THAIS SABATO ROMANO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Emergence of colistin resistance in the largest university hospital complex of Sao Paulo, Brazil, over five years(2017) ROSSI, Flavia; GIRARDELLO, Raquel; CURY, Ana Paula; GIOIA, Thais Sabato Romano Di; ALMEIDA JR., Joao Nobrega de; DUARTE, Alberto Jose da SilvaColistin resistance involving Gram-negative bacilli infections is a challenge for health institutions around of the world. Carbapenem-resistance among these isolates makes colistin the last therapeutic option for this treatment. Colistin resistance among Enterobacteriaceae, Acinetobacter spp., and Pseudomonas spp. was evaluated between 2010 and 2014 years, at Hospital das Clinicas, Sao Paulo, Brazil. Over five years 1346 (4.0%) colistin resistant Gramnegative bacilli were evaluated. Enterobacteriaceae was the most frequent (86.1%) pathogen isolated, followed by Acinetobacter spp. (7.6%), and Pseudomonas spp. (6.3%). By temporal analysis there was a trend for an increase of colistin resistance among Enterobacteriaceae, but not among non-fermentative isolates. Among 1346 colistin resistant isolates, carbapenem susceptibility was observed in 21.5%. Colistin resistance in our hospital has been alarmingly increased among Klebsiella pneumoniae isolates in both KPC positive and negative, thus becoming a therapeutic problem. (C) 2016 Sociedade Brasileira de Infectologia.
- Transferable Vancomycin Resistance in a Community-Associated MRSA Lineage(2014) ROSSI, Flavia; DIAZ, Lorena; WOLLAM, Aye; PANESSO, Diana; ZHOU, Yanjiao; RINCON, Sandra; NARECHANIA, Apurva; XING, Galen; GIOIA, Thais S. R. Di; DOI, Andre; TRAN, Truc T.; REYES, Jinnethe; MUNITA, Jose M.; CARVAJAL, Lina P.; HERNANDEZ-ROLDAN, Alejandra; BRANDAO, Denise; HEIJDEN, Inneke Marie van der; MURRAY, Barbara E.; PLANET, Paul J.; WEINSTOCK, George M.; ARIAS, Cesar A.We report the case of a patient from Brazil with a bloodstream infection caused by a strain of methicillin-resistant Staphylococcus aureus (MRSA) that was susceptible to vancomycin (designated BR-VSSA) but that acquired the vanA gene cluster during antibiotic therapy and became resistant to vancomycin (designated BR-VRSA). Both strains belong to the sequence type (ST) 8 community-associated genetic lineage that carries the staphylococcal chromosomal cassette mec (SCCmec) type IVa and the S. aureus protein A gene (spa) type t292 and are phylogenetically related to MRSA lineage USA300. A conjugative plasmid of 55,706 bp (pBRZ01) carrying the vanA cluster was identified and readily transferred to other staphylococci. The pBRZ01 plasmid harbors DNA sequences that are typical of the plasmid-associated replication genes rep24 or rep21 described in community-associated MRSA strains from Australia (pWBG745). The presence and dissemination of community-associated MRSA containing vanA could become a serious public health concern.
- Evaluation of the MALDI-TOF VITEK MS (TM) system for the identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis from bloodstream infections(2014) ALMEIDA JUNIOR, Joao Nobrega de; SOUZA, Leticia Bonato de; MOTTA, Adriana Lopes; ROSSI, Flavia; GIOIA, Thais Sabato Romano Di; BENARD, Gil; NEGRO, Gilda Maria Barbaro DelTwenty-nine Candida parapsilosis, seventeen Candida orthopsilosis and two Candida metapsilosis bloodstream isolates were submitted for identification by VITEK-MS (TM) mass spectrometer. Four isolates, two C. orthopsilosis and two C. metapsilosis, were not identified. Inclusion of Superspectra of both species in this database is required to improve its discrimination power.
- Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis(2019) TANAKA, Tatiana; FERREIRA, Bruno Fortaleza de Aquino; OLIVEIRA, Luiza Manhezi Shin de; KATO, Juliana Mika; GIOIA, Thais Sabato Romano Di; ROSSI, Flavia; NAKASHIMA, Yoshitaka; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce Hisae; ALMEIDA JUNIOR, Joao Nobrega deOBJECTIVE: To report our experience using conventional culture methods (CM) and pediatric blood culture bottles (PBCBs) for vitreous sample culture of acute postoperative endophthalmitis. METHODS: A retrospective study was conducted at the Department of Ophthalmology, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, BR, from January 2010 to December 2015, and it included 54 patients with clinically suspected acute postoperative endophthalmitis. Vitreous samples were obtained by vitreous tap or vitrectomy. Samples from January 2010 to December 2011 were cultivated in CM, whereas samples from January 2012 to December 2015 were inoculated in PBCBs. The measured outcome was the yield of positive cultures. RESULTS: Twenty cases were included in the CM group, and 34 cases were included in the PBCB group. The yield of positive cultures in PBCBs (64.7%) was significantly higher than that in conventional CM (35%, p=0.034). Staphylococcus epidermidis and Streptococcus viridans were the two most commonly found agents. CONCLUSION: PBCBs can be used successfully in clinically suspected endophthalmitis. The method showed a higher yield of positive cultures than the conventional method. This technique appears to have several advantages over the traditional method: it saves time, as only one medium needs to be inoculated; transportation to a laboratory is easier than in the traditional method, and there is no need to maintain a supply of fresh agar media. The use of PBCBs may be recommended as the primary method for microbiological diagnosis and is especially suitable for office settings and remote clinics.
- Colistin susceptibility testing and Vitek-2 (TM): is it really useless?(2018) GIRARDELLO, Raquel; CURY, Ana Paula; FRANCO, Maria Renata Gomes; GIOIA, Thais Romano Di; ALMEIDA JR., Joao Nobrega de; ARAUJO, Maria Rita Elmor de; DUARTE, Alberto Jose da Silva; ROSSI, Flavia
- Pseudooutbreak of rapidly growing mycobacteria due to Mycobacterium abscessus subsp bolletii in a digestive and respiratory endoscopy unit caused by the same clone as that of a countrywide outbreak(2016) GUIMARAES, Thais; CHIMARA, Erica; PRADO, Gladys Villas Boas do; FERRAZOLI, Lucilaine; CARVALHO, Natalia Garcia Fernandes; SIMEAO, Fernanda Cristina dos Santos; SOUZA, Andreia Rodrigues de; COSTA, Christiane A. R.; NIERO, Cristina Viana; BRIANESI, Urze Adomaitis; GIOIA, Thais Romano di; GOMES, Laura Maria Brasileiro; SPADAO, Fernanda de Souza; SILVA, Maria das Gracas; MOURA, Eduardo Guimaraes Hourneaux de; LEVIN, Anna S.Background: The nontuberculous mycobacteria (NTM) are widely spread. In Brazil, 2,520 cases of rapidly growing mycobacteria (RGM) infections after medical procedures were reported, with 5.4% of cases related to nonsurgical invasive procedures and with an occurrence of 1 clone (BRA100) of Mycobacterium abscessus subsp bolletii. Objective: To describe a pseudooutbreak of M abscessus subsp bolletii in an endoscopy and bronchoscopy unit. Methods: The alert for a pseudooutbreak was given when 3 patients, in the same week, had a positive bronchoalveolar lavage culture for M abscessus subsp bolletii. The patients had no symptoms/signs of mycobacterial infection; thus, contamination of bronchoscopes was suspected. Samples for culturing were collected from bronchoscopes, digestive endoscopes, automated disinfection machines, and the water supply. Clinical samples were identified by polymerase chain reaction restriction-enzyme analysis (PRA) of the hsp65 gene and their pulsed-field gel electrophoresis pattern was compared with environmental samples. Results: The investigation demonstrated a contamination of bronchoscopes, digestive endoscopes, and disinfection machines. Molecular typing demonstrated that all strains belonged to the same clone (MAB01), identical to clone BRA100. Discussion: Cross-transmission due to poor disinfection as well as resistance to glutaraldehyde may play roles in the spread of MAB01 M abscessus subsp bolletii, which may have a unique resistance to the environment and adaption to human hosts. However the water supply may have played a role. Attention is needed to ensure the quality of water used to rinse disinfected equipment.
conferenceObject Trends in pathogens and antibiotic resistance of corneal culture isolates in infectious keratitis in Sao Paulo, Brazil, over a 5-year period(2019) OLIVEIRA, Luiza Manhezi Shin de Shin de; TANAKA, Tatiana; KATO, Juliana Mika; ROSSI, Flavia; ALMEIDA JUNIOR, Joao Nobrega de; GIOIA, Thais Romano Sabato di; GIGLIO, Veronica Bresciani; ALVES, Milton Ruiz; SANTO, Ruth Miyuki