RODRIGO MELIM ZERBINATI
Projetos de Pesquisa
Unidades Organizacionais
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
Agora exibindo 1 - 8 de 8
- Noninvasive Techniques for Management of Erythema Multiforme(2023) MARTINS, Fabiana; PALLOS, Debora; CANDEIA, Jodkandlys; ZERBINATI, Rodrigo; BRAZ-SILVA, Paulo Henrique; CAMPOS, LuanaAn 18-year-old man was referred for a diagnosis of extensive oral lesions. During the interview, he reported a medical history of ganglionic tuberculosis, type 2 herpes infection, and significant weight loss due to dysphagia. Intraoral exam revealed multiple painful and ulcerated lesions covered by pseudomembrane. Lesions were observed on the labial and buccal mucosa, tongue, and soft palate. The laboratory findings included serum positivity for the Epstein-Barr virus, and salivary tests showed positive values for herpes simplex virus (HSV-2) and human herpesvirus (HHV-7). The diagnostic hypothesis was based on clinical findings and viral infection detected in the saliva, which triggered an immunological disorder, that is, erythema multiforme (EM). The treatment consisted of antimicrobial photodynamic therapy (aPDT), with substantial improvement in pain and healing as seen in the following twenty-four hours. Complete resolution of the lesions was achieved five days after the first session. Once the diagnosis of virus-induced EM was confirmed, noninvasive techniques (e.g., salivary tests and aPDT) were very successful and can be indicated for managing these lesions.
- Lack of direct association between oral mucosal lesions and SARS-CoV-2 in a cohort of patients hospitalised with COVID-19(2022) SCHWAB, Gabriela; PALMIERI, Michelle; ZERBINATI, Rodrigo M.; SARMENTO, Dmitry J. S.; REIS, Thais; ORTEGA, Karem L.; KANO, Italo T.; V, Rafael A. Caixeta; HASSEUS, Bengt; SAPKOTA, Dipak; JUNGES, Roger; GIANNECCHINI, Simone; COSTA, Andre L. F.; JALES, Sumatra M. C. P.; LINDOSO, Jose A. L.; GALLO, Camila Barros; BRAZ-SILVA, Paulo H.Background COVID-19 is a disease affecting various human organs and systems, in which the virus seeks to interact with angiotensin-converting enzyme 2 receptors. These receptors are present in the oral cavity, but the direct relationship between such an interaction and possible oral manifestations of COVID-19 is still unclear. Aim The present study evaluated oral manifestations in a cohort of COVID-19 patients during the period of hospitalisation. Methods In total, 154 patients presenting moderate-to-severe forms of COVID-19 had their oral mucosa examined twice a week until the final outcome, either discharge or death. The oral alterations observed in the patients were grouped into Group 1 (pre-existing conditions and opportunistic oral lesions) and Group 2 (oral mucosal changes related to hospitalization). Results Oral lesions found in the patients of Group 1 are not suggestive of SARS-CoV-2 infection as they are mainly caused by opportunistic infections. On the other hand, oral alterations found in the patients of Group 2 were statistically (P < 0.001) related to intubation and longer period of hospitalisation. Conclusion It is unlikely that ulcerative lesions in the oral cavity are a direct manifestation of SARS-CoV-2 or a marker of COVID-19 progression.
- Quantification of torque teno virus (TTV) DNA in saliva and plasma samples in patients at short time before and after kidney transplantation(2022) BATISTA, Alexandre Mendes; CAETANO, Matheus W.; STINCARELLI, Maria A.; MAMANA, Ana C.; ZERBINATI, Rodrigo Melim; SARMENTO, Dmitry J. S.; GALLOTTINI, Marina; V, Rafael A. Caixeta; MEDINA-PESTANA, Jose; HASSEUS, Bengt; ZANELLA, Louise; TOZETTO-MENDOZA, Tania R.; GIANNECCHINI, Simone; BRAZ-SILVA, Paulo H.Background Several reports have proposed that the viral load of torque teno virus (TTV) in plasma is a biomarker of immune function in solid organ transplantation (SOT) and in allogeneic hematopoietic stem cell transplantation. Additionally, for the latter one, TTV-DNA quantification in saliva has also been suggested. Aim to investigate the correlation between the TTV viral load and immune function in paired saliva and plasma samples in patients on kidney transplantation. Materials and Methods TTV-DNA viral load was quantified in paired samples of saliva and plasma from 71 patients before and a short-time after renal-transplantation by real-time PCR. Results The data obtained from 213 paired samples showed a slight consistency in the comparison between saliva and plasma, with prevalence of TTV-DNA being 58%, 52% and 60% in saliva samples and 60%, 73% and 90% in plasma samples before and at 15-20 and 45-60 days after transplantation, respectively. Additionally, a high TTV viral load was observed in plasma at 15-20 and 45-60 days after transplantation compared to that observed in saliva at the same time. Conclusions Overall, monitoring TTV-DNA in saliva samples could be an additional fast non-invasive option to assess the immune functionality in SOT populations.
- SARS-CoV-2 in saliva, viremia and seroprevalence for COVID-19 surveillance at a single hematopoietic stem cell transplantation center: a prospective cohort study(2022) MOBILE, Rafael Zancan; WARNAWIN, Stephanie von Stein Cubas; KOJO, Teresinha Keiko; RODRIGUES, Jessica Alline Pereira; CAVILHA, Adriana Mendes de Quadros; ZERBINATI, Rodrigo Melim; ADAMOSKI, Douglas; OLIVEIRA, Jaqueline Carvalho de; CONZENTINO, Marcelo Santos; HUERGO, Luciano Fernandes; GRADIA, Daniela Fiori; BRAZ-SILVA, Paulo Henrique; SCHUSSEL, Juliana LucenaThis prospective cohort study aims to analyze the surveillance of COVID-19 at a single hematopoietic stem cell transplantation (HSCT) center in Brazil, in 29 patients undergoing allogeneic HSCT and 57 healthcare workers (nurses and dentists), through viral shedding of SARS-CoV-2 in saliva and plasma and seroprevalence of anti-SARS-CoV-2 IgG. In addition, we report two cases with prolonged persistent detection of SARS-CoV-2 without seroconversion. The sample collection was performed seven times for patients and five times for healthcare workers. Only two patients tested positive for SARS-CoV-2 in their saliva and plasma samples (6.9%) without seroconversion. All healthcare workers were asymptomatic and none tested positive. Two patients (6.9%) and four nurses (8%) had positive serology. No dentists had positive viral detection or positive serology. Our results reflect a low prevalence of positive RT-PCR and seroprevalence of SARS-CoV-2 in patients and healthcare workers at a single HSCT center. Results have also corroborated how the rigorous protocols adopted in transplant centers were even more strengthened in this pandemic scenario.
- Use of saliva and RT-PCR screening for SARS-CoV-2 variants of concern: Surveillance and monitoring(2022) ZERBINATI, Rodrigo Melim; PALMIERI, Michelle; SCHWAB, Gabriela; FELIX, Alvina Clara; MARTINHO, Herculano; GIANNECCHINI, Simone; TO, Kelvin Kai-Wang; LINDOSO, Jose Angelo Lauletta; ROMANO, Camila Malta; BRAZ-SILVA, Paulo HenriqueGenomic surveillance has been applied since the beginning of the COVID-19 pandemic to track the spread of the virus, leading to the characterization of multiple SARS-CoV-2 variants, including variants of concern (VOC). Although sequencing is the standard method, a rapid molecular test for screening and surveillance of VOC is considered for detection. Furthermore, using alternative saliva as specimen collection facilitates the implementation of a less invasive, self-collected sample. In this study, we applied a combinatory strategy of saliva collection and reverse transcription polymerase chain reaction (RT-PCR) for SARS-CoV-2 VOC detection. Saliva samples from patients attending a tertiary hospital with suspected COVID-19 were collected and SARS-CoV-2 RNA was detected using SARS-CoV-2 RT-qPCR reagent kit (PerkinElmer). Positive saliva samples were screened for SARS-CoV-2 VOC with previously described RT-PCR for Alpha, Beta, and Gamma variants. Saliva samples were positive in 171 (53%) of 324 tested. A total of 108 (74%) from positive samples were also positive for VOC by RT-PCR screening. Those samples were found between January and August 2021. This approach allowed us to successfully use an alternative and complementary tool to genomic surveillance to monitor the circulation of SARS-CoV-2 VOC in the studied population.
- MALDI-TOF mass spectrometry of saliva samples as a prognostic tool for COVID-19(2022) LAZARI, Lucas C.; ZERBINATI, Rodrigo M.; ROSA-FERNANDES, Livia; SANTIAGO, Veronica Feijoli; ROSA, Klaise F.; ANGELI, Claudia B.; SCHWAB, Gabriela; PALMIERI, Michelle; SARMENTO, Dmity J. S.; MARINHO, Claudio R. F.; ALMEIDA, Janete Dias; TO, Kelvin; GIANNECCHINI, Simone; WRENGER, Carsten; SABINO, Ester C.; MARTINHO, Herculano; LINDOSO, Jose A. L.; DURIGON, Edison L.; BRAZ-SILVA, Paulo H.; PALMISANO, GiuseppeBackground The SARS-CoV-2 infections are still imposing a great public health challenge despite the recent developments in vaccines and therapy. Searching for diagnostic and prognostic methods that are fast, low-cost and accurate are essential for disease control and patient recovery. The MALDI-TOF mass spectrometry technique is rapid, low cost and accurate when compared to other MS methods, thus its use is already reported in the literature for various applications, including microorganism identification, diagnosis and prognosis of diseases. Methods Here we developed a prognostic method for COVID-19 using the proteomic profile of saliva samples submitted to MALDI-TOF and machine learning algorithms to train models for COVID-19 severity assessment. Results We achieved an accuracy of 88.5%, specificity of 85% and sensitivity of 91.5% for classification between mild/moderate and severe conditions. When we tested the model performance in an independent dataset, we achieved an accuracy, sensitivity and specificity of 67.18, 52.17 and 75.60% respectively. Conclusion Saliva is already reported to have high inter-sample variation; however, our results demonstrates that this approach has the potential to be a prognostic method for COVID-19. Additionally, the technology used is already available in several clinics, facilitating the implementation of the method. Further investigation using a larger dataset is necessary to consolidate the technique.
- The Differential Clinical Impact of Human Coronavirus Species in Children With Cystic Fibrosis(2012) SILVA FILHO, Luiz Vicente Ribeiro Ferreira da; ZERBINATI, Rodrigo Melim; TATENO, Adriana Fumie; BOAS, Lucy Vilas; ALMEIDA, Marina Buarque de; LEVI, Jose Eduardo; DREXLER, Jan Felix; DROSTEN, Christian; PANNUTI, Claudio SergioWe investigated the clinical impact of human coronaviruses (HCoV) OC43, 229E, HKU1 and NL63 in pediatric patients with cystic fibrosis (CF) during routine and exacerbation visits. A total of 408 nasopharyngeal aspirate samples were obtained from 103 patients over a 1-year period. Samples positive for HCoV were submitted for nucleotide sequencing to determine the species. Nineteen samples (4.65%) were positive for HCoV, of which 8 were positive for NL63, 6 for OC43, 4 for HKU1, and 1 for 229E. Identification of HCoV was not associated with an increased rate of respiratory exacerbations, but NL63-positive patients had higher exacerbation rates than patients who were positive for other HCoV species.
- Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients(2022) BANDEIRA, Carla Carolina Silva; MADUREIRA, Karen Cristina Rolim; ROSSI, Meire Bocoli; GALLO, Juliana Failde; SILVA, Ana Paula Marques Aguirra da; TORRES, Vilanilse Lopes; LIMA, Vinicius Alves de; KESPER JUNIOR, Norival; ALMEIDA, Janete Dias; ZERBINATI, Rodrigo Melim; BRAZ-SILVA, Paulo Henrique; LINDOSO, Jose Angelo Lauletta; MARTINHO, Herculano da SilvaIt has been reported that patients diagnosed with COVID-19 become critically ill primarily around the time of activation of the adaptive immune response. However the role of antibodies in the worsening of disease is not obvious. Higher titers of anti-spike immunoglobulin IgG1 associated with low fucosylation of the antibody Fc tail have been associated to excessive inflammatory response. In contrast it has been also reported that NP-, S-, RBD- specific IgA, IgG, and IgM are not associated with SARS-CoV-2 viral load, indicating that there is no obvious correlation between antibody response and viral antigen detection. In the present work the micro-Fourier-transform infrared reflectance spectroscopy (micro-FTIR) was employed to investigate blood serum samples of healthy and COVID-19-ill (mild or oligosymptomatic) individuals (82 healthcare workers volunteers in ""Instituto de Infectologia Emilio Ribas"", Sao Paulo, Brazil). The molecular-level-sensitive, multiplexing quantitative and qualitative FTIR data probed on 1 mu 1_ of dried biofluid was compared to signal-to-cutoff index of chemiluminescent immunoassays CLIA and ELISA (IgG antibodies against SARS-CoV-2). Our main result indicated that 1702-1785 cm(-1) spectral window (carbonyl C=O vibration) is a spectral marker of the degree of IgG glycosylation, allowing to probe distinctive sub-populations of COVID-19 patients, depending on their degree of severity. The specificity was 87.5 %while the detection rate of true positive was 100%. The computed area under the receiver operating curve was equivalent to CLIA, ELISA and other ATR-FTIR methods (> 0.85). In summary, overall discrimination of healthy and COVID-19 individuals and severity prediction as well could be potentially implemented using micro-FTIR reflectance spectroscopy on blood serum samples. Considering the minimal and reagent-free sample preparation procedures combined to fast (few minutes) outcome of FTIR we can state that this technology is suitable for fast screening of immune response of individuals with COVID-19. It would be an important tool in prospective studies, helping investigate the physiology of the asymptomatic, oligosymptomatic, or severe individuals and measure the extension of infection dissemination in patients.