VALERIA CASSETTARI
Projetos de Pesquisa
Unidades Organizacionais
SCPACIN-62, Hospital Universitário
3 resultados
Resultados de Busca
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- A rare case of spontaneous bladder perforation with osteomyelitis and paravesical abscess due to CA-MRSA clone USA100/ST5(2022) UGINO, Ricardo Torres da Silveira; CASSETTARI, Valeria; MARTINES, Brenda Margatho Ramos; LARENAS, Ricardo Iturbe; MELOCCO, Gregory; FUGITA, Oscar Eduardo HidetoshiSpontaneous rupture of the urinary bladder represents a rare condition that may complicate with paravesical abscess. Although Staphylococcus aureus may be a part of the intestinal tract flora, it is an unusual cause of abdominal or pelvic abscesses. We report the case of a male patient with spontaneous bladder perforation with osteomyelitis and paravesical abscess, secondary to community-acquired methicillin-resistant Staphylococcus aureus infection.
- Genomic insights of international clones of Haemophilus influenzae causing invasive infections in vaccinated and unvaccinated infants(2021) CARDOSO, Brenda; FONTANA, Herrison; ESPOSITO, Fernanda; CERDEIRA, Louise; SANTOS, Silvia R.; YOSHIOKA, Cristina R. M.; SILVEIRA, Isa R. da; CASSETTARI, Valeria; LINCOPAN, NiltonThe emergence of invasive Haemophilus influenzae infections in vaccinated patient is a public health concern. We have investigated the genomic basis of invasiveness and possible vaccine failure in H. infiuenzae causing invasive disease in vaccinated and unvaccinated children in Brazil. Three H. infiuenzae strains isolated from blood cultures of pediatric patients were sequenced. Serotype, MLST, resistome and virulome were predicted using bioinformatic tools, whereas single nucleotide polymorphisms (SNPs) analysis of cap loci and the presence of the putative virulence-enhancing IS1016-bexA partial deletion were predicted in silico. Infections were caused by H. influenzae type a (Hia), type b (Hib) and nontypeable (NTHi), belonging to international high-risk clones of sequence types ST23, ST6 and ST368, respectively, which have been identified in North American, European and Asian countries. Convergence of ampicillin resistance and virulence in Hib-ST6 was supported by bla(TEM-1B) and deletion in the bexA gene, whereas presence of SNPs in the cap-b locus was associated with antigenic modifications of the capsule structure. Hia-ST23 and NTHi-ST368 strains carried galU, IpsA, opsX, rfaF, iga1, IgtC and lic1/lic2 virulence genes, associated with colonization, adaptation and damage to the lung, or invasiveness. In summary, deletion in the bexA gene and presence of SNPs in the cap locus of Hib could be contributing to invasive disease and possible vaccine failure in pediatric patients, whereas serotype replacement of Hib with type ""a"" and NTHi strains denotes the ability of non-vaccine serotypes to recolonize vaccinated patients. Finally, the dissemination of international high-risk clones of H. influenzae emphasizes the importance of monitoring changes in the molecular epidemiology of invasive H. influenzae disease.
- Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection(2017) MOURA, Quezia; ESPOSITO, Fernanda; FERNANDES, Miriam R.; ESPINOZA-MUNOZ, Maria; SOUZA, Tiago A.; SANTOS, Silvia R.; CERDEIRA, Louise; CASSETTARI, Valeria; LINCOPAN, NiltonThe emergence of hypervirulent Klebsiella pneumoniae (hvKP) with multidrug resistance (MDR) profile is a worrisome public health issue. We report the first draft genome sequence of a hypermucoviscous (positive string test) and MDR K. pneumoniae serotype K19, belonging to ST29, isolated from human infection. This strain harboured multiple antimicrobial resistance genes, including blaCTX-M-15, besides yersiniabactin and type 3 fimbriae virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that K. pneumoniae K19/ST29 killed 100% of the larvae at 24 h post-infection, in a similar way to the known hypermucoviscous hvKP K1/ST23 lineage.