THAIS MAUAD

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Departamento de Patologia, Faculdade de Medicina - Docente
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder

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Assunto: Air pollution ×

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Agora exibindo 1 - 10 de 23
  • article 1 Citação(ões) na Scopus
    Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles
    (2021) SERIANI, Robson; PAULA, Carla Peres de; CUNHA, Anderson Ferreira da; OLIVEIRA, Marcos Antonio de; KREMPEL, Paloma Gava; FRIAS, Daniela Perroni; NEGRI, Elnara Marcia; MAUAD, Thais; MACCHIONE, Mariangela
    Several mechanisms have been suggested to explain the adverse effects of air pollutants on airway cells. One such explanation is the presence of high concentrations of oxidants and pro-oxidants in environmental pollutants. All animal and plant cells have developed several mechanisms to prevent damage by oxidative molecules. Among these, the peroxiredoxins (PRDXs) are of interest due to a high reactivity with reactive oxygen species (ROS) through the functioning of the thioredoxin/thioredoxin reductase system. This study aimed to verify the gene expression patterns of the PRDX family in bronchial epithelial airway cells (BEAS-2B) cells exposed to diesel exhaust particles (DEPs) at a concentration of 15 mu g/mL for 1 or 2 h because this it is a major component of particulate matter in the atmosphere. There was a significant decrease in mRNA fold changes of PRDX2 (0.43 +/- 0.34; *p = 0.0220), PRDX5 (0.43 +/- 0.34; *p = 0.0220), and PRDX6 (0.33 +/- 0.25; *p = 0.0069) after 1 h of exposure to DEPs. The reduction in mRNA levels may consequently lead to a decrease in the levels of PRDX proteins, increasing oxidative stress in bronchial epithelial cells BEAS-2B and thus, negatively affecting cellular functions.
  • conferenceObject
    Influence of exposure to concentrated urban air particles on the respiratory system of (LDLr-/-) mice
    (2014) MAUAD, Thais; ALEMANY, Adair; VERAS, Mariana; JUNIOR, Gabriel Ribeiro; COSTA, Natalia; CATANOZI, Sergio; ABDALLA, Dulcineia; SALDIVA, Paulo
  • conferenceObject
    Microplastics inhalation: evidence in human lung tissue
    (2021) AMATO-LOURENCO, Luis Fernando; OLIVEIRA, Regiani Carvalho; JUNIOR, Gabriel Ribeiro; GALVAO, Luciana Dos Santos; ANDO, Romulo Augusto; MAUAD, Thais
  • article 3 Citação(ões) na Scopus
    Effects of long-standing exposure to heavy-duty diesel vehicle traffic on respiratory symptoms and airway inflammation in older adults
    (2021) CARVALHO-OLIVEIRA, Regiani; AMATO-LOURENCO, Luis F.; ALMEIDA, Pamela S.; GARCIA, Bianca B. M.; VIEIRA, William K. M.; SANTANA, Ariane; GODINHO-NETTO, Martha Cristina Motta; CARRETERO, Maria E.; SALDIVA, Paulo H. Nascimento; MAUAD, Thais
    In the present study, we evaluated the effects of chronic exposure to traffic from a heavy-duty diesel-fueled vehicle area on respiratory symptoms and airway inflammation in a nonsmoking adult and elderly population. Respiratory symptoms were evaluated by the ISAAC questionnaire (International Study of Asthma and Allergies questionnaire), and airway inflammation was assessed by fractional exhaled nitric oxide (FeNO). Forty volunteers were selected from the 112 volunteers who completed the ISAAC questionnaire for the measurement of FeNO. The FeNO population comprised seven men (six aged >= 64 years old, four aged >= 75 years old) and 32 women (27 aged >= 64 years old, nine aged >= 75 years old). FeNO levels were tracked for six months, from November 2014 to June 2015. Results: Twenty-four percent of the volunteers reported having had wheezing in the chest in the last 12 months. However, only 2.7% of the volunteers reported doctor-diagnosed asthma. There was a positive association between FeNO and pollutants in most of the study months. An increase of 1 mg m(-3) in NO2 was associated with a mean increase of 1.08 ppb in FeNO, and an increase of 1 mg m(-3) in O-3 was associated with a mean increase of 1.06 ppb in FeNO. The relative risk for NO2 ranged from 1.009 to 1.32 and that for O-3 ranged from 1.014 to 1.020. Conclusion: The frequency of respiratory symptoms was much higher than the previously described levels of 6% in the Brazilian adult population. In summary, a high frequency of respiratory symptoms and high levels of FeNO were described in an underdiagnosed adult population living very close to a heavy-duty diesel-traffic area. Older elderly adults presented greater susceptibility to airway inflammation than younger adults.
  • article 21 Citação(ões) na Scopus
    Effectiveness of traffic-related elements in tree bark and pollen abortion rates for assessing air pollution exposure on respiratory mortality rates
    (2017) CARVALHO-OLIVEIRA, Regiani; AMATO-LOURENCO, Luis F.; MOREIRA, Tiana C. L.; SILVA, Douglas R. Rocha; VIEIRA, Bruna D.; MAUAD, Thais; SAIKI, Mitiko; SALDIVA, Paulo H. Nascimento
    The majority of epidemiological studies correlate the cardiorespiratory effects of air pollution exposure by considering the concentrations of pollutants measured from conventional monitoring networks. The conventional air quality monitoring methods are expensive, and their data are insufficient for providing good spatial resolution. We hypothesized that bioassays using plants could effectively determine pollutant gradients, thus helping to assess the risks associated with air pollution exposure. The study regions were determined from different prevalent respiratory death distributions in the Sao Paulo municipality. Samples of tree flower buds were collected from twelve sites in four regional districts. The genotoxic effects caused by air pollution were tested through a pollen abortion bioassay. Elements derived from vehicular traffic that accumulated in tree barks were determined using energy-dispersive X-ray fluorescence spectrometry (EDXRF). Mortality data were collected from the mortality information program of Sao Paulo City. Principal component analysis (PCA) was applied to the concentrations of elements accumulated in tree barks. Pearson correlation and exponential regression were performed considering the elements, pollen abortion rates and mortality data. PCA identified five factors, of which four represented elements related to vehicular traffic. The elements Al, S, Fe, Mn, Cu, and Zn showed a strong correlation with mortality rates (R-2>0.87) and pollen abortion rates (R-2>0.82). These results demonstrate that tree barks and pollen abortion rates allow for correlations between vehicular traffic emissions and associated outcomes such as genotoxic effects and mortality data.
  • article 22 Citação(ões) na Scopus
    Human bronchial epithelial cells exposed in vitro to diesel exhaust particles exhibit alterations in cell rheology and cytotoxicity associated with decrease in antioxidant defenses and imbalance in pro- and anti-apoptotic gene expression
    (2016) SERIANI, Robson; SOUZA, Claudia Emanuele Carvalho de; KREMPEL, Paloma Gava; FRIAS, Daniela Perroni; MATSUDA, Monique; CORREIA, Aristides Tadeu; FERREIRA, Marcia Zotti Justo; ALENCAR, Adriano Mesquita; NEGRI, Elnara Marcia; SALDIVA, Paulo Hilario Nascimento; MAUAD, Thais; MACCHIONE, Mariangela
    Diesel exhaust particles (DEPs) from diesel engines produce adverse alterations in cells of the airways by activating intracellular signaling pathways and apoptotic gene overexpression, and also by influencing metabolism and cytoskeleton changes. This study used human bronchial epithelium cells (BEAS-2B) in culture and evaluates their exposure to DEPs (15ug/mL for 1 and 2 h) in order to determine changes to cell rheology (viscoelasticity) and gene expression of the enzymes involved in oxidative stress, apoptosis, and cytotoxicity. BEAS-2B cells exposed to DEPs were found to have a significant loss in stiffness, membrane stability, and mitochondrial activity. The genes involved in apoptosis [B cell lymphoma 2 (BCL-2 and caspase-3)] presented inversely proportional expressions (p=0.05, p=0.01, respectively), low expression of the genes involved in antioxidant responses [SOD1 (superoxide dismutase 1); SOD2 (superoxide dismutase 2), and GPx (glutathione peroxidase) (p=0.01)], along with an increase in cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) (p=0.01). These results suggest that alterations in cell rheology and cytotoxicity could be associated with oxidative stress and imbalance between pro-and antiapoptotic genes.
  • conferenceObject
    Particulate air pollution affects alveolization in a juvenile mice model of asthma
    (2014) VERAS, Mariana; TELES, Aila; COSTA, Natalia; RIBEIRO-JUNIOR, Gabriel; SALDIVA, Paulo; MAUAD, Thais
  • article 207 Citação(ões) na Scopus
    An emerging class of air pollutants: Potential effects of microplastics to respiratory human health?
    (2020) AMATO-LOURENCO, Luis Fernando; GALVAO, Luciana dos Santos; WEGER, Letty A. de; HIEMSTRA, Pieter S.; VIJVER, Martina G.; MAUAD, Thais
    It is increasingly recognized that the ubiquity of convenient single-use plastic has resulted in a global plastic pollution challenge, with substantial environmental and health consequences. Physical, chemical, and biological processes result in plastic weathering, with eventual formation of debris in the micro to nano size range. There is an increasing awareness that plastic fragments are dispersed in the air and can be inhaled by humans, which may cause adverse effects on the respiratory system and on other systems. Urban environments are often characterized by high concentrations of fine airborne dust from various sources. To date, however, there is limited information on the distribution, shape, and size of microplastics in the air in urban and other environments. In this article, we review and discuss our current understanding of the exposure characteristics of airborne plastic debris in urbanized areas, focusing on concentration, size, morphology, presence of additives and distributions of different polymers. The natural and extend data are compiled and compared to laboratory-based analyses to further our understanding of the potential adverse effects of inhaled plastic particles on human health.
  • conferenceObject
    Association between long standing heavy traffic exposure and respiratory symptoms and airway inflammation in older adults and elderly
    (2020) OLIVEIRA, Regiani Carvalho; AMATO-LOURENCO, Luis Fernando; ALMEIDA, Pamela Souza; GARCIA, Bianca B. M.; VIEIRA, Willian K. M.; GODINHO-NETTO, Martha Cristina Motta; CARRETERO, Maria E.; MAUAD, Thais; SALDIVA, Paulo H. Nascimento
  • conferenceObject
    Effects of air pollution on inflammation of respiratory system: Differences between male and female
    (2015) YOSHIZAKI, Kelly; LINO-DOS-SANTOS-FRANCO, Adriana; BRITO, Jose Mara; SANTOS, Thais Moraes Nascimento; VASCONCELOS, Perola; MAUAD, Thais; SALDIVA, Paulo Hilario Nascimento; MACCHIONE, Mariangela