CAMILA RENNO GUIMARAES
Projetos de Pesquisa
Unidades Organizacionais
LIM/36 - Laboratório de Pediatria Clínica, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
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conferenceObject Phenotypic differences in leukocyte populations among septic and healthy preterm and full-term newborns(2012) QUINELLO, C.; SILVEIRA-LESSA, A. L.; RENNO, C.; CIANCIARULLO, M. A.; REDONDO, A. C. C.; CECCON, M. E. J. R.; CARNEIRO-SAMPAIO, M.; PALMEIRA, P.- Hydrophobicity and antioxidant activity acting together for the beneficial health properties of nordihydroguaiaretic acid(2015) PARACATU, Luana Chiquetto; FARIA, Carolina Maria Quinello Gomes de; ZERAIK, Maria Luiza; QUINELLO, Camila; RENNO, Camila; PALMEIRA, Patrcia; FONSECA, Luiz Marcos da; XIMENES, Valdecir FariasNordihydroguaiaretic acid (NDGA) and rosmarinic acid (RA), phenolic compounds found in various plants and functional foods, have known antioxidant and anti-inflammatory properties. In the present study, we comparatively investigated the importance of hydrophobicity and oxidisability of NDGA and RA, regarding their antioxidant and pharmacological activities. Using a panel of cell-free antioxidant protocols, including electrochemical measurements, we demonstrated that the anti-radical capacities of RA and NDGA were similar. However, the relative capacity of NDGA as an inhibitor of NADPH oxidase (ex vivo assays) was significantly higher compared to RA. The inhibitory effect on NADPH oxidase was not related to simple scavengers of superoxide anions, as confirmed by oxygen consumption by the activated neutrophils. The higher hydrophobicity of NDGA was also a determinant for the higher efficacy of NDGA regarding the inhibition of the release of hypochlorous acid by PMA-activated neutrophil and cytokine (TNF-alpha and IL-10) production by Staphylococcus aureus-stimulated peripheral blood mononuclear cells. In conclusion, although there have been extensive studies about the pharmacological properties of NDGA, our study showed, for the first time, the importance not only of its antioxidant activity, but also its hydrophobicity as a crucial factor for pharmacological action.
- Caffeic Acid Phenethyl Ester: Consequences of Its Hydrophobicity in the Oxidative Functions and Cytokine Release by Leukocytes(2014) PARACATU, Luana Chiquetto; FARIA, Carolina Maria Quinello Gomes; QUINELLO, Camila; RENNO, Camila; PALMEIRA, Patricia; ZERAIK, Maria Luiza; FONSECA, Luiz Marcos da; XIMENES, Valdecir FariasNumerous anti-inflammatory properties have been attributed to caffeic acid phenethyl ester (CAPE), an active component of propolis. NADPH oxidases are multienzymatic complexes involved in many inflammatory diseases. Here, we studied the importance of the CAPE hydrophobicity on cell-free antioxidant capacity, inhibition of the NADPH oxidase and hypochlorous acid production, and release of TNF-alpha and IL-10 by activated leukocytes. The comparison was made with the related, but less hydrophobic, caffeic and chlorogenic acids. Cell-free studies such as superoxide anion scavenging assay, triene degradation, and anodic peak potential (E-pa) measurements showed that the alterations in the hydrophobicity did not provoke significant changes in the oxidation potential and antiradical potency of the tested compounds. However, only CAPE was able to inhibit the production of superoxide anion by activated leukocytes. The inhibition of the NADPH oxidase resulted in the blockage of production of hypochlorous acid. Similarly, CAPE was the more effective inhibitor of the release of TNF-alpha and IL-10 by Staphylococcus aureus stimulated cells. In conclusion, the presence of the catechol moiety and the higher hydrophobicity were essential for the biological effects. Considering the involvement of NADPH oxidases in the genesis and progression of inflammatory diseases, CAPE should be considered as a promising anti-inflammatory drug.
conferenceObject Phagocytosis of Escherichia coli and Staphylococcus aureus by monocytes and neutrophils from septic and healthy preterm and term newborns(2012) QUINELLO, C.; SILVEIRA-LESSA, A. L.; RENNO, C.; CIANCIARULLO, M. A.; REDONDO, A. C. C.; CECCON, M. E. J. R.; CARNEIRO-SAMPAIO, M.; PALMEIRA, P.- Healthy Preterm Newborns Show an Increased Frequency of CD4(+)CD25(high)CD127(low)FOXP3(+) Regulatory T Cells with a Naive Phenotype and High Expression of Gut-Homing Receptors(2016) RENNO, C.; NADAF, M. I. V.; ZAGO, C. A.; CARNEIRO-SAMPAIO, M.; PALMEIRA, P.Treg cells are crucial to prevent immune dysregulation, but little is known about the frequency of these cells in neonates, particularly in very/moderate and late preterm newborns studied as separate groups. The CD4(+)CD25(hi)CD127(lo)FOXP3(+) Treg population was phenotypically characterized to assess maturation markers and gut-homing integrins by flow cytometry in the cord blood of healthy preterm newborns born at 30-33(6/7) gestation weeks (Group 1), at 34-36(6/7) gestation weeks (Group 2) and term newborns born at 37-41 gestation weeks (Group 3), compared to healthy adults. An inverse correlation of the Treg percentage and gestational age was found, with significantly higher frequencies in Group 1 compared to Groups 2 and 3 and in Group 2 compared to Group 3, and significantly higher Treg frequencies and numbers in the neonates compared to the adults. All of the newborns exhibited increased Treg frequencies with a naive phenotype compared to adults. Cytotoxic T-lymphocyte-associated protein 4 CTLA-4 expression in the naive Treg was decreased in both preterm groups compared with those from term newborns and adults, and in the memory Treg from Group 1 compared with the other groups. The frequencies of Treg expressing alpha 4 beta 7 and alpha 4 beta 1 integrins were higher in both preterm groups, but significantly different only in Group 1, when compared with those from the term newborns and the adults. In conclusion, although a high frequency of Treg is present in newborns, an immature phenotype with a higher expression of CD45RA and alpha 4 beta 7/alpha 4 beta 1 and a lower expression of CTLA-4 is found, particularly in the very preterm group.