KAREN LUCASECHI LOPES JANG
Projetos de Pesquisa
Unidades Organizacionais
LIM/08 - Laboratório de Anestesiologia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Maternal high-sodium intake alters the responsiveness of the renin-angiotensin system in adult offspring(2012) RAMOS, Debora R.; COSTA, Nauilo L.; JANG, Karen L. L.; OLIVEIRA, Ivone B.; SILVA, Alexandre A. da; HEIMANN, Joel C.; FURUKAWA, Luzia N. S.Aims: The goal of the current study was to evaluate the impact of maternal sodium intake during gestation on the systemic and renal renin-angiotensin-aldosterone-system (RAAS) of the adult offspring. Main methods: Female Wistar rats were fed high- (HSD-8.0% NaCl) or normal-sodium diets (NSD-1.3% NaCl) from 8 weeks of age until the delivery of their first litter. After birth, the offspring received NSD. Tail-cuff blood pressure (TcBP) was measured in the offspring between 6 and 12 weeks of age. At 12 weeks of age, the offspring were subjected to either one week of HSD or low sodium diet (LSD-0.1 6%NaCl) feeding to evaluate RAAS responsiveness or to acute saline overload to examine sodium excretory function. Plasma (PRA) and renal renin content (RRC), serum aldosterone (ALDO) levels, and renal cortical and medullary renin mRNA expression levels were evaluated at the end of the study. Key findings: TcBP was higher among dams fed HSD, but no TcBP differences were observed among the offspring. Male offspring, however, exhibited increased TcBP after one week of HSD feeding, and this effect was independent of maternal diet. Increased RAAS responsiveness to the HSD and LSD was also observed in male offspring. The baseline levels of PRA. ALDO, and cortical and medullary renin gene expression were lower but the RRC levels were higher among HSD-fed male offspring (HSDoff). Conversely, female HSDoff showed reduced sodium excretion 4 h after saline overload compared with female NSDoff. Significance: High maternal sodium intake is associated with gender-specific changes in RAAS responsiveness among adult offspring.
conferenceObject Fine particulate matter in the inspired air within the limits recommended by WHO alters placental structure(2014) SOTO, Sonia; LOPES, Karen; VERAS, Mariana; HEIMANN, JoelconferenceObject Effects of exposure do air pollution on placental structure(2013) SOTO, Sonia de Fatima; LOPES, Karen; VERAS, Mariana Matera; HEIMANN, JoelconferenceObject Mitochondria function and structure in brown adipose tissue is influenced by maternal low salt intake during the perinatal period(2012) LOPES, Karen Lucasechi; SANTOS, Elisabete Alcantara Dos; FURUKAWA, Luzia Naoko Shinohara; HEIMANN, Joel Claudio- Isolated total RNA and protein are preserved after thawing for more than twenty-four hours(2012) OLIVEIRA, Ivone Braga de; RAMOS, Debora Rothstein; LOPES, Karen Lucasechi; SOUZA, Regiane Machado de; HEIMANN, Joel Claudio; FURUKAWA, Luzia Naoko ShinoharaOBJECTIVE: The preservation of biological samples at a low temperature is important for later biochemical and/or histological analyses. However, the molecular viability of thawed samples has not been studied sufficiently in depth. The present study was undertaken to evaluate the viability of intact tissues, tissue homogenates, and isolated total RNA after defrosting for more than twenty-four hours. METHODS: The molecular viability of the thawed samples (n = 82) was assessed using the A260/A280 ratio, the RNA concentration, the RNA integrity, the level of intact mRNA determined by reverse transcriptase polymerase chain reaction, the protein level determined by Western blotting, and an examination of the histological structure. RESULTS: The integrity of the total RNA was not preserved in the thawed intact tissue, but the RNA integrity and level of mRNA were perfectly preserved in isolated defrosted samples of total RNA. Additionally, the level of beta-actin protein was preserved in both thawed intact tissue and homogenates. CONCLUSION: Isolated total RNA does not undergo degradation due to thawing for at least 24 hours, and it is recommended to isolate the total RNA as soon as possible after tissue collection. Moreover, the protein level is preserved in defrosted tissues.
conferenceObject FINE PARTICULATE MATTER IN THE AIR WITHIN THE LIMITS RECOMMENDED BY WHO ALTERS PLACENTAL STRUCTURE(2014) SOTO, Sonia; LOPES, Karen; OLIVEIRA, Ivone; SOUZA, Regiane; MARCHESI, Guilherme; VERAS, Mariana; HEIMANN, JoelconferenceObject Low salt intake is associated with maternal obesity before and during pregnancy(2013) TRESOLDI, Michelle Soto; HEIMANN, Joel Claudio; LOPES, Karen LucasechiObjective: To evaluate body weight, adipose tissue mass, serum leptin and mean blood pressure in females fed with different amounts of sodium chloride in three moments of the perinatal period: before (B) and at the end of pregnancy (P) and at the end of lactation (L). Methods: Female Wistar rats were fed low (LS: 0.15%), normal (NS: 1.3%) or high (HS: 8%) salt diet since 8 weeks of age. Mating was induced at 12 weeks of age. The mass of white adipose tissue (WAT, g/100g), brown adipose tissue (BAT, g/100g), body weight (BW, g), serum leptin (SL, μg/mL) by radioimmunoassay, intra-arterial mean blood pressure (BP, mmHg) and adiposity index (AI=WAT/BW x 100, %) were evaluated. Results (p<0.05, values represented by mean±SEM, n=4 to 10/group): In LS fed rats, BAT and SL were lower in L (BAT: 0.11±0.011; LS: 3.6±1.1) compared with B (BAT: 0.18±0.013; LS: 7.7±1.0) and P (BAT: 0.15±0.012; SL: 7.8±1.1). Also in LS fed rats, AI was lower in L (6.1±0.5) compared with B (8.1±0.6) and P (7.8±0.5). In L, BP was higher in HS (137.1±4.9) compared with NS (119.1±2.4) and LS (111.7±7.5). The BW was lower in HS (258.0±6.0) compared with NS (329.0±26.5) and LS (306.5±19.6) group. Conclusions: The amount of dietary salt consumption during the perinatal period influences maternal energy metabolism and blood pressure.