HELOISA JULIANA ZABEU ROSSI COSTA
Projetos de Pesquisa
Unidades Organizacionais
LIM/32 - Laboratório de Otorrinolaringologia, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
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- Polyethylene Glycol fusion associated with anti-oxidants: A new promise in the treatment of traumatic paralysis (vol 40, pg 1489, 2018)(2018) SALOMONE, Raquel; JACOMO, Alfredo Luiz; BENTO, Ricardo F.; NASCIMENTO, Silvia Bona do; LEZIROVITZ, Karina; HOJAIJ, Flavio Carneiro; COSTA, Heloisa J. Z. R.
- Combined polyglycolic acid tube and autografting versus autografting or polyglycolic acid tube alone. A comparative study of peripheral nerve regeneration in rats(2015) COSTA, Marcio Paulino; TEIXEIRA, Nuberto Hopfgartner; LONGO, Marco Vinicius Losso; GEMPERLI, Rolf; COSTA, Heloisa Juliana Zabeu RossiPURPOSE: To compare sciatic nerve regeneration in rats using three different techniques of repair. METHODS: Fifteen isogonics rats were divided into three groups according to the method used to repair a 5-mm long defect created in the sciatic nerve: autogenous graft (Group A), polyglycolic acid tube (PGAt) (Group B), and of the association of PGAt with the graft (Group C). Histological analysis, regenerated myelinated axon number count and functional analysis were used to compare after six weeks. RESULTS: There was no difference in fiber diameter and degree of myelinization presented by Groups A, B and C. Group B presented the lowest number of regenerated axons. The groups did not display any significant functional difference after walking track analysis (p<0.05). CONCLUSION: No differences between the three groups in terms of functional recovery, although there were histological differences among them.
- Quantitative histological analysis of the mandibular branch of the facial nerve in rats(2012) COSTA, Heloisa Juliana Zabeu Rossi; SALOMONE, Raquel; SILVA, Ciro Ferreira da; COSTA, Marcio Paulino; RAMOS, Beatriz Lucchetta; BENTO, Ricardo FerreiraPURPOSE: To establish a model to quantitative histological analysis of the mandibular branch of the facial nerve in rats. METHODS: Eleven Wistar rats had their right and left mandibular branches of the facial nerve surgically removed and were sacrificed afterwards. Quantitative histological analysis was performed with: a) partial number of axons; b) partial area of the transversal cut of the nerve (9000 mu m(2)); c) partial density. The averages of partial density were obtained. The statistical study was established by Wilcoxon test (p=0.05). RESULTS: In relation to density of axons, comparison between sides shows no statistically significant difference (p=0.248; p=0.533). Mean partial density of distal and proximal samples was, respectively, 0.18 +/- 0.02 and 0.19 +/- 0.02 axons/mu m(2). Comparison between proximal and distal samples shows no statistically significant difference (p=0.859; p=0.182). CONCLUSION: This study has successfully established a model to histological quantitative analysis of the mandibular branch of the facial nerve in rats.
- Polyethylene glycol fusion associated with antioxidants: A new promise in the treatment of traumatic facial paralysis(2018) SALOMONE, Raquel; JACOMO, Alfredo Luiz; NASCIMENTO, Silvia Bona do; LEZIROVITZ, Karina; HOJAIJ, Flavio Carneiro; COSTA, Heloisa Juliana Zabeu Rossi; BENTO, Ricardo FerreiraBackground: Recent studies in invertebrates have taught us that early cell membrane regeneration is determinant for axonal recovery and survival after trauma. Many authors obtained extraordinary results in neural regeneration using polyethylene glycol fusion protocols, which also involved microsutures and antioxidants. Methods: Sixty rats were evaluated with functional and histological protocol after facial nerve neurotmesis. Groups A and B had their stumps coapted with microsuture after 24 hours of neurotmesis and groups C and D after 72 hours. In addition to the microstructure, groups B and D used the polyethylene glycol-fusion protocol for the modulation of the Ca12. Results: At the sixth week, the latency of group D and duration of group B was lower than groups A and C (P 5.011). The axonal diameter of the groups that used polyethylene glycol-fusion was higher than those who did not use polyethylene glycol-fusion (P 001). Conclusion: Although not providing a functional improvement, polyethylene glycolfusion slowed down demyelination.