ANA JESSICA PINTO
Projetos de Pesquisa
Unidades Organizacionais
LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina
22 resultados
Resultados de Busca
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conferenceObject Sympathetic Overactivity and Increased Cardiovascular Responses to Muscle Metaboreflex Activation in Post-menopausal Women with Rheumatoid Arthritis(2019) PECANHA, Tiago; MEIRELES, Kamila; PINTO, Ana Jessica; REZENDE, Diego Augusto Nunes; IRAHA, Amanda Yuri; SALES, Allan Robson Kluser; BONFIGLIOLI, Karina Rossi; SA-PINTO, Ana Lucia de; LIMA, Fernando Rodrigues; LRIGOYEN, Maria Claudia; GUALANO, Bruno; ROSCHEL, HamiltonconferenceObject Macro- and microvascular endothelial dysfunction and impaired shear rate in women with rheumatoid arthritis(2022) PECANHA, T.; MEIRELES, K.; PINTO, A. J.; REZENDE, D. A. N.; MAZZOLANI, B. C.; SMAIRA, F. I.; BONFIGLIOLI, K.; SA-PINTO, A. L.; LIMA, F. R.; FORJAZ, C.; GUALANO, B.; ROSCHEL, H.- High-Intensity Interval Training Augments Muscle Carnosine in the Absence of Dietary Beta-alanine Intake(2018) PAINELLI, Vitor De Salles; NEMEZIO, Kleiner Marcio; PINTO, Ana Jessica; FRANCHI, Mariana; ANDRADE, Isabel; RIANI, Luiz Augusto; SAUNDERS, Bryan; SALE, Craig; HARRIS, Roger Charles; GUALANO, Bruno; ARTIOLI, Guilherme GianniniPurpose Cross-sectional studies suggest that training can increase muscle carnosine (MCarn), although longitudinal studies have failed to confirm this. A lack of control for dietary -alanine intake or muscle fiber type shifting may have hampered their conclusions. The purpose of the present study was to investigate the effects of high-intensity interval training (HIIT) on MCarn. Methods Twenty vegetarian men were randomly assigned to a control (CON) (n = 10) or HIIT (n = 10) group. High-intensity interval training was performed on a cycle ergometer for 12 wk, with progressive volume (6-12 series) and intensity (140%-170% lactate threshold [LT]). Muscle carnosine was quantified in whole-muscle and individual fibers; expression of selected genes (CARNS, CNDP2, ABAT, TauT, and PAT1) and muscle buffering capacity in vitro (m(in vitro)) were also determined. Exercise tests were performed to evaluate total work done, VO2max, ventilatory thresholds (VT) and LT. Results Total work done, VT, LT, VO2max, and m(in vitro) were improved in the HIIT group (all P < 0.05), but not in CON (P > 0.05). MCarn (in mmolkg(-1) dry muscle) increased in the HIIT (15.8 5.7 to 20.6 +/- 5.3; P = 0.012) but not the CON group (14.3 +/- 5.3 to 15.0 +/- 4.9; P = 0.99). In type I fibers, MCarn increased in the HIIT (from 14.4 +/- 5.9 to 16.8 +/- 7.6; P = 0.047) but not the CON group (from 14.0 +/- 5.5 to 14.9 +/- 5.4; P = 0.99). In type IIa fibers, MCarn increased in the HIIT group (from 18.8 +/- 6.1 to 20.5 +/- 6.4; P = 0.067) but not the CON group (from 19.7 +/- 4.5 to 18.8 +/- 4.4; P = 0.37). No changes in gene expression were shown. Conclusions In the absence of any dietary intake of -alanine, HIIT increased MCarn content. The contribution of increased MCarn to the total increase in m(in vitro) appears to be small.
- Changes in Eating Habits and Sedentary Behavior During the COVID-19 Pandemic in Adolescents With Chronic Conditions(2021) MAZZOLANI, Bruna Caruso; SMAIRA, Fabiana Infante; ASTLEY, Camilla; IRAHA, Amanda Yuri; PINTO, Ana Jessica; MARQUES, Isabela Gouveia; AMARANTE, Milla Cordeiro; REZENDE, Nathalia Saffioti; SIECZKOWSKA, Sofia Mendes; FRANCO, Tathiane Christine; MIRANDA, Luana Cristina do Amaral; LINDOSO, Livia; HELITO, Alberto Carame; OBA, Jane; QUEIROZ, Ligia Bruni; PEREIRA, Rosa Maria R.; ROSCHEL, Hamilton; SILVA, Clovis Artur; GUALANO, BrunoIntroduction: Among healthy adolescents, school closures and home confinement were shown to increase unhealthier eating habits and sedentary behavior. It remains unknown to which extent the pandemic has impacted the lifestyle of adolescents with chronic conditions. Thus, the aim of this study is to report on the impact of the COVID-19 outbreak on eating habits and sedentary behavior among adolescents with multiple chronic conditions (n = 347) from a tertiary, referral hospital vs. healthy peers.Methods: This observational study was conducted in Sao Paulo (Brazil) between July and October 2020, period in which a set of social distancing measures to contain the pandemic.Results: The main findings of this study were that adolescents with chronic conditions and health peers showed important changes in eating habits (e.g., more often cooking and eating in front of television than before quarantine). Also, 86.8% of adolescents with chronic conditions and 91.6% of healthy adolescents reported increasing screen time during pandemic. No major differences were observed between patients and controls.Conclusions: Adolescents with chronic conditions and healthy peers exposed to pandemic showed substantial changes in lifestyle, stressing the need for specific care to mitigate poor eating habits and excessive sedentary behavior for patients and healthy adolescents.
- Association between physical activity and immunogenicity of an inactivated virus vaccine against SARS-CoV-2 in patients with autoimmune rheumatic diseases(2022) GUALANO, Bruno; LEMES, Italo R.; SILVA, Rafael P.; PINTO, Ana J.; MAZZOLANI, Bruna C.; I, Fabiana Smaira; SIECZKOWSKA, Sofia M.; AIKAWA, Nadia E.; PASOTO, Sandra G.; MEDEIROS-RIBEIRO, Ana C.; SAAD, Carla G. S.; YUKI, Emily F. N.; SILVA, Clovis A.; SWINTON, Paul; HALLAL, Pedro C.; ROSCHEL, Hamilton; BONFA, EloisaObjectives: To investigate whether physical activity is associated with enhanced immunogenicity of a SARS-CoV-2 inactivated vaccine (Coronavac) in patients with autoimmune rheumatic diseases (ARD) (n = 898) and in nonARD (n = 197) individuals without pre-existing immunogenicity to SARS-CoV-2. Methods: This was a prospective cohort study within an open-label, single-arm, phase 4 vaccination trial. Immunogenicity was assessed after vaccination by measuring seroconversion rates of total anti-SARS-CoV-2 S1/S2 IgG (SC), geometric mean titers of anti-S1/S2 IgG (GMT), factor-increase in GMT (FI-GMT), frequency of neutralizing antibody (NAb), and median neutralizing activity. Physical activity (active being defined as > 150 min/week) and sedentary behavior (>8h/day) were assessed by questionnaire. Results: Physically active ARD patients (n = 494) were younger and less frequently used prednisone/biologics than inactive patients (n = 404). After controlling for covariates, active patients exhibited greater SC (OR: 1.4 [95%CI: 1.1-2.0]), GMT (32% [95%CI: 8.8-60) and FI-GMT (33% [95%CI: 9.6-63%]) vs. inactive. Cluster analysis (physical activity/sedentary status) revealed greater GMT (43.0% [95% CI: 11.0-84.0%) and FI-GMT (48.0% [95%CI: 14.0-92.0%]) in active/non-sedentary vs. inactive/sedentary ARD patients. A dose-response was observed, with greater benefits for the group of patients performing > 350 min/week of physical activity (OR: 1.6 [95%CI: 1.1-2.4]; 41% [95%CI: 10-80%]; 35% [95%CI: 4.3-74], for SC, GMT, and FI-GMT, respectively) vs. the least active group (<= 30 min/week). Greater SC (OR: 9.9 [95%CI: 1.1-89.0]) and GMT (26% [95% CI: 2.2-56.0%]) were observed in active vs. inactive non-ARD. Conclusions: A physically active lifestyle may enhance SARS-CoV-2 vaccine immunogenicity, a finding of particular clinical relevance for immunocompromised patients.
- Physical activity and antibody persistence 6 months after the second dose of CoronaVac in immunocompromised patients(2022) GUALANO, Bruno; LEMES, Italo Ribeiro; SILVA, Rafael Pires da; PINTO, Ana Jessica; MAZZOLANI, Bruna Caruso; SMAIRA, Fabiana Infante; SIECZKOWSKA, Sofia Mendes; AIKAWA, Nadia Emi; PASOTO, Sandra; MEDEIROS-RIBEIRO, Ana Cristina; SAAD, Carla; YUK, Emily; SILVA, Clovis; SWINTON, Paul; HALLAL, Pedro Curi; ROSCHEL, Hamilton; BONFA, EloisaThis prospective cohort study within an open-label, single-arm, phase 4 vaccination trial ( #NCT04754698) aimed to investigate the association between physical activity and persistent anti-SARS-CoV-2 antibodies 6 months after two-dose schedule of CoronaVac in autoimmune rheumatic diseases (ARD) patients (n = 748). Persistent immunogenicity 6 months after the full-course vaccination was assessed using seroconversion rates of total anti-SARS-CoV-2 S1/S2 IgG, geometric mean titers of anti-S1/S2 IgG (GMT), and frequency of positive neutralizing antibodies (NAb). Physical activity was assessed trough questionnaire. Adjusted point estimates from logistic regression models indicated that physically active patients had greater odds of seroconversion rates (OR: 1.5 [95%CI: 1.1 to 2.1]) and NAb positivity (OR: 1.5 [95%CI: 1.0 to 2.1]), and approximately 43% greater GMT (42.8% [95%CI: 11.9 to 82.2]) than inactive ones. In conclusion, among immunocompromised patients, being physically active was associated with an increment in antibody persistence through 6 months after a full-course of an inactivated SARS-CoV-2 vaccine.
- No Associations Between Physical Activity and Immunogenicity in SARS-CoV-2 Seropositive Patients With Autoimmune Rheumatic Diseases Prior to and After Vaccination(2023) SMAIRA, Fabiana Infante; MAZZOLANI, Bruna Caruso; LEMES, italo Ribeiro; SILVA, Rafael Pires da; PINTO, Ana J.; SIECZKOWSKA, Sofia M.; AIKAWA, Nadia E.; PASOTO, Sandra G.; MEDEIROS-RIBEIRO, Ana C.; SAAD, Carla G. S.; YUK, Emily F. N.; SILVA, Clovis A.; SWINTON, Paul; KUPA, Leonard de Vinci Kanda; HALLAL, Pedro C.; ROSCHEL, Hamilton; GUALANO, Bruno; BONFA, EloisaAim: To investigate the association between physical activity and immunogenicity among SARS-CoV-2 seropositive patients with autoimmune rheumatic diseases prior to and following a 2-dose schedule of CoronaVac (Sinovac inactivated vaccine). Methods: This was a prospective cohort study within an open-label, single-arm, phase 4 vaccination trial conducted in Sao Paulo, Brazil. In this substudy, only SARS-CoV-2 seropositive patients were included. Immunogenicity was assessed by seroconversion rates of total anti-SARS-CoV-2 S1/S2 immunoglobulin G (IgG), geometric mean titers of anti-S1/S2 IgG, frequency of positive neutralizing antibodies, and neutralizing activity before and after vaccination. Physical activity was assessed through a questionnaire. Model-based analyses were performed controlling for age (<60 or>_60 y), sex, body mass index (<25, 25-30, and >30 kg/m2), and use of prednisone, immunosuppressants, and biologics. Results: A total of 180 seropositive autoimmune rheumatic disease patients were included. There was no association between physical activity and immunogenicity before and after vaccination. Conclusions: This study suggests that the positive association between physical activity and greater antibody responses seen in immunocompromised individuals following vaccination is overridden by previous SARS-CoV-2 infection, and does not extend to natural immunity.
- Effect of an exercise bout before the booster dose of an inactivated SARS-CoV-2 vaccine on immunogenicity in immunocompromised patients(2022) GUALANO, Bruno; SAAD, Carla G. S.; SIECZKOWSKA, Sofia M.; LEMES, Italo Ribeiro; SILVA, Rafael Pires da; PINTO, Ana J.; MAZZOLANI, Bruna C.; I, Fabiana Smaira; GIL, Saulo; OLIVEIRA-JUNIOR, Gersiel; AIKAWA, Nadia E.; MEDEIROS-RIBEIRO, Ana C.; SILVA, Clovis A.; YUKI, Emily F. N.; PASOTO, Sandra G.; PEREIRA, Rosa Maria R.; SHINJO, Samuel K.; ANDRADE, Danieli C. O.; SAMPAIO-BARROS, Percival D.; ROSCHEL, Hamilton; BONFA, EloisaThis randomized controlled study aimed to investigate whether a single bout of exercise before the homologous booster dose of a SARS-CoV-2 inactivated vaccine could enhance immunogenicity in patients with spondyloarthritis. We selected 60 consecutive patients with spondyloarthritis (SpA). Patients assigned to the intervention group performed an exercise bout comprising three exercises. Then, they remained at rest for 1 h before vaccination. The control group remained at rest before vaccination. Immunogenicity was assessed before (Pre) and 1 mo after (Post) the booster using seropositivity rates of total anti-SARS-CoV-2 S1/S2 IgG, geometric mean titers of anti-S1/S2 IgG (GMT), frequency of neutralizing antibodies (NAb) positivity, and NAb activity. At Pre, 16 patients from the exercise group and 16 patients from the control group exhibited seropositivity for IgG (59% vs. 57.1%), and 1 mo after the booster dose, seropositivity occurred in 96% versus 100% of the cases. Only 10 patients from the exercise group and 12 patients from the control group showed positive NAb serology at Pre (37% vs. 42.8%). One month following the booster, NAb positivity was 96% versus 93%. GMT was comparable between groups at Pre. At Post, GMT increased similarly in both groups. Likewise, NAb activity was similar between groups at Pre and increased similarly in both of them as a result of the booster (47.5% vs. 39.9%). In conclusion, a single bout of exercise did not enhance immunogenicity to a homologous booster dose of an inactivated SARS-CoV-2 vaccine among patients with spondyloarthritis. NEW & NOTEWORTHY We tested the role of exercise as an adjuvant to a booster of a COVID-19 vaccine. Immunocompromised patients were immunized after an acute bout of exercise or not. Patients exhibited an excellent immunogenicity in response to
- High SARS-CoV-2 infection rate after resuming professional football in Sao Paulo, Brazil(2022) GUALANO, Bruno; BRITO, Gisele Mendes; PINTO, Ana Jessica; LEMES, Italo Ribeiro; MATOS, Luciana Diniz Nagem Janot; PINTO, Ana Lucia de Sa; LOTURCO, IrineuObjective To examine the SARS-CoV-2 infection rate in a cohort of 6500 professional athletes and staff during the 2020 football (soccer) season in Sao Paulo, Brazil. Methods This retrospective cohort study included 4269 players (87% male, age: 21.7 +/- 4.2 years) and 2231 staff (87% male, age: 42.6 +/- 11.9 years) from 122 teams (women: n=16) involved in eight leagues (women: n=2), which took place in Sao Paulo, Brazil. Between 4 July 2020 and 21 December 2020, swab samples were collected weekly (n=29 507) and tested for SARS-Cov-2 via reverse transcription-PCR by an accredited laboratory commissioned by the Sao Paulo Football Federation. We contacted the medical staff of each team with positive cases to collect information on disease severity. Results Among 662 PCR-confirmed cases, 501 were athletes and 161 were staff. The new infection rate was 11.7% and 7.2% for athletes and staff, respectively. Athletes were more susceptible to infection than staff (OR: 1.71, 95% CI: 1.42, 2.06, p<0.001), although with lower chance for moderate to severe disease (OR: 0.06, 95% CI: 0.01, 0.54, p=0.012). Six teams had >= 20 individuals testing positive for SARS-CoV-2, whereas 19 teams had >= 10 confirmed cases. Twenty-five mass outbreaks were identified (>= 5 infections within a team in a 2-week period). The prevalence of SARS-CoV-2 infections was similar in athletes and staff as the general population in Sao Paulo. Conclusion Despite weekly testing and other preventive measures, we found a high SARS-CoV-2 infection rate in athletes and staff after resuming football, which coincides with the high prevalence of infection in the community during the same period. These data may assist policy-makers and sports federations for determining if and when it is safe to resume competitions.
- Chronotropic Incompetence and Reduced Heart Rate Recovery in Rheumatoid Arthritis(2018) PECANHA, Tiago; RODRIGUES, Reynaldo; PINTO, Ana Jessica; SA-PINTO, Ana Lucia; GUEDES, Lissiane; BONFIGLIOLI, Karina; GUALANO, Bruno; ROSCHEL, HamiltonBackground/Objective Recent studies have indicated that cardiac autonomic dysfunction is an early sign of cardiovascular impairment in rheumatoid arthritis (RA). Previous studies have mainly focused on resting assessments; however, analysis of heart rate (HR) responses to exercise might provide additional information on cardiac autonomic dysfunction in this disease. Thus, we aimed to assess the HR responses during and after a maximal graded exercise test in patients with RA and healthy controls (CONs). Methods This was a cross-sectional study in which 27 female RA patients and 14 female CONs frequency matched by physical activity, age, and body mass index were compared for HR responses during and after a maximal graded exercise test. Results Rheumatoid arthritis patients showed reduced chronotropic response (94.3% 16.3% vs. 106.1% +/- 10.3%, p = 0.02) and lower HR recovery (HRR) at 30 seconds (8.6 +/- 6.7 vs. 13.4 +/- 5.2 beats/min [bpm], p = 0.02), 60 seconds (16.5 +/- 7.8 vs. 24.0 +/- 9.9 bpm, p = 0.01), 120 seconds (32.6 +/- 9.9 vs. 40.7 +/- 12.3 bpm, p = 0.03), and 180 seconds (46.5 +/- 12.6 vs. 55.5 +/- 13.4 bpm, p = 0.05) post-maximal exercise test when compared with CONs. Moreover, the prevalence of chronotropic incompetence (i.e., failure to reach 80% of the HR-predicted response) and abnormal HRR (i.e., HRR 12 bpm) were, respectively, 22.2% and 37.1% in RA patients. Conclusions Patients with RA showed reduced chronotropic response to exercise and slower postexercise HRR. These abnormal autonomic responses to exercise indicate the presence of cardiac autonomic dysfunction and increased cardiovascular risk in this population.
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