IgG from atopic dermatitis patients induces IL-17 and IL-10 production in infant intrathymic TCD4 and TCD8 cells

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSGNOTTO, Fabio D. R.
dc.contributor.authorOLIVEIRA, Marilia G. de
dc.contributor.authorLIRA, Aline A. L.
dc.contributor.authorINOUE, Amanda H. S.
dc.contributor.authorTITZ, Tiago O.
dc.contributor.authorORFALI, Raquel L.
dc.contributor.authorBENTO-DE-SOUZA, Luciana
dc.contributor.authorSATO, Maria N.
dc.contributor.authorAOKI, Valeria
dc.contributor.authorDUARTE, Alberto J. S.
dc.contributor.authorVICTOR, Jefferson R.
dc.date.accessioned2018-07-05T18:02:30Z
dc.date.available2018-07-05T18:02:30Z
dc.date.issued2018
dc.description.abstractIntroductionOur group recently demonstrated that IgG modulates T cell cytokine production during the maturation process in the human thymus. The effects of this modulation are IgG repertoire dependent and can exert a systemic and long-term impact. ObjectiveTo investigate whether IgG from atopic dermatitis (AD) patients can modulate cytokine production of infant intrathymic TCD4 and TCD8 cells in vitro. MethodsThymic tissues were obtained from newborn children from nonatopic mothers, and thymocytes were cultured for 6 days with purified IgG from AD patients or with intravenous immunoglobulin (IVIG) or mock conditions as controls. Cells were gated as double positive T cells (TDP(-)CD4(+)CD8(+)), TCD4 cells (CD4(+)CD8(-)), or TCD8 cells (CD4(-)CD8(+)), and intracellular levels of IL-17A, IFN-, TNF-, IL-4, IL-10, and TGF- were evaluated by flow cytometry. ResultsCompared to mock and IVIG culture conditions, IgG of AD individuals induced in vitro intracellular production of IL-17 and IL-10 by intrathymic TDP, TCD4, and TCD8 cells of infants. TGF- was also detected at a higher frequency in response to AD IgG in TDP and TCD8 cells compared to mock and IVIG cultured conditions. An opposite effect was detected upon IFN- production in TCD4 cells, such that AD IgG reduced IFN- production compared to production under mock conditions but not under IVIG conditions. ConclusionIgG of AD patients can stimulate cytokine production in infant thymocytes and thus resembles the peripheral profile observed in adults. These findings suggest a novel mechanism that can contribute to AD pathogenesis.
dc.description.indexMEDLINE
dc.description.sponsorshipLaboratory of Medical Investigation-56, Medical School, University of Sao Paulo, Sao Paulo, Brazil [LIM-56 HC-FMUSP]
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP) [2015/17256-3]
dc.description.sponsorshipSao Paulo Administrative Development Foundation (FUNDAP)
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq) [115603/2015-8]
dc.identifier.citationINTERNATIONAL JOURNAL OF DERMATOLOGY, v.57, n.4, p.434-440, 2018
dc.identifier.doi10.1111/ijd.13907
dc.identifier.eissn1365-4632
dc.identifier.issn0011-9059
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/26931
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofInternational Journal of Dermatology
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY
dc.subject.othertumor-necrosis-factor
dc.subject.otheri hypersensitivity response
dc.subject.othermaternal immunization
dc.subject.otherdermatophagoides-pteronyssinus
dc.subject.otherinterleukin-10 production
dc.subject.otherallergen exposure
dc.subject.otherskin inflammation
dc.subject.othercytokine profiles
dc.subject.otherdendritic cells
dc.subject.otheroral tolerance
dc.subject.wosDermatology
dc.titleIgG from atopic dermatitis patients induces IL-17 and IL-10 production in infant intrathymic TCD4 and TCD8 cells
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalOLIVEIRA, Marilia G. de:Univ Sao Paulo, Lab Med Invest LIM 56, Div Dermatol, Sch Med, Ave Dr Eneas Carvalho Aguiar 500,3rd Floor, BR-05403000 Sao Paulo, Brazil
hcfmusp.author.externalTITZ, Tiago O.:Univ Sao Paulo, Lab Med Invest LIM 56, Div Dermatol, Sch Med, Ave Dr Eneas Carvalho Aguiar 500,3rd Floor, BR-05403000 Sao Paulo, Brazil
hcfmusp.author.externalBENTO-DE-SOUZA, Luciana:Univ Sao Paulo, Lab Med Invest LIM 56, Div Dermatol, Sch Med, Ave Dr Eneas Carvalho Aguiar 500,3rd Floor, BR-05403000 Sao Paulo, Brazil
hcfmusp.citation.scopus21
hcfmusp.contributor.author-fmusphcFABIO DA RESSURREICAO SGNOTTO
hcfmusp.contributor.author-fmusphcALINE APARECIDA DE LIMA LIRA
hcfmusp.contributor.author-fmusphcAMANDA HARUMI SABO INOUE
hcfmusp.contributor.author-fmusphcRAQUEL LEAO ORFALI
hcfmusp.contributor.author-fmusphcMARIA NOTOMI SATO
hcfmusp.contributor.author-fmusphcVALERIA AOKI
hcfmusp.contributor.author-fmusphcALBERTO JOSE DA SILVA DUARTE
hcfmusp.contributor.author-fmusphcJEFFERSON RUSSO VICTOR
hcfmusp.description.beginpage434
hcfmusp.description.endpage440
hcfmusp.description.issue4
hcfmusp.description.volume57
hcfmusp.origemWOS
hcfmusp.origem.pubmed29355930
hcfmusp.origem.scopus2-s2.0-85043487873
hcfmusp.origem.wosWOS:000427119800012
hcfmusp.publisher.cityHOBOKEN
hcfmusp.publisher.countryUSA
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