HELOISA JULIANA ZABEU ROSSI COSTA
Projetos de Pesquisa
Unidades Organizacionais
LIM/32 - Laboratório de Otorrinolaringologia, Hospital das Clínicas, Faculdade de Medicina
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article Assessment of a neurophysiological model of the mandibular branch of the facial nerve in rats by electromyography (vol 121, pg 179, 2012)(2012) SALOMONE, R.; COSTA, H. J. Z. R.; RODRIGUES, J. R. F.; SILVA, S. M. Reis e; OVANDO, P. C.; BENTO, R. F.- Polyethylene Glycol fusion associated with anti-oxidants: A new promise in the treatment of traumatic paralysis (vol 40, pg 1489, 2018)(2018) SALOMONE, Raquel; JACOMO, Alfredo Luiz; BENTO, Ricardo F.; NASCIMENTO, Silvia Bona do; LEZIROVITZ, Karina; HOJAIJ, Flavio Carneiro; COSTA, Heloisa J. Z. R.
- Mandibular Branch of the Facial Nerve in Wistar Rats: New Experimental Model to Assess Facial Nerve Regeneration(2014) BENTO, Ricardo Ferreira; SALOMONE, Raquel; NASCIMENTO, Silvia Bona do; FERREIRA, Ricardo Jose Rodriguez; SILVA, Ciro Ferreira da; COSTA, Heloisa Juliana Zabeu RossiIntroduction The ideal animal model for nerve regeneration studies is the object of controversy, because all models described by the literature have advantages and disadvantages. Objective To describe the histologic and functional patterns of the mandibular branch of the facial nerve of Wistar rats to create a new experimental model of facial nerve regeneration. Methods Forty-two male rats were submitted to a nerve conduction test of the mandibular branch to obtain the compound muscle action potential. Twelve of these rats had the mandibular branch surgically removed and submitted to histologic analysis (number, partial density, and axonal diameter) of the proximal and distal segments. Results There was no statistically significant difference in the functional and histologic variables studied. Conclusion These new histologic and functional standards of the mandibular branch of the facial nerve of rats establish an objective, easy, and greatly reproducible model for future facial nerve regeneration studies.
- Polyethylene glycol fusion associated with antioxidants: A new promise in the treatment of traumatic facial paralysis(2018) SALOMONE, Raquel; JACOMO, Alfredo Luiz; NASCIMENTO, Silvia Bona do; LEZIROVITZ, Karina; HOJAIJ, Flavio Carneiro; COSTA, Heloisa Juliana Zabeu Rossi; BENTO, Ricardo FerreiraBackground: Recent studies in invertebrates have taught us that early cell membrane regeneration is determinant for axonal recovery and survival after trauma. Many authors obtained extraordinary results in neural regeneration using polyethylene glycol fusion protocols, which also involved microsutures and antioxidants. Methods: Sixty rats were evaluated with functional and histological protocol after facial nerve neurotmesis. Groups A and B had their stumps coapted with microsuture after 24 hours of neurotmesis and groups C and D after 72 hours. In addition to the microstructure, groups B and D used the polyethylene glycol-fusion protocol for the modulation of the Ca12. Results: At the sixth week, the latency of group D and duration of group B was lower than groups A and C (P 5.011). The axonal diameter of the groups that used polyethylene glycol-fusion was higher than those who did not use polyethylene glycol-fusion (P 001). Conclusion: Although not providing a functional improvement, polyethylene glycolfusion slowed down demyelination.
article 13 Citação(ões) na Scopus Assessment of a Neurophysiological Model of the Mandibular Branch of the Facial Nerve in Rats by Electromyography(2012) SALOMONE, Raquel; COSTA, Heloisa Juliana Zabeu Rossi; RODRIGUES, Jose Ricardo Ferreira; SILVA, Samanta Marques Reis e; ORANDO, Patricia Camacho; BENTO, Ricardo FerreiraObjectives: Our objective was to develop an experimental model for the noninvasive and objective evaluation of facial nerve regeneration in rats using a motor nerve conduction test (electromyography). Methods: Twenty-two rats were submitted to neurophysiological evaluation using motor nerve conduction of the mandibular branch of the facial nerve to obtain the compound muscle action potentials (CMAPs). To record the CM APs, we used two needle electrodes that were inserted into the lower lip muscle of the rat. A supramaximal electrical stimulus was applied, and the values of CMAP latency, amplitude, length, area, and stimulus intensity obtained from each side were compared by use of the Wilcoxon test. Results: There was no significant difference (all p > 0.05) in latency, amplitude, duration, area, or intensity of stimuli between the two sides. The amplitudes ranged between 1.61 and 8.30 mV, the latencies between 1.03 and 1.97 ms, and the stimulus intensities between 1.50 and 2.90 mA. Conclusions: This is a noninvasive, easy, and highly reproducible method that contributes to an improvement of the techniques previously described and may contribute to future studies of the degeneration and regeneration of the facial nerve.