Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles

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art_SERIANI_Expression_patterns_of_peroxiredoxin_genes_in_bronchial_epithelial_2021.PDF (599.14 KB)
Citações na Scopus
1
Tipo de produção
article
Data de publicação
2021
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ACADEMIC PRESS INC ELSEVIER SCIENCE
Autores
SERIANI, Robson
PAULA, Carla Peres de
CUNHA, Anderson Ferreira da
OLIVEIRA, Marcos Antonio de
KREMPEL, Paloma Gava
Citação
EXPERIMENTAL AND MOLECULAR PATHOLOGY, v.120, article ID 104641, 5p, 2021
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Several mechanisms have been suggested to explain the adverse effects of air pollutants on airway cells. One such explanation is the presence of high concentrations of oxidants and pro-oxidants in environmental pollutants. All animal and plant cells have developed several mechanisms to prevent damage by oxidative molecules. Among these, the peroxiredoxins (PRDXs) are of interest due to a high reactivity with reactive oxygen species (ROS) through the functioning of the thioredoxin/thioredoxin reductase system. This study aimed to verify the gene expression patterns of the PRDX family in bronchial epithelial airway cells (BEAS-2B) cells exposed to diesel exhaust particles (DEPs) at a concentration of 15 mu g/mL for 1 or 2 h because this it is a major component of particulate matter in the atmosphere. There was a significant decrease in mRNA fold changes of PRDX2 (0.43 +/- 0.34; *p = 0.0220), PRDX5 (0.43 +/- 0.34; *p = 0.0220), and PRDX6 (0.33 +/- 0.25; *p = 0.0069) after 1 h of exposure to DEPs. The reduction in mRNA levels may consequently lead to a decrease in the levels of PRDX proteins, increasing oxidative stress in bronchial epithelial cells BEAS-2B and thus, negatively affecting cellular functions.
Palavras-chave
Peroxiredoxins, Air pollution, Diesel exhaust particles, Oxidative stress, BEAS-2B
URI
https://observatorio.fm.usp.br/handle/OPI/41571
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/59