA-SILVA, Fernanda Yamamoto; ARMSTRONG-JR, Roberto; VIDAL-DOS-SANTOS, Marina; CORREIA, Cristiano de Jesus; SILVA, Raphael dos Santos Coutinho e; ANUNCIACAO, Lucas Ferreira da; MOREIRA, Luiz Felipe Pinho; LEUVENINK, Henri Gerrit Derk; BREITHAUPT-FALOPPA, Ana CristinaOBJECTIVES: Lung transplantation is limited by the systemic repercussions of brain death (BD). Studies have shown the potential protective role of 17 beta-estradiol on the lungs. Here, we aimed to investigate the effect of estradiol on the long-lasting lung inflammatory state to understand a possible therapeutic application in lung donors with BD. METHODS: Female Wistar rats were separated into 3 groups: BD, subjected to brain death (6h); E2-T0, treated with 17 beta-estradiol (50 mu g/mL, 2 mL/h) immediately after brain death; and E2-T3, treated with 17 beta-estradiol (50 mu g/ml, 2 ml/h) after 3h of BD. Complement system activity and macrophage presence were analyzed. TNF-alpha, IL-1 beta, IL-10, and IL-6 gene expression (RT-PCR) and levels in 24h lung culture medium were quantified. Finally, analysis of caspase-3 gene and protein expression in the lung was performed. RESULTS: Estradiol reduced complement C3 protein and gene expression. The presence of lung macrophages was not modified by estradiol, but the release of inflammatory mediators was reduced and TNF-alpha and IL-1 beta gene expression were reduced in the E2-T3 group. In addition, caspase-3 protein expression was reduced by estradiol in the same group. CONCLUSIONS: Brain death-induced lung inflammation in females is modulated by estradiol treatment. Study data suggest that estradiol can control the inflammatory response by modulating the release of mediators after brain death in the long term. These results strengthen the idea of estradiol as a therapy for donor lungs and improving transplant outcomes.
- Simvastatin protects against intestinal ischemia/reperfusion-induced pulmonary artery dysfunction(2022) PERES, Emilia C.; VICTORIO, Jamaira A.; NUNES-SOUZA, Valeria; BREITHAUPT-FALOPPA, Ana Cristina; RABELO, Luiza A.; TAVARES-DE-LIMA, Wothan; DAVEL, Ana Paula; ROSSONI, Luciana V.Aims: The lung is an important target organ damage in intestinal ischemia/reperfusion (II/R), but mechanisms involved in II/R-induced pulmonary artery (PA) dysfunction, as well as its treatment, are not clear. The present study aimed to investigate the mechanisms involved in the II/R-induced PA dysfunction and a possible protective role of acute simvastatin pretreatment.Main methods: Male Wistar rats were subjected to occlusion of the superior mesenteric artery for 45 min followed by 2 h reperfusion (II/R) or sham-operated surgery (sham). In some rats, simvastatin (20 mg/kg, oral gavage) was administrated 1 h before II/R. Key findings: II/R reduced acetylcholine-induced relaxation and phenylephrine-induced contraction of PA seg-ments, which were prevented by acute simvastatin pretreatment in vivo or restored by inducible nitric oxide synthase (iNOS) inhibition in situ with 1400 W. Elevated reactive oxygen species (ROS) levels and higher nuclear translocation of nuclear factor kappa B (NF Kappa B) subunit p65 were observed in PA of II/R rats and prevented by simvastatin. Moreover, simvastatin increased superoxide dismutase (SOD) activity and endothelial nitric oxide synthase (eNOS) expression in PA of the II/R group as well as prevented the increased levels of interleukin (IL)-1 beta and IL-6 in lung explants following II/R.Significance: The study suggests that pretreatment with a single dose of simvastatin prevents the II/R-induced increase of inflammatory factors and oxidative stress, as well as PA endothelial dysfunction and adrenergic hyporreactivity. Therefore, acute simvastatin administration could be therapeutic for pulmonary vascular disease in patients suffering from intestinal ischemic events.
- Hydroxymethylnitrofurazone lymphatic uptake with nanostructured lipid carrier after oral administration in rats(2024) SOUZA, Aline de; SCARIM, Caue Benito; COTRIM, Paulo Cesar; BARBOSA JUNIOR, Fernando; ROCHA, Bruno Alves; CALIXTO, Leandro Augusto; CORREIA, Cristiano Jesus; ARAUJO, Gabriel Lima de Barros; LOBENBERG, Raimar; BOU-CHACRA, Nadia Araci; BREITHAUPT-FALOPPA, Ana CristinaBackground: Leishmaniasis, caused by the protozoan Leishmania sp., infects phagocyte cells present in lymphatic organs. This study demonstrates the influence of nanostructured lipid carrier-loaded hydroxymethylnitrofurazone (NLC-NFOH) on lymphatic uptake using a chylomicron-blocking flow model in rats. Method: Lymphatic uptake of NFOH was assessed 1 h after oral administration of dimethyl sulfoxide with NFOH or NLC-NFOH with and without cycloheximide pretreatment. Result: Dimethyl sulfoxide with NFOH and NLC-NFOH showed NFOH serum concentrations of 0.0316 and 0.0291 mu g/ml, respectively. After chylomicron blocking, NFOH was not detected. Conclusion: Despite log P below 5, NFOH was successfully taken up by the lymphatic system. Long-chain fatty acids and particle size might be main factors in these findings. NLC-NFOH is a promising and convenient platform for treating leishmaniasis via oral administration.
- Development of an HPLC Method for Identification and Quantification of Anti-leishmaniasis Drug Candidate NFOH After Oral Administration of NLC-NFOH in Rats(2023) SOUZA, Aline de; ALMEIDA, Maria Karilly Lima de; BARBOSA, Joao Antonio; YUKUYAMA, Megumi Nishitani; CORREIA, Cristiano Jesus; BREITHAUPT-FALOPPA, Ana Cristina; BOU-CHACRA, Nadia Araci; CALIXTO, Leandro AugustoHydroxymethylnitrofurazone (NFOH) is a prodrug synthesized from the original nitrofurazone compound (NF). NFOH has shown activity in vivo against Trypanosoma cruzi and in vitro activity against Leishmania amazonensis. However, it has shown less toxicity than NF. This study aims to develop and evaluate the selectivity, carryover, matrix effect, linearity, precision and accuracy, of a bioanalytical method using HPLC to quantify NFOH and NF. The defined method conditions were mobile phase acetonitrile: water (20:80 v/v), Zorbax SB-C18, 5 mu m (4.6 x 250 mm) flow rate of 1.2 mL min(-1), at UV detection of 370 nm. The linearity obtained for NFOH and NF was in the range of 0.025-3.0 mu g mL(-1) with a correlation coefficient > 0.98. The precision (relative standard deviation) was 2.44-13.77% and 2.61-18.42% for NFOH and NF, respectively; the accuracy was 2.66-14.28% and 2.09-19.06% for NFOH and NF, respectively. In conclusion this method was successfully developed to determine and quantify NFOH and NF in serum after oral administration of nanostructured lipid carrier with NFOH. It opens opportunities to be applied in in vivo studies in novel NFOH nanoparticles formulations.
conferenceObject Estradiol Modulation of Brain Death Effects on Heart Tissue in Female Rats(2018) ARMSTRONG JUNIOR, R.; RICARDO-DA-SILVA, F. Y.; BASILIO, L. J. L.; VIDAL, M. S.; SANNOMIYA, P.; MOREIRA, L. F. P.; BREITHAUPT-FALOPPA, A. C.conferenceObject SEX-DIFFERENCES ON PLATELET AGGREGATION AND MICROVASCULAR PERFUSION AFTER BRAIN DEATH IN RATS(2019) CORREIA, Cristiano de Jesus; BREITHAUPT-FALOPPA, Ana Cristina; SILVA, Raphael dos Santos Coutinho e; SANTOS, Marina Vidal dos; ANUNCIACAO, Lucas Ferreira da; LEUVENINK, Hendrik Gerrit Derk; MOREIRA, Luiz Felipe Pinho