Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSIQUEIRA, Lumena P. Machado
dc.contributor.authorGIMENES, Viviane M. Favero
dc.contributor.authorFREITAS, Roseli Santos de
dc.contributor.authorMELHEM, Marcia de Souza Carvalho
dc.contributor.authorBONFIETTI, Lucas Xavier
dc.contributor.authorJR, Afonso Rafael da Silva
dc.contributor.authorSANTOS, Leticia B. Souza
dc.contributor.authorMOTTA, Adriana L.
dc.contributor.authorROSSI, Flavia
dc.contributor.authorBENARD, Gil
dc.contributor.authorJR, Joao N. de Almeida
dc.date.accessioned2019-02-21T17:23:46Z
dc.date.available2019-02-21T17:23:46Z
dc.date.issued2019
dc.description.abstractCryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for C. neoformans and C. gattii genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different C. neoformans (VNI, VNII, VNIII, and VNIV) and C. gattii (VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMerieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all C. neoformans and C. gattii genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipNational Council of Technological and Scientific Development [CNPQ 421374/2016-0]
dc.identifier.citationJOURNAL OF CLINICAL MICROBIOLOGY, v.57, n.1, article ID UNSP e01282, 7p, 2019
dc.identifier.doi10.1128/JCM.01282-18
dc.identifier.eissn1098-660X
dc.identifier.issn0095-1137
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/30836
dc.language.isoeng
dc.publisherAMER SOC MICROBIOLOGYeng
dc.relation.ispartofJournal of Clinical Microbiology
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright AMER SOC MICROBIOLOGYeng
dc.subjectCryptococcus gattiieng
dc.subjectCryptococcus neoformanseng
dc.subjectMALDI-TOFeng
dc.subjectVITEK MSeng
dc.subjectgenotypic identificationeng
dc.subject.otherdesorption ionization-timeeng
dc.subject.otherflight mass-spectrometryeng
dc.subject.othermolecular typeseng
dc.subject.otheridentificationeng
dc.subject.othercomplexeng
dc.subject.otherivdeng
dc.subject.wosMicrobiologyeng
dc.titleEvaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypeseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalMELHEM, Marcia de Souza Carvalho:Adolfo Lutz Inst, Secao Micol, Div Tecn Biol Med, Sao Paulo, Brazil
hcfmusp.author.externalBONFIETTI, Lucas Xavier:Adolfo Lutz Inst, Secao Micol, Div Tecn Biol Med, Sao Paulo, Brazil
hcfmusp.citation.scopus12
hcfmusp.contributor.author-fmusphcLUMENA PEREIRA MACHADO SIQUEIRA
hcfmusp.contributor.author-fmusphcVIVIANE MAZO FAVERO GIMENES
hcfmusp.contributor.author-fmusphcROSELI SANTOS DE FREITAS
hcfmusp.contributor.author-fmusphcAFONSO RAFAEL DA SILVA JUNIOR
hcfmusp.contributor.author-fmusphcLETICIA BONATO SOUZA SANTOS
hcfmusp.contributor.author-fmusphcADRIANA LOPES MOTTA
hcfmusp.contributor.author-fmusphcFLAVIA ROSSI
hcfmusp.contributor.author-fmusphcGIL BENARD
hcfmusp.contributor.author-fmusphcJOAO NOBREGA DE ALMEIDA JUNIOR
hcfmusp.description.articlenumberUNSP e01282
hcfmusp.description.issue1
hcfmusp.description.volume57
hcfmusp.origemWOS
hcfmusp.origem.pubmed30429250
hcfmusp.origem.scopus2-s2.0-85059373523
hcfmusp.origem.wosWOS:000455018000014
hcfmusp.publisher.cityWASHINGTONeng
hcfmusp.publisher.countryUSAeng
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