JOYCE TIYEKO KAWAKAMI

(Fonte: Lattes)
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Lattes
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Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina

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Agora exibindo 1 - 10 de 24
  • article 1 Citação(ões) na Scopus
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    Infectious Agents Related with Collagen Degradation and Inflammation in Myxomatous Mitral Valve Degeneration
    (2016) SR., Marcos G. Tiveron; SR., Pablo M. A. Pomerantzeff; HIGUCHI, Maria L.; REIS, Marcia; PEREIRA, Jaqueline; KAWAKAMI, Joyce; IKEGAMI, Renata; SR., Carlos M. Brandao; SR., Fabio B. Jatene
  • article 58 Citação(ões) na Scopus
    Mitochondrial Swelling and Incipient Outer Membrane Rupture in Preapoptotic and Apoptotic Cells
    (2012) SESSO, A.; BELIZARIO, J. E.; MARQUES, M. M.; HIGUCHI, M. L.; SCHUMACHER, R. I.; COLQUHOUN, A.; ITO, E.; KAWAKAMI, J.
    Outer mitochondrial membrane (OMM) rupture was first noted in isolated mitochondria in which the inner mitochondrial membrane (IMM) had lost its selective permeability. This phenomenon referred to as mitochondrial permeability transition (MPT) refers to a permeabilized inner membrane that originates a large swelling in the mitochondrial matrix, which distends the outer membrane until it ruptures. Here, we have expanded previous electron microscopic observations that in apoptotic cells, OMM rupture is not caused by a membrane stretching promoted by a markedly swollen matrix. It is shown that the widths of the ruptured regions of the OMM vary from 6 to 250 nm. Independent of the perforation size, herniation of the mitochondrial matrix appeared to have resulted in pushing the IMM through the perforation. A large, long focal herniation of the mitochondrial matrix, covered with the IMM, was associated with a rupture of the OMM that was as small as 6 nm. Contextually, the collapse of the selective permeability of the IMM may precede or follow the release of the mitochondrial proteins of the intermembrane space into the cytoplasm. When the MPT is a late event, exit of the intermembrane space proteins to the cytoplasm is unimpeded and occurs through channels that transverse the outer membrane, because so far, the inner membrane is impermeable. No channel within the outer membrane can expose to the cytoplasm a permeable inner membrane, because it would serve as a conduit for local herniation of the mitochondrial matrix. Anat Rec, 2012. (c) 2012 Wiley Periodicals, Inc.
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    SERUM ACTIVE COLLAGENASE FROM PATHOGENIC ARCHAEAL COLLAGENASE MAY CAUSE HEART FAILURE IN CHAGASIC PATIENTS
    (2019) HIGUCHI, Maria De Lourdes; KAWAKAMI, Joyce; IKEGAMI, Renata; REIS, Marcia; MORENO, Camila R.; PEREIRA, Jaqueline; IANNI, Barbara; BUCK, Paula; SANTOS, Marilia; BOCCHI, Edimar
  • article 0 Citação(ões) na Scopus
    Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes
    (2021) PEREIRA, Jaqueline de Jesus; IKEGAMI, Renata Nishiyama; KAWAKAMI, Joyce Tiyeko; GARAVELO, Sherrira Menezes; REIS, Marcia Martins; PALOMINO, Suely Aparecida Pinheiro; MANGINI, Sandrigo; MORENO, Camila Rodrigues; BARROS, Samar Freschi de; SOUZA, Aline Rodrigues; HIGUCHI, Maria de Lourdes
    BackgroundIdiopathic dilated cardiomyopathy (IDCM) myocardial inflammation may be associated with external triggering factors such as infectious agents. Here, we searched if moderate/severe heart transplantation rejection is related to the presence of myocardial inflammation in IDCM explanted hearts, associated with microbial communities. MethodReceptor myocardial samples from 18 explanted hearts were separated into groups according to post-transplant outcome: persistent moderate rejection (PMR; n = 6), moderate rejection (MR; n = 7) that regressed after pulse therapy, and no rejection (NR; n = 5)/light intensity rejection. Inflammation was quantified through immunohistochemistry (IHC), and infectious agents were evaluated by IHC, molecular biology, in situ hybridization technique, and transmission electron microscopy (TEM). ResultsNR presented lower numbers of macrophages, as well as B cells (p = 0.0001), and higher HLA class II expression (p <= 0.0001). PMR and MR showed higher levels of Mycoplasma pneumoniae (p = 0.003) and hepatitis B core (p = 0.0009) antigens. NR presented higher levels of parvovirus B19 (PVB19) and human herpes virus 6 (HHV6) and a positive correlation between Borrelia burgdorferi (Bb) and enterovirus genes. Molecular biology demonstrated the presence of M. pneumoniae, Bb, HHV6, and PVB19 genes in all studied groups. TEM revealed structures compatible with the cited microorganisms. ConclusionsThis initial study investigating on infectious agents and inflammation in the IDCM explanted hearts showed that the association between M. pneumoniae and hepatitis B core was associated with a worse outcome after HT, represented by MR and PMR, suggesting that different IDCM microbial communities may be contributing to post-transplant myocardial rejection.
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    Lack of Protective Exosomes to Remove AMZ1 Archaeal Collagenase is Associated With Heart Failure in Chagas' Disease
    (2017) HIGUCHI, Maria de Lourdes; KAWAKAMI, Joyce T.; SANTOS, Marilia H.; IKEGAMI, Renata N.; REIS, Marcia M.; IANNI, Barbara; BUCK, Paula; BOCCHI, Edimar
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    Serum Microvesicles Containing Archaeal DNA as a New Pathogenetic Factor for Heart Failure in Chagas' Disease
    (2018) HIGUCHI, Maria de Lourdes; KAWAKAMI, Joyce T.; REIS, Marcia M.; OLIVEIRA, Luanda; PEREIRA, Jaqueline J.; IANNI, Barbara; BUCK, Paula; BOCCHI, Edimar A.
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    Fatal acute myocardial infarction related to lack of exosomes and low removal of free Mycoplasma pneumoniae lipoproteins
    (2017) HIGUCHI, M. L.; SANTOS, M. H. H.; IKEGAMI, R. N.; REIS, M. M.; KAWAKAMI, J. T.; PEREIRA, J. J.; PALOMINO, S. A. P.; BENSENOR, I. J. M.; LOTUFO, P. A.
  • article 2 Citação(ões) na Scopus
    Infectious agents is a risk factor for myxomatous mitral valve degeneration: A case control study
    (2017) TIVERON, Marcos Gradim; POMERANTZEFF, Pablo Maria Alberto; HIGUCHI, Maria de Lourdes; REIS, Marcia Martins; PEREIRA, Jaqueline de Jesus; KAWAKAMI, Joyce Tieko; IKEGAMI, Renata Nishiyama; BRANDAO, Carlos Manuel de Almeida; JATENE, Fabio Biscegli
    Background: The etiology of myxomatous mitral valve degeneration (MVD) is not fully understood and may depend on time or environmental factors for which the interaction of infectious agents has not been documented. The purpose of the study is to analyze the effect of Mycoplasma pneumoniae (Mp), Chlamydophila pneumoniae (Cp) and Borrelia burgdorferi (Bb) on myxomatous mitral valve degeneration pathogenesis and establish whether increased in inflammation and collagen degradation in myxomatous mitral valve degeneration etiopathogenesis. Methods: An immunohistochemical test was performed to detect the inflammatory cells (CD20, CD45, CD68) and Mp, Bb and MMP9 antigens in two groups. The in situ hybridization was performed to detect Chlamydophila pneumoniae and the bacteria study was performed using transmission electron microscopy. Group 1 (n = 20), surgical specimen composed by myxomatous mitral valve degeneration, and group 2 (n = 20), autopsy specimen composed by normal mitral valve. The data were analyzed using SigmaStat version 20 (SPSS Inc., Chicago, IL, USA). The groups were compared using Student's t test, Mann-Whitney test. A correlation analysis was performed using Spearman's correlation test. P values lower than 0.05 were considered statistically significant. Results: By immunohistochemistry, there was a higher inflammatory cells/mm2 for CD20 and CD45 in group 1, and CD68 in group 2. Higher number of Mp and Cp antigens was observed in group 1 and more Bb antigens was detected in group 2. The group 1 exhibited a positive correlation between the Bb and MVD percentage, between CD45 and Mp, and between MMP9 with Mp. These correlations were not observed in the group 2. Electron microscopy revealed the presence of structures compatible with microorganisms that feature Borrelia and Mycoplasma characteristics. Conclusions: The presence of infectious agents, inflammatory cells and collagenases in mitral valves appear to contribute to the pathogenesis of MVD. Mycoplasma pneumoniae was strongly related with myxomatous mitral valve degeneration. Despite of low percentage of Borrelia burgdorferi in MD group, this agent was correlated with myxomatous degeneration and this may occour due synergistic actions between these infectious agents likely contribute to collagen degradation.
  • article 6 Citação(ões) na Scopus
    Prolonged presence of replication-competent SARS-CoV-2 in mildly symptomatic individuals: A report of two cases
    (2021) CORREA, Maria C. Mendes; LEAL, Fabio E.; BOAS, Lucy S. Villas; WITKIN, Steven S.; PAULA, Anderson de; MENDONZA, Tania R. Tozetto; FERREIRA, Noely E.; CURTY, Gislaine; CARVALHO, Pedro S. de; BUSS, Lewis F.; COSTA, Silvia F.; CARVALHO, Flavia M. da Cunha; KAWAKAMI, Joyce; TANIWAKI, Noemi N.; PAIAO, Heuder; BIZARIO, Joao C. da Silva; JESUS, Jaqueline G. de; SABINO, Ester C.; ROMANO, Camila M.; GREPAN, Regina M. Z.; SESSO, Antonio
    It has been estimated that individuals with COVID-19 can shed replication-competent virus up to a maximum of 20 days after initiation of symptoms. The majority of studies that addressed this situation involved hospitalized individuals and those with severe disease. Studies to address the possible presence of SARS-CoV-2 during the different phases of COVID-19 disease in mildly infected individuals, and utilization of viral culture techniques to identify replication-competent viruses, have been limited. This report describes two patients with mild forms of the disease who shed replication-competent virus for 24 and 37 days, respectively, after symptom onset.