THAIS MAUAD
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
255 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 255
- Extracellular Matrix Composition of the Cricopharyngeus Muscle(2012) TAVARES, Raquel Aguiar; SENNES, Luiz Ubirajara; MAUAD, Thais; IMAMURA, Rui; SILVA, Luiz Fernando Ferraz da; CARRAU, Ricardo LuisThe aim of this study was to analyze the presence and distribution of total collagen, type I and type III collagen, elastic fibers, fibronectin, and versican in the endomysium of cricopharyngeus muscles from adults of various ages. The study was a cross-sectional analysis of human cricopharyngeus muscles. Twenty-seven muscles obtained from autopsies of men and women ranging in age from 28 to 92 years were analyzed with the Picrosirius method, oxidized Weigert resorcin-fuchsin, immunohistochemistry, and image analysis. Collagen had the highest density among the analyzed components. Elastic fibers surrounded each muscle cell; they were aligned longitudinally by their long axis and associated with traversing fibers, thereby forming a fiber network with embedded muscle cells. The fibronectin and versican contents varied widely among the specimens. We found no statistically significant differences between the proportion of extracellular matrix (ECM) components and factors such as gender and race. We conclude that the higher proportion of type I and type III collagen is compatible with the cricopharyngeus muscle's sphincteric behavior, and the arrangement of the elastic fibers may also contribute to the muscle's elasticity. We found no statistically significant correlation between the ECM components and age.
- Airway Smooth Muscle Hypertrophy and Hyperplasia in Asthma (commentary)(2012) JAMES, Alan; MAUAD, Thais; ABRAMSON, Michael; GREEN, Francis
article 31 Citação(ões) na Scopus Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling(2011) BISELLI, P. J. C.; LOPES, F. D. T. Q. S.; MORIYA, H. T.; RIVERO, D. H. R. F.; TOLEDO, A. C.; SALDIVA, P. H. N.; MAUAD, T.; MARTINS, A.Chronic obstructive pulmonary disease (COPD) is associated with inflammatory cell reactions, tissue destruction and lung remodeling. Many signaling pathways for these phenomena are still to be identified. We developed a mouse model of COPD to evaluate some pathophysiological mechanisms acting during the initial stage of the disease. Forty-seven 6- to 8-week-old female C57/BL6 mice (approximately 22 g) were exposed for 2 months to cigarette smoke and/or residual oil fly ash (ROFA), a concentrate of air pollution. We measured lung mechanics, airspace enlargement, airway wall thickness, epithelial cell profile, elastic and collagen fiber deposition, and by immunohistochemistry transforming growth factor-beta 1 (TGF-beta 1), macrophage elastase (MMP12), neutrophils and macrophages. We observed regional airspace enlargements near terminal bronchioles associated with the exposure to smoke or ROFA. There were also increases in airway resistance and thickening of airway walls in animals exposed to smoke. In the epithelium, we noted a decrease in the ciliated cell area of animals exposed to smoke and an increase in the total cell area associated with exposure to both smoke and ROFA. There was also an increase in the expression of TGF-beta 1 both in the airways and parenchyma of animals exposed to smoke. However, we could not detect inflammatory cell recruitment, increases in MMP12 or elastic and collagen fiber deposition. After 2 months of exposure to cigarette smoke and/or ROFA, mice developed regional airspace enlargements and airway epithelium remodeling, although no inflammation or increases in fiber deposition were detected. Some of these phenomena may have been mediated by TGF-beta 1.conferenceObject SITE-SPECIFIC REMODELLING OF THE AIRWAY SMOOTH MUSCLE LAYER IN ASTHMA(2020) NOBLE, P.; ELLIOT, J.; CAIRNCROSS, A.; MAUAD, T.; ABRAMSON, M.; MCKAY, K.; GREEN, F.; JAMES, A.conferenceObject The Acute Effect Of Sugar Cane Burning Particles In A Mouse Model Of Allergic Asthma(2014) SILVA, T. G.; GOZZI, A.; FONSECA, V. M. B.; CARVALHO, A. P. F.; MILANI, T. M. S.; BORGES, M. C.; SALDIVA, P. H. N.; MAUAD, T.- Urban forests, air quality and health: a systematic review(2019) ARANTES, B. L.; MAUAD, T.; SILVA FILHO, D. F.Cities are complex systems where many factors interconnect, therefore multidisciplinary research is crucial to understand their functional capacity and to improve the quality of life that these spaces offer. This research provides a decade of scientific literature review (2006 to 2016) about urban forest, air quality and health. This review aims to increase the discussion of the importance of multidisciplinary research in urban studies. In total we found 236 papers, from which 50 were selected for the systematic analyses. The number of multidisciplinary research papers is scarce in general, however, it has been growing in recent years. Most studies are found in the United States and China, a few in Latin America. but no studies have been reported in Africa. Few studies approached the three themes aforementioned simultaneously. which highlights the importance of strengthening a multidisciplinary approach for international urban forest research, creating results that allow integration of urban forest environments.
- Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles(2021) SERIANI, Robson; PAULA, Carla Peres de; CUNHA, Anderson Ferreira da; OLIVEIRA, Marcos Antonio de; KREMPEL, Paloma Gava; FRIAS, Daniela Perroni; NEGRI, Elnara Marcia; MAUAD, Thais; MACCHIONE, MariangelaSeveral mechanisms have been suggested to explain the adverse effects of air pollutants on airway cells. One such explanation is the presence of high concentrations of oxidants and pro-oxidants in environmental pollutants. All animal and plant cells have developed several mechanisms to prevent damage by oxidative molecules. Among these, the peroxiredoxins (PRDXs) are of interest due to a high reactivity with reactive oxygen species (ROS) through the functioning of the thioredoxin/thioredoxin reductase system. This study aimed to verify the gene expression patterns of the PRDX family in bronchial epithelial airway cells (BEAS-2B) cells exposed to diesel exhaust particles (DEPs) at a concentration of 15 mu g/mL for 1 or 2 h because this it is a major component of particulate matter in the atmosphere. There was a significant decrease in mRNA fold changes of PRDX2 (0.43 +/- 0.34; *p = 0.0220), PRDX5 (0.43 +/- 0.34; *p = 0.0220), and PRDX6 (0.33 +/- 0.25; *p = 0.0069) after 1 h of exposure to DEPs. The reduction in mRNA levels may consequently lead to a decrease in the levels of PRDX proteins, increasing oxidative stress in bronchial epithelial cells BEAS-2B and thus, negatively affecting cellular functions.
- Green Areas and PM2,5 exposure could be associated with Asthma and COPD?(2023) ALMEIDA, Francine Maria; MOREIRA, Tiana Carla Lopes; OLIVEIRA, Lucas Miranda; RIGHETTI, Renato Fraga; LOPES, Fernanda Degobbi Tenorio Quirino Santos; ALENCAR, Airlane P.; GOUVEIA, Nelson; MAUAD, Thais; LOTUFO, Paulo A.; BENSENOR, Isabela; SANTOS, Itamar Souza; TIBERIO, Iolanda Fatima Lopes Calvo
- Adenopathy and Pulmonary Infiltrates in a Japanese Emigrant in Brazil(2011) KAWASSAKI, Alexandre M.; HAGA, Hironori; DANTAS, Thiago C. A.; MUSOLINO, Rafael S.; BALDI, Bruno G.; CARVALHO, Carlos R. R.; KAIRALLA, Ronaldo A.; MAUAD, Thais
- Aerobic exercise attenuates pulmonary injury induced by exposure to cigarette smoke(2012) TOLEDO, A. C.; MAGALHAES, R. M.; HIZUME, D. C.; VIEIRA, R. P.; BISELLI, P. J. C.; MORIYA, H. T.; MAUAD, T.; LOPES, F. D. T. Q. S.; MARTINS, M. A.It has recently been suggested that regular exercise reduces lung function decline and risk of chronic obstructive pulmonary disease (COPD) among active smokers; however, the mechanisms involved in this effect remain poorly understood. The present study evaluated the effects of regular exercise training in an experimental mouse model of chronic cigarette smoke exposure. Male C57BL/6 mice were divided into four groups (control, exercise, smoke and smoke+exercise). For 24 weeks, we measured respiratory mechanics, mean linear intercept, inflammatory cells and reactive oxygen species (ROS) in bronchoalveolar lavage (BAL) fluid, collagen deposition in alveolar walls, and the expression of antioxidant enzymes, matrix metalloproteinase 9, tissue inhibitor of metalloproteinase (TIMP) 1, interleukin (IL)-10 and 8-isoprostane in alveolar walls. Exercise attenuated the decrease in pulmonary elastance (p<0.01) and the increase in mean linear intercept (p=0.003) induced by cigarette smoke exposure. Exercise substantially inhibited the increase in ROS in BAL fluid and 8-isoprostane expression in lung tissue induced by cigarette smoke. In addition, exercise significantly inhibited the decreases in IL-10, TIMP1 and CuZn superoxide dismutase induced by exposure to cigarette smoke. Exercise also increased the number of cells expressing glutathione peroxidase. Our results suggest that regular aerobic physical training of moderate intensity attenuates the development of pulmonary disease induced by cigarette smoke exposure.