CLAUDIA VEIGA CHANG
Índice h a partir de 2011
1
Projetos de Pesquisa
Unidades Organizacionais
LIM/42 - Laboratório de Hormônios e Genética Molecular, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
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conferenceObject Congenital Hypopituitarism: Stem Cell Accumulation in Mutants with Developmental Arrest and Depletion in Mutants with Hyperplasia(2014) CHANG, Claudia Veiga; ARAUJO, Ricardo Viera; CIRQUEIRA, Cinthya Santos; PARFIENIUK, Agata; GUZZO, Mariana F.; BENEDETTI, Anna Flavia F.; SOARES, Ibere C.; MILLAN, Maria Ines Perez; CAMPER, Sally Ann; CARVALHO, Luciani R. S.bookPart Hipopituitarismo em crianças e adultos(2017) CARVALHO, Luciani Renata Silveira de; CHANG, Cláudia Veiga; CORRêA, Fernanda de Azevedo; MADEIRA, João Luiz Oliveira; GUZZO, Mariana Furieri; ARNHOLD, Ivo Jorge Prado- Differential Expression of Stem Cell Markers in Human Adamantinomatous Craniopharyngioma and Pituitary Adenoma(2017) CHANG, Claudia Veiga; ARAUJO, Ricardo Vieira; CIRQUEIRA, Cinthya Santos; CANI, Carolina Maria Gomes; MATUSHITA, Hamilton; CESCATO, Valter Angelo Sperling; FRAGOSO, Maria Candida Barisson Villares; BRONSTEIN, Marcello Delano; ZERBINI, Maria Claudia Nogueira; MENDONCA, Berenice Bilharinho; CARVALHO, Luciani RenataBackground/Aims: Although craniopharyngioma (CP) is histologically benign, it is a pituitary tumour that grows rapidly and often recurs. Adamantinomatous CP (ACP) was associated with an activating mutation in beta-catenin, and it has been postulated that pituitary stem cells might play a role in oncogenesis in human ACP. Stem cells have also been identified in pituitary adenoma. Our aim was to characterize the expression pattern of ABCG2, CD44, DLL4, NANOG, NOTCH2, POU5F1/OCT4, SOX2, and SOX9 stem cell markers in human ACP and pituitary adenoma. Methods and Results: We studied 33 patients (9 ACP and 24 adenoma) using real- time quantitative PCR (RT-qPCR) and immunohistochemistry. SOX9 was up-regulated in ACP, exhibiting positive immunostaining in the epithelium and stroma, with the highest expression in patients with recurrence. CD44 was overexpressed in ACP as confirmed by immunohistochemistry. SOX2 did not significantly differ among the tumour types. The RT-qPCR array showed an increased expression of MKI67, OCT4/POU5F1, and DLL4 in all tumours. NANOG was decreased in ACP. ABCG2 was down-regulated in most of the tumours. NOTCH2 was significantly decreased in the adenomas. Conclusion: Our results confirm the presence of stem cell markers in human pituitary tumours as well as the different expression patterns of ACP and adenoma. These findings suggest that ACP may originate from a more undifferentiated cell cluster. Additionally, SOX9 immunodetection in the stroma and the highest expression levels related to the relapse of patients suggest a contribution to the aggressive behaviour and high recurrence of this tumour type. (C) 2016 S. Karger AG, Basel.
conferenceObject Unique Effects of PROP1 and POU1F1 on Pituitary Progenitor Development, Proliferation and Differentiation(2014) MILIAN, Maria Ines Perez; BRINKMEIER, Michelle L.; CHANG, Claudia Veiga; CARVALHO, Luciani R. S.; CAMPER, Sally AnnconferenceObject Congenital Hypopituitarism: Stem Cell Accumulation in Mutants with Developmental Arrest and Depletion in Mutants with Hyperplasia(2014) CHANG, Claudia Veiga; ARAUJO, Ricardo Viera; CIRQUEIRA, Cinthya Santos; PARFIENIUK, Agata; GUZZO, Mariana F.; BENEDETTI, Anna Flavia F.; SOARES, Ibere C.; MILLAN, Maria Ines Perez; CAMPER, Sally Ann; CARVALHO, Luciani R. S.