DEBORAH DE CAMARGO HIZUME
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- Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice (vol 8, 12636, 2018)(2018) GENARO, Isabella Santos de; ALMEIDA, Francine Maria de; HIZUME-KUNZLER, Deborah Camargo; MORIYA, Henrique Takachi; SILVA, Ronaldo Aparecido; CRUZ, Joao Carlos Goncalves; LOPES, Renan Boeira; RIGHETTI, Renato Fraga; VIEIRA, Rodolfo de Paula; SAIKI, Mitiko; MARTINS, Milton Arruda; TIBERIO, Iolanda de Fatima Lopes Calvo; ARANTES-COSTA, Fernanda Magalhaes; SARAIVA-ROMANHOLO, Beatriz Mangueira
conferenceObject Effects of exercise on lung inflammation in ovalbumin-sensitized and single challenged mice(2012) AVILA, Leonardo Cesar Melo; FORTKAMP, Barbara; BRUGGEMANN, Thayse Regina; SILVA, Morgana Duarte da; PERINI, Adenir; SANTOS, Adair Roberto Soares; HIZUME, Deborah de Camargo- Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice(2018) GENARO, Isabella Santos de; ALMEIDA, Francine Maria de; HIZUME-KUNZLER, Deborah Camargo; MORIYA, Henrique Takachi; SILVA, Ronaldo Aparecido; CRUZ, Joao Carlos Goncalves; LOPES, Renan Boeira; RIGHETTI, Renato Fraga; VIEIRA, Rodolfo de Paula; SAIKI, Mitiko; MARTINS, Milton Arruda; TIBERIO, Iolanda de Fatima Lopes Calvo; ARANTES-COSTA, Fernanda Magalhaes; SARAIVA-ROMANHOLO, Beatriz MangueiraWork-exacerbated asthma (WEA) is defined as preexisting asthma that worsens with exposure to irritants [e.g., chlorine (Cl-2) derivatives] in the workplace. The maximum allowable concentration in the workplace of Cl-2 exposure is 3 mg/m(3) (described in OSHA). We investigated in an experimental asthma model in mice the effects of a single exposure to a sodium hypochlorite dose with this allowed chlorine concentration and a tenfold higher dose. Acute chlorine exposure at 3.3 mg/m(3) in the OVA-sensitized group increased eosinophils in the peribronquial infiltrate, cytokine production, nasal mucus production and the number of iNOS positive cells in the distal lung compared to only sensitized mice. The exposure to a higher dose of 33.3 mg/m(3) in the OVA-sensitized group resulted in an increase in respiratory system elastance, in the total and differential numbers of inflammatory cells in bronchoalveolar lavage fluid, IL-4, IL-5, and IL-17 in the lungs, eosinophils in peribronquial infiltrate and mucus content in nasal compared to non-exposed and sensitized animals. In this asthma model, chorine exposures at an allowable dose, contributed to the potentiation of Th2 responses. The functional alterations were associated with increased iNOS and ROCK-2 activation in the distal lung.
conferenceObject Effects Of High-Intensity Exercise On Inflammation And Oxidative Stress In Mice With Chronic Allergic Pulmonary Inflammation(2013) BRUGGEMANN, T. R.; AVILA, L. M.; FORTKAMP, B.; GREIFFO, F. R.; SILVA, M. D.; BOBINSKI, F.; MARTINS, L. M.; MARTINS, D. F.; SANTOS, A. S.; HIZUME, D. C.- Effects of High-Intensity Swimming on Lung Inflammation and Oxidative Stress in a Murine Model of DEP-Induced Injury(2015) AVILA, Leonardo C. M.; BRUGGEMANN, Thayse R.; BOBINSKI, Franciane; SILVA, Morgana Duarte da; OLIVEIRA, Regiane Carvalho; MARTINS, Daniel Fernandes; MAZZARDO-MARTINS, Leidiane; DUARTE, Marta Maria Medeiros Frescura; SOUZA, Luiz Felipe de; DAFRE, Alcir; VIEIRA, Rodolfo de Paula; SANTOS, Adair Roberto Soares; BONORINO, Kelly Cattelan; KUNZLER, Deborah de C. HizumeStudies have reported that exposure to diesel exhaust particles (DEPs) induces lung inflammation and increases oxidative stress, and both effects are susceptible to changes via regular aerobic exercise in rehabilitation programs. However, the effects of exercise on lungs exposed to DEP after the cessation of exercise are not clear. Therefore, the aim of this study was to evaluate the effects of high-intensity swimming on lung inflammation and oxidative stress in mice exposed to DEP concomitantly and after exercise cessation. Male Swiss mice were divided into 4 groups: Control (n = 12), Swimming (30 min/day) (n = 8), DEP (3 mg/mL-10 mu L/mouse) (n = 9) and DEP+Swimming (n = 8). The high-intensity swimming was characterized by an increase in blood lactate levels greater than 1 mmoL/L between 10th and 30th minutes of exercise. Twenty-four hours after the final exposure to DEP, the anesthetized mice were euthanized, and we counted the number of total and differential inflammatory cells in the bronchoalveolar fluid (BALF), measured the lung homogenate levels of IL-1 beta, TNF-alpha, IL-6, INF-(sic), IL-10, and IL-1ra using ELISA, and measured the levels of glutathione, non-protein thiols (GSH-t and NPSH) and the antioxidant enzymes catalase and glutathione peroxidase (GPx) in the lung. Swimming sessions decreased the number of total cells (p<0.001), neutrophils and lymphocytes (p<0.001; p<0.05) in the BALF, as well as lung levels of IL-1 beta (p = 0.002), TNF-alpha (p = 0.003), IL-6 (p = 0.0001) and IFN-(sic) (p = 0.0001). However, the levels of IL-10 (p = 0.01) and IL-1ra (p = 0.0002) increased in the swimming groups compared with the control groups, as did the CAT lung levels (p = 0.0001). Simultaneously, swimming resulted in an increase in the GSH-t and NPSH lung levels in the DEP group (p = 0.0001 and p<0.002). We concluded that in this experimental model, the high-intensity swimming sessions decreased the lung inflammation and oxidative stress status during DEP-induced lung inflammation in mice.
conferenceObject Effects of swimming on lung inflammation in ovalbumin-sensitized and challenged mice(2012) BRUGGEMANN, Thayse Regina; AVILA, Leonardo Cesar Melo; FORTKAMP, Barbara; SILVA, Morgana Duarte da; BOBINSKI, Franciane; MAZZARDO-MARTINS, Leidiane; MARTINS, Daniel Fernandes; SANTOS, Adair Roberto Soares; HIZUME, Deborah de CamargoconferenceObject Effects of swimming on lung inflammation and oxidative stress in diesel exposed mice(2012) AVILA, Leonardo Cesar Melo; BRUGGEMANN, Thayse Regina; FORTKAMP, Barbara; SILVA, Morgana Duarte da; OLIVEIRA, Regiane Carvalho; MARTINS, Daniel Fernandes; MAZZARDO-MARTINS, Leidiane; SOUZA, Luiz Felipe; DAFRE, Alcir; SANTOS, Adair Roberto Soares; HIZUME, Deborah de Camargo